Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytochrome P450 1A2

Gene

Cyp1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation.By similarity

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi456Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processLipid metabolism, Steroid metabolism, Sterol metabolism
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.14.1 5301
ReactomeiR-RNO-156581 Methylation
R-RNO-211957 Aromatic amines can be N-hydroxylated or N-dealkylated by CYP1A2
R-RNO-2142670 Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET)
R-RNO-2142816 Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)
R-RNO-5423646 Aflatoxin activation and detoxification
R-RNO-9018681 Biosynthesis of protectins
R-RNO-9027307 Biosynthesis of maresin-like SPMs
SABIO-RKP04799

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1By similarity)
Alternative name(s):
CYPIA2
Cholesterol 25-hydroxylaseBy similarity
Cytochrome P-448
Cytochrome P-450d
Cytochrome P450-D
Gene namesi
Name:Cyp1a2
Synonyms:Cyp1a-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2459 Cyp1a2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075125

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000516562 – 513Cytochrome P450 1A2Add BLAST512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi68O-linked (GlcNAc) serine1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP04799
PRIDEiP04799

PTM databases

iPTMnetiP04799
PhosphoSitePlusiP04799

Expressioni

Inductioni

By 3-methylcholanthrene (3MC).

Gene expression databases

BgeeiENSRNOG00000016173
GenevisibleiP04799 RN

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi246478, 1 interactor
STRINGi10116.ENSRNOP00000021653

Chemistry databases

BindingDBiP04799

Structurei

3D structure databases

ProteinModelPortaliP04799
SMRiP04799
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
GeneTreeiENSGT00900000140831
HOVERGENiHBG106944
InParanoidiP04799
KOiK07409
OMAiEHHRQGM
OrthoDBiEOG091G0BT8
PhylomeDBiP04799

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR008066 Cyt_P450_E_grp-I_CYP1
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR01683 EP450ICYP1A
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSQYISLA PELLLATAIF CLVFWVLRGT RTQVPKGLKS PPGPWGLPFI
60 70 80 90 100
GHMLTLGKNP HLSLTKLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVKQ
110 120 130 140 150
GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS
160 170 180 190 200
DPTSVSSCYL EEHVSKEANH LISKFQKLMA EVGHFEPVNQ VVESVANVIG
210 220 230 240 250
AMCFGKNFPR KSEEMLNLVK SSKDFVENVT SGNAVDFFPV LRYLPNPALK
260 270 280 290 300
RFKNFNDNFV LFLQKTVQEH YQDFNKNSIQ DITGALFKHS ENYKDNGGLI
310 320 330 340 350
PQEKIVNIVN DIFGAGFETV TTAIFWSILL LVTEPKVQRK IHEELDTVIG
360 370 380 390 400
RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP
410 420 430 440 450
KECCIFINQW QVNHDEKQWK DPFVFRPERF LTNDNTAIDK TLSEKVMLFG
460 470 480 490 500
LGKRRCIGEI PAKWEVFLFL AILLHQLEFT VPPGVKVDLT PSYGLTMKPR
510
TCEHVQAWPR FSK
Length:513
Mass (Da):58,259
Last modified:May 1, 2007 - v2
Checksum:iB1F06FDE9B7B6FA7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26V → M AA sequence (PubMed:3718958).Curated1
Sequence conflicti137R → H in AAA41053 (PubMed:2985574).Curated1
Sequence conflicti137R → H no nucleotide entry (PubMed:1793487).Curated1
Sequence conflicti185F → L no nucleotide entry (PubMed:1793487).Curated1
Sequence conflicti262F → S in AAA41028 (PubMed:6584898).Curated1
Sequence conflicti403C → R in AAA41053 (PubMed:2985574).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02422 mRNA Translation: AAA41028.1
K03241 Genomic DNA Translation: AAA41053.1
BC127476 mRNA Translation: AAI27477.1
X01031 mRNA Translation: CAA25516.1
PIRiA61400
RefSeqiNP_036673.3, NM_012541.3
UniGeneiRn.5563

Genome annotation databases

EnsembliENSRNOT00000021653; ENSRNOP00000021653; ENSRNOG00000016173
GeneIDi24297
KEGGirno:24297
UCSCiRGD:2459 rat

Similar proteinsi

Entry informationi

Entry nameiCP1A2_RAT
AccessioniPrimary (citable) accession number: P04799
Secondary accession number(s): A1L120, Q64588
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health