Arginine permease - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P04817 (CAN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 122. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Arginine permease

Gene names

Name:	CAN1
Ordered Locus Names:	YEL063C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High-affinity permease for arginine. Ref.5
Subunit structure	Interacts with RRT2. Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Note: Recycled via the retromer-mediated pathway. Ref.8
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family. [View classification]

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell membrane Endosome Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD membrane raft Inferred from direct assay PubMed 15536122. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278 PubMed 16823961. Source: SGD plasma membrane Inferred from direct assay PubMed 15536122. Source: SGD
Molecular_function	arginine transmembrane transporter activity Inferred from mutant phenotype PubMed 10753927. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 590

590

Arginine permease

PRO_0000054148

Regions

Transmembrane

93 – 110

Helical; Potential

Transmembrane

230 – 254

Helical; Potential

Transmembrane

324 – 343

Helical; Potential

Transmembrane

377 – 396

Helical; Potential

Transmembrane

420 – 437

Helical; Potential

Transmembrane

451 – 469

Helical; Potential

Transmembrane

494 – 518

Helical; Potential

Transmembrane

525 – 548

Helical; Potential

Region

1 – 92

Hydrophilic

Region

549 – 590

Hydrophilic

Amino acid modifications

Modified residue

Phosphoserine Ref.6 Ref.7

Experimental info

Sequence conflict

534

I → V in CAA27416. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P04817 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 4E5A21C77145330D
Show »

FASTA

590

65,785

        10         20         30         40         50         60 
MTNSKEDADI EEKHMYNEPV TTLFHDVEAS QTHHRRGSIP LKDEKSKELY PLRSFPTRVN 

        70         80         90        100        110        120 
GEDTFSMEDG IGDEDEGEVQ NAEVKRELKQ RHIGMIALGG TIGTGLFIGL STPLTNAGPV 

       130        140        150        160        170        180 
GALISYLFMG SLAYSVTQSL GEMATFIPVT SSFTVFSQRF LSPAFGAANG YMYWFSWAIT 

       190        200        210        220        230        240 
FALELSVVGQ VIQFWTYKVP LAAWISIFWV IITIMNLFPV KYYGEFEFWV ASIKVLAIIG 

       250        260        270        280        290        300 
FLIYCFCMVC GAGVTGPVGF RYWRNPGAWG PGIISKDKNE GRFLGWVSSL INAAFTFQGT 

       310        320        330        340        350        360 
ELVGITAGEA ANPRKSVPRA IKKVVFRILT FYIGSLLFIG LLVPYNDPKL TQSTSYVSTS 

       370        380        390        400        410        420 
PFIIAIENSG TKVLPHIFNA VILTTIISAA NSNIYVGSRI LFGLSKNKLA PKFLSRTTKG 

       430        440        450        460        470        480 
GVPYIAVFVT AAFGALAYME TSTGGDKVFE WLLNITGVAG FFAWLFISIS HIRFMQALKY 

       490        500        510        520        530        540 
RGISRDELPF KAKLMPGLAY YAATFMTIII IIQGFTAFAP KFNGVSFAAA YISIFLFLAV 

       550        560        570        580        590 
WILFQCIFRC RFIWKIGDVD IDSDRRDIEA IVWEDHEPKT FWDKFWNVVA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast arginine permease: nucleotide sequence of the CAN1 gene." Ahmad M., Bussey H. Curr. Genet. 10:587-592(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular characterization of the CAN1 locus in Saccharomyces cerevisiae. A transmembrane protein without N-terminal hydrophobic signal sequence." Hoffmann W. J. Biol. Chem. 260:11831-11837(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae." Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S. Curr. Genet. 36:317-328(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38, MASS SPECTROMETRY.
[8]	"Two novel WD40 domain-containing proteins, Ere1 and Ere2, function in the retromer-mediated endosomal recycling pathway." Shi Y., Stefan C.J., Rue S.M., Teis D., Emr S.D. Mol. Biol. Cell 22:4093-4107(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, RECYCLING, INTERACTION WITH RRT2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03784 Genomic DNA. Translation: CAA27416.1. M11724 Genomic DNA. Translation: AAA34467.1. U18795 Genomic DNA. Translation: AAB65024.1. BK006939 Genomic DNA. Translation: DAA07591.1.
PIR	QRBYPR. A23922.
RefSeq	NP_010851.1. NM_001178878.1.
3D structure databases
ProteinModelPortal	P04817.
SMR	P04817. Positions 87-471.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4062N.
IntAct	P04817. 1 interaction.
MINT	MINT-476477.
STRING	4932.YEL063C.
Protein family/group databases
TCDB	2.A.3.10.4. amino acid-polyamine-organocation (APC) family.
Proteomic databases
PaxDb	P04817.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL063C; YEL063C; YEL063C.
GeneID	856646.
KEGG	sce:YEL063C.
Organism-specific databases
CYGD	YEL063c.
SGD	S000000789. CAN1.
Phylogenomic databases
eggNOG	COG0833.
GeneTree	ENSGT00510000049744.
HOGENOM	HOG000261850.
KO	K16261.
OMA	HNFENEG.
OrthoDB	EOG4JDMG2.
Gene expression databases
Genevestigator	P04817.
GermOnline	YEL063C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004841. AA-permease_dom. IPR002293. AA/rel_permease1. IPR004762. Amino_acid_permease_fungi. IPR004840. Amoino_acid_permease_CS. [Graphical view]
PANTHER	PTHR11785. PTHR11785. 1 hit.
Pfam	PF00324. AA_permease. 1 hit. [Graphical view]
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00913. 2A0310. 1 hit.
PROSITE	PS00218. AMINO_ACID_PERMEASE_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982616.

Entry information

Entry name

CAN1_YEAST

Accession

Primary (citable) accession number: P04817
Secondary accession number(s): D3DLI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents