P04800 (CP3A1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 3A1
EC=1.14.14.1

Alternative name(s):
CYPIIIA1
Cytochrome P450-PCN1

Gene names

Name:	Cyp3a1
Synonyms:	Cyp3a-1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By pregnenolone 16-alpha-carbonitrile (PNCN).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from expression pattern PubMed 16207635. Source: RGD drug metabolic process Inferred from direct assay PubMed 12971802. Source: RGD oxidative demethylation Inferred from direct assay PubMed 12971802. Source: RGD response to cadmium ion Inferred from expression pattern PubMed 14595535. Source: RGD response to drug Inferred from expression pattern PubMed 17484520. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern PubMed 15349958. Source: RGD response to nutrient Inferred from expression pattern PubMed 16380645. Source: RGD
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay PubMed 8841512. Source: RGD
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC demethylase activity Inferred from direct assay PubMed 12971802PubMed 18057719. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro testosterone 6-beta-hydroxylase activity Inferred from direct assay PubMed 9989933. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Cytochrome P450 3A1		PRO_0000051783
Sites
	Metal binding	443	1	Iron (heme axial ligand)
Natural variations
	Natural variant	207	1	T → A in strain: Wistar.
	Natural variant	213	1	F → I in strain: Wistar.
	Natural variant	232	1	I → V in strain: Wistar.

Sequences

	Sequence		Length	Mass (Da)
	P04800 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: CFD5AC8C37E9CADB	FASTA	504	57,918
	10 20 30 40 50 60 MDLLSALTLE TWVLLAVVLV LLYGFGTRTH GLFKKQGIPG PKPLPFFGTV LNYYMGLWKF 70 80 90 100 110 120 DVECHKKYGK IWGLFDGQMP LFAITDTEMI KNVLVKECFS VFTNRRDFGP VGIMGKAVSV 130 140 150 160 170 180 AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE QYGDILVKYL KQEAETGKPV TMKKVFGAYS 190 200 210 220 230 240 MDVITSTSFG VNVDSLNNPK DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM 250 260 270 280 290 300 FPKDSIEFFK KFVYRMKETR LDSVQKHRVD FLQLMMNAHN DSKDKESHTA LSDMEITAQS 310 320 330 340 350 360 IIFIFAGYEP TSSTLSFVLH SLATHPDTQK KLQEEIDRAL PNKAPPTYDT VMEMEYLDMV 370 380 390 400 410 420 LNETLRLYPI GNRLERVCKK DVEINGVFMP KGSVVMIPSY ALHRDPQHWP EPEEFRPERF 430 440 450 460 470 480 SKENKGSIDP YVYLPFGNGP RNCIGMRFAL MNMKLALTKV LQNFSFQPCK ETQIPLKLSR 490 500 QGLLQPTKPI ILKVVPRDEI ITGS

References

[1]	"Complete cDNA and protein sequence of a pregnenolone 16 alpha-carbonitrile-induced cytochrome P-450. A representative of a new gene family." Gonzalez F.J., Nebert D.W., Hardwick J.P., Kasper C.B. J. Biol. Chem. 260:7435-7441(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and characterization of a novel CYP3A1 allelic variant: analysis of CYP3A1 and CYP3A2 sex-hormone-dependent expression reveals that the CYP3A2 gene is regulated by testosterone." Ribeiro V., Lechner M.C. Arch. Biochem. Biophys. 293:147-152(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[3]	"Purification and characterization of four catalytically active testosterone 6 beta-hydroxylase P-450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms." Nagata K., Gonzalez F.J., Yamazoe Y., Kato R. J. Biochem. 107:718-725(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-26.
[4]	"Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats." Cooper K.O., Reik L.M., Jayyosi Z., Bandiera S., Kelley M., Ryan D.E., Daniel R., McCluskey S.A., Levin W., Thomas P.E. Arch. Biochem. Biophys. 301:345-354(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25.
[5]	"Paradoxical transcriptional activation of rat liver cytochrome P-450 3A1 by dexamethasone and the antiglucocorticoid pregnenolone 16 alpha-carbonitrile: analysis by transient transfection into primary monolayer cultures of adult rat hepatocytes." Burger H.J., Schuetz J.D., Schuetz E.G., Guzelian P.S. Proc. Natl. Acad. Sci. U.S.A. 89:2145-2149(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
[6]	"Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development." Telhada M.B., Pereira T.M., Lechner M.C. Arch. Biochem. Biophys. 298:715-725(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-24. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M10161 mRNA. Translation: AAA41035.1. X64401 mRNA. Translation: CAA45743.1. M86850 Genomic DNA. Translation: AAA41780.1. X62086 Genomic DNA. No translation available.
IPI	IPI00199589.
PIR	A22631.
UniGene	Rn.91120.
3D structure databases
ProteinModelPortal	P04800.
SMR	P04800. Positions 30-497.
ModBase	Search...
PTM databases
PhosphoSite	P04800.
Proteomic databases
PaxDb	P04800.
PRIDE	P04800.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
RGD	628626. Cyp3a1.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000039127.
HOVERGEN	HBG108567.
InParanoid	P04800.
OrthoDB	EOG4ZW59X.
Gene expression databases
ArrayExpress	P04800.
Genevestigator	P04800.
GermOnline	ENSRNOG00000032560. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR008072. Cyt_P450_E_CYP3A. IPR002402. Cyt_P450_E_grp-II. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00464. EP450II. PR01689. EP450IICYP3A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P04800.
ChEMBL	CHEMBL3323.

Entry information

Entry name

CP3A1_RAT

Accession

Primary (citable) accession number: P04800
Secondary accession number(s): Q64580

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program