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P04807

- HXKB_YEAST

UniProt

P04807 - HXKB_YEAST

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Protein
Hexokinase-2
Gene
HXK2, HEX1, HKB, YGL253W, NRB486
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Main glucose phosphorylating enzyme. May play a regulatory role in both induction and repression of gene expression by glucose.

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Subject to allosteric control. Substrate inhibition by ATP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111ATP Reviewed prediction
Binding sitei158 – 1581Substrate; via carbonyl oxygen By similarity
Binding sitei237 – 2371Substrate By similarity
Binding sitei269 – 2691Substrate By similarity
Binding sitei302 – 3021Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 916ATP By similarity
Nucleotide bindingi307 – 3082ATP By similarity
Nucleotide bindingi344 – 3485ATP By similarity
Nucleotide bindingi419 – 4235ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hexokinase activity Source: SGD

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose import Source: SGD
  3. fructose metabolic process Source: SGD
  4. glucose import Source: SGD
  5. glucose metabolic process Source: SGD
  6. glycolytic process Source: SGD
  7. mannose metabolic process Source: SGD
  8. regulation of transcription by glucose Source: SGD
  9. replicative cell aging Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YGL253W-MONOMER.
BRENDAi2.7.1.1. 984.
SABIO-RKP04807.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Alternative name(s):
Hexokinase PII
Hexokinase-B
Gene namesi
Name:HXK2
Synonyms:HEX1, HKB
Ordered Locus Names:YGL253W
ORF Names:NRB486
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

SGDiS000003222. HXK2.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
  2. mitochondrion Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 486485Hexokinase-2
PRO_0000197602Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151Phosphoserine3 Publications
Modified residuei38 – 381Phosphothreonine1 Publication
Modified residuei158 – 1581Phosphoserine2 Publications
Modified residuei245 – 2451Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04807.
PaxDbiP04807.
PeptideAtlasiP04807.

2D gel databases

SWISS-2DPAGEP04807.

Expressioni

Gene expression databases

GenevestigatoriP04807.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi33026. 150 interactions.
DIPiDIP-2380N.
IntActiP04807. 3 interactions.
MINTiMINT-2784874.
STRINGi4932.YGL253W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 3414
Helixi38 – 5619
Beta strandi79 – 879
Beta strandi89 – 10315
Beta strandi105 – 1139
Helixi118 – 1203
Helixi126 – 14217
Beta strandi152 – 1565
Beta strandi159 – 1624
Helixi189 – 20012
Beta strandi203 – 2097
Helixi211 – 22212
Beta strandi226 – 24217
Helixi244 – 2463
Helixi248 – 2503
Beta strandi254 – 2563
Beta strandi264 – 2674
Turni271 – 2766
Beta strandi278 – 2803
Helixi284 – 2929
Beta strandi293 – 2953
Helixi300 – 3056
Helixi307 – 3093
Helixi310 – 32314
Beta strandi326 – 3305
Turni334 – 3363
Helixi345 – 3528
Beta strandi355 – 3573
Helixi359 – 36911
Helixi375 – 39622
Helixi398 – 40710
Beta strandi410 – 4189
Helixi419 – 4235
Helixi427 – 43913
Helixi446 – 4483
Beta strandi449 – 4557
Turni459 – 4613
Helixi462 – 47110

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IG8X-ray2.20A1-486[»]
2YHXX-ray2.10A152-471[»]
ProteinModelPortaliP04807.
SMRiP04807. Positions 18-486.

Miscellaneous databases

EvolutionaryTraceiP04807.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 223203Hexokinase type-1
Add
BLAST
Domaini225 – 473249Hexokinase type-2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 1762Substrate binding By similarity
Regioni210 – 2112Substrate binding By similarity

Sequence similaritiesi

Belongs to the hexokinase family.

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
KOiK00844.
OMAiPEMKIGC.
OrthoDBiEOG79SF68.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04807-1 [UniParc]FASTAAdd to Basket

« Hide

MVHLGPKKPQ ARKGSMADVP KELMQQIENF EKIFTVPTET LQAVTKHFIS    50
ELEKGLSKKG GNIPMIPGWV MDFPTGKESG DFLAIDLGGT NLRVVLVKLG 100
GDRTFDTTQS KYRLPDAMRT TQNPDELWEF IADSLKAFID EQFPQGISEP 150
IPLGFTFSFP ASQNKINEGI LQRWTKGFDI PNIENHDVVP MLQKQITKRN 200
IPIEVVALIN DTTGTLVASY YTDPETKMGV IFGTGVNGAY YDVCSDIEKL 250
QGKLSDDIPP SAPMAINCEY GSFDNEHVVL PRTKYDITID EESPRPGQQT 300
FEKMSSGYYL GEILRLALMD MYKQGFIFKN QDLSKFDKPF VMDTSYPARI 350
EEDPFENLED TDDLFQNEFG INTTVQERKL IRRLSELIGA RAARLSVCGI 400
AAICQKRGYK TGHIAADGSV YNRYPGFKEK AANALKDIYG WTQTSLDDYP 450
IKIVPAEDGS GAGAAVIAAL AQKRIAEGKS VGIIGA 486
Length:486
Mass (Da):53,942
Last modified:January 23, 2007 - v4
Checksum:iD55FF3F8992B2FEF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291N → I in CAA27203. 1 Publication
Sequence conflicti33 – 331I → N in AAA34697. 1 Publication
Sequence conflicti33 – 331I → N in AAA34699. 1 Publication
Sequence conflicti61 – 611G → V in CAA27203. 1 Publication
Sequence conflicti197 – 1971T → S in CAA27203. 1 Publication
Sequence conflicti202 – 2021P → H in AAA34699. 1 Publication
Sequence conflicti421 – 4222YN → ST in AAA34697. 1 Publication
Sequence conflicti421 – 4222YN → ST in AAA34699. 1 Publication
Sequence conflicti444 – 4452TS → PH in AAA34697. 1 Publication
Sequence conflicti444 – 4452TS → PH in AAA34699. 1 Publication
Sequence conflicti453 – 4531I → V in AAA34697. 1 Publication
Sequence conflicti453 – 4531I → V in AAA34699. 1 Publication
Sequence conflicti462 – 4621A → P in AAA34697. 1 Publication
Sequence conflicti462 – 4621A → P in AAA34699. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03483 Genomic DNA. Translation: CAA27203.1.
M11181 Genomic DNA. Translation: AAA34697.1.
M14411 mRNA. Translation: AAA34699.1.
X94357 Genomic DNA. Translation: CAA64134.1.
Z72775 Genomic DNA. Translation: CAA96973.1.
X67787 Genomic DNA. Translation: CAA48003.1.
BK006941 Genomic DNA. Translation: DAA07866.1.
PIRiS61608. KIBYHB.
RefSeqiNP_011261.1. NM_001181119.1.

Genome annotation databases

EnsemblFungiiYGL253W; YGL253W; YGL253W.
GeneIDi852639.
KEGGisce:YGL253W.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03483 Genomic DNA. Translation: CAA27203.1 .
M11181 Genomic DNA. Translation: AAA34697.1 .
M14411 mRNA. Translation: AAA34699.1 .
X94357 Genomic DNA. Translation: CAA64134.1 .
Z72775 Genomic DNA. Translation: CAA96973.1 .
X67787 Genomic DNA. Translation: CAA48003.1 .
BK006941 Genomic DNA. Translation: DAA07866.1 .
PIRi S61608. KIBYHB.
RefSeqi NP_011261.1. NM_001181119.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IG8 X-ray 2.20 A 1-486 [» ]
2YHX X-ray 2.10 A 152-471 [» ]
ProteinModelPortali P04807.
SMRi P04807. Positions 18-486.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33026. 150 interactions.
DIPi DIP-2380N.
IntActi P04807. 3 interactions.
MINTi MINT-2784874.
STRINGi 4932.YGL253W.

2D gel databases

SWISS-2DPAGE P04807.

Proteomic databases

MaxQBi P04807.
PaxDbi P04807.
PeptideAtlasi P04807.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL253W ; YGL253W ; YGL253W .
GeneIDi 852639.
KEGGi sce:YGL253W.

Organism-specific databases

SGDi S000003222. HXK2.

Phylogenomic databases

eggNOGi COG5026.
GeneTreei ENSGT00390000017159.
HOGENOMi HOG000162670.
KOi K00844.
OMAi PEMKIGC.
OrthoDBi EOG79SF68.

Enzyme and pathway databases

UniPathwayi UPA00242 .
BioCyci YEAST:YGL253W-MONOMER.
BRENDAi 2.7.1.1. 984.
SABIO-RK P04807.

Miscellaneous databases

EvolutionaryTracei P04807.
NextBioi 971888.
PROi P04807.

Gene expression databases

Genevestigatori P04807.

Family and domain databases

InterProi IPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view ]
PANTHERi PTHR19443. PTHR19443. 1 hit.
Pfami PF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view ]
PRINTSi PR00475. HEXOKINASE.
PROSITEi PS00378. HEXOKINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification, cloning and sequence determination of the genes specifying hexokinase A and B from yeast."
    Stachelek C., Stachelek J., Swan J., Botstein D., Konigsberg W.
    Nucleic Acids Res. 14:945-963(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The primary structure of the yeast hexokinase PII gene (HXK2) which is responsible for glucose repression."
    Froehlich K.-U., Entian K.-D., Mecke D.
    Gene 36:105-111(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae."
    Coissac E., Maillier E., Robineau S., Netter P.
    Yeast 12:1555-1562(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Identification of a gene encoding a novel zinc finger protein in Saccharomyces cerevisiae."
    Breitwieser W., Price C., Schuster T.
    Yeast 9:551-556(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-247.
    Strain: ATCC 200060 / W303.
  7. "Gene linkage of two-dimensional polyacrylamide gel electrophoresis resolved proteins from isogene families in Saccharomyces cerevisiae by microsequencing of in-gel trypsin generated peptides."
    Norbeck J., Blomberg A.
    Electrophoresis 16:149-156(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 119-127; 176-185 AND 304-314.
    Strain: ATCC 38531 / Y41.
  8. "In vivo phosphorylation site of hexokinase 2 in Saccharomyces cerevisiae."
    Kriegel T.M., Rush J., Vojtek A.B., Clifton D., Fraenkel D.G.
    Biochemistry 33:148-152(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-15.
  9. "Autophosphorylation-inactivation site of hexokinase 2 in Saccharomyces cerevisiae."
    Heidrich K., Otto A., Behlke J., Rush J., Wenzel K.W., Kriegel T.
    Biochemistry 36:1960-1964(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-158.
  10. "Hexokinase 2 from Saccharomyces cerevisiae: regulation of oligomeric structure by in vivo phosphorylation at serine-14."
    Behlke J., Heidrich K., Naumann M., Mueller E.-C., Otto A., Reuter R., Kriegel T.
    Biochemistry 37:11989-11995(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-19, PHOSPHORYLATION AT SER-15.
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-38 AND SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15 AND SER-245, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Sequencing a protein by X-ray crystallography. II. Refinement of yeast hexokinase B co-ordinates and sequence at 2.1-A resolution."
    Anderson C.M., Stenkamp R.E., Steitz T.A.
    J. Mol. Biol. 123:15-33(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Entry informationi

Entry nameiHXKB_YEAST
AccessioniPrimary (citable) accession number: P04807
Secondary accession number(s): D6VV82, Q05838
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 153 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase.
Present with 114000 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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