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Protein

Hexokinase-2

Gene

HXK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Main glucose phosphorylating enzyme. May play a regulatory role in both induction and repression of gene expression by glucose.

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Subject to allosteric control. Substrate inhibition by ATP.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111ATPSequence analysis1
Binding sitei158Substrate; via carbonyl oxygenBy similarity1
Binding sitei237SubstrateBy similarity1
Binding sitei269SubstrateBy similarity1
Binding sitei302SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 91ATPBy similarity6
Nucleotide bindingi307 – 308ATPBy similarity2
Nucleotide bindingi344 – 348ATPBy similarity5
Nucleotide bindingi419 – 423ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • cellular glucose homeostasis Source: GO_Central
  • fructose import Source: SGD
  • fructose metabolic process Source: SGD
  • glucose import Source: SGD
  • glucose metabolic process Source: SGD
  • glycolytic process Source: SGD
  • mannose metabolic process Source: SGD
  • regulation of transcription by glucose Source: SGD
  • replicative cell aging Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YGL253W-MONOMER.
BRENDAi2.7.1.1. 984.
SABIO-RKP04807.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-2 (EC:2.7.1.1)
Alternative name(s):
Hexokinase PII
Hexokinase-B
Gene namesi
Name:HXK2
Synonyms:HEX1, HKB
Ordered Locus Names:YGL253W
ORF Names:NRB486
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL253W.
SGDiS000003222. HXK2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • mitochondrion Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001976022 – 486Hexokinase-2Add BLAST485

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources2 Publications1
Modified residuei38PhosphothreonineCombined sources1
Modified residuei158PhosphoserineCombined sources1 Publication1
Modified residuei245PhosphoserineCombined sources1
Modified residuei272PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04807.
PRIDEiP04807.
TopDownProteomicsiP04807.

2D gel databases

SWISS-2DPAGEP04807.

PTM databases

iPTMnetiP04807.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi33026. 155 interactors.
DIPiDIP-2380N.
IntActiP04807. 3 interactors.
MINTiMINT-2784874.

Structurei

Secondary structure

1486
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 34Combined sources14
Helixi38 – 56Combined sources19
Beta strandi79 – 87Combined sources9
Beta strandi89 – 103Combined sources15
Beta strandi105 – 113Combined sources9
Helixi118 – 120Combined sources3
Helixi126 – 142Combined sources17
Beta strandi152 – 156Combined sources5
Beta strandi159 – 162Combined sources4
Helixi189 – 200Combined sources12
Beta strandi203 – 209Combined sources7
Helixi211 – 222Combined sources12
Beta strandi226 – 242Combined sources17
Helixi244 – 246Combined sources3
Helixi248 – 250Combined sources3
Beta strandi254 – 256Combined sources3
Beta strandi264 – 267Combined sources4
Turni271 – 276Combined sources6
Beta strandi278 – 280Combined sources3
Helixi284 – 292Combined sources9
Beta strandi293 – 295Combined sources3
Helixi300 – 305Combined sources6
Helixi307 – 309Combined sources3
Helixi310 – 323Combined sources14
Beta strandi326 – 330Combined sources5
Turni334 – 336Combined sources3
Helixi345 – 352Combined sources8
Beta strandi355 – 357Combined sources3
Helixi359 – 369Combined sources11
Helixi375 – 396Combined sources22
Helixi398 – 407Combined sources10
Beta strandi410 – 418Combined sources9
Helixi419 – 423Combined sources5
Helixi427 – 439Combined sources13
Helixi446 – 448Combined sources3
Beta strandi449 – 455Combined sources7
Turni459 – 461Combined sources3
Helixi462 – 471Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IG8X-ray2.20A1-486[»]
2YHXX-ray2.10A152-471[»]
ProteinModelPortaliP04807.
SMRiP04807.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04807.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 469HexokinasePROSITE-ProRule annotationAdd BLAST449

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni75 – 209Hexokinase small subdomainPROSITE-ProRule annotationAdd BLAST135
Regioni175 – 176Substrate bindingBy similarity2
Regioni210 – 458Hexokinase large subdomainPROSITE-ProRule annotationAdd BLAST249
Regioni210 – 211Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 1 hexokinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
InParanoidiP04807.
KOiK00844.
OMAiGWVMDFP.
OrthoDBiEOG092C2JW4.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04807-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHLGPKKPQ ARKGSMADVP KELMQQIENF EKIFTVPTET LQAVTKHFIS
60 70 80 90 100
ELEKGLSKKG GNIPMIPGWV MDFPTGKESG DFLAIDLGGT NLRVVLVKLG
110 120 130 140 150
GDRTFDTTQS KYRLPDAMRT TQNPDELWEF IADSLKAFID EQFPQGISEP
160 170 180 190 200
IPLGFTFSFP ASQNKINEGI LQRWTKGFDI PNIENHDVVP MLQKQITKRN
210 220 230 240 250
IPIEVVALIN DTTGTLVASY YTDPETKMGV IFGTGVNGAY YDVCSDIEKL
260 270 280 290 300
QGKLSDDIPP SAPMAINCEY GSFDNEHVVL PRTKYDITID EESPRPGQQT
310 320 330 340 350
FEKMSSGYYL GEILRLALMD MYKQGFIFKN QDLSKFDKPF VMDTSYPARI
360 370 380 390 400
EEDPFENLED TDDLFQNEFG INTTVQERKL IRRLSELIGA RAARLSVCGI
410 420 430 440 450
AAICQKRGYK TGHIAADGSV YNRYPGFKEK AANALKDIYG WTQTSLDDYP
460 470 480
IKIVPAEDGS GAGAAVIAAL AQKRIAEGKS VGIIGA
Length:486
Mass (Da):53,942
Last modified:January 23, 2007 - v4
Checksum:iD55FF3F8992B2FEF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29N → I in CAA27203 (PubMed:3003701).Curated1
Sequence conflicti33I → N in AAA34697 (PubMed:3905511).Curated1
Sequence conflicti33I → N in AAA34699 (PubMed:3905511).Curated1
Sequence conflicti61G → V in CAA27203 (PubMed:3003701).Curated1
Sequence conflicti197T → S in CAA27203 (PubMed:3003701).Curated1
Sequence conflicti202P → H in AAA34699 (PubMed:3905511).Curated1
Sequence conflicti421 – 422YN → ST in AAA34697 (PubMed:3905511).Curated2
Sequence conflicti421 – 422YN → ST in AAA34699 (PubMed:3905511).Curated2
Sequence conflicti444 – 445TS → PH in AAA34697 (PubMed:3905511).Curated2
Sequence conflicti444 – 445TS → PH in AAA34699 (PubMed:3905511).Curated2
Sequence conflicti453I → V in AAA34697 (PubMed:3905511).Curated1
Sequence conflicti453I → V in AAA34699 (PubMed:3905511).Curated1
Sequence conflicti462A → P in AAA34697 (PubMed:3905511).Curated1
Sequence conflicti462A → P in AAA34699 (PubMed:3905511).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03483 Genomic DNA. Translation: CAA27203.1.
M11181 Genomic DNA. Translation: AAA34697.1.
M14411 mRNA. Translation: AAA34699.1.
X94357 Genomic DNA. Translation: CAA64134.1.
Z72775 Genomic DNA. Translation: CAA96973.1.
X67787 Genomic DNA. Translation: CAA48003.1.
BK006941 Genomic DNA. Translation: DAA07866.1.
PIRiS61608. KIBYHB.
RefSeqiNP_011261.1. NM_001181119.1.

Genome annotation databases

EnsemblFungiiYGL253W; YGL253W; YGL253W.
GeneIDi852639.
KEGGisce:YGL253W.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03483 Genomic DNA. Translation: CAA27203.1.
M11181 Genomic DNA. Translation: AAA34697.1.
M14411 mRNA. Translation: AAA34699.1.
X94357 Genomic DNA. Translation: CAA64134.1.
Z72775 Genomic DNA. Translation: CAA96973.1.
X67787 Genomic DNA. Translation: CAA48003.1.
BK006941 Genomic DNA. Translation: DAA07866.1.
PIRiS61608. KIBYHB.
RefSeqiNP_011261.1. NM_001181119.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IG8X-ray2.20A1-486[»]
2YHXX-ray2.10A152-471[»]
ProteinModelPortaliP04807.
SMRiP04807.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33026. 155 interactors.
DIPiDIP-2380N.
IntActiP04807. 3 interactors.
MINTiMINT-2784874.

PTM databases

iPTMnetiP04807.

2D gel databases

SWISS-2DPAGEP04807.

Proteomic databases

MaxQBiP04807.
PRIDEiP04807.
TopDownProteomicsiP04807.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL253W; YGL253W; YGL253W.
GeneIDi852639.
KEGGisce:YGL253W.

Organism-specific databases

EuPathDBiFungiDB:YGL253W.
SGDiS000003222. HXK2.

Phylogenomic databases

GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
InParanoidiP04807.
KOiK00844.
OMAiGWVMDFP.
OrthoDBiEOG092C2JW4.

Enzyme and pathway databases

UniPathwayiUPA00242.
BioCyciYEAST:YGL253W-MONOMER.
BRENDAi2.7.1.1. 984.
SABIO-RKP04807.

Miscellaneous databases

EvolutionaryTraceiP04807.
PROiP04807.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHXKB_YEAST
AccessioniPrimary (citable) accession number: P04807
Secondary accession number(s): D6VV82, Q05838
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 177 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase.
Present with 114000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.