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P04814

- TRYA_DROME

UniProt

P04814 - TRYA_DROME

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Protein
Trypsin alpha
Gene
alphaTry, TRY-ALPHA, CG18444
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Charge relay system By similarity
Active sitei116 – 1161Charge relay system By similarity
Sitei204 – 2041Required for specificity By similarity
Active sitei210 – 2101Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: FlyBase
Complete GO annotation...

GO - Biological processi

  1. proteolysis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.110.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin alpha (EC:3.4.21.4)
Gene namesi
Name:alphaTry
Synonyms:TRY-ALPHA
ORF Names:CG18444
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003863. alphaTry.

Subcellular locationi

Secretedextracellular space 1 Publication

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Inferred
Add
BLAST
Propeptidei23 – 308Activation peptide
PRO_0000028261
Chaini31 – 256226Trypsin alpha
PRO_0000028262Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 72 By similarity
Disulfide bondi180 ↔ 197 By similarity
Disulfide bondi206 ↔ 230 By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP04814.

Expressioni

Tissue specificityi

Synthesized in the midgut of both larvae and adults, primarily in the ventriculus and gastric caeca.1 Publication

Gene expression databases

BgeeiP04814.

Interactioni

Protein-protein interaction databases

BioGridi71314. 7 interactions.
DIPiDIP-20031N.
MINTiMINT-1642116.

Structurei

3D structure databases

ProteinModelPortaliP04814.
SMRiP04814. Positions 31-253.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 254224Peptidase S1
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG241902.
GeneTreeiENSGT00620000088101.
InParanoidiP04814.
KOiK01312.
OMAiMICARAT.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP04814.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04814-1 [UniParc]FASTAAdd to Basket

« Hide

MLKIVILLSA VVCALGGTVP EGLLPQLDGR IVGGSATTIS SFPWQISLQR    50
SGSHSCGGSI YSANIIVTAA HCLQSVSASV LQVRAGSTYW SSGGVVAKVS 100
SFKNHEGYNA NTMVNDIAVI RLSSSLSFSS SIKAISLATY NPANGASAAV 150
SGWGTQSSGS SSIPSQLQYV NVNIVSQSQC ASSTYGYGSQ IRNTMICAAA 200
SGKDACQGDS GGPLVSGGVL VGVVSWGYGC AYSNYPGVYA DVAVLRSWVV 250
STANSI 256
Length:256
Mass (Da):26,041
Last modified:August 13, 1987 - v1
Checksum:i8B5634992F2E7C63
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02989 Genomic DNA. Translation: CAA26732.1.
M96372 Genomic DNA. Translation: AAA28982.1.
U04853 Genomic DNA. Translation: AAA17453.1.
AE013599 Genomic DNA. Translation: AAF58659.1.
AY071341 mRNA. Translation: AAL48963.1.
PIRiA23493. TRFF.
RefSeqiNP_476771.1. NM_057423.4.
UniGeneiDm.1525.

Genome annotation databases

EnsemblMetazoaiFBtr0088161; FBpp0087257; FBgn0003863.
GeneIDi48316.
KEGGidme:Dmel_CG18444.
UCSCiCG18444-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02989 Genomic DNA. Translation: CAA26732.1 .
M96372 Genomic DNA. Translation: AAA28982.1 .
U04853 Genomic DNA. Translation: AAA17453.1 .
AE013599 Genomic DNA. Translation: AAF58659.1 .
AY071341 mRNA. Translation: AAL48963.1 .
PIRi A23493. TRFF.
RefSeqi NP_476771.1. NM_057423.4.
UniGenei Dm.1525.

3D structure databases

ProteinModelPortali P04814.
SMRi P04814. Positions 31-253.
ModBasei Search...

Protein-protein interaction databases

BioGridi 71314. 7 interactions.
DIPi DIP-20031N.
MINTi MINT-1642116.

Protein family/group databases

MEROPSi S01.110.

Proteomic databases

PRIDEi P04814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0088161 ; FBpp0087257 ; FBgn0003863 .
GeneIDi 48316.
KEGGi dme:Dmel_CG18444.
UCSCi CG18444-RA. d. melanogaster.

Organism-specific databases

CTDi 48316.
FlyBasei FBgn0003863. alphaTry.

Phylogenomic databases

eggNOGi NOG241902.
GeneTreei ENSGT00620000088101.
InParanoidi P04814.
KOi K01312.
OMAi MICARAT.
OrthoDBi EOG7MKW6Q.
PhylomeDBi P04814.

Miscellaneous databases

GenomeRNAii 48316.
NextBioi 839305.

Gene expression databases

Bgeei P04814.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A gene family in Drosophila melanogaster coding for trypsin-like enzymes."
    Davis C.A., Riddell D.C., Higgins M.J., Holden J.J.A., White B.N.
    Nucleic Acids Res. 13:6605-6619(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Concerted evolution within a trypsin gene cluster in Drosophila."
    Wang S., Magoulas C., Hickey D.A.
    Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Wang S., Magoulas C., Hickey D.A.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  4. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  5. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiTRYA_DROME
AccessioniPrimary (citable) accession number: P04814
Secondary accession number(s): Q541G0, Q9V5Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 9, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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