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P04821 (CDC25_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 147. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell division control protein 25
Gene names
Name:CDC25
Synonyms:CTN1
Ordered Locus Names:YLR310C
ORF Names:L2142.6
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1589 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes the exchange of Ras-bound GDP by GTP. This protein positively controls the level of cellular cAMP at start, the stage at which the yeast cell division cycle is triggered. Ref.7

Subcellular location

Membrane; Single-pass membrane protein Potential.

Miscellaneous

Present with 319 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 N-terminal Ras-GEF domain.

Contains 1 Ras-GEF domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentMembrane
   DomainSH3 domain
Transmembrane
Transmembrane helix
   Molecular functionGuanine-nucleotide releasing factor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRas protein signal transduction

Inferred from mutant phenotype PubMed 1849965. Source: SGD

mitotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of Ras GTPase activity

Inferred from direct assay PubMed 21073870PubMed 8206969. Source: GOC

regulation of cell cycle

Inferred from mutant phenotype PubMed 3021894. Source: SGD

replicative cell aging

Inferred from mutant phenotype PubMed 11000115. Source: SGD

traversing start control point of mitotic cell cycle

Inferred from mutant phenotype PubMed 3021894. Source: SGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 1334534. Source: SGD

cytosol

Inferred from direct assay PubMed 18930081. Source: SGD

endoplasmic reticulum membrane

Inferred from direct assay PubMed 22575457. Source: SGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

nucleus

Inferred from direct assay PubMed 18930081. Source: SGD

plasma membrane

Inferred from direct assay PubMed 8893498. Source: SGD

   Molecular_functionRas guanyl-nucleotide exchange factor activity

Inferred from direct assay PubMed 21073870PubMed 8206969. Source: SGD

protein binding

Inferred from physical interaction PubMed 21073870. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RAS2P011202EBI-4237,EBI-14838

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15891589Cell division control protein 25
PRO_0000068863

Regions

Transmembrane1452 – 147322Helical; Potential
Domain58 – 12871SH3
Domain1117 – 1247131N-terminal Ras-GEF
Domain1305 – 1542238Ras-GEF

Amino acid modifications

Modified residue1511Phosphoserine Ref.11
Modified residue1541Phosphoserine Ref.11
Modified residue4231Phosphoserine Ref.10
Modified residue5801Phosphoserine Ref.11
Modified residue5961Phosphoserine Ref.11
Modified residue6321Phosphoserine Ref.11
Modified residue6351Phosphothreonine Ref.11
Modified residue6491Phosphoserine Ref.11

Experimental info

Sequence conflict4971I → Y in CAA27259. Ref.2
Sequence conflict954 – 96310PVGHHEPFKN → LSVIMNLSR in CAA27259. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P04821 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: CA90053435C85465

FASTA1,589179,092
        10         20         30         40         50         60 
MSDTNTSIPN TSSAREAGNA SQTPSISSSS NTSTTTNTES SSASLSSSPS TSELTSIRPI 

        70         80         90        100        110        120 
GIVVAAYDFN YPIKKDSSSQ LLSVQQGETI YILNKNSSGW WDGLVIDDSN GKVNRGWFPQ 

       130        140        150        160        170        180 
NFGRPLRDSH LRKHSHPMKK YSSSKSSRRS SLNSLGNSAY LHVPRNPSKS RRGSSTLSAS 

       190        200        210        220        230        240 
LSNAHNAETS SGHNNTVSMN NSPFSAPNDA SHITPQSSNF NSNASLSQDM TKSADGSSEM 

       250        260        270        280        290        300 
NTNAIMNNNE TNLQTSGEKA GPPLVAEETI KILPLEEIEM IINGIRSNIA STWSPIPLIT 

       310        320        330        340        350        360 
KTSDYKLVYY NKDLDIYCSE LPLISNSIME SDDICDSEPK FPPNDHLVNL YTRDLRKNAN 

       370        380        390        400        410        420 
IEDSSTRSKQ SESEQNRSSL LMEKQDSKET DGNNNSINDD DNNNENNKNE FNEAGPSSLN 

       430        440        450        460        470        480 
SLSAPDLTQN IQSRVVAPSR SSILAKSDIF YHYSRDIKLW TELQDLTVYY TKTAHKMFLK 

       490        500        510        520        530        540 
ENRLNFTKYF DLISDSIVFT QLGCRLMQHE IKAKSCSKEI KKIFKGLISS LSRISINSHL 

       550        560        570        580        590        600 
YFDSAFHRKK MDTMNDKDND NQENNCSRTE GDDGKIEVDS VHDLVSVPLS GKRNVSTSTT 

       610        620        630        640        650        660 
DTLTPMRSSF STVNENDMEN FSVLGPRNSV NSVVTPRTSI QNSTLEDFSP SNKNFKSAKS 

       670        680        690        700        710        720 
IYEMVDVEFS KFLRHVQLLY FVLQSSVFSD DNTLPQLLPR FFKGSFSGGS WTNPFSTFIT 

       730        740        750        760        770        780 
DEFGNATKNK AVTSNEVTAS SSKNSSISRI PPKMADAIAS ASGYSANSET NSQIDLKASS 

       790        800        810        820        830        840 
AASGSVFTPF NRPSHNRTFS RARVSKRKKK YPLTVDTLNT MKKKSSQIFE KLNNATGEHL 

       850        860        870        880        890        900 
KIISKPKSRI RNLEINSSTY EQINQNVLLL EILENLDLSI FINLKNLIKT PSILLDLESE 

       910        920        930        940        950        960 
EFLVHAMSSV SSVLTEFFDI KQAFHDIVIR LIMTTQQTTL DDPYLFSSMR SNFPVGHHEP 

       970        980        990       1000       1010       1020 
FKNISNTPLV KGPFHKKNEQ LALSLFHVLV SQDVEFNNLE FLNNSDDFKD ACEKYVEISN 

      1030       1040       1050       1060       1070       1080 
LACIIVDQLI EERENLLNYA ARMMKNNLTA ELLKGEQEKW FDIYSEDYSD DDSENDEAII 

      1090       1100       1110       1120       1130       1140 
DDELGSEDYI ERKAANIEKN LPWFLTSDYE TSLVYDSRGK IRGGTKEALI EHLTSHELVD 

      1150       1160       1170       1180       1190       1200 
AAFNVTMLIT FRSILTTREF FYALIYRYNL YPPEGLSYDD YNIWIEKKSN PIKCRVVNIM 

      1210       1220       1230       1240       1250       1260 
RTFLTQYWTR NYYEPGIPLI LNFAKMVVSE KIPGAEDLLQ KINEKLINEN EKEPVDPKQQ 

      1270       1280       1290       1300       1310       1320 
DSVSAVVQTT KRDNKSPIHM SSSSLPSSAS SAFFRLKKLK LLDIDPYTYA TQLTVLEHDL 

      1330       1340       1350       1360       1370       1380 
YLRITMFECL DRAWGTKYCN MGGSPNITKF IANANTLTNF VSHTIVKQAD VKTRSKLTQY 

      1390       1400       1410       1420       1430       1440 
FVTVAQHCKE LNNFSSMTAI VSALYSSPIY RLKKTWDLVS TESKDLLKNL NNLMDSKRNF 

      1450       1460       1470       1480       1490       1500 
VKYRELLRSV TDVACVPFFG VYLSDLTFTF VGNPDFLHNS TNIINFSKRT KIANIVEEII 

      1510       1520       1530       1540       1550       1560 
SFKRFHYKLK RLDDIQTVIE ASLENVPHIE KQYQLSLQVE PRSGNTKGST HASSASGTKT 

      1570       1580 
AKFLSEFTDD KNGNFLKLGK KKPPSRLFR 

« Hide

References

« Hide 'large scale' references
[1]"The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway."
Broek D., Toda T., Michaeli T., Levin L., Birchmeier C., Zoller M., Powers S., Wigler M.
Cell 48:789-799(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Characterization, cloning and sequence analysis of the CDC25 gene which controls the cyclic AMP level of Saccharomyces cerevisiae."
Camonis J.H., Kalekine M., Gondre B., Garreau H., Boy-Marcotte E., Jacquet M.
EMBO J. 5:375-380(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The CDC25 'Start' gene of Saccharomyces cerevisiae: sequencing of the active C-terminal fragment and regional homologies with rhodopsin and cytochrome P450."
Daniel J.H.
Curr. Genet. 10:879-885(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 877-1589.
[6]"Domains of the Saccharomyces cerevisiae CDC25 gene controlling mitosis and meiosis."
Munder T., Mink M., Kuentzel H.
Mol. Gen. Genet. 214:271-277(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAINS.
[7]"The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras."
Jones S., Vignais M.L., Broach J.R.
Mol. Cell. Biol. 11:2641-2646(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151; SER-154; SER-580; SER-596; SER-632; THR-635 AND SER-649, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03579 Genomic DNA. Translation: CAA27259.1.
M15458 Genomic DNA. Translation: AAA34478.1.
U17247 Genomic DNA. Translation: AAB67360.1.
U20618 Genomic DNA. Translation: AAB64528.1.
BK006945 Genomic DNA. Translation: DAA09620.1.
PIRRGBYC5. A26596.
RefSeqNP_013413.1. NM_001182198.1.

3D structure databases

ProteinModelPortalP04821.
SMRP04821. Positions 61-126, 1104-1543.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31575. 88 interactions.
DIPDIP-2261N.
IntActP04821. 68 interactions.
MINTMINT-364035.
STRING4932.YLR310C.

Proteomic databases

MaxQBP04821.
PaxDbP04821.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR310C; YLR310C; YLR310C.
GeneID851019.
KEGGsce:YLR310C.

Organism-specific databases

CYGDYLR310c.
SGDS000004301. CDC25.

Phylogenomic databases

eggNOGNOG265981.
GeneTreeENSGT00730000110221.
HOGENOMHOG000111493.
OMAYATRVMH.
OrthoDBEOG78WM2F.

Enzyme and pathway databases

BioCycYEAST:G3O-32396-MONOMER.

Gene expression databases

GenevestigatorP04821.

Family and domain databases

Gene3D1.10.840.10. 1 hit.
InterProIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48366. SSF48366. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967589.

Entry information

Entry nameCDC25_YEAST
AccessionPrimary (citable) accession number: P04821
Secondary accession number(s): D6VYV4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 1, 1988
Last modified: June 11, 2014
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families