Leucine-specific-binding protein precursor - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P04816 (LIVK_ECOLI)

Last modified June 16, 2009. Version 91. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Leucine-specific-binding protein
      Short name=LS-BP
      Short name=L-BP

Gene names

Name:	livK
Ordered Locus Names:	b3458, JW3423

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the leucine-binding protein family.

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Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Periplasm
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

Ref.8

Chain

24 – 369

346

Leucine-specific-binding protein

PRO_0000017702

Amino acid modifications

Disulfide bond

76 ↔ 101

By similarity

Experimental info

Sequence conflict

R → A in CAA23579. Ref.7

Sequence conflict

D → I Ref.1

Sequence conflict

D → I Ref.2

Sequence conflict

D → I Ref.3

Sequence conflict

R → E Ref.1

Sequence conflict

R → E Ref.2

Sequence conflict

R → E Ref.3

Sequence conflict

126

N → A Ref.1

Sequence conflict

126

N → A Ref.2

Sequence conflict

126

N → A Ref.3

Secondary structure

369

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P04816-1 [UniParc].

Last modified July 19, 2003. Version 2.
Checksum: 42B939A18D8723B2
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FASTA

369

39,379

        10         20         30         40         50         60 
MKRNAKTIIA GMIALAISHT AMADDIKVAV VGAMSGPIAQ WGDMEFNGAR QAIKDINAKG 

        70         80         90        100        110        120 
GIKGDKLVGV EYDDACDPKQ AVAVANKIVN DGIKYVIGHL CSSSTQPASD IYEDEGILMI 

       130        140        150        160        170        180 
SPGATNPELT QRGYQHIMRT AGLDSSQGPT AAKYILETVK PQRIAIIHDK QQYGEGLARS 

       190        200        210        220        230        240 
VQDGLKAANA NVVFFDGITA GEKDFSALIA RLKKENIDFV YYGGYYPEMG QMLRQARSVG 

       250        260        270        280        290        300 
LKTQFMGPEG VGNASLSNIA GDAAEGMLVT MPKRYDQDPA NQGIVDALKA DKKDPSGPYV 

       310        320        330        340        350        360 
WITYAAVQSL ATALERTGSD EPLALVKDLK ANGANTVIGP LNWDEKGDLK GFDFGVFQWH 


ADGSSTAAK

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete nucleotide sequences of the Escherichia coli LIV-BP and LS-BP genes. Implications for the mechanism of high-affinity branched-chain amino acid transport." Landick R., Oxender D.L. J. Biol. Chem. 260:8257-8261(1985) [PubMed: 3891753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The leucine binding proteins of Escherichia coli as models for studying the relationships between protein structure and function." Antonucci T.K., Landick R., Oxender D.L. J. Cell. Biochem. 29:209-216(1985) [PubMed: 4077929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli." Adams M.D., Wagner L.M., Graddis T.J., Landick R., Antonucci T.K., Gibson A.L., Oxender D.L. J. Biol. Chem. 265:11436-11443(1990) [PubMed: 2195019] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Amino-terminal sequence and processing of the precursor of the leucine-specific binding protein, and evidence for conformational differences between the precursor and the mature form." Oxender D.L., Anderson J.J., Daniels C.J., Landick R., Gunsalus R.P., Zurawski G., Yanofsky C. Proc. Natl. Acad. Sci. U.S.A. 77:2005-2009(1980) [PubMed: 6990419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36.
[8]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-35. Strain: K12 / EMG2.
[9]	"Structure of the L-leucine-binding protein refined at 2.4-A resolution and comparison with the Leu/Ile/Val-binding protein structure." Sack J.S., Trakhanov S.D., Tsigannik I.H., Quiocho F.A. J. Mol. Biol. 206:193-207(1989) [PubMed: 2649683] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M29378 Genomic DNA. Translation: AAA24090.1.
J05516 Genomic DNA. Translation: AAA83883.1.
U00039 Genomic DNA. Translation: AAB18433.1.
U00096 Genomic DNA. Translation: AAC76483.1.
AP009048 Genomic DNA. Translation: BAE77835.1.
V00301 Genomic DNA. Translation: CAA23579.1.

PIR

BLECL. E65142.

RefSeq

AP_004334.1.
NP_417915.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1USG	X-ray	1.53	A	24-369	[»]
1USI	X-ray	1.80	A/C	24-369	[»]
1USK	X-ray	2.00	A/B/C/D	24-369	[»]
2LBP	X-ray	2.40	A	24-369	[»]

ModBase

Search...

Protein family/group databases

TCDB

3.A.1.4.1. ATP-binding cassette (ABC) superfamily.

2-D gel databases

SWISS-2DPAGE

P04816.

2DBase-Ecoli

P04816.

ECO2DBASE

B040.8. 6TH EDITION.

Genome annotation databases

GeneID

947964.

GenomeReviews

Gene locus JW3423 in contig AP009048_GR.
Gene locus b3458 in contig U00096_GR.

KEGG

ecj:JW3423.
eco:b3458.

Organism-specific databases

EchoBASE

EB0535.

EcoGene

EG10540. livK.

CMR

Search...

Phylogenomic databases

HOGENOM

P04816.

OMA

P04816. YKLIFRT.

Enzyme and pathway databases

BioCyc

EcoCyc:LIVK-MON.

Family and domain databases

InterPro

IPR001828. ANF_lig_bd_rcpt.
IPR000709. Leu_Ile_Val_bd.
[Graphical view]

Pfam

PF01094. ANF_receptor. 1 hit.
[Graphical view]

PRINTS

PR00337. LEUILEVALBP.

ProtoNet

Search...

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Entry information

Entry name

LIVK_ECOLI

Accession

Primary (citable) accession number: P04816
Secondary accession number(s): Q2M7C1, Q47343

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	July 19, 2003
Last modified:	June 16, 2009
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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