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P04799

- CP1A2_RAT

UniProt

P04799 - CP1A2_RAT

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Protein
Cytochrome P450 1A2
Gene
Cyp1a2, Cyp1a-2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi456 – 4561Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. caffeine oxidase activity Source: Ensembl
  3. demethylase activity Source: Ensembl
  4. heme binding Source: UniProtKB
  5. iron ion binding Source: InterPro
  6. monooxygenase activity Source: RGD
  7. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: RGD
Complete GO annotation...

GO - Biological processi

  1. alkaloid metabolic process Source: Ensembl
  2. cellular respiration Source: Ensembl
  3. cellular response to cadmium ion Source: Ensembl
  4. dibenzo-p-dioxin metabolic process Source: Ensembl
  5. exogenous drug catabolic process Source: Ensembl
  6. hydrogen peroxide biosynthetic process Source: Ensembl
  7. lung development Source: Ensembl
  8. monocarboxylic acid metabolic process Source: Ensembl
  9. monoterpenoid metabolic process Source: Ensembl
  10. oxidative deethylation Source: Ensembl
  11. oxidative demethylation Source: Ensembl
  12. porphyrin-containing compound metabolic process Source: Ensembl
  13. post-embryonic development Source: Ensembl
  14. regulation of gene expression Source: Ensembl
  15. response to drug Source: RGD
  16. response to estradiol Source: RGD
  17. response to immobilization stress Source: RGD
  18. response to lipopolysaccharide Source: RGD
  19. response to organic cyclic compound Source: RGD
  20. response to organic substance Source: RGD
  21. steroid catabolic process Source: Ensembl
  22. toxin biosynthetic process Source: Ensembl
  23. xenobiotic metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198836. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
REACT_198844. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
SABIO-RKP04799.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 1A2 (EC:1.14.14.1)
Alternative name(s):
CYPIA2
Cytochrome P-448
Cytochrome P-450d
Cytochrome P450-D
Gene namesi
Name:Cyp1a2
Synonyms:Cyp1a-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi2459. Cyp1a2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 513512Cytochrome P450 1A2
PRO_0000051656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi68 – 681O-linked (GlcNAc)

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP04799.

Expressioni

Inductioni

By 3-methylcholanthrene (3MC).

Gene expression databases

GenevestigatoriP04799.

Interactioni

Protein-protein interaction databases

BioGridi246478. 1 interaction.
MINTiMINT-4564487.

Structurei

3D structure databases

ProteinModelPortaliP04799.
SMRiP04799. Positions 33-512.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Phylogenomic databases

GeneTreeiENSGT00750000117317.
HOVERGENiHBG106944.
KOiK07409.
OMAiAFILEIY.
OrthoDBiEOG7RBZ85.
PhylomeDBiP04799.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04799-1 [UniParc]FASTAAdd to Basket

« Hide

MAFSQYISLA PELLLATAIF CLVFWVLRGT RTQVPKGLKS PPGPWGLPFI    50
GHMLTLGKNP HLSLTKLSQQ YGDVLQIRIG STPVVVLSGL NTIKQALVKQ 100
GDDFKGRPDL YSFTLITNGK SMTFNPDSGP VWAARRRLAQ DALKSFSIAS 150
DPTSVSSCYL EEHVSKEANH LISKFQKLMA EVGHFEPVNQ VVESVANVIG 200
AMCFGKNFPR KSEEMLNLVK SSKDFVENVT SGNAVDFFPV LRYLPNPALK 250
RFKNFNDNFV LFLQKTVQEH YQDFNKNSIQ DITGALFKHS ENYKDNGGLI 300
PQEKIVNIVN DIFGAGFETV TTAIFWSILL LVTEPKVQRK IHEELDTVIG 350
RDRQPRLSDR PQLPYLEAFI LEIYRYTSFV PFTIPHSTTR DTSLNGFHIP 400
KECCIFINQW QVNHDEKQWK DPFVFRPERF LTNDNTAIDK TLSEKVMLFG 450
LGKRRCIGEI PAKWEVFLFL AILLHQLEFT VPPGVKVDLT PSYGLTMKPR 500
TCEHVQAWPR FSK 513
Length:513
Mass (Da):58,259
Last modified:May 1, 2007 - v2
Checksum:iB1F06FDE9B7B6FA7
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261V → M AA sequence 1 Publication
Sequence conflicti137 – 1371R → H in AAA41053. 1 Publication
Sequence conflicti137 – 1371R → H no nucleotide entry 1 Publication
Sequence conflicti185 – 1851F → L no nucleotide entry 1 Publication
Sequence conflicti262 – 2621F → S in AAA41028. 1 Publication
Sequence conflicti403 – 4031C → R in AAA41053. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02422 mRNA. Translation: AAA41028.1.
K03241 Genomic DNA. Translation: AAA41053.1.
BC127476 mRNA. Translation: AAI27477.1.
X01031 mRNA. Translation: CAA25516.1.
PIRiA61400.
RefSeqiNP_036673.3. NM_012541.3.
UniGeneiRn.5563.

Genome annotation databases

EnsembliENSRNOT00000021653; ENSRNOP00000021653; ENSRNOG00000016173.
GeneIDi24297.
KEGGirno:24297.
UCSCiRGD:2459. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02422 mRNA. Translation: AAA41028.1 .
K03241 Genomic DNA. Translation: AAA41053.1 .
BC127476 mRNA. Translation: AAI27477.1 .
X01031 mRNA. Translation: CAA25516.1 .
PIRi A61400.
RefSeqi NP_036673.3. NM_012541.3.
UniGenei Rn.5563.

3D structure databases

ProteinModelPortali P04799.
SMRi P04799. Positions 33-512.
ModBasei Search...

Protein-protein interaction databases

BioGridi 246478. 1 interaction.
MINTi MINT-4564487.

Chemistry

ChEMBLi CHEMBL1075125.

Proteomic databases

PRIDEi P04799.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000021653 ; ENSRNOP00000021653 ; ENSRNOG00000016173 .
GeneIDi 24297.
KEGGi rno:24297.
UCSCi RGD:2459. rat.

Organism-specific databases

CTDi 1544.
RGDi 2459. Cyp1a2.

Phylogenomic databases

GeneTreei ENSGT00750000117317.
HOVERGENi HBG106944.
KOi K07409.
OMAi AFILEIY.
OrthoDBi EOG7RBZ85.
PhylomeDBi P04799.

Enzyme and pathway databases

Reactomei REACT_198836. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
REACT_198844. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).
SABIO-RK P04799.

Miscellaneous databases

NextBioi 602906.
PROi P04799.

Gene expression databases

Genevestigatori P04799.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008066. Cyt_P450_E_grp-I_CYP1.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01683. EP450ICYP1A.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Coding nucleotide sequence of 3-methylcholanthrene-inducible cytochrome P-450d cDNA from rat liver."
    Kawajiri K., Gotoh O., Sogawa K., Tagashira Y., Muramatsu M., Fujii-Kuriyama Y.
    Proc. Natl. Acad. Sci. U.S.A. 81:1649-1653(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete nucleotide sequence of a methylcholanthrene-inducible cytochrome P-450 (P-450d) gene in the rat."
    Sogawa K., Gotoh O., Kawajiri K., Harada T., Fujii-Kuriyama Y.
    J. Biol. Chem. 260:5026-5032(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Stable expression of rat cytochrome P450IA2 cDNA and hydroxylation of 17 beta-estradiol and 2-aminofluorene in V79 Chinese hamster cells."
    Wolfel C., Platt K.L., Dogra S., Glatt H., Wachter F., Doehmer J.
    Mol. Carcinog. 4:489-498(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "The primary structure of cytochrome P-450d purified from rat liver microsomes: prediction of helical regions and domain analysis."
    Haniu M., Ryan D.E., Levin W., Shively J.E.
    Arch. Biochem. Biophys. 244:323-337(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  6. "Amino-terminal sequence and structure of monoclonal antibody immunopurified cytochromes P-450."
    Cheng K.C., Park S.S., Krutzsch H.C., Grantham P.H., Gelboin H.V., Friedman F.K.
    Biochemistry 25:2397-2402(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-26.
  7. "Amino-terminal and carboxy-terminal sequence of hepatic microsomal cytochrome P-450d, a unique hemoprotein from rats treated with isosafrole."
    Botelho L.H., Ryan D.E., Yuan P.M., Kutny R., Shively J.E., Levin W.
    Biochemistry 21:1152-1155(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-20.
  8. "Modification of cytochrome P450 1A2 enzymes by the mechanism-based inactivator 2-ethynylnaphthalene and the photoaffinity label 4-azidobiphenyl."
    Yun C.H., Hammons G.J., Jones G., Martin M.V., Hopkins N.E., Alworth W.L., Guengerich F.P.
    Biochemistry 31:10556-10563(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 67-78.
  9. "Characterization of complementary DNA clones coding for two forms of 3-methylcholanthrene-inducible rat liver cytochrome P-450."
    Yabusaki Y., Murakami H., Nakamura K., Nomura N., Shimizu M., Oeda K., Ohkawa H.
    J. Biochem. 96:793-804(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 147-513.
  10. "Discovery and confirmation of O-GlcNAcylated proteins in rat liver mitochondria by combination of mass spectrometry and immunological methods."
    Cao W., Cao J., Huang J., Yao J., Yan G., Xu H., Yang P.
    PLoS ONE 8:E76399-E76399(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-68.

Entry informationi

Entry nameiCP1A2_RAT
AccessioniPrimary (citable) accession number: P04799
Secondary accession number(s): A1L120, Q64588
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: May 1, 2007
Last modified: September 3, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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