Threonyl-tRNA synthetase, cytoplasmic - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P04801 (SYTC_YEAST)

Last modified November 3, 2009. Version 97. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	THS1
Ordered Locus Names:	YIL078W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
Subcellular location	Cytoplasm.
Miscellaneous	Present with 42600 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

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Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from direct assay. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct threonine-tRNA ligase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
PGM2	P37012	1	EBI-18814,EBI-13296
PRP40	P33203	1	EBI-18814,EBI-701
SNC2	P33328	1	EBI-18814,EBI-17512
YRF1-4	O13559	1	EBI-18814,EBI-29562

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 734

734

Threonyl-tRNA synthetase, cytoplasmic

PRO_0000101125

Amino acid modifications

Modified residue

195

Phosphoserine Ref.4

Modified residue

199

Phosphothreonine Ref.4

Modified residue

201

Phosphoserine Ref.4

Modified residue

289

Phosphoserine Ref.4

Modified residue

291

Phosphotyrosine Ref.4

Modified residue

381

Phosphothreonine Ref.4

Modified residue

453

Phosphoserine Ref.4

Modified residue

457

Phosphoserine Ref.4

Modified residue

460

Phosphothreonine Ref.4

Modified residue

605

Phosphoserine Ref.4

Experimental info

Sequence conflict

T → A in CAA26666. Ref.1

Sequence conflict

K → N in CAA26666. Ref.1

Sequence conflict

502

K → E in CAA26666. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P04801-1 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: EA328F8EC08417C3
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FASTA

734

84,520

        10         20         30         40         50         60 
MSASEAGVTE QVKKLSVKDS SNDAVKPNKK ENKKSKQQSL YLDPEPTFIE ERIEMFDRLQ 

        70         80         90        100        110        120 
KEYNDKVASM PRVPLKIVLK DGAVKEATSW ETTPMDIAKG ISKSLADRLC ISKVNGQLWD 

       130        140        150        160        170        180 
LDRPFEGEAN EEIKLELLDF ESDEGKKVFW HSSAHVLGES CECHLGAHIC LGPPTDDGFF 

       190        200        210        220        230        240 
YEMAVRDSMK DISESPERTV SQADFPGLEG VAKNVIKQKQ KFERLVMSKE DLLKMFHYSK 

       250        260        270        280        290        300 
YKTYLVQTKV PDGGATTVYR CGKLIDLCVG PHIPHTGRIK AFKLLKNSSC YFLGDATNDS 

       310        320        330        340        350        360 
LQRVYGISFP DKKLMDAHLK FLAEASMRDH RKIGKEQELF LFNEMSPGSC FWLPHGTRIY 

       370        380        390        400        410        420 
NTLVDLLRTE YRKRGYEEVI TPNMYNSKLW ETSGHWANYK ENMFTFEVEK ETFGLKPMNC 

       430        440        450        460        470        480 
PGHCLMFKSR ERSYRELPWR VADFGVIHRN EFSGALSGLT RVRRFQQDDA HIFCTHDQIE 

       490        500        510        520        530        540 
SEIENIFNFL QYIYGVFGFE FKMELSTRPE KYVGKIETWD AAESKLESAL KKWGGNWEIN 

       550        560        570        580        590        600 
AGDGAFYGPK IDIMISDALR RWHQCATIQL DFQLPNRFEL EFKSKDQDSE SYERPVMIHR 

       610        620        630        640        650        660 
AILGSVERMT AILTEHFAGK WPFWLSPRQV LVVPVGVKYQ GYAEDVRNKL HDAGFYADVD 

       670        680        690        700        710        720 
LTGNTLQKKV RNGQMLKYNF IFIVGEQEMN EKSVNIRNRD VMEQQGKNAT VSVEEVLKQL 

       730 
RNLKDEKRGD NVLA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the gene for the yeast cytoplasmic threonyl-tRNA synthetase." Pape L.K., Tzagoloff A. Nucleic Acids Res. 13:6171-6183(1985) [PubMed: 2995918] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195; THR-199; SER-201; SER-289; TYR-291; THR-381; SER-453; SER-457; THR-460 AND SER-605, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	Z37997 Genomic DNA. Translation: CAA86092.1. X02906 Genomic DNA. Translation: CAA26666.1.
PIR	YSBYTC. S48366.
RefSeq	NP_116578.1.
3D structure databases
HSSP	HSSP built from PDB template 1EVL based on UniProtKB P00955.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5307N.
IntAct	P04801. 26 interactions.
STRING	P04801.
Proteomic databases
PeptideAtlas	P04801.
Genome annotation databases
Ensembl	YIL078W; YIL078W; YIL078W; Saccharomyces cerevisiae. [Genome view]
GeneID	854732.
GenomeReviews	Gene locus YIL078W in contig Z47047_GR.
KEGG	sce:YIL078W.
NMPDR	fig\|4932.3.peg.1714.
Organism-specific databases
CYGD	YIL078w.
SGD	S000001340. THS1.
Phylogenomic databases
HOGENOM	P04801.
OMA	IENDCEL.
Enzyme and pathway databases
BRENDA	6.1.1.3. 250.
Gene expression databases
ArrayExpress	P04801.
Genevestigator	P04801.
GermOnline	YIL078W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977428.

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Entry information

Entry name

SYTC_YEAST

Accession

Primary (citable) accession number: P04801

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents