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Protein

Threonine--tRNA ligase, cytoplasmic

Gene

THS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 42600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • threonine-tRNA ligase activity Source: SGD

GO - Biological processi

  • threonyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31343-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase, cytoplasmic (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Gene namesi
Name:THS1
Ordered Locus Names:YIL078W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL078W
SGDiS000001340 THS1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001011251 – 734Threonine--tRNA ligase, cytoplasmicAdd BLAST734

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei195PhosphoserineCombined sources1
Modified residuei289PhosphoserineCombined sources1
Modified residuei297PhosphothreonineCombined sources1
Modified residuei381PhosphothreonineCombined sources1
Modified residuei453PhosphoserineCombined sources1
Modified residuei457PhosphoserineCombined sources1
Modified residuei460PhosphothreonineCombined sources1
Modified residuei605PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04801
PaxDbiP04801
PRIDEiP04801

PTM databases

iPTMnetiP04801

Interactioni

Protein-protein interaction databases

BioGridi34914, 279 interactors
DIPiDIP-5307N
IntActiP04801, 17 interactors
MINTiP04801
STRINGi4932.YIL078W

Structurei

3D structure databases

ProteinModelPortaliP04801
SMRiP04801
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000002149
HOGENOMiHOG000003878
InParanoidiP04801
KOiK01868
OMAiFYYDFAY
OrthoDBiEOG092C32CL

Family and domain databases

CDDicd00771 ThrRS_core, 1 hit
Gene3Di3.10.20.30, 1 hit
3.40.50.800, 1 hit
HAMAPiMF_00184 Thr_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR012675 Beta-grasp_dom_sf
IPR004095 TGS
IPR012676 TGS-like
IPR002320 Thr-tRNA-ligase_IIa
IPR018163 Thr/Ala-tRNA-synth_IIc_edit
IPR033728 ThrRS_core
IPR012947 tRNA_SAD
PANTHERiPTHR11451 PTHR11451, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF02824 TGS, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF07973 tRNA_SAD, 1 hit
PRINTSiPR01047 TRNASYNTHTHR
SMARTiView protein in SMART
SM00863 tRNA_SAD, 1 hit
SUPFAMiSSF55186 SSF55186, 1 hit
SSF81271 SSF81271, 1 hit
TIGRFAMsiTIGR00418 thrS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

P04801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASEAGVTE QVKKLSVKDS SNDAVKPNKK ENKKSKQQSL YLDPEPTFIE
60 70 80 90 100
ERIEMFDRLQ KEYNDKVASM PRVPLKIVLK DGAVKEATSW ETTPMDIAKG
110 120 130 140 150
ISKSLADRLC ISKVNGQLWD LDRPFEGEAN EEIKLELLDF ESDEGKKVFW
160 170 180 190 200
HSSAHVLGES CECHLGAHIC LGPPTDDGFF YEMAVRDSMK DISESPERTV
210 220 230 240 250
SQADFPGLEG VAKNVIKQKQ KFERLVMSKE DLLKMFHYSK YKTYLVQTKV
260 270 280 290 300
PDGGATTVYR CGKLIDLCVG PHIPHTGRIK AFKLLKNSSC YFLGDATNDS
310 320 330 340 350
LQRVYGISFP DKKLMDAHLK FLAEASMRDH RKIGKEQELF LFNEMSPGSC
360 370 380 390 400
FWLPHGTRIY NTLVDLLRTE YRKRGYEEVI TPNMYNSKLW ETSGHWANYK
410 420 430 440 450
ENMFTFEVEK ETFGLKPMNC PGHCLMFKSR ERSYRELPWR VADFGVIHRN
460 470 480 490 500
EFSGALSGLT RVRRFQQDDA HIFCTHDQIE SEIENIFNFL QYIYGVFGFE
510 520 530 540 550
FKMELSTRPE KYVGKIETWD AAESKLESAL KKWGGNWEIN AGDGAFYGPK
560 570 580 590 600
IDIMISDALR RWHQCATIQL DFQLPNRFEL EFKSKDQDSE SYERPVMIHR
610 620 630 640 650
AILGSVERMT AILTEHFAGK WPFWLSPRQV LVVPVGVKYQ GYAEDVRNKL
660 670 680 690 700
HDAGFYADVD LTGNTLQKKV RNGQMLKYNF IFIVGEQEMN EKSVNIRNRD
710 720 730
VMEQQGKNAT VSVEEVLKQL RNLKDEKRGD NVLA
Length:734
Mass (Da):84,520
Last modified:February 1, 1995 - v2
Checksum:iEA328F8EC08417C3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9T → A in CAA26666 (PubMed:2995918).Curated1
Sequence conflicti18K → N in CAA26666 (PubMed:2995918).Curated1
Sequence conflicti502K → E in CAA26666 (PubMed:2995918).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37997 Genomic DNA Translation: CAA86092.1
X02906 Genomic DNA Translation: CAA26666.1
BK006942 Genomic DNA Translation: DAA08472.1
PIRiS48366 YSBYTC
RefSeqiNP_116578.3, NM_001179428.3

Genome annotation databases

EnsemblFungiiYIL078W; YIL078W; YIL078W
GeneIDi854732
KEGGisce:YIL078W

Similar proteinsi

Entry informationi

Entry nameiSYTC_YEAST
AccessioniPrimary (citable) accession number: P04801
Secondary accession number(s): D6VVK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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