P04824 (MEL1_YEASX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-galactosidase 1
EC=3.2.1.22

Alternative name(s):
Alpha-D-galactoside galactohydrolase 1
Melibiase 1

Gene names

Name:

MEL1

Organism

Saccharomyces cerevisiae (Baker's yeast)

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Subunit structure	Homotetramer. Ref.3
Subcellular location	Secreted Ref.3.
Induction	Induced by galactose and melibiose and repressed by glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 18

18

Chain

19 – 471

453

Alpha-galactosidase 1

PRO_0000001013

Sites

Active site

149

1

Nucleophile Ref.3

Active site

209

1

Proton donor Ref.3

Binding site

72

1

Substrate

Binding site

73

1

Substrate

Binding site

147

1

Substrate

Binding site

205

1

Substrate

Binding site

251

1

Substrate

Amino acid modifications

Glycosylation

105

1

N-linked (GlcNAc...) Ref.3

Glycosylation

175

1

N-linked (GlcNAc...) Ref.3

Glycosylation

270

1

N-linked (GlcNAc...) Ref.3

Glycosylation

370

1

N-linked (GlcNAc...) Ref.3

Glycosylation

403

1

N-linked (GlcNAc...) Ref.3

Glycosylation

413

1

N-linked (GlcNAc...) Potential

Glycosylation

422

1

N-linked (GlcNAc...) Ref.3

Glycosylation

435

1

N-linked (GlcNAc...) Potential

Glycosylation

454

1

N-linked (GlcNAc...) Potential

Disulfide bond

42 ↔ 74

Ref.3

Disulfide bond

121 ↔ 151

Ref.3

Disulfide bond

221 ↔ 237

Ref.3

Disulfide bond

223 ↔ 230

Ref.3

Secondary structure

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471

Helix Strand Turn

Details...

Helix

37 – 40

4

Helix

46 – 58

13

Turn

89 – 91

3

Helix

99 – 105

7

Beta strand

113 – 115

3

Helix

131 – 140

10

Beta strand

145 – 149

5

Helix

160 – 176

17

Beta strand

182 – 185

4

Helix

193 – 195

3

Beta strand

202 – 205

4

Helix

239 – 246

8

Helix

273 – 285

13

Helix

296 – 298

3

Helix

301 – 307

7

Helix

310 – 316

7

Beta strand

325 – 330

6

Beta strand

343 – 347

5

Beta strand

355 – 360

6

Beta strand

367 – 371

5

Helix

373 – 376

4

Turn

377 – 379

3

Helix

385 – 388

4

Beta strand

391 – 395

5

Turn

396 – 399

4

Helix

403 – 409

7

Turn

423 – 425

3

Helix

437 – 439

3

Beta strand

453 – 457

5

Beta strand

463 – 469

7

Sequences

	Sequence		Length	Mass (Da)
	P04824 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 7A2E265A6BA09DBD	FASTA	471	52,102
	10 20 30 40 50 60 MFAFYFLTAC ISLKGVFGVS PSYNGLGLTP QMGWDNWNTF ACDVSEQLLL DTADRISDLG 70 80 90 100 110 120 LKDMGYKYII LDDCWSSGRD SDGFLVADEQ KFPNGMGHVA DHLHNNSFLF GMYSSAGEYT 130 140 150 160 170 180 CAGYPGSLGR EEEDAQFFAN NRVDYLKYDN CYNKGQFGTP EISYHRYKAM SDALNKTGRP 190 200 210 220 230 240 IFYSLCNWGQ DLTFYWGSGI ANSWRMSGDV TAEFTRPDSR CPCDGDEYDC KYAGFHCSIM 250 260 270 280 290 300 NILNKAAPMG QNAGVGGWND LDNLEVGVGN LTDDEEKAHF SMWAMVKSPL IIGANVNNLK 310 320 330 340 350 360 ASSYSIYSQA SVIAINQDSN GIPATRVWRY YVSDTDEYGQ GEIQMWSGPL DNGDQVVALL 370 380 390 400 410 420 NGGSVSRPMN TTLEEIFFDS NLGSKKLTST WDIYDLWANR VDNSTASAIL GRNKTATGIL 430 440 450 460 470 YNATEQSYKD GLSKNDTRLF GQKIGSLSPN AILNTTVPAH GIAFYRLRPS S

References

[1]	"The nucleotide sequence of the yeast MEL1 gene." Liljestroem P.L. Nucleic Acids Res. 13:7257-7268(1985) [PubMed: 2997745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the inducible MEL1 gene of Saccharomyces carlsbergensis and its secreted product, alpha-galactosidase (melibiase)." Sumner-Smith M., Bozzato R.P., Skipper N., Davies R.W., Hopper J.E. Gene 36:333-340(1985) [PubMed: 3000884] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Carlsbergensis.
[3]	"Structural analysis of Saccharomyces cerevisiae alpha-galactosidase and its complexes with natural substrates reveals new insights into substrate specificity of GH27 glycosidases." Fernandez-Leiro R., Pereira-Rodriguez A., Cerdan M.E., Becerra M., Sanz-Aparicio J. J. Biol. Chem. 285:28020-28033(2010) [PubMed: 20592022] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) ALONE AND IN COMPLEX WITH MELIBIOSE AND RAFFINOSE, SUBCELLULAR LOCATION, SUBUNIT, ACTIVE SITE, SUBSTRATE-BINDING SITES, GLYCOSYLATION AT ASN-105; ASN-175; ASN-270; ASN-370; ASN-403 AND ASN-422, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03102 Genomic DNA. Translation: CAA26888.1.
M10604 Genomic DNA. Translation: AAA34770.1.

PIR

GBBYAG. A00897.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3LRK	X-ray	1.95	A	1-471	[»]
3LRL	X-ray	2.40	A	1-471	[»]
3LRM	X-ray	2.70	A/B/C/D	1-471	[»]

ProteinModelPortal

P04824.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1369N.

IntAct

P04824. 1 interaction.

MINT

MINT-409665.

Protein family/group databases

CAZy

GH27. Glycoside Hydrolase Family 27.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

SGD

S000029662. MEL1.

Gene expression databases

Genevestigator

P04824.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_13_b.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR006215. Glyco_hydro_melibiase.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.

Pfam

PF02065. Melibiase. 1 hit.
[Graphical view]

PRINTS

PR00740. GLHYDRLASE27.
PR00748. MELIBIASE.

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MEL1_YEASX

Accession

Primary (citable) accession number: P04824

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	September 21, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program