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P04800 (CP3A1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 3A1

EC=1.14.14.1
Alternative name(s):
CYPIIIA1
Cytochrome P450-PCN1
Gene names
Name:Cyp3a1
Synonyms:Cyp3a-1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

By pregnenolone 16-alpha-carbonitrile (PNCN).

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityPolymorphism
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from expression pattern PubMed 16207635. Source: RGD

drug metabolic process

Inferred from direct assay PubMed 12971802. Source: RGD

oxidative demethylation

Inferred from direct assay PubMed 12971802. Source: RGD

response to cadmium ion

Inferred from expression pattern PubMed 14595535. Source: RGD

response to drug

Inferred from expression pattern PubMed 17484520. Source: RGD

response to glucocorticoid

Inferred from expression pattern PubMed 15349958. Source: RGD

response to metal ion

Inferred from expression pattern PubMed 16039771. Source: RGD

response to nutrient

Inferred from expression pattern PubMed 16380645. Source: RGD

   Cellular_componentendoplasmic reticulum membrane

Inferred from direct assay PubMed 8841512. Source: RGD

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 8841512. Source: RGD

   Molecular_functionaromatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

demethylase activity

Inferred from direct assay PubMed 12971802PubMed 18057719. Source: RGD

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

testosterone 6-beta-hydroxylase activity

Inferred from direct assay PubMed 9989933. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504Cytochrome P450 3A1
PRO_0000051783

Sites

Metal binding4431Iron (heme axial ligand)

Natural variations

Natural variant2071T → A in strain: Wistar.
Natural variant2131F → I in strain: Wistar.
Natural variant2321I → V in strain: Wistar.

Sequences

Sequence LengthMass (Da)Tools
P04800 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: CFD5AC8C37E9CADB

FASTA50457,918
        10         20         30         40         50         60 
MDLLSALTLE TWVLLAVVLV LLYGFGTRTH GLFKKQGIPG PKPLPFFGTV LNYYMGLWKF 

        70         80         90        100        110        120 
DVECHKKYGK IWGLFDGQMP LFAITDTEMI KNVLVKECFS VFTNRRDFGP VGIMGKAVSV 

       130        140        150        160        170        180 
AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE QYGDILVKYL KQEAETGKPV TMKKVFGAYS 

       190        200        210        220        230        240 
MDVITSTSFG VNVDSLNNPK DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM 

       250        260        270        280        290        300 
FPKDSIEFFK KFVYRMKETR LDSVQKHRVD FLQLMMNAHN DSKDKESHTA LSDMEITAQS 

       310        320        330        340        350        360 
IIFIFAGYEP TSSTLSFVLH SLATHPDTQK KLQEEIDRAL PNKAPPTYDT VMEMEYLDMV 

       370        380        390        400        410        420 
LNETLRLYPI GNRLERVCKK DVEINGVFMP KGSVVMIPSY ALHRDPQHWP EPEEFRPERF 

       430        440        450        460        470        480 
SKENKGSIDP YVYLPFGNGP RNCIGMRFAL MNMKLALTKV LQNFSFQPCK ETQIPLKLSR 

       490        500 
QGLLQPTKPI ILKVVPRDEI ITGS 

« Hide

References

[1]"Complete cDNA and protein sequence of a pregnenolone 16 alpha-carbonitrile-induced cytochrome P-450. A representative of a new gene family."
Gonzalez F.J., Nebert D.W., Hardwick J.P., Kasper C.B.
J. Biol. Chem. 260:7435-7441(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and characterization of a novel CYP3A1 allelic variant: analysis of CYP3A1 and CYP3A2 sex-hormone-dependent expression reveals that the CYP3A2 gene is regulated by testosterone."
Ribeiro V., Lechner M.C.
Arch. Biochem. Biophys. 293:147-152(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Liver.
[3]"Purification and characterization of four catalytically active testosterone 6 beta-hydroxylase P-450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms."
Nagata K., Gonzalez F.J., Yamazoe Y., Kato R.
J. Biochem. 107:718-725(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-26.
[4]"Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats."
Cooper K.O., Reik L.M., Jayyosi Z., Bandiera S., Kelley M., Ryan D.E., Daniel R., McCluskey S.A., Levin W., Thomas P.E.
Arch. Biochem. Biophys. 301:345-354(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-25.
[5]"Paradoxical transcriptional activation of rat liver cytochrome P-450 3A1 by dexamethasone and the antiglucocorticoid pregnenolone 16 alpha-carbonitrile: analysis by transient transfection into primary monolayer cultures of adult rat hepatocytes."
Burger H.J., Schuetz J.D., Schuetz E.G., Guzelian P.S.
Proc. Natl. Acad. Sci. U.S.A. 89:2145-2149(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
[6]"Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development."
Telhada M.B., Pereira T.M., Lechner M.C.
Arch. Biochem. Biophys. 298:715-725(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-24.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M10161 mRNA. Translation: AAA41035.1.
X64401 mRNA. Translation: CAA45743.1.
M86850 Genomic DNA. Translation: AAA41780.1.
X62086 Genomic DNA. No translation available.
PIRA22631.
UniGeneRn.91120.

3D structure databases

ProteinModelPortalP04800.
SMRP04800. Positions 30-497.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4570555.

Chemistry

BindingDBP04800.
ChEMBLCHEMBL3323.

PTM databases

PhosphoSiteP04800.

Proteomic databases

PaxDbP04800.
PRIDEP04800.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD628626. Cyp3a1.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000039127.
HOVERGENHBG108567.
InParanoidP04800.
PhylomeDBP04800.

Gene expression databases

GenevestigatorP04800.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP3A1_RAT
AccessionPrimary (citable) accession number: P04800
Secondary accession number(s): Q64580
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 16, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families