Cell division control protein 25 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Sequence or UniProt identifier

>sp|P04821|CDC25_YEAST Cell division control protein 25 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CDC25 PE=1 SV=1 MSDTNTSIPNTSSAREAGNASQTPSISSSSNTSTTTNTESSSASLSSSPSTSELTSIRPI GIVVAAYDFNYPIKKDSSSQLLSVQQGETIYILNKNSSGWWDGLVIDDSNGKVNRGWFPQ NFGRPLRDSHLRKHSHPMKKYSSSKSSRRSSLNSLGNSAYLHVPRNPSKSRRGSSTLSAS LSNAHNAETSSGHNNTVSMNNSPFSAPNDASHITPQSSNFNSNASLSQDMTKSADGSSEM NTNAIMNNNETNLQTSGEKAGPPLVAEETIKILPLEEIEMIINGIRSNIASTWSPIPLIT KTSDYKLVYYNKDLDIYCSELPLISNSIMESDDICDSEPKFPPNDHLVNLYTRDLRKNAN IEDSSTRSKQSESEQNRSSLLMEKQDSKETDGNNNSINDDDNNNENNKNEFNEAGPSSLN SLSAPDLTQNIQSRVVAPSRSSILAKSDIFYHYSRDIKLWTELQDLTVYYTKTAHKMFLK ENRLNFTKYFDLISDSIVFTQLGCRLMQHEIKAKSCSKEIKKIFKGLISSLSRISINSHL YFDSAFHRKKMDTMNDKDNDNQENNCSRTEGDDGKIEVDSVHDLVSVPLSGKRNVSTSTT DTLTPMRSSFSTVNENDMENFSVLGPRNSVNSVVTPRTSIQNSTLEDFSPSNKNFKSAKS IYEMVDVEFSKFLRHVQLLYFVLQSSVFSDDNTLPQLLPRFFKGSFSGGSWTNPFSTFIT DEFGNATKNKAVTSNEVTASSSKNSSISRIPPKMADAIASASGYSANSETNSQIDLKASS AASGSVFTPFNRPSHNRTFSRARVSKRKKKYPLTVDTLNTMKKKSSQIFEKLNNATGEHL KIISKPKSRIRNLEINSSTYEQINQNVLLLEILENLDLSIFINLKNLIKTPSILLDLESE EFLVHAMSSVSSVLTEFFDIKQAFHDIVIRLIMTTQQTTLDDPYLFSSMRSNFPVGHHEP FKNISNTPLVKGPFHKKNEQLALSLFHVLVSQDVEFNNLEFLNNSDDFKDACEKYVEISN LACIIVDQLIEERENLLNYAARMMKNNLTAELLKGEQEKWFDIYSEDYSDDDSENDEAII DDELGSEDYIERKAANIEKNLPWFLTSDYETSLVYDSRGKIRGGTKEALIEHLTSHELVD AAFNVTMLITFRSILTTREFFYALIYRYNLYPPEGLSYDDYNIWIEKKSNPIKCRVVNIM RTFLTQYWTRNYYEPGIPLILNFAKMVVSEKIPGAEDLLQKINEKLINENEKEPVDPKQQ DSVSAVVQTTKRDNKSPIHMSSSSLPSSASSAFFRLKKLKLLDIDPYTYATQLTVLEHDL YLRITMFECLDRAWGTKYCNMGGSPNITKFIANANTLTNFVSHTIVKQADVKTRSKLTQY FVTVAQHCKELNNFSSMTAIVSALYSSPIYRLKKTWDLVSTESKDLLKNLNNLMDSKRNF VKYRELLRSVTDVACVPFFGVYLSDLTFTFVGNPDFLHNSTNIINFSKRTKIANIVEEII SFKRFHYKLKRLDDIQTVIEASLENVPHIEKQYQLSLQVEPRSGNTKGSTHASSASGTKT AKFLSEFTDDKNGNFLKLGKKKPPSRLFR

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P04821 (CDC25_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 135. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cell division control protein 25

Gene names

Name:	CDC25
Synonyms:	CTN1
Ordered Locus Names:	YLR310C
ORF Names:	L2142.6

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1589 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes the exchange of Ras-bound GDP by GTP. This protein positively controls the level of cellular cAMP at start, the stage at which the yeast cell division cycle is triggered. Ref.7
Subcellular location	Membrane; Single-pass membrane protein Potential.
Miscellaneous	Present with 319 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain. Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Membrane
Domain	SH3 domain Transmembrane Transmembrane helix
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Ras protein signal transduction Inferred from mutant phenotype PubMed 1849965. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell cycle Inferred from mutant phenotype PubMed 3021894. Source: SGD regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro replicative cell aging Inferred from mutant phenotype PubMed 11000115. Source: SGD traversing start control point of mitotic cell cycle Inferred from mutant phenotype PubMed 3021894. Source: SGD
Cellular_component	cytosol Inferred from direct assay PubMed 18930081. Source: SGD endoplasmic reticulum membrane Inferred from direct assay PubMed 22575457. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay PubMed 18930081. Source: SGD plasma membrane Inferred from direct assay PubMed 8893498. Source: SGD
Molecular_function	Ras guanyl-nucleotide exchange factor activity Inferred from direct assay PubMed 21073870 PubMed 8206969. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1589

1589

Cell division control protein 25

PRO_0000068863

Regions

Transmembrane

1452 – 1473

Helical; Potential

Domain

58 – 128

SH3

Domain

1117 – 1247

131

N-terminal Ras-GEF

Domain

1305 – 1542

238

Ras-GEF

Amino acid modifications

Modified residue

151

Phosphoserine Ref.10 Ref.11

Modified residue

154

Phosphoserine Ref.10 Ref.11

Modified residue

222

Phosphoserine Ref.11

Modified residue

423

Phosphoserine Ref.11

Modified residue

596

Phosphoserine Ref.11

Modified residue

635

Phosphothreonine Ref.9

Modified residue

639

Phosphoserine Ref.11

Modified residue

649

Phosphoserine Ref.11

Modified residue

651

Phosphoserine Ref.11

Modified residue

768

Phosphoserine Ref.11

Experimental info

Sequence conflict

497

I → Y in CAA27259. Ref.2

Sequence conflict

954 – 963

PVGHHEPFKN → LSVIMNLSR in CAA27259. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P04821 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: CA90053435C85465
Show »

FASTA

1,589

179,092

        10         20         30         40         50         60 
MSDTNTSIPN TSSAREAGNA SQTPSISSSS NTSTTTNTES SSASLSSSPS TSELTSIRPI 

        70         80         90        100        110        120 
GIVVAAYDFN YPIKKDSSSQ LLSVQQGETI YILNKNSSGW WDGLVIDDSN GKVNRGWFPQ 

       130        140        150        160        170        180 
NFGRPLRDSH LRKHSHPMKK YSSSKSSRRS SLNSLGNSAY LHVPRNPSKS RRGSSTLSAS 

       190        200        210        220        230        240 
LSNAHNAETS SGHNNTVSMN NSPFSAPNDA SHITPQSSNF NSNASLSQDM TKSADGSSEM 

       250        260        270        280        290        300 
NTNAIMNNNE TNLQTSGEKA GPPLVAEETI KILPLEEIEM IINGIRSNIA STWSPIPLIT 

       310        320        330        340        350        360 
KTSDYKLVYY NKDLDIYCSE LPLISNSIME SDDICDSEPK FPPNDHLVNL YTRDLRKNAN 

       370        380        390        400        410        420 
IEDSSTRSKQ SESEQNRSSL LMEKQDSKET DGNNNSINDD DNNNENNKNE FNEAGPSSLN 

       430        440        450        460        470        480 
SLSAPDLTQN IQSRVVAPSR SSILAKSDIF YHYSRDIKLW TELQDLTVYY TKTAHKMFLK 

       490        500        510        520        530        540 
ENRLNFTKYF DLISDSIVFT QLGCRLMQHE IKAKSCSKEI KKIFKGLISS LSRISINSHL 

       550        560        570        580        590        600 
YFDSAFHRKK MDTMNDKDND NQENNCSRTE GDDGKIEVDS VHDLVSVPLS GKRNVSTSTT 

       610        620        630        640        650        660 
DTLTPMRSSF STVNENDMEN FSVLGPRNSV NSVVTPRTSI QNSTLEDFSP SNKNFKSAKS 

       670        680        690        700        710        720 
IYEMVDVEFS KFLRHVQLLY FVLQSSVFSD DNTLPQLLPR FFKGSFSGGS WTNPFSTFIT 

       730        740        750        760        770        780 
DEFGNATKNK AVTSNEVTAS SSKNSSISRI PPKMADAIAS ASGYSANSET NSQIDLKASS 

       790        800        810        820        830        840 
AASGSVFTPF NRPSHNRTFS RARVSKRKKK YPLTVDTLNT MKKKSSQIFE KLNNATGEHL 

       850        860        870        880        890        900 
KIISKPKSRI RNLEINSSTY EQINQNVLLL EILENLDLSI FINLKNLIKT PSILLDLESE 

       910        920        930        940        950        960 
EFLVHAMSSV SSVLTEFFDI KQAFHDIVIR LIMTTQQTTL DDPYLFSSMR SNFPVGHHEP 

       970        980        990       1000       1010       1020 
FKNISNTPLV KGPFHKKNEQ LALSLFHVLV SQDVEFNNLE FLNNSDDFKD ACEKYVEISN 

      1030       1040       1050       1060       1070       1080 
LACIIVDQLI EERENLLNYA ARMMKNNLTA ELLKGEQEKW FDIYSEDYSD DDSENDEAII 

      1090       1100       1110       1120       1130       1140 
DDELGSEDYI ERKAANIEKN LPWFLTSDYE TSLVYDSRGK IRGGTKEALI EHLTSHELVD 

      1150       1160       1170       1180       1190       1200 
AAFNVTMLIT FRSILTTREF FYALIYRYNL YPPEGLSYDD YNIWIEKKSN PIKCRVVNIM 

      1210       1220       1230       1240       1250       1260 
RTFLTQYWTR NYYEPGIPLI LNFAKMVVSE KIPGAEDLLQ KINEKLINEN EKEPVDPKQQ 

      1270       1280       1290       1300       1310       1320 
DSVSAVVQTT KRDNKSPIHM SSSSLPSSAS SAFFRLKKLK LLDIDPYTYA TQLTVLEHDL 

      1330       1340       1350       1360       1370       1380 
YLRITMFECL DRAWGTKYCN MGGSPNITKF IANANTLTNF VSHTIVKQAD VKTRSKLTQY 

      1390       1400       1410       1420       1430       1440 
FVTVAQHCKE LNNFSSMTAI VSALYSSPIY RLKKTWDLVS TESKDLLKNL NNLMDSKRNF 

      1450       1460       1470       1480       1490       1500 
VKYRELLRSV TDVACVPFFG VYLSDLTFTF VGNPDFLHNS TNIINFSKRT KIANIVEEII 

      1510       1520       1530       1540       1550       1560 
SFKRFHYKLK RLDDIQTVIE ASLENVPHIE KQYQLSLQVE PRSGNTKGST HASSASGTKT 

      1570       1580 
AKFLSEFTDD KNGNFLKLGK KKPPSRLFR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway." Broek D., Toda T., Michaeli T., Levin L., Birchmeier C., Zoller M., Powers S., Wigler M. Cell 48:789-799(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization, cloning and sequence analysis of the CDC25 gene which controls the cyclic AMP level of Saccharomyces cerevisiae." Camonis J.H., Kalekine M., Gondre B., Garreau H., Boy-Marcotte E., Jacquet M. EMBO J. 5:375-380(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The CDC25 'Start' gene of Saccharomyces cerevisiae: sequencing of the active C-terminal fragment and regional homologies with rhodopsin and cytochrome P450." Daniel J.H. Curr. Genet. 10:879-885(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 877-1589.
[6]	"Domains of the Saccharomyces cerevisiae CDC25 gene controlling mitosis and meiosis." Munder T., Mink M., Kuentzel H. Mol. Gen. Genet. 214:271-277(1988) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAINS.
[7]	"The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras." Jones S., Vignais M.L., Broach J.R. Mol. Cell. Biol. 11:2641-2646(1991) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-635, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151 AND SER-154, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151; SER-154; SER-222; SER-423; SER-596; SER-639; SER-649; SER-651 AND SER-768, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03579 Genomic DNA. Translation: CAA27259.1. M15458 Genomic DNA. Translation: AAA34478.1. U17247 Genomic DNA. Translation: AAB67360.1. U20618 Genomic DNA. Translation: AAB64528.1. BK006945 Genomic DNA. Translation: DAA09620.1.
PIR	RGBYC5. A26596.
RefSeq	NP_013413.1. NM_001182198.1.
3D structure databases
ProteinModelPortal	P04821.
SMR	P04821. Positions 61-126, 1104-1543.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2261N.
IntAct	P04821. 16 interactions.
MINT	MINT-364035.
STRING	4932.YLR310C.
Proteomic databases
PaxDb	P04821.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR310C; YLR310C; YLR310C.
GeneID	851019.
KEGG	sce:YLR310C.
Organism-specific databases
CYGD	YLR310c.
SGD	S000004301. CDC25.
Phylogenomic databases
eggNOG	NOG265981.
GeneTree	ENSGT00700000104175.
HOGENOM	HOG000111493.
OMA	SMTAIVS.
OrthoDB	EOG4VX5DB.
Gene expression databases
Genevestigator	P04821.
GermOnline	YLR310C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.840.10. 1 hit.
InterPro	IPR000651. Ras-like_Gua-exchang_fac_N. IPR019804. Ras_G-nucl-exch_fac_CS. IPR008937. Ras_GEF. IPR023578. Ras_GEF_dom. IPR001895. RasGRF_CDC25. IPR001452. SH3_domain. [Graphical view]
PANTHER	PTHR23113. PTHR23113. 1 hit.
Pfam	PF00617. RasGEF. 1 hit. PF00618. RasGEF_N. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00147. RasGEF. 1 hit. SM00229. RasGEFN. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF48366. Ras_GEF. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00720. RASGEF. 1 hit. PS50009. RASGEF_CAT. 1 hit. PS50212. RASGEF_NTER. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967589.

Entry information

Entry name

CDC25_YEAST

Accession

Primary (citable) accession number: P04821
Secondary accession number(s): D6VYV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 1, 1988
Last modified:	May 1, 2013
This is version 135 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents