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Protein

Triosephosphate isomerase, glycosomal

Gene
N/A
Organism
Trypanosoma brucei brucei
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

The enzyme contains a high proportion of positively-charged residues in beta-barrels V and VII (compared to the homologous regions in other triose isomerase sequences). Since 2 clusters of positive charges located at precise distances on the molecular surface are common to 4 glycosomal enzymes, it has been speculated that this might represent a signal for entry into glycosomes (PubMed:3015595).1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase, glycosomal
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11Substrate1
Binding sitei13Substrate1
Active sitei95Electrophile1
Active sitei167Proton acceptor1

GO - Molecular functioni

  • triose-phosphate isomerase activity Source: GeneDB

GO - Biological processi

  • gluconeogenesis Source: UniProtKB-UniPathway
  • glycolytic process Source: GeneDB

Keywordsi

Molecular functionIsomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

BRENDAi5.3.1.1 6519
SABIO-RKP04789
UniPathwayiUPA00109; UER00189
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase, glycosomal (EC:5.3.1.1)
Short name:
TIM
Short name:
Triose-phosphate isomerase
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Subcellular locationi

GO - Cellular componenti

  • glycosome Source: GeneDB

Keywords - Cellular componenti

Glycosome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000901421 – 250Triosephosphate isomerase, glycosomalAdd BLAST250

Proteomic databases

PRIDEiP04789

Interactioni

Subunit structurei

Homodimer.3 Publications

Chemistry databases

BindingDBiP04789

Structurei

Secondary structure

1250
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Beta strandi14 – 16Combined sources3
Helixi18 – 29Combined sources12
Beta strandi38 – 42Combined sources5
Helixi45 – 47Combined sources3
Helixi48 – 54Combined sources7
Beta strandi60 – 65Combined sources6
Beta strandi68 – 70Combined sources3
Helixi74 – 79Combined sources6
Helixi80 – 85Combined sources6
Beta strandi90 – 94Combined sources5
Helixi96 – 101Combined sources6
Helixi106 – 119Combined sources14
Beta strandi122 – 127Combined sources6
Helixi131 – 135Combined sources5
Helixi139 – 152Combined sources14
Helixi156 – 161Combined sources6
Beta strandi162 – 167Combined sources6
Helixi169 – 171Combined sources3
Beta strandi172 – 175Combined sources4
Helixi180 – 197Combined sources18
Helixi200 – 205Combined sources6
Beta strandi208 – 213Combined sources6
Turni216 – 218Combined sources3
Helixi219 – 223Combined sources5
Beta strandi224 – 227Combined sources4
Beta strandi230 – 234Combined sources5
Helixi235 – 238Combined sources4
Helixi242 – 247Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AG1X-ray2.36O/T1-250[»]
1DKWX-ray2.65A/B2-250[»]
1IIGX-ray2.60A/B1-250[»]
1IIHX-ray2.20A/B1-250[»]
1KV5X-ray1.65A/B1-250[»]
1ML1X-ray2.60A/C/E/G/I/K1-250[»]
1MSSX-ray2.40A/B1-250[»]
1MTMmodel-A1-250[»]
1TPDX-ray2.10A/B1-250[»]
1TPEX-ray2.10A1-250[»]
1TPFX-ray1.80A/B1-250[»]
1TRDX-ray2.50A/B1-250[»]
1TRIX-ray2.40A1-250[»]
1TSIX-ray2.84A/B1-250[»]
1TTIX-ray2.40A1-250[»]
1TTJX-ray2.40A1-250[»]
2J24X-ray2.10A/B1-250[»]
2J27X-ray1.15A/B1-250[»]
2V0TX-ray2.20A/B/C/D/E/F/G/H1-250[»]
2V2CX-ray1.89A1-250[»]
2V2DX-ray2.30A1-250[»]
2V2HX-ray1.18A/B/C1-250[»]
2V5LX-ray2.40A/B1-250[»]
2VEIX-ray1.89A/B/C2-250[»]
2VEKX-ray1.60A/B2-250[»]
2VELX-ray2.30A/B2-250[»]
2VEMX-ray2.20A/B2-250[»]
2VENX-ray2.00A/B2-250[»]
2WSQX-ray2.10A/B/C/D2-67[»]
A/B/C/D84-250[»]
2WSRX-ray1.65A2-67[»]
A84-250[»]
2X16X-ray2.13A/B2-250[»]
2X1RX-ray1.98A/B2-250[»]
2X1SX-ray1.93A/B2-250[»]
2X1TX-ray1.83A/B2-250[»]
2X1UX-ray1.84A/B2-250[»]
2X2GX-ray1.90A/B2-250[»]
2Y6ZX-ray2.60A1-250[»]
2Y70X-ray2.30A/B/C/D1-250[»]
3Q37X-ray1.65A/B/C/D2-35[»]
A/B/C/D92-119[»]
3TIMX-ray2.80A/B1-250[»]
4JEQX-ray2.30A/B/C/D/E/F/G/H/I/J/K/L1-250[»]
4PC8X-ray1.55A1-250[»]
4PCFX-ray2.71A/B/C1-250[»]
4TIMX-ray2.40A/B1-250[»]
5I3FX-ray1.72A/B/C/D1-250[»]
5I3GX-ray1.96A/B/C/D1-250[»]
5I3HX-ray2.25A/B1-250[»]
5I3IX-ray2.20A/B/C/D1-250[»]
5I3JX-ray1.80A/B1-250[»]
5I3KX-ray2.21A/B/C/D1-250[»]
5TIMX-ray1.83A/B1-250[»]
6TIMX-ray2.20A/B1-250[»]
ProteinModelPortaliP04789
SMRiP04789
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04789

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Family and domain databases

CDDicd00311 TIM, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00147_B TIM_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035990 TIM_sf
IPR022896 TrioseP_Isoase_bac/euk
IPR000652 Triosephosphate_isomerase
IPR020861 Triosephosphate_isomerase_AS
PANTHERiPTHR21139 PTHR21139, 1 hit
PfamiView protein in Pfam
PF00121 TIM, 1 hit
SUPFAMiSSF51351 SSF51351, 1 hit
TIGRFAMsiTIGR00419 tim, 1 hit
PROSITEiView protein in PROSITE
PS00171 TIM_1, 1 hit
PS51440 TIM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P04789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPQPIAAA NWKCNGSQQS LSELIDLFNS TSINHDVQCV VASTFVHLAM
60 70 80 90 100
TKERLSHPKF VIAAQNAIAK SGAFTGEVSL PILKDFGVNW IVLGHSERRA
110 120 130 140 150
YYGETNEIVA DKVAAAVASG FMVIACIGET LQERESGRTA VVVLTQIAAI
160 170 180 190 200
AKKLKKADWA KVVIAYEPVW AIGTGKVATP QQAQEAHALI RSWVSSKIGA
210 220 230 240 250
DVAGELRILY GGSVNGKNAR TLYQQRDVNG FLVGGASLKP EFVDIIKATQ
Length:250
Mass (Da):26,835
Last modified:February 1, 1995 - v2
Checksum:i189D7A00632040FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03921 Genomic DNA Translation: CAA27559.1

Genome annotation databases

GeneDBiTb927.11.5520:pep

Similar proteinsi

Entry informationi

Entry nameiTPIS_TRYBB
AccessioniPrimary (citable) accession number: P04789
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

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