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Protein

Cytochrome P450 1A2

Gene

Cyp1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation.By similarity

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi456 – 4561Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.14.1. 5301.
ReactomeiR-RNO-156581. Methylation.
R-RNO-211957. Aromatic amines can be N-hydroxylated or N-dealkylated by CYP1A2.
R-RNO-2142670. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
R-RNO-2142816. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
R-RNO-5423646. Aflatoxin activation and detoxification.
SABIO-RKP04799.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1By similarity)
Alternative name(s):
CYPIA2
Cholesterol 25-hydroxylaseBy similarity
Cytochrome P-448
Cytochrome P-450d
Cytochrome P450-D
Gene namesi
Name:Cyp1a2
Synonyms:Cyp1a-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2459. Cyp1a2.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1075125.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 513512Cytochrome P450 1A2PRO_0000051656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi68 – 681O-linked (GlcNAc)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP04799.
PRIDEiP04799.

PTM databases

iPTMnetiP04799.
PhosphoSiteiP04799.

Expressioni

Inductioni

By 3-methylcholanthrene (3MC).

Gene expression databases

BgeeiENSRNOG00000016173.
GenevisibleiP04799. RN.

Interactioni

Protein-protein interaction databases

BioGridi246478. 1 interaction.
MINTiMINT-4564487.
STRINGi10116.ENSRNOP00000021653.

Chemistry

BindingDBiP04799.

Structurei

3D structure databases

ProteinModelPortaliP04799.
SMRiP04799. Positions 33-512.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118992.
HOVERGENiHBG106944.
InParanoidiP04799.
KOiK07409.
OMAiPRKSEEM.
OrthoDBiEOG091G0BT8.
PhylomeDBiP04799.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04799-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFSQYISLA PELLLATAIF CLVFWVLRGT RTQVPKGLKS PPGPWGLPFI
60 70 80 90 100
GHMLTLGKNP HLSLTKLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVKQ
110 120 130 140 150
GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS
160 170 180 190 200
DPTSVSSCYL EEHVSKEANH LISKFQKLMA EVGHFEPVNQ VVESVANVIG
210 220 230 240 250
AMCFGKNFPR KSEEMLNLVK SSKDFVENVT SGNAVDFFPV LRYLPNPALK
260 270 280 290 300
RFKNFNDNFV LFLQKTVQEH YQDFNKNSIQ DITGALFKHS ENYKDNGGLI
310 320 330 340 350
PQEKIVNIVN DIFGAGFETV TTAIFWSILL LVTEPKVQRK IHEELDTVIG
360 370 380 390 400
RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP
410 420 430 440 450
KECCIFINQW QVNHDEKQWK DPFVFRPERF LTNDNTAIDK TLSEKVMLFG
460 470 480 490 500
LGKRRCIGEI PAKWEVFLFL AILLHQLEFT VPPGVKVDLT PSYGLTMKPR
510
TCEHVQAWPR FSK
Length:513
Mass (Da):58,259
Last modified:May 1, 2007 - v2
Checksum:iB1F06FDE9B7B6FA7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261V → M AA sequence (PubMed:3718958).Curated
Sequence conflicti137 – 1371R → H in AAA41053 (PubMed:2985574).Curated
Sequence conflicti137 – 1371R → H no nucleotide entry (PubMed:1793487).Curated
Sequence conflicti185 – 1851F → L no nucleotide entry (PubMed:1793487).Curated
Sequence conflicti262 – 2621F → S in AAA41028 (PubMed:6584898).Curated
Sequence conflicti403 – 4031C → R in AAA41053 (PubMed:2985574).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02422 mRNA. Translation: AAA41028.1.
K03241 Genomic DNA. Translation: AAA41053.1.
BC127476 mRNA. Translation: AAI27477.1.
X01031 mRNA. Translation: CAA25516.1.
PIRiA61400.
RefSeqiNP_036673.3. NM_012541.3.
UniGeneiRn.5563.

Genome annotation databases

EnsembliENSRNOT00000021653; ENSRNOP00000021653; ENSRNOG00000016173.
GeneIDi24297.
KEGGirno:24297.
UCSCiRGD:2459. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02422 mRNA. Translation: AAA41028.1.
K03241 Genomic DNA. Translation: AAA41053.1.
BC127476 mRNA. Translation: AAI27477.1.
X01031 mRNA. Translation: CAA25516.1.
PIRiA61400.
RefSeqiNP_036673.3. NM_012541.3.
UniGeneiRn.5563.

3D structure databases

ProteinModelPortaliP04799.
SMRiP04799. Positions 33-512.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246478. 1 interaction.
MINTiMINT-4564487.
STRINGi10116.ENSRNOP00000021653.

Chemistry

BindingDBiP04799.
ChEMBLiCHEMBL1075125.

PTM databases

iPTMnetiP04799.
PhosphoSiteiP04799.

Proteomic databases

PaxDbiP04799.
PRIDEiP04799.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021653; ENSRNOP00000021653; ENSRNOG00000016173.
GeneIDi24297.
KEGGirno:24297.
UCSCiRGD:2459. rat.

Organism-specific databases

CTDi1544.
RGDi2459. Cyp1a2.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118992.
HOVERGENiHBG106944.
InParanoidiP04799.
KOiK07409.
OMAiPRKSEEM.
OrthoDBiEOG091G0BT8.
PhylomeDBiP04799.

Enzyme and pathway databases

BRENDAi1.14.14.1. 5301.
ReactomeiR-RNO-156581. Methylation.
R-RNO-211957. Aromatic amines can be N-hydroxylated or N-dealkylated by CYP1A2.
R-RNO-2142670. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
R-RNO-2142816. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
R-RNO-5423646. Aflatoxin activation and detoxification.
SABIO-RKP04799.

Miscellaneous databases

PROiP04799.

Gene expression databases

BgeeiENSRNOG00000016173.
GenevisibleiP04799. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP1A2_RAT
AccessioniPrimary (citable) accession number: P04799
Secondary accession number(s): A1L120, Q64588
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: May 1, 2007
Last modified: September 7, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.