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Protein

Arginine permease CAN1

Gene

CAN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

High-affinity permease for arginine (PubMed:8436127, PubMed:9231419, PubMed:10654085, PubMed:11746604).4 Publications

GO - Molecular functioni

  • arginine transmembrane transporter activity Source: SGD
  • basic amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • arginine transport Source: SGD
  • basic amino acid transport Source: SGD
  • transmembrane transport Source: SGD

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30178-MONOMER

Protein family/group databases

TCDBi2.A.3.10.4 the amino acid-polyamine-organocation (apc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine permease CAN1
Alternative name(s):
Canavanine resistance protein 11 Publication
Gene namesi
Name:CAN11 Publication
Ordered Locus Names:YEL063C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL063C
SGDiS000000789 CAN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21
Transmembranei235 – 255HelicalSequence analysisAdd BLAST21
Transmembranei283 – 303HelicalSequence analysisAdd BLAST21
Transmembranei324 – 344HelicalSequence analysisAdd BLAST21
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Transmembranei420 – 440HelicalSequence analysisAdd BLAST21
Transmembranei449 – 469HelicalSequence analysisAdd BLAST21
Transmembranei498 – 518HelicalSequence analysisAdd BLAST21
Transmembranei524 – 544HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Abolishes canavanine sensitivity (PubMed:3327612). Extends replicative lifespan and leads to distinct changes in transcriptional and translational profiles, including translational activation of the GCN4 transcription factor (PubMed:28228255).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi113P → L in CAN1-343; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi148P → L in CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine. 1 Publication1
Mutagenesisi149V → F in CAN1-315; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi152S → F in CAN1-342; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi173Y → D in CAN1-306; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi173Y → H in CAN1-327; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi308G → A in CAN1-341; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi313P → S in CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine. 1 Publication1
Mutagenesisi354 – 355Missing in CAN1-318; confers citrulline transport activity in GAP1-deleted cells. 1 Publication2
Mutagenesisi356Y → H in CAN1-340; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi356Y → N in CAN1-339; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi451W → C in CAN1-328; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi451W → L in CAN1-316; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi451W → S in CAN1-335; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi461F → S in CAN1-307; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541481 – 590Arginine permease CAN1Add BLAST590

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54PhosphoserineBy similarity1
Modified residuei66PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated probably at multiple sites (PubMed:9544242).1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP04817
PRIDEiP04817

PTM databases

iPTMnetiP04817

Interactioni

Subunit structurei

Interacts with RRT2 (PubMed:21880895).1 Publication

Protein-protein interaction databases

BioGridi3666672 interactors.
DIPiDIP-4062N
IntActiP04817 45 interactors.
MINTiP04817
STRINGi4932.YEL063C

Structurei

3D structure databases

ProteinModelPortaliP04817
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744
HOGENOMiHOG000261850
InParanoidiP04817
KOiK16261
OMAiINFITRY
OrthoDBiEOG092C164R

Family and domain databases

InterProiView protein in InterPro
IPR004841 AA-permease/SLC12A_dom
IPR002293 AA/rel_permease1
IPR004762 Amino_acid_permease_fungi
IPR004840 Amoino_acid_permease_CS
PfamiView protein in Pfam
PF00324 AA_permease, 1 hit
PIRSFiPIRSF006060 AA_transporter, 1 hit
TIGRFAMsiTIGR00913 2A0310, 1 hit
PROSITEiView protein in PROSITE
PS00218 AMINO_ACID_PERMEASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P04817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNSKEDADI EEKHMYNEPV TTLFHDVEAS QTHHRRGSIP LKDEKSKELY
60 70 80 90 100
PLRSFPTRVN GEDTFSMEDG IGDEDEGEVQ NAEVKRELKQ RHIGMIALGG
110 120 130 140 150
TIGTGLFIGL STPLTNAGPV GALISYLFMG SLAYSVTQSL GEMATFIPVT
160 170 180 190 200
SSFTVFSQRF LSPAFGAANG YMYWFSWAIT FALELSVVGQ VIQFWTYKVP
210 220 230 240 250
LAAWISIFWV IITIMNLFPV KYYGEFEFWV ASIKVLAIIG FLIYCFCMVC
260 270 280 290 300
GAGVTGPVGF RYWRNPGAWG PGIISKDKNE GRFLGWVSSL INAAFTFQGT
310 320 330 340 350
ELVGITAGEA ANPRKSVPRA IKKVVFRILT FYIGSLLFIG LLVPYNDPKL
360 370 380 390 400
TQSTSYVSTS PFIIAIENSG TKVLPHIFNA VILTTIISAA NSNIYVGSRI
410 420 430 440 450
LFGLSKNKLA PKFLSRTTKG GVPYIAVFVT AAFGALAYME TSTGGDKVFE
460 470 480 490 500
WLLNITGVAG FFAWLFISIS HIRFMQALKY RGISRDELPF KAKLMPGLAY
510 520 530 540 550
YAATFMTIII IIQGFTAFAP KFNGVSFAAA YISIFLFLAV WILFQCIFRC
560 570 580 590
RFIWKIGDVD IDSDRRDIEA IVWEDHEPKT FWDKFWNVVA
Length:590
Mass (Da):65,785
Last modified:February 1, 1995 - v2
Checksum:i4E5A21C77145330D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti534I → V in CAA27416 (PubMed:3327612).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03784 Genomic DNA Translation: CAA27416.1
M11724 Genomic DNA Translation: AAA34467.1
U18795 Genomic DNA Translation: AAB65024.1
BK006939 Genomic DNA Translation: DAA07591.1
PIRiA23922 QRBYPR
RefSeqiNP_010851.1, NM_001178878.1

Genome annotation databases

EnsemblFungiiYEL063C; YEL063C; YEL063C
GeneIDi856646
KEGGisce:YEL063C

Similar proteinsi

Entry informationi

Entry nameiCAN1_YEAST
AccessioniPrimary (citable) accession number: P04817
Secondary accession number(s): D3DLI7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome