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Protein

Threonine--tRNA ligase, cytoplasmic

Gene

THS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • RNA binding Source: GO_Central
  • threonine-tRNA ligase activity Source: SGD

GO - Biological processi

  • threonyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31343-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase, cytoplasmic (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Gene namesi
Name:THS1
Ordered Locus Names:YIL078W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL078W.
SGDiS000001340. THS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 734734Threonine--tRNA ligase, cytoplasmicPRO_0000101125Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei195 – 1951PhosphoserineCombined sources
Modified residuei289 – 2891PhosphoserineCombined sources
Modified residuei297 – 2971PhosphothreonineCombined sources
Modified residuei381 – 3811PhosphothreonineCombined sources
Modified residuei453 – 4531PhosphoserineCombined sources
Modified residuei457 – 4571PhosphoserineCombined sources
Modified residuei460 – 4601PhosphothreonineCombined sources
Modified residuei605 – 6051PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04801.
PRIDEiP04801.

PTM databases

iPTMnetiP04801.

Interactioni

Protein-protein interaction databases

BioGridi34914. 67 interactions.
DIPiDIP-5307N.
IntActiP04801. 15 interactions.
MINTiMINT-540497.

Structurei

3D structure databases

ProteinModelPortaliP04801.
SMRiP04801. Positions 77-727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000002149.
HOGENOMiHOG000003878.
InParanoidiP04801.
KOiK01868.
OMAiDMANMEG.
OrthoDBiEOG7KDFK9.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASEAGVTE QVKKLSVKDS SNDAVKPNKK ENKKSKQQSL YLDPEPTFIE
60 70 80 90 100
ERIEMFDRLQ KEYNDKVASM PRVPLKIVLK DGAVKEATSW ETTPMDIAKG
110 120 130 140 150
ISKSLADRLC ISKVNGQLWD LDRPFEGEAN EEIKLELLDF ESDEGKKVFW
160 170 180 190 200
HSSAHVLGES CECHLGAHIC LGPPTDDGFF YEMAVRDSMK DISESPERTV
210 220 230 240 250
SQADFPGLEG VAKNVIKQKQ KFERLVMSKE DLLKMFHYSK YKTYLVQTKV
260 270 280 290 300
PDGGATTVYR CGKLIDLCVG PHIPHTGRIK AFKLLKNSSC YFLGDATNDS
310 320 330 340 350
LQRVYGISFP DKKLMDAHLK FLAEASMRDH RKIGKEQELF LFNEMSPGSC
360 370 380 390 400
FWLPHGTRIY NTLVDLLRTE YRKRGYEEVI TPNMYNSKLW ETSGHWANYK
410 420 430 440 450
ENMFTFEVEK ETFGLKPMNC PGHCLMFKSR ERSYRELPWR VADFGVIHRN
460 470 480 490 500
EFSGALSGLT RVRRFQQDDA HIFCTHDQIE SEIENIFNFL QYIYGVFGFE
510 520 530 540 550
FKMELSTRPE KYVGKIETWD AAESKLESAL KKWGGNWEIN AGDGAFYGPK
560 570 580 590 600
IDIMISDALR RWHQCATIQL DFQLPNRFEL EFKSKDQDSE SYERPVMIHR
610 620 630 640 650
AILGSVERMT AILTEHFAGK WPFWLSPRQV LVVPVGVKYQ GYAEDVRNKL
660 670 680 690 700
HDAGFYADVD LTGNTLQKKV RNGQMLKYNF IFIVGEQEMN EKSVNIRNRD
710 720 730
VMEQQGKNAT VSVEEVLKQL RNLKDEKRGD NVLA
Length:734
Mass (Da):84,520
Last modified:February 1, 1995 - v2
Checksum:iEA328F8EC08417C3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91T → A in CAA26666 (PubMed:2995918).Curated
Sequence conflicti18 – 181K → N in CAA26666 (PubMed:2995918).Curated
Sequence conflicti502 – 5021K → E in CAA26666 (PubMed:2995918).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37997 Genomic DNA. Translation: CAA86092.1.
X02906 Genomic DNA. Translation: CAA26666.1.
BK006942 Genomic DNA. Translation: DAA08472.1.
PIRiS48366. YSBYTC.
RefSeqiNP_116578.3. NM_001179428.3.

Genome annotation databases

EnsemblFungiiYIL078W; YIL078W; YIL078W.
GeneIDi854732.
KEGGisce:YIL078W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37997 Genomic DNA. Translation: CAA86092.1.
X02906 Genomic DNA. Translation: CAA26666.1.
BK006942 Genomic DNA. Translation: DAA08472.1.
PIRiS48366. YSBYTC.
RefSeqiNP_116578.3. NM_001179428.3.

3D structure databases

ProteinModelPortaliP04801.
SMRiP04801. Positions 77-727.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34914. 67 interactions.
DIPiDIP-5307N.
IntActiP04801. 15 interactions.
MINTiMINT-540497.

PTM databases

iPTMnetiP04801.

Proteomic databases

MaxQBiP04801.
PRIDEiP04801.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL078W; YIL078W; YIL078W.
GeneIDi854732.
KEGGisce:YIL078W.

Organism-specific databases

EuPathDBiFungiDB:YIL078W.
SGDiS000001340. THS1.

Phylogenomic databases

GeneTreeiENSGT00390000002149.
HOGENOMiHOG000003878.
InParanoidiP04801.
KOiK01868.
OMAiDMANMEG.
OrthoDBiEOG7KDFK9.

Enzyme and pathway databases

BioCyciYEAST:G3O-31343-MONOMER.

Miscellaneous databases

PROiP04801.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the gene for the yeast cytoplasmic threonyl-tRNA synthetase."
    Pape L.K., Tzagoloff A.
    Nucleic Acids Res. 13:6171-6183(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-289; THR-381; SER-453; SER-457; THR-460 AND SER-605, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195; SER-289; THR-297; SER-453 AND SER-457, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYTC_YEAST
AccessioniPrimary (citable) accession number: P04801
Secondary accession number(s): D6VVK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 42600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.