P04816 (LIVK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine-specific-binding protein
Short name=L-BP
Short name=LS-BP

Gene names

Name:	livK
Ordered Locus Names:	b3458, JW3423

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the leucine-binding protein family.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Periplasm
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Ref.8

Chain

24 – 369

346

Leucine-specific-binding protein

PRO_0000017702

Amino acid modifications

Disulfide bond

76 ↔ 101

By similarity

Experimental info

Sequence conflict

3

1

R → A in CAA23579. Ref.7

Sequence conflict

43

1

D → I Ref.1

Sequence conflict

43

1

D → I Ref.2

Sequence conflict

43

1

D → I Ref.3

Sequence conflict

50

1

R → E Ref.1

Sequence conflict

50

1

R → E Ref.2

Sequence conflict

50

1

R → E Ref.3

Sequence conflict

126

1

N → A Ref.1

Sequence conflict

126

1

N → A Ref.2

Sequence conflict

126

1

N → A Ref.3

Secondary structure

1

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369

Helix Strand Turn

Details...

Beta strand

26 – 32

7

Beta strand

34 – 36

3

Helix

39 – 58

20

Turn

59 – 61

3

Beta strand

67 – 73

7

Helix

78 – 90

13

Beta strand

95 – 97

3

Helix

102 – 115

14

Beta strand

118 – 121

4

Helix

127 – 130

4

Beta strand

135 – 139

5

Helix

144 – 146

3

Helix

147 – 157

11

Beta strand

162 – 168

7

Helix

172 – 187

16

Beta strand

192 – 197

6

Helix

206 – 214

9

Beta strand

219 – 224

6

Helix

226 – 238

13

Beta strand

244 – 247

4

Helix

249 – 251

3

Helix

256 – 260

5

Helix

261 – 264

4

Beta strand

268 – 271

4

Helix

275 – 277

3

Helix

279 – 281

3

Helix

282 – 290

9

Helix

298 – 317

20

Helix

322 – 332

11

Beta strand

334 – 336

3

Beta strand

339 – 341

3

Beta strand

347 – 351

5

Beta strand

355 – 359

5

Beta strand

365 – 367

3

Sequences

	Sequence		Length	Mass (Da)
	P04816 [UniParc]. Last modified July 19, 2003. Version 2. Checksum: 42B939A18D8723B2	FASTA	369	39,379
	10 20 30 40 50 60 MKRNAKTIIA GMIALAISHT AMADDIKVAV VGAMSGPIAQ WGDMEFNGAR QAIKDINAKG 70 80 90 100 110 120 GIKGDKLVGV EYDDACDPKQ AVAVANKIVN DGIKYVIGHL CSSSTQPASD IYEDEGILMI 130 140 150 160 170 180 SPGATNPELT QRGYQHIMRT AGLDSSQGPT AAKYILETVK PQRIAIIHDK QQYGEGLARS 190 200 210 220 230 240 VQDGLKAANA NVVFFDGITA GEKDFSALIA RLKKENIDFV YYGGYYPEMG QMLRQARSVG 250 260 270 280 290 300 LKTQFMGPEG VGNASLSNIA GDAAEGMLVT MPKRYDQDPA NQGIVDALKA DKKDPSGPYV 310 320 330 340 350 360 WITYAAVQSL ATALERTGSD EPLALVKDLK ANGANTVIGP LNWDEKGDLK GFDFGVFQWH ADGSSTAAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete nucleotide sequences of the Escherichia coli LIV-BP and LS-BP genes. Implications for the mechanism of high-affinity branched-chain amino acid transport." Landick R., Oxender D.L. J. Biol. Chem. 260:8257-8261(1985) [PubMed: 3891753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The leucine binding proteins of Escherichia coli as models for studying the relationships between protein structure and function." Antonucci T.K., Landick R., Oxender D.L. J. Cell. Biochem. 29:209-216(1985) [PubMed: 4077929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli." Adams M.D., Wagner L.M., Graddis T.J., Landick R., Antonucci T.K., Gibson A.L., Oxender D.L. J. Biol. Chem. 265:11436-11443(1990) [PubMed: 2195019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Amino-terminal sequence and processing of the precursor of the leucine-specific binding protein, and evidence for conformational differences between the precursor and the mature form." Oxender D.L., Anderson J.J., Daniels C.J., Landick R., Gunsalus R.P., Zurawski G., Yanofsky C. Proc. Natl. Acad. Sci. U.S.A. 77:2005-2009(1980) [PubMed: 6990419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36.
[8]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-35. Strain: K12 / EMG2.
[9]	"Structure of the L-leucine-binding protein refined at 2.4-A resolution and comparison with the Leu/Ile/Val-binding protein structure." Sack J.S., Trakhanov S.D., Tsigannik I.H., Quiocho F.A. J. Mol. Biol. 206:193-207(1989) [PubMed: 2649683] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M29378 Genomic DNA. Translation: AAA24090.1.
J05516 Genomic DNA. Translation: AAA83883.1.
U00039 Genomic DNA. Translation: AAB18433.1.
U00096 Genomic DNA. Translation: AAC76483.1.
AP009048 Genomic DNA. Translation: BAE77835.1.
V00301 Genomic DNA. Translation: CAA23579.1.

PIR

BLECL. E65142.

RefSeq

NP_417915.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1USG	X-ray	1.53	A	24-369	[»]
1USI	X-ray	1.80	A/C	24-369	[»]
1USK	X-ray	2.00	A/B/C/D	24-369	[»]
2LBP	X-ray	2.40	A	24-369	[»]

ProteinModelPortal

P04816.

SMR

P04816. Positions 24-368.

ModBase

Search...

Protein-protein interaction databases

IntAct

P04816. 3 interactions.

Protein family/group databases

TCDB

3.A.1.4.1. ATP-binding cassette (ABC) superfamily.

2D gel databases

SWISS-2DPAGE

P04816.

2DBase-Ecoli

P04816.

ECO2DBASE

B040.8. 6TH EDITION.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000003254; EBESCP00000003254; EBESCG00000002670.
EBESCT00000003255; EBESCP00000003255; EBESCG00000002670.
EBESCT00000014562; EBESCP00000013853; EBESCG00000013623.

GeneID

947964.

GenomeReviews

Gene locus JW3423 in contig AP009048_GR.
Gene locus b3458 in contig U00096_GR.

KEGG

ecj:JW3423.
eco:b3458.

PATRIC

32122358. VBIEscCol129921_3556.

Organism-specific databases

EchoBASE

EB0535.

EcoGene

EG10540. livK.

Phylogenomic databases

eggNOG

COG0683.

GeneTree

EBGT00050000010005.

HOGENOM

HBG695960.

OMA

MEFTGAR.

PhylomeDB

P04816.

ProtClustDB

PRK15404.

Enzyme and pathway databases

BioCyc

EcoCyc:LIVK-MONOMER.

Gene expression databases

Genevestigator

P04816.

Family and domain databases

InterPro

IPR001828. ANF_lig-bd_rcpt.
IPR000709. Leu_Ile_Val-bd.
[Graphical view]

KO

K01999.

Pfam

PF01094. ANF_receptor. 1 hit.
[Graphical view]

PRINTS

PR00337. LEUILEVALBP.

ProtoNet

Search...

Entry information

Entry name

LIVK_ECOLI

Accession

Primary (citable) accession number: P04816
Secondary accession number(s): Q2M7C1, Q47343

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	July 19, 2003
Last modified:	January 25, 2012
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program