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Protein

Arginine permease CAN1

Gene

CAN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity permease for arginine (PubMed:8436127, PubMed:9231419, PubMed:10654085, PubMed:11746604).4 Publications

GO - Molecular functioni

  • arginine transmembrane transporter activity Source: SGD
  • basic amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • arginine transport Source: SGD
  • basic amino acid transport Source: SGD
  • transmembrane transport Source: SGD

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30178-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.4. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine permease CAN1
Alternative name(s):
Canavanine resistance protein 11 Publication
Gene namesi
Name:CAN11 Publication
Ordered Locus Names:YEL063C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL063C.
SGDiS000000789. CAN1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21
Transmembranei235 – 255HelicalSequence analysisAdd BLAST21
Transmembranei283 – 303HelicalSequence analysisAdd BLAST21
Transmembranei324 – 344HelicalSequence analysisAdd BLAST21
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Transmembranei420 – 440HelicalSequence analysisAdd BLAST21
Transmembranei449 – 469HelicalSequence analysisAdd BLAST21
Transmembranei498 – 518HelicalSequence analysisAdd BLAST21
Transmembranei524 – 544HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endosome membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane raft Source: SGD
  • plasma membrane Source: SGD

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Abolishes canavanine sensitivity (PubMed:3327612). Extends replicative lifespan and leads to distinct changes in transcriptional and translational profiles, including translational activation of the GCN4 transcription factor (PubMed:28228255).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi113P → L in CAN1-343; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi148P → L in CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine. 1 Publication1
Mutagenesisi149V → F in CAN1-315; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi152S → F in CAN1-342; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi173Y → D in CAN1-306; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi173Y → H in CAN1-327; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi308G → A in CAN1-341; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi313P → S in CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine. 1 Publication1
Mutagenesisi354 – 355Missing in CAN1-318; confers citrulline transport activity in GAP1-deleted cells. 1 Publication2
Mutagenesisi356Y → H in CAN1-340; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi356Y → N in CAN1-339; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi451W → C in CAN1-328; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi451W → L in CAN1-316; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi451W → S in CAN1-335; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1
Mutagenesisi461F → S in CAN1-307; confers citrulline transport activity in GAP1-deleted cells. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541481 – 590Arginine permease CAN1Add BLAST590

Post-translational modificationi

Phosphorylated probably at multiple sites (PubMed:9544242).1 Publication

Proteomic databases

PRIDEiP04817.

PTM databases

iPTMnetiP04817.

Interactioni

Subunit structurei

Interacts with RRT2 (PubMed:21880895).1 Publication

Protein-protein interaction databases

BioGridi36666. 70 interactors.
DIPiDIP-4062N.
IntActiP04817. 45 interactors.
MINTiMINT-476477.
STRINGi4932.YEL063C.

Structurei

3D structure databases

ProteinModelPortaliP04817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261850.
InParanoidiP04817.
KOiK16261.
OMAiKRRWLNS.
OrthoDBiEOG092C164R.

Family and domain databases

InterProiView protein in InterPro
IPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
PfamiView protein in Pfam
PF00324. AA_permease. 1 hit.
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiView protein in PROSITE
PS00218. AMINO_ACID_PERMEASE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P04817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNSKEDADI EEKHMYNEPV TTLFHDVEAS QTHHRRGSIP LKDEKSKELY
60 70 80 90 100
PLRSFPTRVN GEDTFSMEDG IGDEDEGEVQ NAEVKRELKQ RHIGMIALGG
110 120 130 140 150
TIGTGLFIGL STPLTNAGPV GALISYLFMG SLAYSVTQSL GEMATFIPVT
160 170 180 190 200
SSFTVFSQRF LSPAFGAANG YMYWFSWAIT FALELSVVGQ VIQFWTYKVP
210 220 230 240 250
LAAWISIFWV IITIMNLFPV KYYGEFEFWV ASIKVLAIIG FLIYCFCMVC
260 270 280 290 300
GAGVTGPVGF RYWRNPGAWG PGIISKDKNE GRFLGWVSSL INAAFTFQGT
310 320 330 340 350
ELVGITAGEA ANPRKSVPRA IKKVVFRILT FYIGSLLFIG LLVPYNDPKL
360 370 380 390 400
TQSTSYVSTS PFIIAIENSG TKVLPHIFNA VILTTIISAA NSNIYVGSRI
410 420 430 440 450
LFGLSKNKLA PKFLSRTTKG GVPYIAVFVT AAFGALAYME TSTGGDKVFE
460 470 480 490 500
WLLNITGVAG FFAWLFISIS HIRFMQALKY RGISRDELPF KAKLMPGLAY
510 520 530 540 550
YAATFMTIII IIQGFTAFAP KFNGVSFAAA YISIFLFLAV WILFQCIFRC
560 570 580 590
RFIWKIGDVD IDSDRRDIEA IVWEDHEPKT FWDKFWNVVA
Length:590
Mass (Da):65,785
Last modified:February 1, 1995 - v2
Checksum:i4E5A21C77145330D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti534I → V in CAA27416 (PubMed:3327612).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03784 Genomic DNA. Translation: CAA27416.1.
M11724 Genomic DNA. Translation: AAA34467.1.
U18795 Genomic DNA. Translation: AAB65024.1.
BK006939 Genomic DNA. Translation: DAA07591.1.
PIRiA23922. QRBYPR.
RefSeqiNP_010851.1. NM_001178878.1.

Genome annotation databases

EnsemblFungiiYEL063C; YEL063C; YEL063C.
GeneIDi856646.
KEGGisce:YEL063C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCAN1_YEAST
AccessioniPrimary (citable) accession number: P04817
Secondary accession number(s): D3DLI7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 1, 1995
Last modified: July 5, 2017
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names