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Protein

Leucine-specific-binding protein

Gene

livK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli.

GO - Molecular functioni

  • L-leucine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • leucine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:LIVK-MONOMER.
ECOL316407:JW3423-MONOMER.
MetaCyc:LIVK-MONOMER.

Protein family/group databases

TCDBi3.A.1.4.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-specific-binding protein
Short name:
L-BP
Short name:
LS-BP
Gene namesi
Name:livK
Ordered Locus Names:b3458, JW3423
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10540. livK.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000001770224 – 369Leucine-specific-binding proteinAdd BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi76 ↔ 101By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP04816.
PRIDEiP04816.

2D gel databases

SWISS-2DPAGEP04816.

Interactioni

Protein-protein interaction databases

BioGridi4262495. 12 interactors.
IntActiP04816. 3 interactors.
STRINGi511145.b3458.

Structurei

Secondary structure

1369
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 32Combined sources7
Beta strandi34 – 36Combined sources3
Helixi39 – 58Combined sources20
Turni59 – 61Combined sources3
Beta strandi67 – 73Combined sources7
Helixi78 – 90Combined sources13
Beta strandi95 – 97Combined sources3
Helixi102 – 115Combined sources14
Beta strandi118 – 121Combined sources4
Helixi127 – 130Combined sources4
Beta strandi135 – 139Combined sources5
Helixi144 – 146Combined sources3
Helixi147 – 157Combined sources11
Beta strandi162 – 168Combined sources7
Helixi172 – 187Combined sources16
Beta strandi192 – 197Combined sources6
Helixi206 – 214Combined sources9
Beta strandi219 – 224Combined sources6
Helixi226 – 238Combined sources13
Beta strandi244 – 247Combined sources4
Helixi249 – 251Combined sources3
Helixi256 – 260Combined sources5
Helixi261 – 264Combined sources4
Beta strandi268 – 271Combined sources4
Helixi275 – 277Combined sources3
Helixi279 – 281Combined sources3
Helixi282 – 290Combined sources9
Helixi298 – 317Combined sources20
Helixi322 – 332Combined sources11
Beta strandi334 – 336Combined sources3
Beta strandi339 – 341Combined sources3
Beta strandi347 – 351Combined sources5
Beta strandi355 – 359Combined sources5
Beta strandi365 – 367Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1USGX-ray1.53A24-369[»]
1USIX-ray1.80A/C24-369[»]
1USKX-ray2.00A/B/C/D24-369[»]
2LBPX-ray2.40A24-369[»]
ProteinModelPortaliP04816.
SMRiP04816.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04816.

Family & Domainsi

Sequence similaritiesi

Belongs to the leucine-binding protein family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CP9. Bacteria.
COG0683. LUCA.
HOGENOMiHOG000130989.
InParanoidiP04816.
KOiK01999.
OMAiMITPGAT.
PhylomeDBiP04816.

Family and domain databases

InterProiIPR028081. Leu-bd.
IPR000709. Leu_Ile_Val-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR30483:SF16. PTHR30483:SF16. 1 hit.
PfamiPF13458. Peripla_BP_6. 1 hit.
[Graphical view]
PRINTSiPR00337. LEUILEVALBP.
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRNAKTIIA GMIALAISHT AMADDIKVAV VGAMSGPIAQ WGDMEFNGAR
60 70 80 90 100
QAIKDINAKG GIKGDKLVGV EYDDACDPKQ AVAVANKIVN DGIKYVIGHL
110 120 130 140 150
CSSSTQPASD IYEDEGILMI SPGATNPELT QRGYQHIMRT AGLDSSQGPT
160 170 180 190 200
AAKYILETVK PQRIAIIHDK QQYGEGLARS VQDGLKAANA NVVFFDGITA
210 220 230 240 250
GEKDFSALIA RLKKENIDFV YYGGYYPEMG QMLRQARSVG LKTQFMGPEG
260 270 280 290 300
VGNASLSNIA GDAAEGMLVT MPKRYDQDPA NQGIVDALKA DKKDPSGPYV
310 320 330 340 350
WITYAAVQSL ATALERTGSD EPLALVKDLK ANGANTVIGP LNWDEKGDLK
360
GFDFGVFQWH ADGSSTAAK
Length:369
Mass (Da):39,379
Last modified:July 19, 2003 - v2
Checksum:i42B939A18D8723B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3R → A in CAA23579 (PubMed:6990419).Curated1
Sequence conflicti43D → I (PubMed:3891753).Curated1
Sequence conflicti43D → I (PubMed:4077929).Curated1
Sequence conflicti43D → I (PubMed:2195019).Curated1
Sequence conflicti50R → E (PubMed:3891753).Curated1
Sequence conflicti50R → E (PubMed:4077929).Curated1
Sequence conflicti50R → E (PubMed:2195019).Curated1
Sequence conflicti126N → A (PubMed:3891753).Curated1
Sequence conflicti126N → A (PubMed:4077929).Curated1
Sequence conflicti126N → A (PubMed:2195019).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29378 Genomic DNA. Translation: AAA24090.1.
J05516 Genomic DNA. Translation: AAA83883.1.
U00039 Genomic DNA. Translation: AAB18433.1.
U00096 Genomic DNA. Translation: AAC76483.1.
AP009048 Genomic DNA. Translation: BAE77835.1.
V00301 Genomic DNA. Translation: CAA23579.1.
PIRiE65142. BLECL.
RefSeqiNP_417915.1. NC_000913.3.
WP_000827696.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76483; AAC76483; b3458.
BAE77835; BAE77835; BAE77835.
GeneIDi947964.
KEGGiecj:JW3423.
eco:b3458.
PATRICi32122358. VBIEscCol129921_3556.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29378 Genomic DNA. Translation: AAA24090.1.
J05516 Genomic DNA. Translation: AAA83883.1.
U00039 Genomic DNA. Translation: AAB18433.1.
U00096 Genomic DNA. Translation: AAC76483.1.
AP009048 Genomic DNA. Translation: BAE77835.1.
V00301 Genomic DNA. Translation: CAA23579.1.
PIRiE65142. BLECL.
RefSeqiNP_417915.1. NC_000913.3.
WP_000827696.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1USGX-ray1.53A24-369[»]
1USIX-ray1.80A/C24-369[»]
1USKX-ray2.00A/B/C/D24-369[»]
2LBPX-ray2.40A24-369[»]
ProteinModelPortaliP04816.
SMRiP04816.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262495. 12 interactors.
IntActiP04816. 3 interactors.
STRINGi511145.b3458.

Protein family/group databases

TCDBi3.A.1.4.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP04816.

Proteomic databases

PaxDbiP04816.
PRIDEiP04816.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76483; AAC76483; b3458.
BAE77835; BAE77835; BAE77835.
GeneIDi947964.
KEGGiecj:JW3423.
eco:b3458.
PATRICi32122358. VBIEscCol129921_3556.

Organism-specific databases

EchoBASEiEB0535.
EcoGeneiEG10540. livK.

Phylogenomic databases

eggNOGiENOG4105CP9. Bacteria.
COG0683. LUCA.
HOGENOMiHOG000130989.
InParanoidiP04816.
KOiK01999.
OMAiMITPGAT.
PhylomeDBiP04816.

Enzyme and pathway databases

BioCyciEcoCyc:LIVK-MONOMER.
ECOL316407:JW3423-MONOMER.
MetaCyc:LIVK-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP04816.
PROiP04816.

Family and domain databases

InterProiIPR028081. Leu-bd.
IPR000709. Leu_Ile_Val-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PANTHERiPTHR30483:SF16. PTHR30483:SF16. 1 hit.
PfamiPF13458. Peripla_BP_6. 1 hit.
[Graphical view]
PRINTSiPR00337. LEUILEVALBP.
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLIVK_ECOLI
AccessioniPrimary (citable) accession number: P04816
Secondary accession number(s): Q2M7C1, Q47343
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 19, 2003
Last modified: November 2, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.