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Protein

Hexokinase-1

Gene

HXK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Subject to allosteric control. Substrate inhibition by ATP.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei111 – 1111ATPSequence analysis
Binding sitei158 – 1581Substrate; via carbonyl oxygen
Binding sitei237 – 2371Substrate
Binding sitei269 – 2691Substrate
Binding sitei302 – 3021Substrate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 916ATPBy similarity
Nucleotide bindingi307 – 3082ATPBy similarity
Nucleotide bindingi344 – 3485ATPBy similarity
Nucleotide bindingi419 – 4235ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellular glucose homeostasis Source: GO_Central
  • fructose import Source: SGD
  • fructose metabolic process Source: SGD
  • glucose import Source: SGD
  • glucose metabolic process Source: SGD
  • glycolytic process Source: SGD
  • mannose metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YFR053C-MONOMER.
BRENDAi2.7.1.1. 984.
ReactomeiR-SCE-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-SCE-70153. Glucose transport.
R-SCE-70171. Glycolysis.
SABIO-RKP04806.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-1 (EC:2.7.1.1)
Alternative name(s):
Hexokinase PI
Hexokinase-A
Gene namesi
Name:HXK1
Synonyms:HKA
Ordered Locus Names:YFR053C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR053C.
SGDiS000001949. HXK1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Hexokinase-1PRO_0000197601Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei245 – 2451PhosphoserineCombined sources
Modified residuei272 – 2721PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04806.
TopDownProteomicsiP04806.

2D gel databases

SWISS-2DPAGEP04806.

PTM databases

iPTMnetiP04806.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi31211. 58 interactions.
DIPiDIP-5377N.
IntActiP04806. 2 interactions.
MINTiMINT-561285.

Chemistry

BindingDBiP04806.

Structurei

Secondary structure

1
485
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 3414Combined sources
Helixi38 – 5417Combined sources
Beta strandi57 – 593Combined sources
Beta strandi80 – 878Combined sources
Beta strandi89 – 9911Combined sources
Beta strandi101 – 11313Combined sources
Helixi116 – 1205Combined sources
Helixi125 – 14117Combined sources
Beta strandi151 – 1566Combined sources
Helixi181 – 1833Combined sources
Helixi188 – 1969Combined sources
Helixi197 – 1993Combined sources
Beta strandi202 – 2098Combined sources
Helixi211 – 22010Combined sources
Beta strandi226 – 24318Combined sources
Helixi244 – 2463Combined sources
Helixi248 – 2503Combined sources
Beta strandi263 – 2675Combined sources
Helixi270 – 2723Combined sources
Turni273 – 2764Combined sources
Beta strandi278 – 2803Combined sources
Helixi284 – 2929Combined sources
Beta strandi293 – 2953Combined sources
Helixi300 – 3034Combined sources
Helixi307 – 32216Combined sources
Turni323 – 3253Combined sources
Beta strandi326 – 3283Combined sources
Turni334 – 3374Combined sources
Helixi345 – 3517Combined sources
Helixi359 – 3679Combined sources
Helixi375 – 39622Combined sources
Helixi398 – 4069Combined sources
Beta strandi410 – 4178Combined sources
Helixi419 – 4235Combined sources
Helixi427 – 43913Combined sources
Turni444 – 4463Combined sources
Beta strandi449 – 4546Combined sources
Turni458 – 4603Combined sources
Helixi461 – 47515Combined sources
Beta strandi480 – 4823Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HKGX-ray3.50A152-466[»]
3B8AX-ray2.95X1-485[»]
ProteinModelPortaliP04806.
SMRiP04806. Positions 15-485.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04806.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 468448HexokinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni75 – 209135Hexokinase small subdomainPROSITE-ProRule annotationAdd
BLAST
Regioni175 – 1762Substrate binding
Regioni210 – 457248Hexokinase large subdomainPROSITE-ProRule annotationAdd
BLAST
Regioni210 – 2112Substrate binding

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 1 hexokinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
InParanoidiP04806.
KOiK00844.
OMAiYIMDTSY.
OrthoDBiEOG092C2JW4.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHLGPKKPQ ARKGSMADVP KELMDEIHQL EDMFTVDSET LRKVVKHFID
60 70 80 90 100
ELNKGLTKKG GNIPMIPGWV MEFPTGKESG NYLAIDLGGT NLRVVLVKLS
110 120 130 140 150
GNHTFDTTQS KYKLPHDMRT TKHQEELWSF IADSLKDFMV EQELLNTKDT
160 170 180 190 200
LPLGFTFSYP ASQNKINEGI LQRWTKGFDI PNVEGHDVVP LLQNEISKRE
210 220 230 240 250
LPIEIVALIN DTVGTLIASY YTDPETKMGV IFGTGVNGAF YDVVSDIEKL
260 270 280 290 300
EGKLADDIPS NSPMAINCEY GSFDNEHLVL PRTKYDVAVD EQSPRPGQQA
310 320 330 340 350
FEKMTSGYYL GELLRLVLLE LNEKGLMLKD QDLSKLKQPY IMDTSYPARI
360 370 380 390 400
EDDPFENLED TDDIFQKDFG VKTTLPERKL IRRLCELIGT RAARLAVCGI
410 420 430 440 450
AAICQKRGYK TGHIAADGSV YNKYPGFKEA AAKGLRDIYG WTGDASKDPI
460 470 480
TIVPAEDGSG AGAAVIAALS EKRIAEGKSL GIIGA
Length:485
Mass (Da):53,738
Last modified:November 1, 1995 - v2
Checksum:iAF5C9DA8F17BC3D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611G → V in CAA27202 (PubMed:3003701).Curated
Sequence conflicti103 – 1031H → R in CAA27202 (PubMed:3003701).Curated
Sequence conflicti194 – 1941N → K in CAA27202 (PubMed:3003701).Curated
Sequence conflicti244 – 2441V → C in CAA27202 (PubMed:3003701).Curated
Sequence conflicti356 – 3572EN → VF in AAA34698 (PubMed:3908224).Curated
Sequence conflicti364 – 3641I → M in CAA27202 (PubMed:3003701).Curated
Sequence conflicti388 – 3881I → T in AAA34698 (PubMed:3908224).Curated
Sequence conflicti444 – 4441D → EN in CAA27202 (PubMed:3003701).Curated
Sequence conflicti479 – 4802SL → VS in CAA27202 (PubMed:3003701).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14410 mRNA. Translation: AAA34698.1.
X03482 Genomic DNA. Translation: CAA27202.1.
D50617 Genomic DNA. Translation: BAA09292.1.
BK006940 Genomic DNA. Translation: DAA12496.1.
PIRiS56308. KIBYHA.
RefSeqiNP_116711.3. NM_001180018.3.

Genome annotation databases

EnsemblFungiiBAA09292; BAA09292; BAA09292.
YFR053C; YFR053C; YFR053C.
GeneIDi850614.
KEGGisce:YFR053C.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14410 mRNA. Translation: AAA34698.1.
X03482 Genomic DNA. Translation: CAA27202.1.
D50617 Genomic DNA. Translation: BAA09292.1.
BK006940 Genomic DNA. Translation: DAA12496.1.
PIRiS56308. KIBYHA.
RefSeqiNP_116711.3. NM_001180018.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HKGX-ray3.50A152-466[»]
3B8AX-ray2.95X1-485[»]
ProteinModelPortaliP04806.
SMRiP04806. Positions 15-485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31211. 58 interactions.
DIPiDIP-5377N.
IntActiP04806. 2 interactions.
MINTiMINT-561285.

Chemistry

BindingDBiP04806.

PTM databases

iPTMnetiP04806.

2D gel databases

SWISS-2DPAGEP04806.

Proteomic databases

MaxQBiP04806.
TopDownProteomicsiP04806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09292; BAA09292; BAA09292.
YFR053C; YFR053C; YFR053C.
GeneIDi850614.
KEGGisce:YFR053C.

Organism-specific databases

EuPathDBiFungiDB:YFR053C.
SGDiS000001949. HXK1.

Phylogenomic databases

GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
InParanoidiP04806.
KOiK00844.
OMAiYIMDTSY.
OrthoDBiEOG092C2JW4.

Enzyme and pathway databases

UniPathwayiUPA00242.
BioCyciYEAST:YFR053C-MONOMER.
BRENDAi2.7.1.1. 984.
ReactomeiR-SCE-170822. Regulation of Glucokinase by Glucokinase Regulatory Protein.
R-SCE-70153. Glucose transport.
R-SCE-70171. Glycolysis.
SABIO-RKP04806.

Miscellaneous databases

EvolutionaryTraceiP04806.
PROiP04806.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHXKA_YEAST
AccessioniPrimary (citable) accession number: P04806
Secondary accession number(s): D6VTT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 177 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In yeast there are three glucose-phosphorylating isoenzymes, designated hexokinase I, II and glucokinase.
Present with 40800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.