Reviewed, UniProtKB/Swiss-Prot P04813 (CTR2_CANFA)

Names and origin

Protein names

Recommended name:
    Chymotrypsinogen 2
    EC=3.4.21.1
Cleaved into the following 3 chains:
    1- Recommended name:
            Chymotrypsin 2 chain A
    2- Recommended name:
            Chymotrypsin 2 chain B
    3- Recommended name:
            Chymotrypsin 2 chain C

Gene names

Name:	CTRB1
Synonyms:	CTRB

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18
	Chain	19 – 263	245	Chymotrypsinogen 2		PRO_0000027634
	Chain	19 – 31	13	Chymotrypsin 2 chain A		PRO_0000027635
	Chain	34 – 164	131	Chymotrypsin 2 chain B		PRO_0000027636
	Chain	167 – 263	97	Chymotrypsin 2 chain C		PRO_0000027637
Regions
	Domain	34 – 261	228	Peptidase S1
Sites
	Active site	75	1	Charge relay system
	Active site	120	1	Charge relay system
	Active site	213	1	Charge relay system
Amino acid modifications
	Disulfide bond	19 ↔ 140		By similarity
	Disulfide bond	60 ↔ 76		By similarity
	Disulfide bond	154 ↔ 219		By similarity
	Disulfide bond	186 ↔ 200		By similarity
	Disulfide bond	209 ↔ 238		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04813-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 2A2F449D813B3961	FASTA	263	27,787
	10 20 30 40 50 60 MAFLWLLSCF ALLGTAFGCG VPAIQPVLSG LSRIVNGEDA VPGSWPWQVS LQDSTGFHFC 70 80 90 100 110 120 GGSLISEDWV VTAAHCGVRT THQVVAGEFD QGSDAESIQV LKIAKVFKNP KFNMFTINND 130 140 150 160 170 180 ITLLKLATPA RFSKTVSAVC LPQATDDFPA GTLCVTTGWG LTKHTNANTP DKLQQAALPL 190 200 210 220 230 240 LSNAECKKFW GSKITDLMVC AGASGVSSCM GDSGGPLVCQ KDGAWTLVGI VSWGSGTCST 250 260 STPGVYARVT KLIPWVQQIL QAN

References

[1]

"Identification of cDNA clones encoding secretory isoenzyme forms: sequence determination of canine pancreatic prechymotrypsinogen 2 mRNA." Pinsky S.D., Laforge K.S., Luc V., Scheele G. Proc. Natl. Acad. Sci. U.S.A. 80:7486-7490(1983) [PubMed: 6584866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	K01173 mRNA. Translation: AAA30841.1.
PIR	A21195.
RefSeq	XP_536781.2.
UniGene	Cfa.40446
3D structure databases
HSSP	HSSP built from PDB template 1ACB based on UniProtKB P00766.
SMR	P04813. Positions 19-263.
ModBase	Search...
Protein family/group databases
MEROPS	S01.152.
Genome annotation databases
Ensembl	ENSCAFG00000020084. Canis familiaris. [Contig view]
GeneID	479649.
KEGG	cfa:479649.
Phylogenomic databases
HOVERGEN	P04813.
OMA	P04813. HCGVRTT.
Enzyme and pathway databases
BRENDA	3.4.21.1. 463.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTR2_CANFA

Accession

Primary (citable) accession number: P04813

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)