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Protein

Hexokinase-1

Gene

HXK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Subject to allosteric control. Substrate inhibition by ATP.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111ATPSequence analysis1
Binding sitei158Substrate; via carbonyl oxygen1
Binding sitei237Substrate1
Binding sitei269Substrate1
Binding sitei302Substrate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 91ATPBy similarity6
Nucleotide bindingi307 – 308ATPBy similarity2
Nucleotide bindingi344 – 348ATPBy similarity5
Nucleotide bindingi419 – 423ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • cellular glucose homeostasis Source: GO_Central
  • fructose import Source: SGD
  • fructose metabolic process Source: SGD
  • glucose import Source: SGD
  • glucose metabolic process Source: SGD
  • glycolytic process Source: SGD
  • mannose metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YFR053C-MONOMER.
BRENDAi2.7.1.1. 984.
ReactomeiR-SCE-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-70153. Glucose transport.
R-SCE-70171. Glycolysis.
SABIO-RKP04806.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-1 (EC:2.7.1.1)
Alternative name(s):
Hexokinase PI
Hexokinase-A
Gene namesi
Name:HXK1
Synonyms:HKA
Ordered Locus Names:YFR053C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR053C.
SGDiS000001949. HXK1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001976011 – 485Hexokinase-1Add BLAST485

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei245PhosphoserineCombined sources1
Modified residuei272PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04806.
PRIDEiP04806.
TopDownProteomicsiP04806.

2D gel databases

SWISS-2DPAGEP04806.

PTM databases

iPTMnetiP04806.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi31211. 58 interactors.
DIPiDIP-5377N.
IntActiP04806. 2 interactors.
MINTiMINT-561285.

Chemistry databases

BindingDBiP04806.

Structurei

Secondary structure

1485
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 34Combined sources14
Helixi38 – 54Combined sources17
Beta strandi57 – 59Combined sources3
Beta strandi80 – 87Combined sources8
Beta strandi89 – 99Combined sources11
Beta strandi101 – 113Combined sources13
Helixi116 – 120Combined sources5
Helixi125 – 141Combined sources17
Beta strandi151 – 156Combined sources6
Helixi181 – 183Combined sources3
Helixi188 – 196Combined sources9
Helixi197 – 199Combined sources3
Beta strandi202 – 209Combined sources8
Helixi211 – 220Combined sources10
Beta strandi226 – 243Combined sources18
Helixi244 – 246Combined sources3
Helixi248 – 250Combined sources3
Beta strandi263 – 267Combined sources5
Helixi270 – 272Combined sources3
Turni273 – 276Combined sources4
Beta strandi278 – 280Combined sources3
Helixi284 – 292Combined sources9
Beta strandi293 – 295Combined sources3
Helixi300 – 303Combined sources4
Helixi307 – 322Combined sources16
Turni323 – 325Combined sources3
Beta strandi326 – 328Combined sources3
Turni334 – 337Combined sources4
Helixi345 – 351Combined sources7
Helixi359 – 367Combined sources9
Helixi375 – 396Combined sources22
Helixi398 – 406Combined sources9
Beta strandi410 – 417Combined sources8
Helixi419 – 423Combined sources5
Helixi427 – 439Combined sources13
Turni444 – 446Combined sources3
Beta strandi449 – 454Combined sources6
Turni458 – 460Combined sources3
Helixi461 – 475Combined sources15
Beta strandi480 – 482Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HKGX-ray3.50A152-466[»]
3B8AX-ray2.95X1-485[»]
ProteinModelPortaliP04806.
SMRiP04806.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04806.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 468HexokinasePROSITE-ProRule annotationAdd BLAST448

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni75 – 209Hexokinase small subdomainPROSITE-ProRule annotationAdd BLAST135
Regioni175 – 176Substrate binding2
Regioni210 – 457Hexokinase large subdomainPROSITE-ProRule annotationAdd BLAST248
Regioni210 – 211Substrate binding2

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 1 hexokinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00860000135274.
HOGENOMiHOG000162670.
InParanoidiP04806.
KOiK00844.
OMAiYIMDTSY.
OrthoDBiEOG092C2JW4.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHLGPKKPQ ARKGSMADVP KELMDEIHQL EDMFTVDSET LRKVVKHFID
60 70 80 90 100
ELNKGLTKKG GNIPMIPGWV MEFPTGKESG NYLAIDLGGT NLRVVLVKLS
110 120 130 140 150
GNHTFDTTQS KYKLPHDMRT TKHQEELWSF IADSLKDFMV EQELLNTKDT
160 170 180 190 200
LPLGFTFSYP ASQNKINEGI LQRWTKGFDI PNVEGHDVVP LLQNEISKRE
210 220 230 240 250
LPIEIVALIN DTVGTLIASY YTDPETKMGV IFGTGVNGAF YDVVSDIEKL
260 270 280 290 300
EGKLADDIPS NSPMAINCEY GSFDNEHLVL PRTKYDVAVD EQSPRPGQQA
310 320 330 340 350
FEKMTSGYYL GELLRLVLLE LNEKGLMLKD QDLSKLKQPY IMDTSYPARI
360 370 380 390 400
EDDPFENLED TDDIFQKDFG VKTTLPERKL IRRLCELIGT RAARLAVCGI
410 420 430 440 450
AAICQKRGYK TGHIAADGSV YNKYPGFKEA AAKGLRDIYG WTGDASKDPI
460 470 480
TIVPAEDGSG AGAAVIAALS EKRIAEGKSL GIIGA
Length:485
Mass (Da):53,738
Last modified:November 1, 1995 - v2
Checksum:iAF5C9DA8F17BC3D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61G → V in CAA27202 (PubMed:3003701).Curated1
Sequence conflicti103H → R in CAA27202 (PubMed:3003701).Curated1
Sequence conflicti194N → K in CAA27202 (PubMed:3003701).Curated1
Sequence conflicti244V → C in CAA27202 (PubMed:3003701).Curated1
Sequence conflicti356 – 357EN → VF in AAA34698 (PubMed:3908224).Curated2
Sequence conflicti364I → M in CAA27202 (PubMed:3003701).Curated1
Sequence conflicti388I → T in AAA34698 (PubMed:3908224).Curated1
Sequence conflicti444D → EN in CAA27202 (PubMed:3003701).Curated1
Sequence conflicti479 – 480SL → VS in CAA27202 (PubMed:3003701).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14410 mRNA. Translation: AAA34698.1.
X03482 Genomic DNA. Translation: CAA27202.1.
D50617 Genomic DNA. Translation: BAA09292.1.
BK006940 Genomic DNA. Translation: DAA12496.1.
PIRiS56308. KIBYHA.
RefSeqiNP_116711.3. NM_001180018.3.

Genome annotation databases

EnsemblFungiiBAA09292; BAA09292; BAA09292.
YFR053C; YFR053C; YFR053C.
GeneIDi850614.
KEGGisce:YFR053C.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14410 mRNA. Translation: AAA34698.1.
X03482 Genomic DNA. Translation: CAA27202.1.
D50617 Genomic DNA. Translation: BAA09292.1.
BK006940 Genomic DNA. Translation: DAA12496.1.
PIRiS56308. KIBYHA.
RefSeqiNP_116711.3. NM_001180018.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HKGX-ray3.50A152-466[»]
3B8AX-ray2.95X1-485[»]
ProteinModelPortaliP04806.
SMRiP04806.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31211. 58 interactors.
DIPiDIP-5377N.
IntActiP04806. 2 interactors.
MINTiMINT-561285.

Chemistry databases

BindingDBiP04806.

PTM databases

iPTMnetiP04806.

2D gel databases

SWISS-2DPAGEP04806.

Proteomic databases

MaxQBiP04806.
PRIDEiP04806.
TopDownProteomicsiP04806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09292; BAA09292; BAA09292.
YFR053C; YFR053C; YFR053C.
GeneIDi850614.
KEGGisce:YFR053C.

Organism-specific databases

EuPathDBiFungiDB:YFR053C.
SGDiS000001949. HXK1.

Phylogenomic databases

GeneTreeiENSGT00860000135274.
HOGENOMiHOG000162670.
InParanoidiP04806.
KOiK00844.
OMAiYIMDTSY.
OrthoDBiEOG092C2JW4.

Enzyme and pathway databases

UniPathwayiUPA00242.
BioCyciYEAST:YFR053C-MONOMER.
BRENDAi2.7.1.1. 984.
ReactomeiR-SCE-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-SCE-6798695. Neutrophil degranulation.
R-SCE-70153. Glucose transport.
R-SCE-70171. Glycolysis.
SABIO-RKP04806.

Miscellaneous databases

EvolutionaryTraceiP04806.
PROiP04806.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHXKA_YEAST
AccessioniPrimary (citable) accession number: P04806
Secondary accession number(s): D6VTT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 180 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase.
Present with 40800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.