SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P04803

- SYWM_YEAST

UniProt

P04803 - SYWM_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Tryptophan--tRNA ligase, mitochondrial
Gene
MSW1, MSW, YDR268W, D9954.7
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei247 – 2471ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. tryptophan-tRNA ligase activity Source: SGD

GO - Biological processi

  1. mitochondrial tryptophanyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29838-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, mitochondrial (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:MSW1
Synonyms:MSW
Ordered Locus Names:YDR268W
ORF Names:D9954.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR268w.
SGDiS000002676. MSW1.

Subcellular locationi

Mitochondrion matrix 3 Publications

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379Tryptophan--tRNA ligase, mitochondrial
PRO_0000136745Add
BLAST

Proteomic databases

MaxQBiP04803.
PaxDbiP04803.
PeptideAtlasiP04803.

Expressioni

Gene expression databases

GenevestigatoriP04803.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-18837,EBI-18837

Protein-protein interaction databases

BioGridi32324. 3 interactions.
DIPiDIP-4584N.
IntActiP04803. 4 interactions.
MINTiMINT-518167.
STRINGi4932.YDR268W.

Structurei

3D structure databases

ProteinModelPortaliP04803.
SMRiP04803. Positions 36-379.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 519"HIGH" region
Motifi244 – 2485"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0180.
GeneTreeiENSGT00510000047425.
HOGENOMiHOG000059940.
KOiK01867.
OMAiSHILHRP.
OrthoDBiEOG7M6DK3.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04803-1 [UniParc]FASTAAdd to Basket

« Hide

MSNKQAVLKL ISKRWISTVQ RADFKLNSEA LHSNATVFSM IQPTGCFHLG    50
NYLGATRVWT DLCELKQPGQ ELIFGVADLH AITVPKPDGE MFRKFRHEAV 100
ASILAVGVDP EKASVIYQSA IPQHSELHWL LSTLASMGLL NRMTQWKSKS 150
NIKQSTNGDY LVNDSDVGKV RLGLFSYPVL QAADILLYKS THVPVGDDQS 200
QHLELTRHLA EKFNKMYKKN FFPKPVTMLA QTKKVLSLST PEKKMSKSDP 250
NHDSVIFLND EPKAIQKKIR KALTDSISDR FYYDPVERPG VSNLINIVSG 300
IQRKSIEDVV EDVSRFNNYR DFKDYVSEVI IEELKGPRTE FEKYINEPTY 350
LHSVVESGMR KAREKAAKNL ADIHKIMGF 379
Length:379
Mass (Da):43,015
Last modified:October 1, 1993 - v2
Checksum:i807B6D21A991E108
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti268 – 2714KIRK → RLE in AAA34809. 1 Publication
Sequence conflicti371 – 3799ADIHKIMGF → PTFIK in AAA34809. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M12081 Genomic DNA. Translation: AAA34809.1.
X66165 Genomic DNA. Translation: CAA46947.1.
U51030 Genomic DNA. Translation: AAB64452.1.
BK006938 Genomic DNA. Translation: DAA12112.1.
PIRiS70128. YWBYM.
RefSeqiNP_010554.1. NM_001180576.1.

Genome annotation databases

EnsemblFungiiYDR268W; YDR268W; YDR268W.
GeneIDi851861.
KEGGisce:YDR268W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M12081 Genomic DNA. Translation: AAA34809.1 .
X66165 Genomic DNA. Translation: CAA46947.1 .
U51030 Genomic DNA. Translation: AAB64452.1 .
BK006938 Genomic DNA. Translation: DAA12112.1 .
PIRi S70128. YWBYM.
RefSeqi NP_010554.1. NM_001180576.1.

3D structure databases

ProteinModelPortali P04803.
SMRi P04803. Positions 36-379.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32324. 3 interactions.
DIPi DIP-4584N.
IntActi P04803. 4 interactions.
MINTi MINT-518167.
STRINGi 4932.YDR268W.

Proteomic databases

MaxQBi P04803.
PaxDbi P04803.
PeptideAtlasi P04803.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR268W ; YDR268W ; YDR268W .
GeneIDi 851861.
KEGGi sce:YDR268W.

Organism-specific databases

CYGDi YDR268w.
SGDi S000002676. MSW1.

Phylogenomic databases

eggNOGi COG0180.
GeneTreei ENSGT00510000047425.
HOGENOMi HOG000059940.
KOi K01867.
OMAi SHILHRP.
OrthoDBi EOG7M6DK3.

Enzyme and pathway databases

BioCyci YEAST:G3O-29838-MONOMER.

Miscellaneous databases

NextBioi 969803.
PROi P04803.

Gene expression databases

Genevestigatori P04803.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
[Graphical view ]
PANTHERi PTHR10055. PTHR10055. 1 hit.
Pfami PF00579. tRNA-synt_1b. 1 hit.
[Graphical view ]
PRINTSi PR01039. TRNASYNTHTRP.
TIGRFAMsi TIGR00233. trpS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "MSW, a yeast gene coding for mitochondrial tryptophanyl-tRNA synthetase."
    Myers A.M., Tzagoloff A.
    J. Biol. Chem. 260:15371-15377(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "An yeast nuclear mutation conferring temperature-sensitivity to the mitochondrial tryptophanyl-tRNA synthetase."
    Entrup R., Langgut W., Lisowsky T., Schweizer E.
    Curr. Genet. 21:281-283(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
    Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
    J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYWM_YEAST
AccessioniPrimary (citable) accession number: P04803
Secondary accession number(s): D6VSQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi