Reviewed, UniProtKB/Swiss-Prot P04830 (CDGT1_PAEMA)

Names and origin

Protein names

Recommended name:
    Cyclomaltodextrin glucanotransferase
    EC=2.4.1.19
Alternative name(s):
    Cyclodextrin-glycosyltransferase
      Short name=CGTase

Gene names

Name:

cgtM

Organism

Paenibacillus macerans (Bacillus macerans)

Taxonomic identifier

44252 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Paenibacillus

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Secreted By similarity.
Domain	May consist of two protein domains: the one in the N-terminal side cleaves the alpha-1,4-glucosidic bond in starch, and the other in the C-terminal side catalyzes other activities, including the reconstitution of an alpha-1,4-glucosidic linkage for cyclizing the maltooligosaccharide produced.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. Contains 1 CBM20 (carbohydrate binding type-20) domain. Contains 1 IPT/TIG domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate binding Inferred from electronic annotation. Source: InterPro cyclomaltodextrin glucanotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27
	Chain	28 – 714	687	Cyclomaltodextrin glucanotransferase		PRO_0000001434
Regions
	Domain	527 – 607	81	IPT/TIG
	Domain	609 – 714	106	CBM20
	Region	28 – 165	138	A1
	Region	166 – 229	64	B
	Region	230 – 434	205	A2
	Region	435 – 523	89	C
	Region	524 – 610	87	D
	Region	611 – 714	104	E
Sites
	Active site	256	1	By similarity
	Active site	285	1	By similarity
	Active site	356	1	By similarity
	Metal binding	54	1	Calcium 2 By similarity
	Metal binding	59	1	Calcium 2 By similarity
	Metal binding	60	1	Calcium 2 By similarity
	Metal binding	78	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	80	1	Calcium 2 By similarity
	Metal binding	166	1	Calcium 1 By similarity
	Metal binding	217	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	226	1	Calcium 1 By similarity
	Metal binding	260	1	Calcium 1; via carbonyl oxygen By similarity
Experimental info
	Sequence conflict	152 – 174	23	AHAHN…PADRD → LTLITSRSDRLRPQPHVSGR AGT in AAA22298. Ref.2
	Sequence conflict	183 – 184	2	GM → AL in AAA22298. Ref.2
	Sequence conflict	260 – 261	2	HM → QY in AAA22298. Ref.2
	Sequence conflict	643	1	S → T in AAA22298. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P04830-1 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: F8A9AE794D2F3054	FASTA	714	76,960
	10 20 30 40 50 60 MKSRYKRLTS LALSLSMALG ISLPAWASPD TSVDNKVNFS TDVIYQIVTD RFADGDRTNN 70 80 90 100 110 120 PAGDAFSGDR SNLKLYFGGD WQGIIDKIND GYLTGMGVTA LWISQPVENI TSVIKYSGVN 130 140 150 160 170 180 NTSYHGYWAR DFKQTNDAFG DFADFQNLID TAHAHNIKVV IDFAPNHTSP ADRDNPGFAE 190 200 210 220 230 240 NGGMYDNGSL LGAYSNDTAG LFHHNGGTDF STIEDGIYKN LYDLADINHN NNAMDAYFKS 250 260 270 280 290 300 AIDLWLGMGV DGIRFDAVKH MPFGWQKSFV SSIYGGDHPV FTFGEWYLGA DQTDGDNIKF 310 320 330 340 350 360 ANESGMNLLD FEYAQEVREV FRDKTETMKD LYEVLASTES QYDYINNMVT FIDNHDMDRF 370 380 390 400 410 420 QVAGSGTRAT EQALALTLTS RGVPAIYYGT EQYMTGDGDP NNRAMMTSFN TGTTAYKVIQ 430 440 450 460 470 480 ALAPLRKSNP AIAYGTTTER WVNNDVLIIE RKFGSSAALV AINRNSSAAY PISGLLSSLP 490 500 510 520 530 540 AGTYSDVLNG LLNGNSITVG SGGAVTNFTL AAGGTAVWQY TAPETSPAIG NVGPTMGQPG 550 560 570 580 590 600 NIVTIDGRGF GGTAGTVYFG TTAVTGSGIV SWEDTQIKAV IPKVAAGKTG VSVKTSSGTA 610 620 630 640 650 660 SNTFKSFNVL TGDQVTVRFL VNQANTNYGT NVYLVGNAAE LGSWDPNKAI GPMYNQVIAK 670 680 690 700 710 YPSWYYDVSV PAGTKLDFKF IKKGGGTVTW EGGGNHTYTT PASGVGTVTV DWQN

References

[1]	"Cyclization characteristics of cyclodextrin glucanotransferase are conferred by the NH2-terminal region of the enzyme." Fujiwara S., Kakihara H., Woo K.B., Lejeune A., Kanemoto M., Sakaguchi K., Imanaka T. Appl. Environ. Microbiol. 58:4016-4025(1992) [PubMed: 1476442] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IFO 3490 / NRRL B-388.
[2]	"Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene." Takano T., Fukuda M., Monma M., Kobayashi S., Kainuma K., Yamane K. J. Bacteriol. 166:1118-1122(1986) [PubMed: 3011735] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM 1243.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59045 Genomic DNA. Translation: CAA41773.1. M12777 Genomic DNA. Translation: AAA22298.1.
PIR	ALBSGR. S31281.
3D structure databases
HSSP	HSSP built from PDB template 1CIU based on UniProtKB P26827.
ModBase	Search...
Protein family/group databases
CAZy	CBM20. Carbohydrate-Binding Module Family 20. GH13. Glycoside Hydrolase Family 13.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR006046. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR002044. Glyco_hydro_carb-bd. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR002909. IPT_TIG_rcpt. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF00686. CBM_20. 1 hit. PF01833. TIG. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
ProDom	PD001568. Glyco_hydro_CBD. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00642. Aamy. 1 hit. SM00632. Aamy_C. 1 hit. [Graphical view]
PROSITE	PS51166. CBM20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDGT1_PAEMA

Accession

Primary (citable) accession number: P04830

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)