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P04775 (SCN2A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sodium channel protein type 2 subunit alpha
Alternative name(s):
Sodium channel protein brain II subunit alpha
Sodium channel protein type II subunit alpha
Voltage-gated sodium channel subunit alpha Nav1.2
Gene names
Name:Scn2a
Synonyms:Scn2a1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length2005 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient.

Subunit structure

The sodium channel consists of a large polypeptide and 2-3 smaller ones. This sequence represents a large polypeptide. Interacts with NEDD4L. Ref.2

Subcellular location

Membrane; Multi-pass membrane protein.

Domain

The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

Post-translational modification

May be ubiquitinated by NEDD4L; which would promote its endocytosis.

Sequence similarities

Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.2/SCN2A subfamily. [View classification]

Contains 1 IQ domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Syt1P217072EBI-2619448,EBI-458098

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20052005Sodium channel protein type 2 subunit alpha
PRO_0000048492

Regions

Transmembrane125 – 14824Helical; Name=S1 of repeat I; Potential
Transmembrane157 – 17620Helical; Name=S2 of repeat I; Potential
Transmembrane190 – 20819Helical; Name=S3 of repeat I; Potential
Transmembrane215 – 23420Helical; Voltage-sensor; Name=S4 of repeat I; Potential
Transmembrane251 – 27424Helical; Name=S5 of repeat I; Potential
Transmembrane402 – 42726Helical; Name=S6 of repeat I; Potential
Transmembrane754 – 77825Helical; Name=S1 of repeat II; Potential
Transmembrane790 – 81324Helical; Name=S2 of repeat II; Potential
Transmembrane822 – 84120Helical; Name=S3 of repeat II; Potential
Transmembrane848 – 86720Helical; Voltage-sensor; Name=S4 of repeat II; Potential
Transmembrane884 – 90421Helical; Name=S5 of repeat II; Potential
Transmembrane958 – 98326Helical; Name=S6 of repeat II; Potential
Transmembrane1204 – 122724Helical; Name=S1 of repeat III; Potential
Transmembrane1241 – 126626Helical; Name=S2 of repeat III; Potential
Transmembrane1273 – 129422Helical; Name=S3 of repeat III; Potential
Transmembrane1299 – 132022Helical; Voltage-sensor; Name=S4 of repeat III; Potential
Transmembrane1340 – 136728Helical; Name=S5 of repeat III; Potential
Transmembrane1447 – 147327Helical; Name=S6 of repeat III; Potential
Transmembrane1527 – 155024Helical; Name=S1 of repeat IV; Potential
Transmembrane1562 – 158524Helical; Name=S2 of repeat IV; Potential
Transmembrane1592 – 161524Helical; Name=S3 of repeat IV; Potential
Transmembrane1626 – 164722Helical; Voltage-sensor; Name=S4 of repeat IV; Potential
Transmembrane1663 – 168523Helical; Name=S5 of repeat IV; Potential
Transmembrane1752 – 177625Helical; Name=S6 of repeat IV; Potential
Repeat111 – 456346I
Repeat741 – 1013273II
Repeat1190 – 1504315III
Repeat1513 – 1811299IV
Domain1905 – 193430IQ

Amino acid modifications

Glycosylation2121N-linked (GlcNAc...) Potential
Glycosylation2851N-linked (GlcNAc...) Potential
Glycosylation2911N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation3081N-linked (GlcNAc...) Potential
Glycosylation3401N-linked (GlcNAc...) Potential
Glycosylation6041N-linked (GlcNAc...) Potential
Glycosylation6241N-linked (GlcNAc...) Potential
Glycosylation8831N-linked (GlcNAc...) Potential
Glycosylation10551N-linked (GlcNAc...) Potential
Glycosylation10721N-linked (GlcNAc...) Potential
Glycosylation11361N-linked (GlcNAc...) Potential
Glycosylation13681N-linked (GlcNAc...) Potential
Glycosylation13821N-linked (GlcNAc...) Potential
Glycosylation13931N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis19751Y → A: Abolishes interaction with NEDD4L. Ref.2

Secondary structure

1 ... 2005
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04775 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 861BE583D79F8324

FASTA2,005227,874
        10         20         30         40         50         60 
MARSVLVPPG PDSFRFFTRE SLAAIEQRIA EEKAKRPKQE RKDEDDENGP KPNSDLEAGK 

        70         80         90        100        110        120 
SLPFIYGDIP PEMVSEPLED LDPYYINKKT FIVLNKGKAI SRFSATSALY ILTPFNPIRK 

       130        140        150        160        170        180 
LAIKILVHSL FNVLIMCTIL TNCVFMTMSN PPDWTKNVEY TFTGIYTFES LIKILARGFC 

       190        200        210        220        230        240 
LEDFTFLRNP WNWLDFTVIT FAYVTEFVNL GNVSALRTFR VLRALKTISV IPGLKTIVGA 

       250        260        270        280        290        300 
LIQSVKKLSD VMILTVFCLS VFALIGLQLF MGNLRNKCLQ WPPDNSTFEI NITSFFNNSL 

       310        320        330        340        350        360 
DWNGTAFNRT VNMFNWDEYI EDKSHFYFLE GQNDALLCGN SSDAGQCPEG YICVKAGRNP 

       370        380        390        400        410        420 
NYGYTSFDTF SWAFLSLFRL MTQDFWENLY QLTLRAAGKT YMIFFVLVIF LGSFYLINLI 

       430        440        450        460        470        480 
LAVVAMAYEE QNQATLEEAE QKEAEFQQML EQLKKQQEEA QAAAAAASAE SRDFSGAGGI 

       490        500        510        520        530        540 
GVFSESSSVA SKLSSKSEKE LKNRRKKKKQ KEQAGEEEKE DAVRKSASED SIRKKGFQFS 

       550        560        570        580        590        600 
LEGSRLTYEK RFSSPHQSLL SIRGSLFSPR RNSRASLFNF KGRVKDIGSE NDFADDEHST 

       610        620        630        640        650        660 
FEDNDSRRDS LFVPHRHGER RPSNVSQASR ASRGIPTLPM NGKMHSAVDC NGVVSLVGGP 

       670        680        690        700        710        720 
SALTSPVGQL LPEGTTTETE IRKRRSSSYH VSMDLLEDPS RQRAMSMASI LTNTMEELEE 

       730        740        750        760        770        780 
SRQKCPPCWY KFANMCLIWD CCKPWLKVKH VVNLVVMDPF VDLAITICIV LNTLFMAMEH 

       790        800        810        820        830        840 
YPMTEQFSSV LSVGNLVFTG IFTAEMFLKI IAMDPYYYFQ EGWNIFDGFI VSLSLMELGL 

       850        860        870        880        890        900 
ANVEGLSVLR SFRLLRVFKL AKSWPTLNML IKIIGNSVGA LGNLTLVLAI IVFIFAVVGM 

       910        920        930        940        950        960 
QLFGKSYKEC VCKISNDCEL PRWHMHHFFH SFLIVFRVLC GEWIETMWDC MEVAGQTMCL 

       970        980        990       1000       1010       1020 
TVFMMVMVIG NLVVLNLFLA LLLSSFSSDN LAATDDDNEM NNLQIAVGRM QKGIDFVKRK 

      1030       1040       1050       1060       1070       1080 
IREFIQKAFV RKQKALDEIK PLEDLNNKKD SCISNHTTIE IGKDLNYLKD GNGTTSGIGS 

      1090       1100       1110       1120       1130       1140 
SVEKYVVDES DYMSFINNPS LTVTVPIALG ESDFENLNTE EFSSESDMEE SKEKLNATSS 

      1150       1160       1170       1180       1190       1200 
SEGSTVDIGA PAEGEQPEAE PEESLEPEAC FTEDCVRKFK CCQISIEEGK GKLWWNLRKT 

      1210       1220       1230       1240       1250       1260 
CYKIVEHNWF ETFIVFMILL SSGALAFEDI YIEQRKTIKT MLEYADKVFT YIFILEMLLK 

      1270       1280       1290       1300       1310       1320 
WVAYGFQMYF TNAWCWLDFL IVDVSLVSLT ANALGYSELG AIKSLRTLRA LRPLRALSRF 

      1330       1340       1350       1360       1370       1380 
EGMRVVVNAL LGAIPSIMNV LLVCLIFWLI FSIMGVNLFA GKFYHCINYT TGEMFDVSVV 

      1390       1400       1410       1420       1430       1440 
NNYSECQALI ESNQTARWKN VKVNFDNVGL GYLSLLQVAT FKGWMDIMYA AVDSRNVELQ 

      1450       1460       1470       1480       1490       1500 
PKYEDNLYMY LYFVIFIIFG SFFTLNLFIG VIIDNFNQQK KKFGGQDIFM TEEQKKYYNA 

      1510       1520       1530       1540       1550       1560 
MKKLGSKKPQ KPIPRPANKF QGMVFDFVTK QVFDISIMIL ICLNMVTMMV ETDDQSQEMT 

      1570       1580       1590       1600       1610       1620 
NILYWINLVF IVLFTGECVL KLISLRHYYF TIGWNIFDFV VVILSIVGMF LAELIEKYFV 

      1630       1640       1650       1660       1670       1680 
SPTLFRVIRL ARIGRILRLI KGAKGIRTLL FALMMSLPAL FNIGLLLFLV MFIYAIFGMS 

      1690       1700       1710       1720       1730       1740 
NFAYVKREVG IDDMFNFETF GNSMICLFQI TTSAGWDGLL APILNSGPPD CDPEKDHPGS 

      1750       1760       1770       1780       1790       1800 
SVKGDCGNPS VGIFFFVSYI IISFLVVVNM YIAVILENFS VATEESAEPL SEDDFEMFYE 

      1810       1820       1830       1840       1850       1860 
VWEKFDPDAT QFIEFCKLSD FAAALDPPLL IAKPNKVQLI AMDLPMVSGD RIHCLDILFA 

      1870       1880       1890       1900       1910       1920 
FTKRVLGESG EMDALRIQME ERFMASNPSK VSYEPITTTL KRKQEEVSAI VIQRAYRRYL 

      1930       1940       1950       1960       1970       1980 
LKQKVKKVSS IYKKDKGKED EGTPIKEDII TDKLNENSTP EKTDVTPSTT SPPSYDSVTK 

      1990       2000 
PEKEKFEKDK SEKEDKGKDI RESKK

« Hide

References

[1]"Existence of distinct sodium channel messenger RNAs in rat brain."
Noda M., Ikeda T., Kayano T., Suzuki H., Takeshima H., Kurasaki M., Takahashi H., Numa S.
Nature 320:188-192(1986) [PubMed: 3754035] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular determinants of voltage-gated sodium channel regulation by the Nedd4/Nedd4-like proteins."
Rougier J.-S., van Bemmelen M.X., Bruce M.C., Jespersen T., Gavillet B., Apotheloz F., Cordonier S., Staub O., Rotin D., Abriel H.
Am. J. Physiol. 288:C692-C701(2005) [PubMed: 15548568] [Abstract]
Cited for: INTERACTION WITH NEDD4L, POSSIBLE UBIQUITINATION, MUTAGENESIS OF TYR-1975.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X03639 mRNA. Translation: CAA27287.1.
IPIIPI00781886.
RefSeqNP_036779.1. NM_012647.1.
UniGeneRn.89192.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BYYNMR-A1474-1526[»]
2KXWNMR-B1901-1927[»]
ProteinModelPortalP04775.
ModBaseSearch...

Protein-protein interaction databases

IntActP04775. 1 interaction.
STRINGP04775.

PTM databases

PhosphoSiteP04775.

Proteomic databases

PRIDEP04775.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24766.
KEGGrno:24766.
UCSCNM_012647. rat.

Organism-specific databases

CTD110876.
RGD3632. Scn2a1.

Phylogenomic databases

eggNOGmaNOG14295.
HOVERGENHBG053100.
InParanoidP04775.

Gene expression databases

ArrayExpressP04775.
GenevestigatorP04775.
GermOnlineENSRNOG00000005018. Rattus norvegicus.

Family and domain databases

InterProIPR024583. DUF3451.
IPR005821. Ion_trans.
IPR000048. IQ_motif_EF-hand-BS.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view]
KOK04834.
PfamPF11933. DUF3451. 1 hit.
PF00520. Ion_trans. 4 hits.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view]
PRINTSPR00170. NACHANNEL.
SMARTSM00015. IQ. 1 hit.
[Graphical view]
PROSITEPS50096. IQ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604325.

Entry information

Entry nameSCN2A_RAT
AccessionPrimary (citable) accession number: P04775
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 16, 2011
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents