P04817 (CAN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine permease

Gene names

Name:	CAN1
Ordered Locus Names:	YEL063C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High-affinity permease for arginine. Ref.5
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family. [View classification]

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD plasma membrane Inferred from direct assay. Source: SGD
Molecular function	arginine transmembrane transporter activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Arginine permease		PRO_0000054148
Regions
	Transmembrane	93 – 110	18	Helical; Potential
	Transmembrane	230 – 254	25	Helical; Potential
	Transmembrane	324 – 343	20	Helical; Potential
	Transmembrane	377 – 396	20	Helical; Potential
	Transmembrane	420 – 437	18	Helical; Potential
	Transmembrane	451 – 469	19	Helical; Potential
	Transmembrane	494 – 518	25	Helical; Potential
	Transmembrane	525 – 548	24	Helical; Potential
	Region	1 – 92	92	Hydrophilic
	Region	549 – 590	42	Hydrophilic
Amino acid modifications
	Modified residue	38	1	Phosphoserine Ref.6 Ref.7
Experimental info
	Sequence conflict	534	1	I → V in CAA27416. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P04817 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 4E5A21C77145330D	FASTA	590	65,785
	10 20 30 40 50 60 MTNSKEDADI EEKHMYNEPV TTLFHDVEAS QTHHRRGSIP LKDEKSKELY PLRSFPTRVN 70 80 90 100 110 120 GEDTFSMEDG IGDEDEGEVQ NAEVKRELKQ RHIGMIALGG TIGTGLFIGL STPLTNAGPV 130 140 150 160 170 180 GALISYLFMG SLAYSVTQSL GEMATFIPVT SSFTVFSQRF LSPAFGAANG YMYWFSWAIT 190 200 210 220 230 240 FALELSVVGQ VIQFWTYKVP LAAWISIFWV IITIMNLFPV KYYGEFEFWV ASIKVLAIIG 250 260 270 280 290 300 FLIYCFCMVC GAGVTGPVGF RYWRNPGAWG PGIISKDKNE GRFLGWVSSL INAAFTFQGT 310 320 330 340 350 360 ELVGITAGEA ANPRKSVPRA IKKVVFRILT FYIGSLLFIG LLVPYNDPKL TQSTSYVSTS 370 380 390 400 410 420 PFIIAIENSG TKVLPHIFNA VILTTIISAA NSNIYVGSRI LFGLSKNKLA PKFLSRTTKG 430 440 450 460 470 480 GVPYIAVFVT AAFGALAYME TSTGGDKVFE WLLNITGVAG FFAWLFISIS HIRFMQALKY 490 500 510 520 530 540 RGISRDELPF KAKLMPGLAY YAATFMTIII IIQGFTAFAP KFNGVSFAAA YISIFLFLAV 550 560 570 580 590 WILFQCIFRC RFIWKIGDVD IDSDRRDIEA IVWEDHEPKT FWDKFWNVVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast arginine permease: nucleotide sequence of the CAN1 gene." Ahmad M., Bussey H. Curr. Genet. 10:587-592(1986) [PubMed: 3327612] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular characterization of the CAN1 locus in Saccharomyces cerevisiae. A transmembrane protein without N-terminal hydrophobic signal sequence." Hoffmann W. J. Biol. Chem. 260:11831-11837(1985) [PubMed: 3900064] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae." Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S. Curr. Genet. 36:317-328(1999) [PubMed: 10654085] [Abstract] Cited for: FUNCTION.
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03784 Genomic DNA. Translation: CAA27416.1. M11724 Genomic DNA. Translation: AAA34467.1. U18795 Genomic DNA. Translation: AAB65024.1. BK006939 Genomic DNA. Translation: DAA07591.1.
PIR	QRBYPR. A23922.
RefSeq	NP_010851.1. NM_001178878.1.
3D structure databases
ProteinModelPortal	P04817.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4062N.
IntAct	P04817. 71 interactions.
MINT	MINT-476477.
STRING	P04817.
Protein family/group databases
TCDB	2.A.3.10.4. amino acid-polyamine-organocation (APC) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL063C; YEL063C; YEL063C.
GeneID	856646.
KEGG	sce:YEL063C.
NMPDR	fig|4932.3.peg.1899.
Organism-specific databases
CYGD	YEL063c.
SGD	S000000789. CAN1.
Phylogenomic databases
eggNOG	fuNOG04851.
GeneTree	EFGT00050000001131.
HOGENOM	HBG492579.
OMA	GAANGYM.
OrthoDB	EOG4JDMG2.
Gene expression databases
ArrayExpress	P04817.
Genevestigator	P04817.
GermOnline	YEL063C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004841. AA-permease_dom. IPR002293. AA/rel_permease1. IPR004762. Amino_acid_permease_fungi. IPR004840. Amoino_acid_permease_CS. [Graphical view]
KO	K03293.
PANTHER	PTHR11785. AA/rel_permease1. 1 hit.
Pfam	PF00324. AA_permease. 1 hit. [Graphical view]
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00913. 2A0310. 1 hit.
PROSITE	PS00218. AMINO_ACID_PERMEASE_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982616.

Entry information

Entry name

CAN1_YEAST

Accession

Primary (citable) accession number: P04817
Secondary accession number(s): D3DLI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program