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Protein

Aspartate--tRNA ligase, cytoplasmic

Gene

DPS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei281AspartateBy similarity1
Binding sitei325AspartateBy similarity1
Binding sitei478ATPBy similarity1
Binding sitei481AspartateBy similarity1
Binding sitei485AspartateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi325 – 327ATPBy similarity3
Nucleotide bindingi333 – 335ATPBy similarity3
Nucleotide bindingi528 – 531ATPBy similarity4

GO - Molecular functioni

  • aspartate-tRNA ligase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • RNA binding Source: SGD

GO - Biological processi

  • aspartyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32123-MONOMER.
BRENDAi6.1.1.12. 984.
SABIO-RKP04802.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligase, cytoplasmic (EC:6.1.1.12)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Gene namesi
Name:DPS1
Synonyms:APS, APS1
Ordered Locus Names:YLL018C
ORF Names:L1295
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL018C.
SGDiS000003941. DPS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi273P → G: Loss of activity; important for dimerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001110142 – 557Aspartate--tRNA ligase, cytoplasmicAdd BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei14PhosphoserineCombined sources1
Modified residuei301PhosphoserineCombined sources1
Modified residuei502PhosphoserineCombined sources1
Modified residuei546PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP04802.
PRIDEiP04802.

PTM databases

iPTMnetiP04802.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi31235. 59 interactors.
DIPiDIP-4093N.
IntActiP04802. 15 interactors.
MINTiMINT-510718.

Structurei

Secondary structure

1557
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi73 – 77Combined sources5
Helixi85 – 87Combined sources3
Helixi96 – 98Combined sources3
Turni101 – 106Combined sources6
Beta strandi108 – 120Combined sources13
Beta strandi122 – 132Combined sources11
Beta strandi135 – 142Combined sources8
Beta strandi145 – 149Combined sources5
Helixi151 – 157Combined sources7
Beta strandi165 – 174Combined sources10
Beta strandi182 – 198Combined sources17
Helixi208 – 211Combined sources4
Helixi215 – 220Combined sources6
Helixi228 – 233Combined sources6
Helixi235 – 238Combined sources4
Helixi242 – 264Combined sources23
Beta strandi274 – 278Combined sources5
Beta strandi280 – 284Combined sources5
Beta strandi288 – 291Combined sources4
Beta strandi294 – 298Combined sources5
Helixi303 – 311Combined sources9
Beta strandi316 – 324Combined sources9
Beta strandi331 – 333Combined sources3
Beta strandi336 – 346Combined sources11
Helixi352 – 372Combined sources21
Helixi374 – 383Combined sources10
Beta strandi393 – 395Combined sources3
Beta strandi398 – 401Combined sources4
Helixi402 – 411Combined sources10
Beta strandi418 – 420Combined sources3
Helixi424 – 437Combined sources14
Beta strandi441 – 446Combined sources6
Helixi450 – 452Combined sources3
Beta strandi461 – 463Combined sources3
Beta strandi466 – 474Combined sources9
Beta strandi477 – 485Combined sources9
Helixi489 – 498Combined sources10
Turni506 – 508Combined sources3
Helixi509 – 515Combined sources7
Beta strandi522 – 528Combined sources7
Helixi529 – 536Combined sources8
Helixi542 – 545Combined sources4
Beta strandi546 – 548Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ASYX-ray2.90A/B68-557[»]
1ASZX-ray3.00A/B68-557[»]
1EOVX-ray2.30A71-557[»]
ProteinModelPortaliP04802.
SMRiP04802.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04802.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni303 – 306AspartateBy similarity4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074880.
HOGENOMiHOG000226032.
InParanoidiP04802.
KOiK01876.
OMAiWVWEIRD.
OrthoDBiEOG092C04LM.

Family and domain databases

HAMAPiMF_02075. Asp_tRNA_synth_type2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_arc.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF33. PTHR22594:SF33. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQDENIVKA VEESAEPAQV ILGEDGKPLS KKALKKLQKE QEKQRKKEER
60 70 80 90 100
ALQLEAEREA REKKAAAEDT AKDNYGKLPL IQSRDSDRTG QKRVKFVDLD
110 120 130 140 150
EAKDSDKEVL FRARVHNTRQ QGATLAFLTL RQQASLIQGL VKANKEGTIS
160 170 180 190 200
KNMVKWAGSL NLESIVLVRG IVKKVDEPIK SATVQNLEIH ITKIYTISET
210 220 230 240 250
PEALPILLED ASRSEAEAEA AGLPVVNLDT RLDYRVIDLR TVTNQAIFRI
260 270 280 290 300
QAGVCELFRE YLATKKFTEV HTPKLLGAPS EGGSSVFEVT YFKGKAYLAQ
310 320 330 340 350
SPQFNKQQLI VADFERVYEI GPVFRAENSN THRHMTEFTG LDMEMAFEEH
360 370 380 390 400
YHEVLDTLSE LFVFIFSELP KRFAHEIELV RKQYPVEEFK LPKDGKMVRL
410 420 430 440 450
TYKEGIEMLR AAGKEIGDFE DLSTENEKFL GKLVRDKYDT DFYILDKFPL
460 470 480 490 500
EIRPFYTMPD PANPKYSNSY DFFMRGEEIL SGAQRIHDHA LLQERMKAHG
510 520 530 540 550
LSPEDPGLKD YCDGFSYGCP PHAGGGIGLE RVVMFYLDLK NIRRASLFPR

DPKRLRP
Length:557
Mass (Da):63,516
Last modified:January 23, 2007 - v3
Checksum:i6656279B3E9011A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03606 Genomic DNA. Translation: CAA27269.1.
X06665 Genomic DNA. Translation: CAA29865.1.
X97560 Genomic DNA. Translation: CAA66172.1.
Z73123 Genomic DNA. Translation: CAA97464.1.
Z73122 Genomic DNA. Translation: CAA97463.1.
X91488 Genomic DNA. Translation: CAA62772.1.
BK006945 Genomic DNA. Translation: DAA09302.1.
PIRiA23508. SYBYDC.
RefSeqiNP_013083.1. NM_001181838.1.

Genome annotation databases

EnsemblFungiiYLL018C; YLL018C; YLL018C.
GeneIDi850643.
KEGGisce:YLL018C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03606 Genomic DNA. Translation: CAA27269.1.
X06665 Genomic DNA. Translation: CAA29865.1.
X97560 Genomic DNA. Translation: CAA66172.1.
Z73123 Genomic DNA. Translation: CAA97464.1.
Z73122 Genomic DNA. Translation: CAA97463.1.
X91488 Genomic DNA. Translation: CAA62772.1.
BK006945 Genomic DNA. Translation: DAA09302.1.
PIRiA23508. SYBYDC.
RefSeqiNP_013083.1. NM_001181838.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ASYX-ray2.90A/B68-557[»]
1ASZX-ray3.00A/B68-557[»]
1EOVX-ray2.30A71-557[»]
ProteinModelPortaliP04802.
SMRiP04802.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31235. 59 interactors.
DIPiDIP-4093N.
IntActiP04802. 15 interactors.
MINTiMINT-510718.

PTM databases

iPTMnetiP04802.

Proteomic databases

MaxQBiP04802.
PRIDEiP04802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL018C; YLL018C; YLL018C.
GeneIDi850643.
KEGGisce:YLL018C.

Organism-specific databases

EuPathDBiFungiDB:YLL018C.
SGDiS000003941. DPS1.

Phylogenomic databases

GeneTreeiENSGT00550000074880.
HOGENOMiHOG000226032.
InParanoidiP04802.
KOiK01876.
OMAiWVWEIRD.
OrthoDBiEOG092C04LM.

Enzyme and pathway databases

BioCyciYEAST:G3O-32123-MONOMER.
BRENDAi6.1.1.12. 984.
SABIO-RKP04802.

Miscellaneous databases

EvolutionaryTraceiP04802.
PROiP04802.

Family and domain databases

HAMAPiMF_02075. Asp_tRNA_synth_type2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_arc.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF33. PTHR22594:SF33. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYDC_YEAST
AccessioniPrimary (citable) accession number: P04802
Secondary accession number(s): D6VXY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 184 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5750 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.