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Protein

Trypsin alpha

Gene

alphaTry

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Charge relay systemBy similarity
Active sitei116 – 1161Charge relay systemBy similarity
Sitei204 – 2041Required for specificityBy similarity
Active sitei210 – 2101Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: FlyBase

GO - Biological processi

  1. proteolysis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.110.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin alpha (EC:3.4.21.4)
Gene namesi
Name:alphaTry
Synonyms:TRY-ALPHA
ORF Names:CG18444
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003863. alphaTry.

Subcellular locationi

Secretedextracellular space 1 Publication

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222CuratedAdd
BLAST
Propeptidei23 – 308Activation peptidePRO_0000028261
Chaini31 – 256226Trypsin alphaPRO_0000028262Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 72PROSITE-ProRule annotation
Disulfide bondi180 ↔ 197PROSITE-ProRule annotation
Disulfide bondi206 ↔ 230PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP04814.

Expressioni

Tissue specificityi

Synthesized in the midgut of both larvae and adults, primarily in the ventriculus and gastric caeca.1 Publication

Gene expression databases

BgeeiP04814.

Interactioni

Protein-protein interaction databases

BioGridi71314. 7 interactions.
DIPiDIP-20031N.
MINTiMINT-1642116.

Structurei

3D structure databases

ProteinModelPortaliP04814.
SMRiP04814. Positions 31-253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 254224Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG241902.
GeneTreeiENSGT00620000088101.
InParanoidiP04814.
KOiK01312.
OMAiMICARAT.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP04814.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKIVILLSA VVCALGGTVP EGLLPQLDGR IVGGSATTIS SFPWQISLQR
60 70 80 90 100
SGSHSCGGSI YSANIIVTAA HCLQSVSASV LQVRAGSTYW SSGGVVAKVS
110 120 130 140 150
SFKNHEGYNA NTMVNDIAVI RLSSSLSFSS SIKAISLATY NPANGASAAV
160 170 180 190 200
SGWGTQSSGS SSIPSQLQYV NVNIVSQSQC ASSTYGYGSQ IRNTMICAAA
210 220 230 240 250
SGKDACQGDS GGPLVSGGVL VGVVSWGYGC AYSNYPGVYA DVAVLRSWVV

STANSI
Length:256
Mass (Da):26,041
Last modified:August 13, 1987 - v1
Checksum:i8B5634992F2E7C63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02989 Genomic DNA. Translation: CAA26732.1.
M96372 Genomic DNA. Translation: AAA28982.1.
U04853 Genomic DNA. Translation: AAA17453.1.
AE013599 Genomic DNA. Translation: AAF58659.1.
AY071341 mRNA. Translation: AAL48963.1.
PIRiA23493. TRFF.
RefSeqiNP_476771.1. NM_057423.4.
UniGeneiDm.1525.

Genome annotation databases

EnsemblMetazoaiFBtr0088161; FBpp0087257; FBgn0003863.
GeneIDi48316.
KEGGidme:Dmel_CG18444.
UCSCiCG18444-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02989 Genomic DNA. Translation: CAA26732.1.
M96372 Genomic DNA. Translation: AAA28982.1.
U04853 Genomic DNA. Translation: AAA17453.1.
AE013599 Genomic DNA. Translation: AAF58659.1.
AY071341 mRNA. Translation: AAL48963.1.
PIRiA23493. TRFF.
RefSeqiNP_476771.1. NM_057423.4.
UniGeneiDm.1525.

3D structure databases

ProteinModelPortaliP04814.
SMRiP04814. Positions 31-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi71314. 7 interactions.
DIPiDIP-20031N.
MINTiMINT-1642116.

Protein family/group databases

MEROPSiS01.110.

Proteomic databases

PRIDEiP04814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088161; FBpp0087257; FBgn0003863.
GeneIDi48316.
KEGGidme:Dmel_CG18444.
UCSCiCG18444-RA. d. melanogaster.

Organism-specific databases

CTDi48316.
FlyBaseiFBgn0003863. alphaTry.

Phylogenomic databases

eggNOGiNOG241902.
GeneTreeiENSGT00620000088101.
InParanoidiP04814.
KOiK01312.
OMAiMICARAT.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP04814.

Miscellaneous databases

GenomeRNAii48316.
NextBioi839305.

Gene expression databases

BgeeiP04814.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A gene family in Drosophila melanogaster coding for trypsin-like enzymes."
    Davis C.A., Riddell D.C., Higgins M.J., Holden J.J.A., White B.N.
    Nucleic Acids Res. 13:6605-6619(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Concerted evolution within a trypsin gene cluster in Drosophila."
    Wang S., Magoulas C., Hickey D.A.
    Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Wang S., Magoulas C., Hickey D.A.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  4. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  5. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiTRYA_DROME
AccessioniPrimary (citable) accession number: P04814
Secondary accession number(s): Q541G0, Q9V5Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: February 4, 2015
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.