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Protein

Aspartate--tRNA ligase, cytoplasmic

Gene

DPS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 5750 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei281AspartateBy similarity1
Binding sitei325AspartateBy similarity1
Binding sitei478ATPBy similarity1
Binding sitei481AspartateBy similarity1
Binding sitei485AspartateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi325 – 327ATPBy similarity3
Nucleotide bindingi333 – 335ATPBy similarity3
Nucleotide bindingi528 – 531ATPBy similarity4

GO - Molecular functioni

  • aspartate-tRNA ligase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • RNA binding Source: SGD

GO - Biological processi

  • aspartyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32123-MONOMER
BRENDAi6.1.1.12 984
SABIO-RKiP04802

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligase, cytoplasmic (EC:6.1.1.12)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Gene namesi
Name:DPS1
Synonyms:APS, APS1
Ordered Locus Names:YLL018C
ORF Names:L1295
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL018C
SGDiS000003941 DPS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi273P → G: Loss of activity; important for dimerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001110142 – 557Aspartate--tRNA ligase, cytoplasmicAdd BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei14PhosphoserineCombined sources1
Modified residuei301PhosphoserineCombined sources1
Modified residuei502PhosphoserineCombined sources1
Modified residuei546PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP04802
PaxDbiP04802
PRIDEiP04802

PTM databases

CarbonylDBiP04802
iPTMnetiP04802

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
SAW1P397352EBI-18655,EBI-20627

Protein-protein interaction databases

BioGridi31235, 64 interactors
DIPiDIP-4093N
IntActiP04802, 23 interactors
MINTiP04802
STRINGi4932.YLL018C

Structurei

Secondary structure

1557
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi73 – 77Combined sources5
Helixi85 – 87Combined sources3
Helixi96 – 98Combined sources3
Turni101 – 106Combined sources6
Beta strandi108 – 120Combined sources13
Beta strandi122 – 132Combined sources11
Beta strandi135 – 142Combined sources8
Beta strandi145 – 149Combined sources5
Helixi151 – 157Combined sources7
Beta strandi165 – 174Combined sources10
Beta strandi182 – 198Combined sources17
Helixi208 – 211Combined sources4
Helixi215 – 220Combined sources6
Helixi228 – 233Combined sources6
Helixi235 – 238Combined sources4
Helixi242 – 264Combined sources23
Beta strandi274 – 278Combined sources5
Beta strandi280 – 284Combined sources5
Beta strandi288 – 291Combined sources4
Beta strandi294 – 298Combined sources5
Helixi303 – 311Combined sources9
Beta strandi316 – 324Combined sources9
Beta strandi331 – 333Combined sources3
Beta strandi336 – 346Combined sources11
Helixi352 – 372Combined sources21
Helixi374 – 383Combined sources10
Beta strandi393 – 395Combined sources3
Beta strandi398 – 401Combined sources4
Helixi402 – 411Combined sources10
Beta strandi418 – 420Combined sources3
Helixi424 – 437Combined sources14
Beta strandi441 – 446Combined sources6
Helixi450 – 452Combined sources3
Beta strandi461 – 463Combined sources3
Beta strandi466 – 474Combined sources9
Beta strandi477 – 485Combined sources9
Helixi489 – 498Combined sources10
Turni506 – 508Combined sources3
Helixi509 – 515Combined sources7
Beta strandi522 – 528Combined sources7
Helixi529 – 536Combined sources8
Helixi542 – 545Combined sources4
Beta strandi546 – 548Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ASYX-ray2.90A/B68-557[»]
1ASZX-ray3.00A/B68-557[»]
1EOVX-ray2.30A71-557[»]
ProteinModelPortaliP04802
SMRiP04802
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04802

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni303 – 306AspartateBy similarity4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074880
HOGENOMiHOG000226032
InParanoidiP04802
KOiK01876
OMAiWVHEIRD
OrthoDBiEOG092C04LM

Family and domain databases

HAMAPiMF_02075 Asp_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR004523 Asp-tRNA_synthase_2
IPR002312 Asp/Asn-tRNA-synth_IIb
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PANTHERiPTHR43450 PTHR43450, 1 hit
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR01042 TRNASYNTHASP
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00458 aspS_nondisc, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQDENIVKA VEESAEPAQV ILGEDGKPLS KKALKKLQKE QEKQRKKEER
60 70 80 90 100
ALQLEAEREA REKKAAAEDT AKDNYGKLPL IQSRDSDRTG QKRVKFVDLD
110 120 130 140 150
EAKDSDKEVL FRARVHNTRQ QGATLAFLTL RQQASLIQGL VKANKEGTIS
160 170 180 190 200
KNMVKWAGSL NLESIVLVRG IVKKVDEPIK SATVQNLEIH ITKIYTISET
210 220 230 240 250
PEALPILLED ASRSEAEAEA AGLPVVNLDT RLDYRVIDLR TVTNQAIFRI
260 270 280 290 300
QAGVCELFRE YLATKKFTEV HTPKLLGAPS EGGSSVFEVT YFKGKAYLAQ
310 320 330 340 350
SPQFNKQQLI VADFERVYEI GPVFRAENSN THRHMTEFTG LDMEMAFEEH
360 370 380 390 400
YHEVLDTLSE LFVFIFSELP KRFAHEIELV RKQYPVEEFK LPKDGKMVRL
410 420 430 440 450
TYKEGIEMLR AAGKEIGDFE DLSTENEKFL GKLVRDKYDT DFYILDKFPL
460 470 480 490 500
EIRPFYTMPD PANPKYSNSY DFFMRGEEIL SGAQRIHDHA LLQERMKAHG
510 520 530 540 550
LSPEDPGLKD YCDGFSYGCP PHAGGGIGLE RVVMFYLDLK NIRRASLFPR

DPKRLRP
Length:557
Mass (Da):63,516
Last modified:January 23, 2007 - v3
Checksum:i6656279B3E9011A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03606 Genomic DNA Translation: CAA27269.1
X06665 Genomic DNA Translation: CAA29865.1
X97560 Genomic DNA Translation: CAA66172.1
Z73123 Genomic DNA Translation: CAA97464.1
Z73122 Genomic DNA Translation: CAA97463.1
X91488 Genomic DNA Translation: CAA62772.1
BK006945 Genomic DNA Translation: DAA09302.1
PIRiA23508 SYBYDC
RefSeqiNP_013083.1, NM_001181838.1

Genome annotation databases

EnsemblFungiiYLL018C; YLL018C; YLL018C
GeneIDi850643
KEGGisce:YLL018C

Similar proteinsi

Entry informationi

Entry nameiSYDC_YEAST
AccessioniPrimary (citable) accession number: P04802
Secondary accession number(s): D6VXY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 196 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health