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P04803

- SYWM_YEAST

UniProt

P04803 - SYWM_YEAST

Protein

Tryptophan--tRNA ligase, mitochondrial

Gene

MSW1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei247 – 2471ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. tryptophan-tRNA ligase activity Source: SGD

    GO - Biological processi

    1. mitochondrial tryptophanyl-tRNA aminoacylation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29838-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tryptophan--tRNA ligase, mitochondrial (EC:6.1.1.2)
    Alternative name(s):
    Tryptophanyl-tRNA synthetase
    Short name:
    TrpRS
    Gene namesi
    Name:MSW1
    Synonyms:MSW
    Ordered Locus Names:YDR268W
    ORF Names:D9954.7
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR268w.
    SGDiS000002676. MSW1.

    Subcellular locationi

    Mitochondrion matrix 3 Publications

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 379379Tryptophan--tRNA ligase, mitochondrialPRO_0000136745Add
    BLAST

    Proteomic databases

    MaxQBiP04803.
    PaxDbiP04803.
    PeptideAtlasiP04803.

    Expressioni

    Gene expression databases

    GenevestigatoriP04803.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-18837,EBI-18837

    Protein-protein interaction databases

    BioGridi32324. 3 interactions.
    DIPiDIP-4584N.
    IntActiP04803. 4 interactions.
    MINTiMINT-518167.
    STRINGi4932.YDR268W.

    Structurei

    3D structure databases

    ProteinModelPortaliP04803.
    SMRiP04803. Positions 36-379.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi43 – 519"HIGH" region
    Motifi244 – 2485"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0180.
    GeneTreeiENSGT00510000047425.
    HOGENOMiHOG000059940.
    KOiK01867.
    OMAiSHILHRP.
    OrthoDBiEOG7M6DK3.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002305. aa-tRNA-synth_Ic.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002306. Trp-tRNA-ligase.
    [Graphical view]
    PANTHERiPTHR10055. PTHR10055. 1 hit.
    PfamiPF00579. tRNA-synt_1b. 1 hit.
    [Graphical view]
    PRINTSiPR01039. TRNASYNTHTRP.
    TIGRFAMsiTIGR00233. trpS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04803-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNKQAVLKL ISKRWISTVQ RADFKLNSEA LHSNATVFSM IQPTGCFHLG    50
    NYLGATRVWT DLCELKQPGQ ELIFGVADLH AITVPKPDGE MFRKFRHEAV 100
    ASILAVGVDP EKASVIYQSA IPQHSELHWL LSTLASMGLL NRMTQWKSKS 150
    NIKQSTNGDY LVNDSDVGKV RLGLFSYPVL QAADILLYKS THVPVGDDQS 200
    QHLELTRHLA EKFNKMYKKN FFPKPVTMLA QTKKVLSLST PEKKMSKSDP 250
    NHDSVIFLND EPKAIQKKIR KALTDSISDR FYYDPVERPG VSNLINIVSG 300
    IQRKSIEDVV EDVSRFNNYR DFKDYVSEVI IEELKGPRTE FEKYINEPTY 350
    LHSVVESGMR KAREKAAKNL ADIHKIMGF 379
    Length:379
    Mass (Da):43,015
    Last modified:October 1, 1993 - v2
    Checksum:i807B6D21A991E108
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti268 – 2714KIRK → RLE in AAA34809. (PubMed:2999114)Curated
    Sequence conflicti371 – 3799ADIHKIMGF → PTFIK in AAA34809. (PubMed:2999114)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12081 Genomic DNA. Translation: AAA34809.1.
    X66165 Genomic DNA. Translation: CAA46947.1.
    U51030 Genomic DNA. Translation: AAB64452.1.
    BK006938 Genomic DNA. Translation: DAA12112.1.
    PIRiS70128. YWBYM.
    RefSeqiNP_010554.1. NM_001180576.1.

    Genome annotation databases

    EnsemblFungiiYDR268W; YDR268W; YDR268W.
    GeneIDi851861.
    KEGGisce:YDR268W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12081 Genomic DNA. Translation: AAA34809.1 .
    X66165 Genomic DNA. Translation: CAA46947.1 .
    U51030 Genomic DNA. Translation: AAB64452.1 .
    BK006938 Genomic DNA. Translation: DAA12112.1 .
    PIRi S70128. YWBYM.
    RefSeqi NP_010554.1. NM_001180576.1.

    3D structure databases

    ProteinModelPortali P04803.
    SMRi P04803. Positions 36-379.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32324. 3 interactions.
    DIPi DIP-4584N.
    IntActi P04803. 4 interactions.
    MINTi MINT-518167.
    STRINGi 4932.YDR268W.

    Proteomic databases

    MaxQBi P04803.
    PaxDbi P04803.
    PeptideAtlasi P04803.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR268W ; YDR268W ; YDR268W .
    GeneIDi 851861.
    KEGGi sce:YDR268W.

    Organism-specific databases

    CYGDi YDR268w.
    SGDi S000002676. MSW1.

    Phylogenomic databases

    eggNOGi COG0180.
    GeneTreei ENSGT00510000047425.
    HOGENOMi HOG000059940.
    KOi K01867.
    OMAi SHILHRP.
    OrthoDBi EOG7M6DK3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29838-MONOMER.

    Miscellaneous databases

    NextBioi 969803.
    PROi P04803.

    Gene expression databases

    Genevestigatori P04803.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002305. aa-tRNA-synth_Ic.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002306. Trp-tRNA-ligase.
    [Graphical view ]
    PANTHERi PTHR10055. PTHR10055. 1 hit.
    Pfami PF00579. tRNA-synt_1b. 1 hit.
    [Graphical view ]
    PRINTSi PR01039. TRNASYNTHTRP.
    TIGRFAMsi TIGR00233. trpS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "MSW, a yeast gene coding for mitochondrial tryptophanyl-tRNA synthetase."
      Myers A.M., Tzagoloff A.
      J. Biol. Chem. 260:15371-15377(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "An yeast nuclear mutation conferring temperature-sensitivity to the mitochondrial tryptophanyl-tRNA synthetase."
      Entrup R., Langgut W., Lisowsky T., Schweizer E.
      Curr. Genet. 21:281-283(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    8. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
      Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
      J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYWM_YEAST
    AccessioniPrimary (citable) accession number: P04803
    Secondary accession number(s): D6VSQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 672 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3