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P04821

- CDC25_YEAST

UniProt

P04821 - CDC25_YEAST

Protein

Cell division control protein 25

Gene

CDC25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 1 (01 Jan 1988)
      Previous versions | rss
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    Functioni

    Promotes the exchange of Ras-bound GDP by GTP. This protein positively controls the level of cellular cAMP at start, the stage at which the yeast cell division cycle is triggered.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. Ras guanyl-nucleotide exchange factor activity Source: SGD

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. positive regulation of Ras GTPase activity Source: GOC
    3. Ras protein signal transduction Source: SGD
    4. regulation of cell cycle Source: SGD
    5. replicative cell aging Source: SGD
    6. traversing start control point of mitotic cell cycle Source: SGD

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32396-MONOMER.
    ReactomeiREACT_188984. PPARA activates gene expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division control protein 25
    Gene namesi
    Name:CDC25
    Synonyms:CTN1
    Ordered Locus Names:YLR310C
    ORF Names:L2142.6
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR310c.
    SGDiS000004301. CDC25.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. cytosol Source: SGD
    3. endoplasmic reticulum membrane Source: SGD
    4. integral component of membrane Source: UniProtKB-KW
    5. nucleus Source: SGD
    6. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15891589Cell division control protein 25PRO_0000068863Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei151 – 1511Phosphoserine1 Publication
    Modified residuei154 – 1541Phosphoserine1 Publication
    Modified residuei423 – 4231Phosphoserine1 Publication
    Modified residuei580 – 5801Phosphoserine1 Publication
    Modified residuei596 – 5961Phosphoserine1 Publication
    Modified residuei632 – 6321Phosphoserine1 Publication
    Modified residuei635 – 6351Phosphothreonine1 Publication
    Modified residuei649 – 6491Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP04821.
    PaxDbiP04821.

    Expressioni

    Gene expression databases

    GenevestigatoriP04821.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RAS2P011202EBI-4237,EBI-14838

    Protein-protein interaction databases

    BioGridi31575. 88 interactions.
    DIPiDIP-2261N.
    IntActiP04821. 68 interactions.
    MINTiMINT-364035.
    STRINGi4932.YLR310C.

    Structurei

    3D structure databases

    ProteinModelPortaliP04821.
    SMRiP04821. Positions 61-126, 1104-1543.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1452 – 147322HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini58 – 12871SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini1117 – 1247131N-terminal Ras-GEFPROSITE-ProRule annotationAdd
    BLAST
    Domaini1305 – 1542238Ras-GEFPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
    Contains 1 Ras-GEF domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG265981.
    GeneTreeiENSGT00730000110221.
    HOGENOMiHOG000111493.
    OMAiYATRVMH.
    OrthoDBiEOG78WM2F.

    Family and domain databases

    Gene3Di1.10.840.10. 1 hit.
    InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
    IPR019804. Ras_G-nucl-exch_fac_CS.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    SMARTiSM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48366. SSF48366. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEiPS00720. RASGEF. 1 hit.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04821-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDTNTSIPN TSSAREAGNA SQTPSISSSS NTSTTTNTES SSASLSSSPS     50
    TSELTSIRPI GIVVAAYDFN YPIKKDSSSQ LLSVQQGETI YILNKNSSGW 100
    WDGLVIDDSN GKVNRGWFPQ NFGRPLRDSH LRKHSHPMKK YSSSKSSRRS 150
    SLNSLGNSAY LHVPRNPSKS RRGSSTLSAS LSNAHNAETS SGHNNTVSMN 200
    NSPFSAPNDA SHITPQSSNF NSNASLSQDM TKSADGSSEM NTNAIMNNNE 250
    TNLQTSGEKA GPPLVAEETI KILPLEEIEM IINGIRSNIA STWSPIPLIT 300
    KTSDYKLVYY NKDLDIYCSE LPLISNSIME SDDICDSEPK FPPNDHLVNL 350
    YTRDLRKNAN IEDSSTRSKQ SESEQNRSSL LMEKQDSKET DGNNNSINDD 400
    DNNNENNKNE FNEAGPSSLN SLSAPDLTQN IQSRVVAPSR SSILAKSDIF 450
    YHYSRDIKLW TELQDLTVYY TKTAHKMFLK ENRLNFTKYF DLISDSIVFT 500
    QLGCRLMQHE IKAKSCSKEI KKIFKGLISS LSRISINSHL YFDSAFHRKK 550
    MDTMNDKDND NQENNCSRTE GDDGKIEVDS VHDLVSVPLS GKRNVSTSTT 600
    DTLTPMRSSF STVNENDMEN FSVLGPRNSV NSVVTPRTSI QNSTLEDFSP 650
    SNKNFKSAKS IYEMVDVEFS KFLRHVQLLY FVLQSSVFSD DNTLPQLLPR 700
    FFKGSFSGGS WTNPFSTFIT DEFGNATKNK AVTSNEVTAS SSKNSSISRI 750
    PPKMADAIAS ASGYSANSET NSQIDLKASS AASGSVFTPF NRPSHNRTFS 800
    RARVSKRKKK YPLTVDTLNT MKKKSSQIFE KLNNATGEHL KIISKPKSRI 850
    RNLEINSSTY EQINQNVLLL EILENLDLSI FINLKNLIKT PSILLDLESE 900
    EFLVHAMSSV SSVLTEFFDI KQAFHDIVIR LIMTTQQTTL DDPYLFSSMR 950
    SNFPVGHHEP FKNISNTPLV KGPFHKKNEQ LALSLFHVLV SQDVEFNNLE 1000
    FLNNSDDFKD ACEKYVEISN LACIIVDQLI EERENLLNYA ARMMKNNLTA 1050
    ELLKGEQEKW FDIYSEDYSD DDSENDEAII DDELGSEDYI ERKAANIEKN 1100
    LPWFLTSDYE TSLVYDSRGK IRGGTKEALI EHLTSHELVD AAFNVTMLIT 1150
    FRSILTTREF FYALIYRYNL YPPEGLSYDD YNIWIEKKSN PIKCRVVNIM 1200
    RTFLTQYWTR NYYEPGIPLI LNFAKMVVSE KIPGAEDLLQ KINEKLINEN 1250
    EKEPVDPKQQ DSVSAVVQTT KRDNKSPIHM SSSSLPSSAS SAFFRLKKLK 1300
    LLDIDPYTYA TQLTVLEHDL YLRITMFECL DRAWGTKYCN MGGSPNITKF 1350
    IANANTLTNF VSHTIVKQAD VKTRSKLTQY FVTVAQHCKE LNNFSSMTAI 1400
    VSALYSSPIY RLKKTWDLVS TESKDLLKNL NNLMDSKRNF VKYRELLRSV 1450
    TDVACVPFFG VYLSDLTFTF VGNPDFLHNS TNIINFSKRT KIANIVEEII 1500
    SFKRFHYKLK RLDDIQTVIE ASLENVPHIE KQYQLSLQVE PRSGNTKGST 1550
    HASSASGTKT AKFLSEFTDD KNGNFLKLGK KKPPSRLFR 1589
    Length:1,589
    Mass (Da):179,092
    Last modified:January 1, 1988 - v1
    Checksum:iCA90053435C85465
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti497 – 4971I → Y in CAA27259. (PubMed:3011405)Curated
    Sequence conflicti954 – 96310PVGHHEPFKN → LSVIMNLSR in CAA27259. (PubMed:3011405)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03579 Genomic DNA. Translation: CAA27259.1.
    M15458 Genomic DNA. Translation: AAA34478.1.
    U17247 Genomic DNA. Translation: AAB67360.1.
    U20618 Genomic DNA. Translation: AAB64528.1.
    BK006945 Genomic DNA. Translation: DAA09620.1.
    PIRiA26596. RGBYC5.
    RefSeqiNP_013413.1. NM_001182198.1.

    Genome annotation databases

    EnsemblFungiiYLR310C; YLR310C; YLR310C.
    GeneIDi851019.
    KEGGisce:YLR310C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03579 Genomic DNA. Translation: CAA27259.1 .
    M15458 Genomic DNA. Translation: AAA34478.1 .
    U17247 Genomic DNA. Translation: AAB67360.1 .
    U20618 Genomic DNA. Translation: AAB64528.1 .
    BK006945 Genomic DNA. Translation: DAA09620.1 .
    PIRi A26596. RGBYC5.
    RefSeqi NP_013413.1. NM_001182198.1.

    3D structure databases

    ProteinModelPortali P04821.
    SMRi P04821. Positions 61-126, 1104-1543.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31575. 88 interactions.
    DIPi DIP-2261N.
    IntActi P04821. 68 interactions.
    MINTi MINT-364035.
    STRINGi 4932.YLR310C.

    Proteomic databases

    MaxQBi P04821.
    PaxDbi P04821.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR310C ; YLR310C ; YLR310C .
    GeneIDi 851019.
    KEGGi sce:YLR310C.

    Organism-specific databases

    CYGDi YLR310c.
    SGDi S000004301. CDC25.

    Phylogenomic databases

    eggNOGi NOG265981.
    GeneTreei ENSGT00730000110221.
    HOGENOMi HOG000111493.
    OMAi YATRVMH.
    OrthoDBi EOG78WM2F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32396-MONOMER.
    Reactomei REACT_188984. PPARA activates gene expression.

    Miscellaneous databases

    NextBioi 967589.

    Gene expression databases

    Genevestigatori P04821.

    Family and domain databases

    Gene3Di 1.10.840.10. 1 hit.
    InterProi IPR000651. Ras-like_Gua-exchang_fac_N.
    IPR019804. Ras_G-nucl-exch_fac_CS.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    SMARTi SM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48366. SSF48366. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEi PS00720. RASGEF. 1 hit.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway."
      Broek D., Toda T., Michaeli T., Levin L., Birchmeier C., Zoller M., Powers S., Wigler M.
      Cell 48:789-799(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Characterization, cloning and sequence analysis of the CDC25 gene which controls the cyclic AMP level of Saccharomyces cerevisiae."
      Camonis J.H., Kalekine M., Gondre B., Garreau H., Boy-Marcotte E., Jacquet M.
      EMBO J. 5:375-380(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The CDC25 'Start' gene of Saccharomyces cerevisiae: sequencing of the active C-terminal fragment and regional homologies with rhodopsin and cytochrome P450."
      Daniel J.H.
      Curr. Genet. 10:879-885(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 877-1589.
    6. "Domains of the Saccharomyces cerevisiae CDC25 gene controlling mitosis and meiosis."
      Munder T., Mink M., Kuentzel H.
      Mol. Gen. Genet. 214:271-277(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAINS.
    7. "The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras."
      Jones S., Vignais M.L., Broach J.R.
      Mol. Cell. Biol. 11:2641-2646(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151; SER-154; SER-580; SER-596; SER-632; THR-635 AND SER-649, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCDC25_YEAST
    AccessioniPrimary (citable) accession number: P04821
    Secondary accession number(s): D6VYV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 1, 1988
    Last modified: October 1, 2014
    This is version 149 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 319 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3