Tryptophan--tRNA ligase, mitochondrial - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P04803 (SYWM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 127. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase, mitochondrial
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	MSW1
Synonyms:	MSW
Ordered Locus Names:	YDR268W
ORF Names:	D9954.7

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).
Subunit structure	Homodimer.
Subcellular location	Mitochondrion matrix Ref.5 Ref.7 Ref.8.
Miscellaneous	Present with 672 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial tryptophanyl-tRNA aminoacylation Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay Ref.7 Ref.8. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan-tRNA ligase activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-18837,EBI-18837

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 379

379

Tryptophan--tRNA ligase, mitochondrial

PRO_0000136745

Regions

Motif

43 – 51

"HIGH" region

Motif

244 – 248

"KMSKS" region

Sites

Binding site

247

ATP By similarity

Experimental info

Sequence conflict

268 – 271

KIRK → RLE in AAA34809. Ref.1

Sequence conflict

371 – 379

ADIHKIMGF → PTFIK in AAA34809. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P04803 [UniParc].

Last modified October 1, 1993. Version 2.
Checksum: 807B6D21A991E108
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FASTA

379

43,015

        10         20         30         40         50         60 
MSNKQAVLKL ISKRWISTVQ RADFKLNSEA LHSNATVFSM IQPTGCFHLG NYLGATRVWT 

        70         80         90        100        110        120 
DLCELKQPGQ ELIFGVADLH AITVPKPDGE MFRKFRHEAV ASILAVGVDP EKASVIYQSA 

       130        140        150        160        170        180 
IPQHSELHWL LSTLASMGLL NRMTQWKSKS NIKQSTNGDY LVNDSDVGKV RLGLFSYPVL 

       190        200        210        220        230        240 
QAADILLYKS THVPVGDDQS QHLELTRHLA EKFNKMYKKN FFPKPVTMLA QTKKVLSLST 

       250        260        270        280        290        300 
PEKKMSKSDP NHDSVIFLND EPKAIQKKIR KALTDSISDR FYYDPVERPG VSNLINIVSG 

       310        320        330        340        350        360 
IQRKSIEDVV EDVSRFNNYR DFKDYVSEVI IEELKGPRTE FEKYINEPTY LHSVVESGMR 

       370 
KAREKAAKNL ADIHKIMGF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MSW, a yeast gene coding for mitochondrial tryptophanyl-tRNA synthetase." Myers A.M., Tzagoloff A. J. Biol. Chem. 260:15371-15377(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"An yeast nuclear mutation conferring temperature-sensitivity to the mitochondrial tryptophanyl-tRNA synthetase." Entrup R., Langgut W., Lisowsky T., Schweizer E. Curr. Genet. 21:281-283(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[8]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12081 Genomic DNA. Translation: AAA34809.1. X66165 Genomic DNA. Translation: CAA46947.1. U51030 Genomic DNA. Translation: AAB64452.1. BK006938 Genomic DNA. Translation: DAA12112.1.
PIR	YWBYM. S70128.
RefSeq	NP_010554.1. NM_001180576.1.
3D structure databases
ProteinModelPortal	P04803.
SMR	P04803. Positions 36-379.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4584N.
IntAct	P04803. 4 interactions.
MINT	MINT-518167.
STRING	4932.YDR268W.
Proteomic databases
PaxDb	P04803.
PeptideAtlas	P04803.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR268W; YDR268W; YDR268W.
GeneID	851861.
KEGG	sce:YDR268W.
Organism-specific databases
CYGD	YDR268w.
SGD	S000002676. MSW1.
Phylogenomic databases
eggNOG	COG0180.
GeneTree	ENSGT00510000047425.
HOGENOM	HOG000059940.
KO	K01867.
OMA	QWKSKAQ.
OrthoDB	EOG42VCRK.
Gene expression databases
Genevestigator	P04803.
GermOnline	YDR268W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969803.

Entry information

Entry name

SYWM_YEAST

Accession

Primary (citable) accession number: P04803
Secondary accession number(s): D6VSQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents