Reviewed, UniProtKB/Swiss-Prot P04824 (MEL1_YEAST)

Names and origin

Protein names

Recommended name:
    Alpha-galactosidase 1
    EC=3.2.1.22
Alternative name(s):
    Alpha-D-galactoside galactohydrolase 1
    Melibiase 1

Gene names

Name:

MEL1

Organism

Saccharomyces cerevisiae (Baker's yeast)

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.
Subunit structure	Homodimer.
Induction	Induced by galactose and melibiose and repressed by glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18
	Chain	19 – 471	453	Alpha-galactosidase 1		PRO_0000001013
Sites
	Active site	149	1	Nucleophile By similarity
	Active site	209	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	105	1	N-linked (GlcNAc...) Potential
	Glycosylation	175	1	N-linked (GlcNAc...) Potential
	Glycosylation	270	1	N-linked (GlcNAc...) Potential
	Glycosylation	370	1	N-linked (GlcNAc...) Potential
	Glycosylation	403	1	N-linked (GlcNAc...) Potential
	Glycosylation	413	1	N-linked (GlcNAc...) Potential
	Glycosylation	422	1	N-linked (GlcNAc...) Potential
	Glycosylation	435	1	N-linked (GlcNAc...) Potential
	Glycosylation	454	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 74		By similarity
	Disulfide bond	121 ↔ 151		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04824-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 7A2E265A6BA09DBD	FASTA	471	52,102
	10 20 30 40 50 60 MFAFYFLTAC ISLKGVFGVS PSYNGLGLTP QMGWDNWNTF ACDVSEQLLL DTADRISDLG 70 80 90 100 110 120 LKDMGYKYII LDDCWSSGRD SDGFLVADEQ KFPNGMGHVA DHLHNNSFLF GMYSSAGEYT 130 140 150 160 170 180 CAGYPGSLGR EEEDAQFFAN NRVDYLKYDN CYNKGQFGTP EISYHRYKAM SDALNKTGRP 190 200 210 220 230 240 IFYSLCNWGQ DLTFYWGSGI ANSWRMSGDV TAEFTRPDSR CPCDGDEYDC KYAGFHCSIM 250 260 270 280 290 300 NILNKAAPMG QNAGVGGWND LDNLEVGVGN LTDDEEKAHF SMWAMVKSPL IIGANVNNLK 310 320 330 340 350 360 ASSYSIYSQA SVIAINQDSN GIPATRVWRY YVSDTDEYGQ GEIQMWSGPL DNGDQVVALL 370 380 390 400 410 420 NGGSVSRPMN TTLEEIFFDS NLGSKKLTST WDIYDLWANR VDNSTASAIL GRNKTATGIL 430 440 450 460 470 YNATEQSYKD GLSKNDTRLF GQKIGSLSPN AILNTTVPAH GIAFYRLRPS S

References

[1]	"The nucleotide sequence of the yeast MEL1 gene." Liljestroem P.L. Nucleic Acids Res. 13:7257-7268(1985) [PubMed: 2997745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the inducible MEL1 gene of Saccharomyces carlsbergensis and its secreted product, alpha-galactosidase (melibiase)." Sumner-Smith M., Bozzato R.P., Skipper N., Davies R.W., Hopper J.E. Gene 36:333-340(1985) [PubMed: 3000884] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Carlsbergensis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X03102 Genomic DNA. Translation: CAA26888.1. M10604 Genomic DNA. Translation: AAA34770.1.
PIR	GBBYAG. A00897.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1369N.
IntAct	P04824. 1 interaction.
Protein family/group databases
CAZy	GH27. Glycoside Hydrolase Family 27.
Organism-specific databases
SGD	S000029662. MEL1.
Enzyme and pathway databases
BRENDA	3.2.1.22. 250.
Gene expression databases
Genevestigator	P04824.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR006215. Glyco_hydro_melibiase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF02065. Melibiase. 1 hit. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27. PR00748. MELIBIASE.
ProDom	PD002572. Glyco_hydro_GHD. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MEL1_YEAST

Accession

Primary (citable) accession number: P04824

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	October 13, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)