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P04800

- CP3A1_RAT

UniProt

P04800 - CP3A1_RAT

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Protein

Cytochrome P450 3A1

Gene

Cyp3a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi443 – 4431Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. demethylase activity Source: RGD
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. testosterone 6-beta-hydroxylase activity Source: RGD

GO - Biological processi

  1. aging Source: RGD
  2. drug metabolic process Source: RGD
  3. oxidative demethylation Source: RGD
  4. response to cadmium ion Source: RGD
  5. response to drug Source: RGD
  6. response to glucocorticoid Source: RGD
  7. response to metal ion Source: RGD
  8. response to nutrient Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 3A1 (EC:1.14.14.1)
Alternative name(s):
CYPIIIA1
Cytochrome P450-PCN1
Gene namesi
Name:Cyp3a1
Synonyms:Cyp3a-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi628626. Cyp3a1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: RGD
  2. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Cytochrome P450 3A1PRO_0000051783Add
BLAST

Proteomic databases

PaxDbiP04800.
PRIDEiP04800.

PTM databases

PhosphoSiteiP04800.

Expressioni

Inductioni

By pregnenolone 16-alpha-carbonitrile (PNCN).

Gene expression databases

GenevestigatoriP04800.

Interactioni

Protein-protein interaction databases

MINTiMINT-4570555.

Structurei

3D structure databases

ProteinModelPortaliP04800.
SMRiP04800. Positions 30-497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000039127.
HOVERGENiHBG108567.
InParanoidiP04800.
PhylomeDBiP04800.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04800-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLLSALTLE TWVLLAVVLV LLYGFGTRTH GLFKKQGIPG PKPLPFFGTV
60 70 80 90 100
LNYYMGLWKF DVECHKKYGK IWGLFDGQMP LFAITDTEMI KNVLVKECFS
110 120 130 140 150
VFTNRRDFGP VGIMGKAVSV AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE
160 170 180 190 200
QYGDILVKYL KQEAETGKPV TMKKVFGAYS MDVITSTSFG VNVDSLNNPK
210 220 230 240 250
DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM FPKDSIEFFK
260 270 280 290 300
KFVYRMKETR LDSVQKHRVD FLQLMMNAHN DSKDKESHTA LSDMEITAQS
310 320 330 340 350
IIFIFAGYEP TSSTLSFVLH SLATHPDTQK KLQEEIDRAL PNKAPPTYDT
360 370 380 390 400
VMEMEYLDMV LNETLRLYPI GNRLERVCKK DVEINGVFMP KGSVVMIPSY
410 420 430 440 450
ALHRDPQHWP EPEEFRPERF SKENKGSIDP YVYLPFGNGP RNCIGMRFAL
460 470 480 490 500
MNMKLALTKV LQNFSFQPCK ETQIPLKLSR QGLLQPTKPI ILKVVPRDEI

ITGS
Length:504
Mass (Da):57,918
Last modified:August 13, 1987 - v1
Checksum:iCFD5AC8C37E9CADB
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti207 – 2071T → A in strain: Wistar.
Natural varianti213 – 2131F → I in strain: Wistar.
Natural varianti232 – 2321I → V in strain: Wistar.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10161 mRNA. Translation: AAA41035.1.
X64401 mRNA. Translation: CAA45743.1.
M86850 Genomic DNA. Translation: AAA41780.1.
X62086 Genomic DNA. No translation available.
PIRiA22631.
UniGeneiRn.91120.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10161 mRNA. Translation: AAA41035.1 .
X64401 mRNA. Translation: CAA45743.1 .
M86850 Genomic DNA. Translation: AAA41780.1 .
X62086 Genomic DNA. No translation available.
PIRi A22631.
UniGenei Rn.91120.

3D structure databases

ProteinModelPortali P04800.
SMRi P04800. Positions 30-497.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4570555.

Chemistry

BindingDBi P04800.
ChEMBLi CHEMBL3323.
DrugBanki DB08834. Tauroursodeoxycholic acid.

PTM databases

PhosphoSitei P04800.

Proteomic databases

PaxDbi P04800.
PRIDEi P04800.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

RGDi 628626. Cyp3a1.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000039127.
HOVERGENi HBG108567.
InParanoidi P04800.
PhylomeDBi P04800.

Gene expression databases

Genevestigatori P04800.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete cDNA and protein sequence of a pregnenolone 16 alpha-carbonitrile-induced cytochrome P-450. A representative of a new gene family."
    Gonzalez F.J., Nebert D.W., Hardwick J.P., Kasper C.B.
    J. Biol. Chem. 260:7435-7441(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and characterization of a novel CYP3A1 allelic variant: analysis of CYP3A1 and CYP3A2 sex-hormone-dependent expression reveals that the CYP3A2 gene is regulated by testosterone."
    Ribeiro V., Lechner M.C.
    Arch. Biochem. Biophys. 293:147-152(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  3. "Purification and characterization of four catalytically active testosterone 6 beta-hydroxylase P-450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms."
    Nagata K., Gonzalez F.J., Yamazoe Y., Kato R.
    J. Biochem. 107:718-725(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-26.
  4. "Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats."
    Cooper K.O., Reik L.M., Jayyosi Z., Bandiera S., Kelley M., Ryan D.E., Daniel R., McCluskey S.A., Levin W., Thomas P.E.
    Arch. Biochem. Biophys. 301:345-354(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-25.
  5. "Paradoxical transcriptional activation of rat liver cytochrome P-450 3A1 by dexamethasone and the antiglucocorticoid pregnenolone 16 alpha-carbonitrile: analysis by transient transfection into primary monolayer cultures of adult rat hepatocytes."
    Burger H.J., Schuetz J.D., Schuetz E.G., Guzelian P.S.
    Proc. Natl. Acad. Sci. U.S.A. 89:2145-2149(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
  6. "Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development."
    Telhada M.B., Pereira T.M., Lechner M.C.
    Arch. Biochem. Biophys. 298:715-725(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-24.
    Tissue: Liver.

Entry informationi

Entry nameiCP3A1_RAT
AccessioniPrimary (citable) accession number: P04800
Secondary accession number(s): Q64580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 26, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3