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P04799

- CP1A2_RAT

UniProt

P04799 - CP1A2_RAT

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Protein

Cytochrome P450 1A2

Gene

Cyp1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation (By similarity).By similarity

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi456 – 4561Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. caffeine oxidase activity Source: Ensembl
  3. demethylase activity Source: Ensembl
  4. heme binding Source: UniProtKB
  5. iron ion binding Source: InterPro
  6. monooxygenase activity Source: RGD
  7. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: RGD

GO - Biological processi

  1. alkaloid metabolic process Source: Ensembl
  2. cellular respiration Source: Ensembl
  3. cellular response to cadmium ion Source: Ensembl
  4. dibenzo-p-dioxin metabolic process Source: Ensembl
  5. exogenous drug catabolic process Source: Ensembl
  6. hydrogen peroxide biosynthetic process Source: Ensembl
  7. lung development Source: Ensembl
  8. monocarboxylic acid metabolic process Source: Ensembl
  9. monoterpenoid metabolic process Source: Ensembl
  10. oxidative deethylation Source: Ensembl
  11. oxidative demethylation Source: Ensembl
  12. porphyrin-containing compound metabolic process Source: Ensembl
  13. post-embryonic development Source: Ensembl
  14. regulation of gene expression Source: Ensembl
  15. response to drug Source: RGD
  16. response to estradiol Source: RGD
  17. response to immobilization stress Source: RGD
  18. response to lipopolysaccharide Source: RGD
  19. response to organic cyclic compound Source: RGD
  20. response to organic substance Source: RGD
  21. steroid catabolic process Source: Ensembl
  22. toxin biosynthetic process Source: Ensembl
  23. xenobiotic metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198836. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
REACT_198844. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
REACT_250512. Aromatic amines can be N-hydroxylated or N-dealkylated by CYP1A2.
REACT_256353. Methylation.
REACT_261168. Aflatoxin activation and detoxification.
SABIO-RKP04799.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1)
Alternative name(s):
CYPIA2
Cytochrome P-448
Cytochrome P-450d
Cytochrome P450-D
Gene namesi
Name:Cyp1a2
Synonyms:Cyp1a-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi2459. Cyp1a2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. intracellular membrane-bounded organelle Source: RGD
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 513512Cytochrome P450 1A2PRO_0000051656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi68 – 681O-linked (GlcNAc)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP04799.

Expressioni

Inductioni

By 3-methylcholanthrene (3MC).

Gene expression databases

ExpressionAtlasiP04799. baseline.
GenevestigatoriP04799.

Interactioni

Protein-protein interaction databases

BioGridi246478. 1 interaction.
MINTiMINT-4564487.

Structurei

3D structure databases

ProteinModelPortaliP04799.
SMRiP04799. Positions 33-512.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118992.
HOVERGENiHBG106944.
InParanoidiP04799.
KOiK07409.
OMAiAFILEIY.
OrthoDBiEOG7RBZ85.
PhylomeDBiP04799.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04799-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFSQYISLA PELLLATAIF CLVFWVLRGT RTQVPKGLKS PPGPWGLPFI
60 70 80 90 100
GHMLTLGKNP HLSLTKLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVKQ
110 120 130 140 150
GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS
160 170 180 190 200
DPTSVSSCYL EEHVSKEANH LISKFQKLMA EVGHFEPVNQ VVESVANVIG
210 220 230 240 250
AMCFGKNFPR KSEEMLNLVK SSKDFVENVT SGNAVDFFPV LRYLPNPALK
260 270 280 290 300
RFKNFNDNFV LFLQKTVQEH YQDFNKNSIQ DITGALFKHS ENYKDNGGLI
310 320 330 340 350
PQEKIVNIVN DIFGAGFETV TTAIFWSILL LVTEPKVQRK IHEELDTVIG
360 370 380 390 400
RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP
410 420 430 440 450
KECCIFINQW QVNHDEKQWK DPFVFRPERF LTNDNTAIDK TLSEKVMLFG
460 470 480 490 500
LGKRRCIGEI PAKWEVFLFL AILLHQLEFT VPPGVKVDLT PSYGLTMKPR
510
TCEHVQAWPR FSK
Length:513
Mass (Da):58,259
Last modified:May 1, 2007 - v2
Checksum:iB1F06FDE9B7B6FA7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261V → M AA sequence (PubMed:3718958)Curated
Sequence conflicti137 – 1371R → H in AAA41053. (PubMed:2985574)Curated
Sequence conflicti137 – 1371R → H no nucleotide entry (PubMed:1793487)Curated
Sequence conflicti185 – 1851F → L no nucleotide entry (PubMed:1793487)Curated
Sequence conflicti262 – 2621F → S in AAA41028. (PubMed:6584898)Curated
Sequence conflicti403 – 4031C → R in AAA41053. (PubMed:2985574)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02422 mRNA. Translation: AAA41028.1.
K03241 Genomic DNA. Translation: AAA41053.1.
BC127476 mRNA. Translation: AAI27477.1.
X01031 mRNA. Translation: CAA25516.1.
PIRiA61400.
RefSeqiNP_036673.3. NM_012541.3.
UniGeneiRn.5563.

Genome annotation databases

EnsembliENSRNOT00000021653; ENSRNOP00000021653; ENSRNOG00000016173.
GeneIDi24297.
KEGGirno:24297.
UCSCiRGD:2459. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02422 mRNA. Translation: AAA41028.1 .
K03241 Genomic DNA. Translation: AAA41053.1 .
BC127476 mRNA. Translation: AAI27477.1 .
X01031 mRNA. Translation: CAA25516.1 .
PIRi A61400.
RefSeqi NP_036673.3. NM_012541.3.
UniGenei Rn.5563.

3D structure databases

ProteinModelPortali P04799.
SMRi P04799. Positions 33-512.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246478. 1 interaction.
MINTi MINT-4564487.

Chemistry

BindingDBi P04799.
ChEMBLi CHEMBL1075125.

Proteomic databases

PRIDEi P04799.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000021653 ; ENSRNOP00000021653 ; ENSRNOG00000016173 .
GeneIDi 24297.
KEGGi rno:24297.
UCSCi RGD:2459. rat.

Organism-specific databases

CTDi 1544.
RGDi 2459. Cyp1a2.

Phylogenomic databases

GeneTreei ENSGT00760000118992.
HOVERGENi HBG106944.
InParanoidi P04799.
KOi K07409.
OMAi AFILEIY.
OrthoDBi EOG7RBZ85.
PhylomeDBi P04799.

Enzyme and pathway databases

Reactomei REACT_198836. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
REACT_198844. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
REACT_250512. Aromatic amines can be N-hydroxylated or N-dealkylated by CYP1A2.
REACT_256353. Methylation.
REACT_261168. Aflatoxin activation and detoxification.
SABIO-RK P04799.

Miscellaneous databases

NextBioi 602906.
PROi P04799.

Gene expression databases

ExpressionAtlasi P04799. baseline.
Genevestigatori P04799.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Coding nucleotide sequence of 3-methylcholanthrene-inducible cytochrome P-450d cDNA from rat liver."
    Kawajiri K., Gotoh O., Sogawa K., Tagashira Y., Muramatsu M., Fujii-Kuriyama Y.
    Proc. Natl. Acad. Sci. U.S.A. 81:1649-1653(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete nucleotide sequence of a methylcholanthrene-inducible cytochrome P-450 (P-450d) gene in the rat."
    Sogawa K., Gotoh O., Kawajiri K., Harada T., Fujii-Kuriyama Y.
    J. Biol. Chem. 260:5026-5032(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Stable expression of rat cytochrome P450IA2 cDNA and hydroxylation of 17 beta-estradiol and 2-aminofluorene in V79 Chinese hamster cells."
    Wolfel C., Platt K.L., Dogra S., Glatt H., Wachter F., Doehmer J.
    Mol. Carcinog. 4:489-498(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "The primary structure of cytochrome P-450d purified from rat liver microsomes: prediction of helical regions and domain analysis."
    Haniu M., Ryan D.E., Levin W., Shively J.E.
    Arch. Biochem. Biophys. 244:323-337(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  6. "Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450."
    Cheng K.C., Park S.S., Krutzsch H.C., Grantham P.H., Gelboin H.V., Friedman F.K.
    Biochemistry 25:2397-2402(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-26.
  7. "Amino-terminal and carboxy-terminal sequence of hepatic microsomal cytochrome P-450d, a unique hemoprotein from rats treated with isosafrole."
    Botelho L.H., Ryan D.E., Yuan P.M., Kutny R., Shively J.E., Levin W.
    Biochemistry 21:1152-1155(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-20.
  8. "Modification of cytochrome P450 1A2 enzymes by the mechanism-based inactivator 2-ethynylnaphthalene and the photoaffinity label 4-azidobiphenyl."
    Yun C.H., Hammons G.J., Jones G., Martin M.V., Hopkins N.E., Alworth W.L., Guengerich F.P.
    Biochemistry 31:10556-10563(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 67-78.
  9. "Characterization of complementary DNA clones coding for two forms of 3-methylcholanthrene-inducible rat liver cytochrome P-450."
    Yabusaki Y., Murakami H., Nakamura K., Nomura N., Shimizu M., Oeda K., Ohkawa H.
    J. Biochem. 96:793-804(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 147-513.
  10. "Discovery and confirmation of O-GlcNAcylated proteins in rat liver mitochondria by combination of mass spectrometry and immunological methods."
    Cao W., Cao J., Huang J., Yao J., Yan G., Xu H., Yang P.
    PLoS ONE 8:E76399-E76399(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-68.

Entry informationi

Entry nameiCP1A2_RAT
AccessioniPrimary (citable) accession number: P04799
Secondary accession number(s): A1L120, Q64588
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: May 1, 2007
Last modified: November 26, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3