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Protein

Threonine--tRNA ligase, cytoplasmic

Gene

THS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 42600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • threonine-tRNA ligase activity Source: SGD

GO - Biological processi

  • threonyl-tRNA aminoacylation Source: SGD

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31343-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase, cytoplasmic (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Gene namesi
Name:THS1
Ordered Locus Names:YIL078W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL078W.
SGDiS000001340. THS1.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001011251 – 734Threonine--tRNA ligase, cytoplasmicAdd BLAST734

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei195PhosphoserineCombined sources1
Modified residuei289PhosphoserineCombined sources1
Modified residuei297PhosphothreonineCombined sources1
Modified residuei381PhosphothreonineCombined sources1
Modified residuei453PhosphoserineCombined sources1
Modified residuei457PhosphoserineCombined sources1
Modified residuei460PhosphothreonineCombined sources1
Modified residuei605PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04801.
PRIDEiP04801.

PTM databases

iPTMnetiP04801.

Interactioni

Protein-protein interaction databases

BioGridi34914. 279 interactors.
DIPiDIP-5307N.
IntActiP04801. 17 interactors.
MINTiMINT-540497.
STRINGi4932.YIL078W.

Structurei

3D structure databases

ProteinModelPortaliP04801.
SMRiP04801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000002149.
HOGENOMiHOG000003878.
InParanoidiP04801.
KOiK01868.
OMAiFYYDFAY.
OrthoDBiEOG092C32CL.

Family and domain databases

CDDicd00771. ThrRS_core. 1 hit.
Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR036621. Anticodon-bd_dom_sf.
IPR012675. Beta-grasp_dom_sf.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR033728. ThrRS_core.
IPR012947. tRNA_SAD.
PANTHERiPTHR11451. PTHR11451. 1 hit.
PfamiView protein in Pfam
PF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiView protein in SMART
SM00863. tRNA_SAD. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiView protein in PROSITE
PS50862. AA_TRNA_LIGASE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

P04801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASEAGVTE QVKKLSVKDS SNDAVKPNKK ENKKSKQQSL YLDPEPTFIE
60 70 80 90 100
ERIEMFDRLQ KEYNDKVASM PRVPLKIVLK DGAVKEATSW ETTPMDIAKG
110 120 130 140 150
ISKSLADRLC ISKVNGQLWD LDRPFEGEAN EEIKLELLDF ESDEGKKVFW
160 170 180 190 200
HSSAHVLGES CECHLGAHIC LGPPTDDGFF YEMAVRDSMK DISESPERTV
210 220 230 240 250
SQADFPGLEG VAKNVIKQKQ KFERLVMSKE DLLKMFHYSK YKTYLVQTKV
260 270 280 290 300
PDGGATTVYR CGKLIDLCVG PHIPHTGRIK AFKLLKNSSC YFLGDATNDS
310 320 330 340 350
LQRVYGISFP DKKLMDAHLK FLAEASMRDH RKIGKEQELF LFNEMSPGSC
360 370 380 390 400
FWLPHGTRIY NTLVDLLRTE YRKRGYEEVI TPNMYNSKLW ETSGHWANYK
410 420 430 440 450
ENMFTFEVEK ETFGLKPMNC PGHCLMFKSR ERSYRELPWR VADFGVIHRN
460 470 480 490 500
EFSGALSGLT RVRRFQQDDA HIFCTHDQIE SEIENIFNFL QYIYGVFGFE
510 520 530 540 550
FKMELSTRPE KYVGKIETWD AAESKLESAL KKWGGNWEIN AGDGAFYGPK
560 570 580 590 600
IDIMISDALR RWHQCATIQL DFQLPNRFEL EFKSKDQDSE SYERPVMIHR
610 620 630 640 650
AILGSVERMT AILTEHFAGK WPFWLSPRQV LVVPVGVKYQ GYAEDVRNKL
660 670 680 690 700
HDAGFYADVD LTGNTLQKKV RNGQMLKYNF IFIVGEQEMN EKSVNIRNRD
710 720 730
VMEQQGKNAT VSVEEVLKQL RNLKDEKRGD NVLA
Length:734
Mass (Da):84,520
Last modified:February 1, 1995 - v2
Checksum:iEA328F8EC08417C3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9T → A in CAA26666 (PubMed:2995918).Curated1
Sequence conflicti18K → N in CAA26666 (PubMed:2995918).Curated1
Sequence conflicti502K → E in CAA26666 (PubMed:2995918).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37997 Genomic DNA. Translation: CAA86092.1.
X02906 Genomic DNA. Translation: CAA26666.1.
BK006942 Genomic DNA. Translation: DAA08472.1.
PIRiS48366. YSBYTC.
RefSeqiNP_116578.3. NM_001179428.3.

Genome annotation databases

EnsemblFungiiYIL078W; YIL078W; YIL078W.
GeneIDi854732.
KEGGisce:YIL078W.

Similar proteinsi

Entry informationi

Entry nameiSYTC_YEAST
AccessioniPrimary (citable) accession number: P04801
Secondary accession number(s): D6VVK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 1, 1995
Last modified: November 22, 2017
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names