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P04821

- CDC25_YEAST

UniProt

P04821 - CDC25_YEAST

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Protein

Cell division control protein 25

Gene

CDC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP. This protein positively controls the level of cellular cAMP at start, the stage at which the yeast cell division cycle is triggered.1 Publication

GO - Molecular functioni

  1. Ras guanyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. positive regulation of Ras GTPase activity Source: GOC
  3. Ras protein signal transduction Source: SGD
  4. regulation of cell cycle Source: SGD
  5. replicative cell aging Source: SGD
  6. traversing start control point of mitotic cell cycle Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32396-MONOMER.
ReactomeiREACT_269723. Rap1 signalling.
REACT_271315. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 25
Gene namesi
Name:CDC25
Synonyms:CTN1
Ordered Locus Names:YLR310C
ORF Names:L2142.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR310c.
SGDiS000004301. CDC25.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1452 – 147322HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. cytosol Source: SGD
  3. endoplasmic reticulum membrane Source: SGD
  4. integral component of membrane Source: UniProtKB-KW
  5. nucleus Source: SGD
  6. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15891589Cell division control protein 25PRO_0000068863Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511Phosphoserine1 Publication
Modified residuei154 – 1541Phosphoserine1 Publication
Modified residuei423 – 4231Phosphoserine1 Publication
Modified residuei580 – 5801Phosphoserine1 Publication
Modified residuei596 – 5961Phosphoserine1 Publication
Modified residuei632 – 6321Phosphoserine1 Publication
Modified residuei635 – 6351Phosphothreonine1 Publication
Modified residuei649 – 6491Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04821.
PaxDbiP04821.

Expressioni

Gene expression databases

GenevestigatoriP04821.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RAS2P011202EBI-4237,EBI-14838

Protein-protein interaction databases

BioGridi31575. 88 interactions.
DIPiDIP-2261N.
IntActiP04821. 68 interactions.
MINTiMINT-364035.
STRINGi4932.YLR310C.

Structurei

3D structure databases

ProteinModelPortaliP04821.
SMRiP04821. Positions 61-126, 1104-1543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 12871SH3PROSITE-ProRule annotationAdd
BLAST
Domaini1117 – 1247131N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini1305 – 1542238Ras-GEFPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG265981.
GeneTreeiENSGT00770000120448.
HOGENOMiHOG000111493.
InParanoidiP04821.
OMAiYATRVMH.
OrthoDBiEOG78WM2F.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04821-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDTNTSIPN TSSAREAGNA SQTPSISSSS NTSTTTNTES SSASLSSSPS
60 70 80 90 100
TSELTSIRPI GIVVAAYDFN YPIKKDSSSQ LLSVQQGETI YILNKNSSGW
110 120 130 140 150
WDGLVIDDSN GKVNRGWFPQ NFGRPLRDSH LRKHSHPMKK YSSSKSSRRS
160 170 180 190 200
SLNSLGNSAY LHVPRNPSKS RRGSSTLSAS LSNAHNAETS SGHNNTVSMN
210 220 230 240 250
NSPFSAPNDA SHITPQSSNF NSNASLSQDM TKSADGSSEM NTNAIMNNNE
260 270 280 290 300
TNLQTSGEKA GPPLVAEETI KILPLEEIEM IINGIRSNIA STWSPIPLIT
310 320 330 340 350
KTSDYKLVYY NKDLDIYCSE LPLISNSIME SDDICDSEPK FPPNDHLVNL
360 370 380 390 400
YTRDLRKNAN IEDSSTRSKQ SESEQNRSSL LMEKQDSKET DGNNNSINDD
410 420 430 440 450
DNNNENNKNE FNEAGPSSLN SLSAPDLTQN IQSRVVAPSR SSILAKSDIF
460 470 480 490 500
YHYSRDIKLW TELQDLTVYY TKTAHKMFLK ENRLNFTKYF DLISDSIVFT
510 520 530 540 550
QLGCRLMQHE IKAKSCSKEI KKIFKGLISS LSRISINSHL YFDSAFHRKK
560 570 580 590 600
MDTMNDKDND NQENNCSRTE GDDGKIEVDS VHDLVSVPLS GKRNVSTSTT
610 620 630 640 650
DTLTPMRSSF STVNENDMEN FSVLGPRNSV NSVVTPRTSI QNSTLEDFSP
660 670 680 690 700
SNKNFKSAKS IYEMVDVEFS KFLRHVQLLY FVLQSSVFSD DNTLPQLLPR
710 720 730 740 750
FFKGSFSGGS WTNPFSTFIT DEFGNATKNK AVTSNEVTAS SSKNSSISRI
760 770 780 790 800
PPKMADAIAS ASGYSANSET NSQIDLKASS AASGSVFTPF NRPSHNRTFS
810 820 830 840 850
RARVSKRKKK YPLTVDTLNT MKKKSSQIFE KLNNATGEHL KIISKPKSRI
860 870 880 890 900
RNLEINSSTY EQINQNVLLL EILENLDLSI FINLKNLIKT PSILLDLESE
910 920 930 940 950
EFLVHAMSSV SSVLTEFFDI KQAFHDIVIR LIMTTQQTTL DDPYLFSSMR
960 970 980 990 1000
SNFPVGHHEP FKNISNTPLV KGPFHKKNEQ LALSLFHVLV SQDVEFNNLE
1010 1020 1030 1040 1050
FLNNSDDFKD ACEKYVEISN LACIIVDQLI EERENLLNYA ARMMKNNLTA
1060 1070 1080 1090 1100
ELLKGEQEKW FDIYSEDYSD DDSENDEAII DDELGSEDYI ERKAANIEKN
1110 1120 1130 1140 1150
LPWFLTSDYE TSLVYDSRGK IRGGTKEALI EHLTSHELVD AAFNVTMLIT
1160 1170 1180 1190 1200
FRSILTTREF FYALIYRYNL YPPEGLSYDD YNIWIEKKSN PIKCRVVNIM
1210 1220 1230 1240 1250
RTFLTQYWTR NYYEPGIPLI LNFAKMVVSE KIPGAEDLLQ KINEKLINEN
1260 1270 1280 1290 1300
EKEPVDPKQQ DSVSAVVQTT KRDNKSPIHM SSSSLPSSAS SAFFRLKKLK
1310 1320 1330 1340 1350
LLDIDPYTYA TQLTVLEHDL YLRITMFECL DRAWGTKYCN MGGSPNITKF
1360 1370 1380 1390 1400
IANANTLTNF VSHTIVKQAD VKTRSKLTQY FVTVAQHCKE LNNFSSMTAI
1410 1420 1430 1440 1450
VSALYSSPIY RLKKTWDLVS TESKDLLKNL NNLMDSKRNF VKYRELLRSV
1460 1470 1480 1490 1500
TDVACVPFFG VYLSDLTFTF VGNPDFLHNS TNIINFSKRT KIANIVEEII
1510 1520 1530 1540 1550
SFKRFHYKLK RLDDIQTVIE ASLENVPHIE KQYQLSLQVE PRSGNTKGST
1560 1570 1580
HASSASGTKT AKFLSEFTDD KNGNFLKLGK KKPPSRLFR
Length:1,589
Mass (Da):179,092
Last modified:January 1, 1988 - v1
Checksum:iCA90053435C85465
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti497 – 4971I → Y in CAA27259. (PubMed:3011405)Curated
Sequence conflicti954 – 96310PVGHHEPFKN → LSVIMNLSR in CAA27259. (PubMed:3011405)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03579 Genomic DNA. Translation: CAA27259.1.
M15458 Genomic DNA. Translation: AAA34478.1.
U17247 Genomic DNA. Translation: AAB67360.1.
U20618 Genomic DNA. Translation: AAB64528.1.
BK006945 Genomic DNA. Translation: DAA09620.1.
PIRiA26596. RGBYC5.
RefSeqiNP_013413.1. NM_001182198.1.

Genome annotation databases

EnsemblFungiiYLR310C; YLR310C; YLR310C.
GeneIDi851019.
KEGGisce:YLR310C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03579 Genomic DNA. Translation: CAA27259.1 .
M15458 Genomic DNA. Translation: AAA34478.1 .
U17247 Genomic DNA. Translation: AAB67360.1 .
U20618 Genomic DNA. Translation: AAB64528.1 .
BK006945 Genomic DNA. Translation: DAA09620.1 .
PIRi A26596. RGBYC5.
RefSeqi NP_013413.1. NM_001182198.1.

3D structure databases

ProteinModelPortali P04821.
SMRi P04821. Positions 61-126, 1104-1543.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31575. 88 interactions.
DIPi DIP-2261N.
IntActi P04821. 68 interactions.
MINTi MINT-364035.
STRINGi 4932.YLR310C.

Proteomic databases

MaxQBi P04821.
PaxDbi P04821.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR310C ; YLR310C ; YLR310C .
GeneIDi 851019.
KEGGi sce:YLR310C.

Organism-specific databases

CYGDi YLR310c.
SGDi S000004301. CDC25.

Phylogenomic databases

eggNOGi NOG265981.
GeneTreei ENSGT00770000120448.
HOGENOMi HOG000111493.
InParanoidi P04821.
OMAi YATRVMH.
OrthoDBi EOG78WM2F.

Enzyme and pathway databases

BioCyci YEAST:G3O-32396-MONOMER.
Reactomei REACT_269723. Rap1 signalling.
REACT_271315. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.

Miscellaneous databases

NextBioi 967589.

Gene expression databases

Genevestigatori P04821.

Family and domain databases

Gene3Di 1.10.840.10. 1 hit.
InterProi IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
SMARTi SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF48366. SSF48366. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEi PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway."
    Broek D., Toda T., Michaeli T., Levin L., Birchmeier C., Zoller M., Powers S., Wigler M.
    Cell 48:789-799(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization, cloning and sequence analysis of the CDC25 gene which controls the cyclic AMP level of Saccharomyces cerevisiae."
    Camonis J.H., Kalekine M., Gondre B., Garreau H., Boy-Marcotte E., Jacquet M.
    EMBO J. 5:375-380(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The CDC25 'Start' gene of Saccharomyces cerevisiae: sequencing of the active C-terminal fragment and regional homologies with rhodopsin and cytochrome P450."
    Daniel J.H.
    Curr. Genet. 10:879-885(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 877-1589.
  6. "Domains of the Saccharomyces cerevisiae CDC25 gene controlling mitosis and meiosis."
    Munder T., Mink M., Kuentzel H.
    Mol. Gen. Genet. 214:271-277(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAINS.
  7. "The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras."
    Jones S., Vignais M.L., Broach J.R.
    Mol. Cell. Biol. 11:2641-2646(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151; SER-154; SER-580; SER-596; SER-632; THR-635 AND SER-649, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCDC25_YEAST
AccessioniPrimary (citable) accession number: P04821
Secondary accession number(s): D6VYV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 1, 1988
Last modified: November 26, 2014
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3