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P04800

- CP3A1_RAT

UniProt

P04800 - CP3A1_RAT

Protein

Cytochrome P450 3A1

Gene

Cyp3a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi443 – 4431Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. demethylase activity Source: RGD
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. testosterone 6-beta-hydroxylase activity Source: RGD

    GO - Biological processi

    1. aging Source: RGD
    2. drug metabolic process Source: RGD
    3. oxidative demethylation Source: RGD
    4. response to cadmium ion Source: RGD
    5. response to drug Source: RGD
    6. response to glucocorticoid Source: RGD
    7. response to metal ion Source: RGD
    8. response to nutrient Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 3A1 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIIA1
    Cytochrome P450-PCN1
    Gene namesi
    Name:Cyp3a1
    Synonyms:Cyp3a-1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi628626. Cyp3a1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: RGD
    2. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504Cytochrome P450 3A1PRO_0000051783Add
    BLAST

    Proteomic databases

    PaxDbiP04800.
    PRIDEiP04800.

    PTM databases

    PhosphoSiteiP04800.

    Expressioni

    Inductioni

    By pregnenolone 16-alpha-carbonitrile (PNCN).

    Gene expression databases

    GenevestigatoriP04800.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4570555.

    Structurei

    3D structure databases

    ProteinModelPortaliP04800.
    SMRiP04800. Positions 30-497.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000039127.
    HOVERGENiHBG108567.
    InParanoidiP04800.
    PhylomeDBiP04800.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR008072. Cyt_P450_E_CYP3A.
    IPR002402. Cyt_P450_E_grp-II.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00464. EP450II.
    PR01689. EP450IICYP3A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04800-1 [UniParc]FASTAAdd to Basket

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    MDLLSALTLE TWVLLAVVLV LLYGFGTRTH GLFKKQGIPG PKPLPFFGTV    50
    LNYYMGLWKF DVECHKKYGK IWGLFDGQMP LFAITDTEMI KNVLVKECFS 100
    VFTNRRDFGP VGIMGKAVSV AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE 150
    QYGDILVKYL KQEAETGKPV TMKKVFGAYS MDVITSTSFG VNVDSLNNPK 200
    DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM FPKDSIEFFK 250
    KFVYRMKETR LDSVQKHRVD FLQLMMNAHN DSKDKESHTA LSDMEITAQS 300
    IIFIFAGYEP TSSTLSFVLH SLATHPDTQK KLQEEIDRAL PNKAPPTYDT 350
    VMEMEYLDMV LNETLRLYPI GNRLERVCKK DVEINGVFMP KGSVVMIPSY 400
    ALHRDPQHWP EPEEFRPERF SKENKGSIDP YVYLPFGNGP RNCIGMRFAL 450
    MNMKLALTKV LQNFSFQPCK ETQIPLKLSR QGLLQPTKPI ILKVVPRDEI 500
    ITGS 504
    Length:504
    Mass (Da):57,918
    Last modified:August 13, 1987 - v1
    Checksum:iCFD5AC8C37E9CADB
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti207 – 2071T → A in strain: Wistar.
    Natural varianti213 – 2131F → I in strain: Wistar.
    Natural varianti232 – 2321I → V in strain: Wistar.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10161 mRNA. Translation: AAA41035.1.
    X64401 mRNA. Translation: CAA45743.1.
    M86850 Genomic DNA. Translation: AAA41780.1.
    X62086 Genomic DNA. No translation available.
    PIRiA22631.
    UniGeneiRn.91120.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10161 mRNA. Translation: AAA41035.1 .
    X64401 mRNA. Translation: CAA45743.1 .
    M86850 Genomic DNA. Translation: AAA41780.1 .
    X62086 Genomic DNA. No translation available.
    PIRi A22631.
    UniGenei Rn.91120.

    3D structure databases

    ProteinModelPortali P04800.
    SMRi P04800. Positions 30-497.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4570555.

    Chemistry

    BindingDBi P04800.
    ChEMBLi CHEMBL3323.

    PTM databases

    PhosphoSitei P04800.

    Proteomic databases

    PaxDbi P04800.
    PRIDEi P04800.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    RGDi 628626. Cyp3a1.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000039127.
    HOVERGENi HBG108567.
    InParanoidi P04800.
    PhylomeDBi P04800.

    Gene expression databases

    Genevestigatori P04800.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR008072. Cyt_P450_E_CYP3A.
    IPR002402. Cyt_P450_E_grp-II.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00464. EP450II.
    PR01689. EP450IICYP3A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete cDNA and protein sequence of a pregnenolone 16 alpha-carbonitrile-induced cytochrome P-450. A representative of a new gene family."
      Gonzalez F.J., Nebert D.W., Hardwick J.P., Kasper C.B.
      J. Biol. Chem. 260:7435-7441(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and characterization of a novel CYP3A1 allelic variant: analysis of CYP3A1 and CYP3A2 sex-hormone-dependent expression reveals that the CYP3A2 gene is regulated by testosterone."
      Ribeiro V., Lechner M.C.
      Arch. Biochem. Biophys. 293:147-152(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Liver.
    3. "Purification and characterization of four catalytically active testosterone 6 beta-hydroxylase P-450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms."
      Nagata K., Gonzalez F.J., Yamazoe Y., Kato R.
      J. Biochem. 107:718-725(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-26.
    4. "Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats."
      Cooper K.O., Reik L.M., Jayyosi Z., Bandiera S., Kelley M., Ryan D.E., Daniel R., McCluskey S.A., Levin W., Thomas P.E.
      Arch. Biochem. Biophys. 301:345-354(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-25.
    5. "Paradoxical transcriptional activation of rat liver cytochrome P-450 3A1 by dexamethasone and the antiglucocorticoid pregnenolone 16 alpha-carbonitrile: analysis by transient transfection into primary monolayer cultures of adult rat hepatocytes."
      Burger H.J., Schuetz J.D., Schuetz E.G., Guzelian P.S.
      Proc. Natl. Acad. Sci. U.S.A. 89:2145-2149(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
    6. "Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development."
      Telhada M.B., Pereira T.M., Lechner M.C.
      Arch. Biochem. Biophys. 298:715-725(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 1-24.
      Tissue: Liver.

    Entry informationi

    Entry nameiCP3A1_RAT
    AccessioniPrimary (citable) accession number: P04800
    Secondary accession number(s): Q64580
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3