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P04799

- CP1A2_RAT

UniProt

P04799 - CP1A2_RAT

Protein

Cytochrome P450 1A2

Gene

Cyp1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.By similarity

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi456 – 4561Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. caffeine oxidase activity Source: Ensembl
    3. demethylase activity Source: Ensembl
    4. heme binding Source: UniProtKB
    5. iron ion binding Source: InterPro
    6. monooxygenase activity Source: RGD
    7. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: RGD

    GO - Biological processi

    1. alkaloid metabolic process Source: Ensembl
    2. cellular respiration Source: Ensembl
    3. cellular response to cadmium ion Source: Ensembl
    4. dibenzo-p-dioxin metabolic process Source: Ensembl
    5. exogenous drug catabolic process Source: Ensembl
    6. hydrogen peroxide biosynthetic process Source: Ensembl
    7. lung development Source: Ensembl
    8. monocarboxylic acid metabolic process Source: Ensembl
    9. monoterpenoid metabolic process Source: Ensembl
    10. oxidative deethylation Source: Ensembl
    11. oxidative demethylation Source: Ensembl
    12. porphyrin-containing compound metabolic process Source: Ensembl
    13. post-embryonic development Source: Ensembl
    14. regulation of gene expression Source: Ensembl
    15. response to drug Source: RGD
    16. response to estradiol Source: RGD
    17. response to immobilization stress Source: RGD
    18. response to lipopolysaccharide Source: RGD
    19. response to organic cyclic compound Source: RGD
    20. response to organic substance Source: RGD
    21. steroid catabolic process Source: Ensembl
    22. toxin biosynthetic process Source: Ensembl
    23. xenobiotic metabolic process Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198836. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
    REACT_198844. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
    SABIO-RKP04799.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 1A2 (EC:1.14.14.1)
    Alternative name(s):
    CYPIA2
    Cytochrome P-448
    Cytochrome P-450d
    Cytochrome P450-D
    Gene namesi
    Name:Cyp1a2
    Synonyms:Cyp1a-2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi2459. Cyp1a2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. intracellular membrane-bounded organelle Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 513512Cytochrome P450 1A2PRO_0000051656Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi68 – 681O-linked (GlcNAc)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP04799.

    Expressioni

    Inductioni

    By 3-methylcholanthrene (3MC).

    Gene expression databases

    GenevestigatoriP04799.

    Interactioni

    Protein-protein interaction databases

    BioGridi246478. 1 interaction.
    MINTiMINT-4564487.

    Structurei

    3D structure databases

    ProteinModelPortaliP04799.
    SMRiP04799. Positions 33-512.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00750000117317.
    HOVERGENiHBG106944.
    KOiK07409.
    OMAiAFILEIY.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiP04799.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008066. Cyt_P450_E_grp-I_CYP1.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01683. EP450ICYP1A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04799-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFSQYISLA PELLLATAIF CLVFWVLRGT RTQVPKGLKS PPGPWGLPFI    50
    GHMLTLGKNP HLSLTKLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVKQ 100
    GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS 150
    DPTSVSSCYL EEHVSKEANH LISKFQKLMA EVGHFEPVNQ VVESVANVIG 200
    AMCFGKNFPR KSEEMLNLVK SSKDFVENVT SGNAVDFFPV LRYLPNPALK 250
    RFKNFNDNFV LFLQKTVQEH YQDFNKNSIQ DITGALFKHS ENYKDNGGLI 300
    PQEKIVNIVN DIFGAGFETV TTAIFWSILL LVTEPKVQRK IHEELDTVIG 350
    RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP 400
    KECCIFINQW QVNHDEKQWK DPFVFRPERF LTNDNTAIDK TLSEKVMLFG 450
    LGKRRCIGEI PAKWEVFLFL AILLHQLEFT VPPGVKVDLT PSYGLTMKPR 500
    TCEHVQAWPR FSK 513
    Length:513
    Mass (Da):58,259
    Last modified:May 1, 2007 - v2
    Checksum:iB1F06FDE9B7B6FA7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261V → M AA sequence (PubMed:3718958)Curated
    Sequence conflicti137 – 1371R → H in AAA41053. (PubMed:2985574)Curated
    Sequence conflicti137 – 1371R → H no nucleotide entry (PubMed:1793487)Curated
    Sequence conflicti185 – 1851F → L no nucleotide entry (PubMed:1793487)Curated
    Sequence conflicti262 – 2621F → S in AAA41028. (PubMed:6584898)Curated
    Sequence conflicti403 – 4031C → R in AAA41053. (PubMed:2985574)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02422 mRNA. Translation: AAA41028.1.
    K03241 Genomic DNA. Translation: AAA41053.1.
    BC127476 mRNA. Translation: AAI27477.1.
    X01031 mRNA. Translation: CAA25516.1.
    PIRiA61400.
    RefSeqiNP_036673.3. NM_012541.3.
    UniGeneiRn.5563.

    Genome annotation databases

    EnsembliENSRNOT00000021653; ENSRNOP00000021653; ENSRNOG00000016173.
    GeneIDi24297.
    KEGGirno:24297.
    UCSCiRGD:2459. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02422 mRNA. Translation: AAA41028.1 .
    K03241 Genomic DNA. Translation: AAA41053.1 .
    BC127476 mRNA. Translation: AAI27477.1 .
    X01031 mRNA. Translation: CAA25516.1 .
    PIRi A61400.
    RefSeqi NP_036673.3. NM_012541.3.
    UniGenei Rn.5563.

    3D structure databases

    ProteinModelPortali P04799.
    SMRi P04799. Positions 33-512.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246478. 1 interaction.
    MINTi MINT-4564487.

    Chemistry

    ChEMBLi CHEMBL1075125.

    Proteomic databases

    PRIDEi P04799.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000021653 ; ENSRNOP00000021653 ; ENSRNOG00000016173 .
    GeneIDi 24297.
    KEGGi rno:24297.
    UCSCi RGD:2459. rat.

    Organism-specific databases

    CTDi 1544.
    RGDi 2459. Cyp1a2.

    Phylogenomic databases

    GeneTreei ENSGT00750000117317.
    HOVERGENi HBG106944.
    KOi K07409.
    OMAi AFILEIY.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi P04799.

    Enzyme and pathway databases

    Reactomei REACT_198836. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
    REACT_198844. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
    SABIO-RK P04799.

    Miscellaneous databases

    NextBioi 602906.
    PROi P04799.

    Gene expression databases

    Genevestigatori P04799.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008066. Cyt_P450_E_grp-I_CYP1.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01683. EP450ICYP1A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Coding nucleotide sequence of 3-methylcholanthrene-inducible cytochrome P-450d cDNA from rat liver."
      Kawajiri K., Gotoh O., Sogawa K., Tagashira Y., Muramatsu M., Fujii-Kuriyama Y.
      Proc. Natl. Acad. Sci. U.S.A. 81:1649-1653(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Complete nucleotide sequence of a methylcholanthrene-inducible cytochrome P-450 (P-450d) gene in the rat."
      Sogawa K., Gotoh O., Kawajiri K., Harada T., Fujii-Kuriyama Y.
      J. Biol. Chem. 260:5026-5032(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Stable expression of rat cytochrome P450IA2 cDNA and hydroxylation of 17 beta-estradiol and 2-aminofluorene in V79 Chinese hamster cells."
      Wolfel C., Platt K.L., Dogra S., Glatt H., Wachter F., Doehmer J.
      Mol. Carcinog. 4:489-498(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    5. "The primary structure of cytochrome P-450d purified from rat liver microsomes: prediction of helical regions and domain analysis."
      Haniu M., Ryan D.E., Levin W., Shively J.E.
      Arch. Biochem. Biophys. 244:323-337(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Tissue: Liver.
    6. "Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450."
      Cheng K.C., Park S.S., Krutzsch H.C., Grantham P.H., Gelboin H.V., Friedman F.K.
      Biochemistry 25:2397-2402(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-26.
    7. "Amino-terminal and carboxy-terminal sequence of hepatic microsomal cytochrome P-450d, a unique hemoprotein from rats treated with isosafrole."
      Botelho L.H., Ryan D.E., Yuan P.M., Kutny R., Shively J.E., Levin W.
      Biochemistry 21:1152-1155(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-20.
    8. "Modification of cytochrome P450 1A2 enzymes by the mechanism-based inactivator 2-ethynylnaphthalene and the photoaffinity label 4-azidobiphenyl."
      Yun C.H., Hammons G.J., Jones G., Martin M.V., Hopkins N.E., Alworth W.L., Guengerich F.P.
      Biochemistry 31:10556-10563(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 67-78.
    9. "Characterization of complementary DNA clones coding for two forms of 3-methylcholanthrene-inducible rat liver cytochrome P-450."
      Yabusaki Y., Murakami H., Nakamura K., Nomura N., Shimizu M., Oeda K., Ohkawa H.
      J. Biochem. 96:793-804(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 147-513.
    10. "Discovery and confirmation of O-GlcNAcylated proteins in rat liver mitochondria by combination of mass spectrometry and immunological methods."
      Cao W., Cao J., Huang J., Yao J., Yan G., Xu H., Yang P.
      PLoS ONE 8:E76399-E76399(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT SER-68.

    Entry informationi

    Entry nameiCP1A2_RAT
    AccessioniPrimary (citable) accession number: P04799
    Secondary accession number(s): A1L120, Q64588
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3