Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tryptophan--tRNA ligase, mitochondrial

Gene

MSW1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42ATPBy similarity1
Binding sitei184L-tryptophanBy similarity1
Binding sitei235ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei247ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 51ATPBy similarity4
Nucleotide bindingi196 – 198ATPBy similarity3
Nucleotide bindingi244 – 248ATPBy similarity5

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • tryptophan-tRNA ligase activity Source: SGD

GO - Biological processi

  • mitochondrial tryptophanyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29838-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, mitochondrial (EC:6.1.1.2)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:MSW1
Synonyms:MSW
Ordered Locus Names:YDR268W
ORF Names:D9954.7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR268W
SGDiS000002676 MSW1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367451 – 379Tryptophan--tRNA ligase, mitochondrialAdd BLAST379

Proteomic databases

MaxQBiP04803
PaxDbiP04803
PRIDEiP04803

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi32324, 14 interactors
DIPiDIP-4584N
IntActiP04803, 6 interactors
STRINGi4932.YDR268W

Structurei

3D structure databases

ProteinModelPortaliP04803
SMRiP04803
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 51"HIGH" region9
Motifi244 – 248"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000047425
HOGENOMiHOG000059940
InParanoidiP04803
KOiK01867
OMAiQRMTQFK
OrthoDBiEOG092C3VLE

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P04803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKQAVLKL ISKRWISTVQ RADFKLNSEA LHSNATVFSM IQPTGCFHLG
60 70 80 90 100
NYLGATRVWT DLCELKQPGQ ELIFGVADLH AITVPKPDGE MFRKFRHEAV
110 120 130 140 150
ASILAVGVDP EKASVIYQSA IPQHSELHWL LSTLASMGLL NRMTQWKSKS
160 170 180 190 200
NIKQSTNGDY LVNDSDVGKV RLGLFSYPVL QAADILLYKS THVPVGDDQS
210 220 230 240 250
QHLELTRHLA EKFNKMYKKN FFPKPVTMLA QTKKVLSLST PEKKMSKSDP
260 270 280 290 300
NHDSVIFLND EPKAIQKKIR KALTDSISDR FYYDPVERPG VSNLINIVSG
310 320 330 340 350
IQRKSIEDVV EDVSRFNNYR DFKDYVSEVI IEELKGPRTE FEKYINEPTY
360 370
LHSVVESGMR KAREKAAKNL ADIHKIMGF
Length:379
Mass (Da):43,015
Last modified:October 1, 1993 - v2
Checksum:i807B6D21A991E108
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti268 – 271KIRK → RLE in AAA34809 (PubMed:2999114).Curated4
Sequence conflicti371 – 379ADIHKIMGF → PTFIK in AAA34809 (PubMed:2999114).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12081 Genomic DNA Translation: AAA34809.1
X66165 Genomic DNA Translation: CAA46947.1
U51030 Genomic DNA Translation: AAB64452.1
BK006938 Genomic DNA Translation: DAA12112.1
PIRiS70128 YWBYM
RefSeqiNP_010554.1, NM_001180576.1

Genome annotation databases

EnsemblFungiiYDR268W; YDR268W; YDR268W
GeneIDi851861
KEGGisce:YDR268W

Similar proteinsi

Entry informationi

Entry nameiSYWM_YEAST
AccessioniPrimary (citable) accession number: P04803
Secondary accession number(s): D6VSQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health