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P04774

- SCN1A_RAT

UniProt

P04774 - SCN1A_RAT

Protein

Sodium channel protein type 1 subunit alpha

Gene

Scn1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient.

    GO - Molecular functioni

    1. sodium ion binding Source: RGD
    2. voltage-gated sodium channel activity Source: RGD

    GO - Biological processi

    1. membrane depolarization during action potential Source: RefGenome
    2. neuronal action potential Source: RGD
    3. sodium ion transmembrane transport Source: GOC

    Keywords - Molecular functioni

    Ion channel, Sodium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium channel protein type 1 subunit alpha
    Alternative name(s):
    Sodium channel protein brain I subunit alpha
    Sodium channel protein type I subunit alpha
    Voltage-gated sodium channel subunit alpha Nav1.1
    Gene namesi
    Name:Scn1a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi69364. Scn1a.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: RGD
    2. voltage-gated sodium channel complex Source: RGD
    3. Z disc Source: BHF-UCL

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 20092009Sodium channel protein type 1 subunit alphaPRO_0000048490Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi338 – 3381N-linked (GlcNAc...)Sequence Analysis
    Modified residuei470 – 4701Phosphoserine1 Publication
    Modified residuei551 – 5511Phosphoserine1 Publication
    Modified residuei607 – 6071Phosphoserine1 Publication
    Glycosylationi1378 – 13781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1392 – 13921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1403 – 14031N-linked (GlcNAc...)Sequence Analysis
    Modified residuei1516 – 15161Phosphoserine; by PKCBy similarity

    Post-translational modificationi

    Phosphorylation at Ser-1516 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP04774.
    PRIDEiP04774.

    Expressioni

    Gene expression databases

    GenevestigatoriP04774.

    Interactioni

    Subunit structurei

    The sodium channel consists of a large polypeptide and 2-3 smaller ones. This sequence represents a large polypeptide. Interacts with FGF13; may regulate SCN1A activity By similarity.By similarity

    Protein-protein interaction databases

    BioGridi249530. 1 interaction.
    STRINGi10116.ENSRNOP00000051071.

    Structurei

    3D structure databases

    ProteinModelPortaliP04774.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 123123CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini148 – 1558ExtracellularSequence Analysis
    Topological domaini176 – 18813CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini208 – 2136ExtracellularSequence Analysis
    Topological domaini234 – 24916CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini274 – 399126ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini426 – 762337CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini788 – 79811ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini823 – 8308CytoplasmicSequence Analysis
    Topological domaini851 – 8566ExtracellularSequence Analysis
    Topological domaini877 – 89216CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini914 – 96653ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini993 – 1213221CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1238 – 125013ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1277 – 12826CytoplasmicSequence Analysis
    Topological domaini1305 – 13084ExtracellularSequence Analysis
    Topological domaini1331 – 134919CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1378 – 145679ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1484 – 153653CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1561 – 157111ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1596 – 16016CytoplasmicSequence Analysis
    Topological domaini1626 – 163510ExtracellularSequence Analysis
    Topological domaini1658 – 167215CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1696 – 176166ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1787 – 2009223CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei124 – 14724Helical; Name=S1 of repeat IAdd
    BLAST
    Transmembranei156 – 17520Helical; Name=S2 of repeat IAdd
    BLAST
    Transmembranei189 – 20719Helical; Name=S3 of repeat IAdd
    BLAST
    Transmembranei214 – 23320Helical; Voltage-sensor; Name=S4 of repeat IAdd
    BLAST
    Transmembranei250 – 27324Helical; Name=S5 of repeat IAdd
    BLAST
    Transmembranei400 – 42526Helical; Name=S6 of repeat IAdd
    BLAST
    Transmembranei763 – 78725Helical; Name=S1 of repeat IIAdd
    BLAST
    Transmembranei799 – 82224Helical; Name=S2 of repeat IIAdd
    BLAST
    Transmembranei831 – 85020Helical; Name=S3 of repeat IIAdd
    BLAST
    Transmembranei857 – 87620Helical; Voltage-sensor; Name=S4 of repeat IIAdd
    BLAST
    Transmembranei893 – 91321Helical; Name=S5 of repeat IIAdd
    BLAST
    Transmembranei967 – 99226Helical; Name=S6 of repeat IIAdd
    BLAST
    Transmembranei1214 – 123724Helical; Name=S1 of repeat IIIAdd
    BLAST
    Transmembranei1251 – 127626Helical; Name=S2 of repeat IIIAdd
    BLAST
    Transmembranei1283 – 130422Helical; Name=S3 of repeat IIIAdd
    BLAST
    Transmembranei1309 – 133022Helical; Voltage-sensor; Name=S4 of repeat IIIAdd
    BLAST
    Transmembranei1350 – 137728Helical; Name=S5 of repeat IIIAdd
    BLAST
    Transmembranei1457 – 148327Helical; Name=S6 of repeat IIIAdd
    BLAST
    Transmembranei1537 – 156024Helical; Name=S1 of repeat IVAdd
    BLAST
    Transmembranei1572 – 159524Helical; Name=S2 of repeat IVAdd
    BLAST
    Transmembranei1602 – 162524Helical; Name=S3 of repeat IVAdd
    BLAST
    Transmembranei1636 – 165722Helical; Voltage-sensor; Name=S4 of repeat IVAdd
    BLAST
    Transmembranei1673 – 169523Helical; Name=S5 of repeat IVAdd
    BLAST
    Transmembranei1762 – 178625Helical; Name=S6 of repeat IVAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati110 – 454345IAdd
    BLAST
    Repeati750 – 1022273IIAdd
    BLAST
    Repeati1200 – 1514315IIIAdd
    BLAST
    Repeati1523 – 1821299IVAdd
    BLAST
    Domaini1915 – 194430IQAdd
    BLAST

    Domaini

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Contains 1 IQ domain.Curated

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    HOGENOMiHOG000231755.
    HOVERGENiHBG053100.
    KOiK04833.
    PhylomeDBiP04774.

    Family and domain databases

    Gene3Di1.20.120.350. 4 hits.
    InterProiIPR027359. Channel_four-helix_dom.
    IPR024583. DUF3451.
    IPR005821. Ion_trans_dom.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR008051. Na_channel_a1su.
    IPR001696. Na_channel_asu.
    IPR010526. Na_trans_assoc.
    [Graphical view]
    PfamiPF11933. DUF3451. 1 hit.
    PF00520. Ion_trans. 4 hits.
    PF06512. Na_trans_assoc. 1 hit.
    [Graphical view]
    PRINTSiPR00170. NACHANNEL.
    PR01664. NACHANNEL1.
    SMARTiSM00015. IQ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04774-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQTVLVPPG PDSFNFFTRE SLAAIERRIA EEKAKNPKPD KKDDDENGPK     50
    PNSDLEAGKN LPFIYGDIPP EMVSEPLEDL DPYYINKKTF IVLNKGKAIF 100
    RFSATSALYI LTPFNPLRKI AIKILVHSLF SMLIMCTILT NCVFMTMSNP 150
    PDWTKNVEYT FTGIYTFESL IKIIARGFCL EDFTFLRDPW NWLDFTVITF 200
    AYVTEFVDLG NVSALRTFRV LRALKTISVI PGLKTIVGAL IQSVKKLSDV 250
    MILTVFCLSV FALIGLQLFM GNLRNKCVQW PPTNASLEEH SIEKNVTTDY 300
    NGTLVNETVF EFDWKSYIQD SRYHYFLEGV LDALLCGNSS DAGQCPEGYM 350
    CVKAGRNPNY GYTSFDTFSW AFLSLFRLMT QDFWENLYQL TLRAAGKTYM 400
    IFFVLVIFLG SFYLINLILA VVAMAYEEQN QATLEEAEQK EAEFQQMLEQ 450
    LKKQQEAAQQ AAAATASEHS REPSAAGRLS DSSSEASKLS SKSAKERRNR 500
    RKKRKQKEQS GGEEKDDDEF HKSESEDSIR RKGFRFSIEG NRLTYEKRYS 550
    SPHQSLLSIR GSLFSPRRNS RTSLFSFRGR AKDVGSENDF ADDEHSTFED 600
    NESRRDSLFV PRRHGERRNS NLSQTSRSSR MLAGLPANGK MHSTVDCNGV 650
    VSLVGGPSVP TSPVGQLLPE VIIDKPATDD NGTTTETEMR KRRSSSFHVS 700
    MDFLEDPSQR QRAMSIASIL TNTVEELEES RQKCPPCWYK FSNIFLIWDC 750
    SPYWLKVKHI VNLVVMDPFV DLAITICIVL NTLFMAMEHY PMTEHFNHVL 800
    TVGNLVFTGI FTAEMFLKII AMDPYYYFQE GWNIFDGFIV TLSLVELGLA 850
    NVEGLSVLRS FRLLRVFKLA KSWPTLNMLI KIIGNSVGAL GNLTLVLAII 900
    VFIFAVVGMQ LFGKSYKDCV CKIATDCKLP RWHMNDFFHS FLIVFRVLCG 950
    EWIETMWDCM EVAGQAMCLT VFMMVMVIRN LVVLNLFLAL LLSSFSADNL 1000
    AATDDDNEMN NLQIAVDRMH KGVAYVKRKI YEFIQQSFVR KQKILDEIKP 1050
    LDDLNNRKDN CTSNHTTEIG KDLDCLKDVN GTTSGIGTGS SVEKYIIDES 1100
    DYMSFINNPS LTVTVPIAVG ESDFENLNTE DFSSESDLEE SKEKLNESSS 1150
    SSEGSTVDIG APAEEQPVME PEETLEPEAC FTEGCVQRFK CCQISVEEGR 1200
    GKQWWNLRRT CFRIVEHNWF ETFIVFMILL SSGALAFEDI YIDQRKTIKT 1250
    MLEYADKVFT YIFILEMLLK WVAYGYQTYF TNAWCWLDFL IVDVSLVSLT 1300
    ANALGYSELG AIKSLRTLRA LRPLRALSRF EGMRVVVNAL LGAIPSIMNV 1350
    LLVCLIFWLI FSIMGVNLFA GKFYHCVNTT TGDTFEITEV NNHSDCLKLI 1400
    ERNETARWKN VKVNFDNVGF GYLSLLQVAT FKGWMDIMYA AVDSRNVELQ 1450
    PKYEESLYMY LYFVIFIIFG SFFTLNLFIG VIIDNFNQQK KKFGGQDIFM 1500
    TEEQKKYYNA MKKLGSKKPQ KPIPRPGNKF QGMVFDFVTR QVFDISIMIL 1550
    ICLNMVTMMV ETDDQSDYVT SILSRINLVF IVLFTGECVL KLISLRHYYF 1600
    TIGWNIFDFV VVILSIVGMF LAELIEKYFV SPTLFRVIRL ARIGRILRLI 1650
    KGAKGIRTLL FALMMSLPAL FNIGLLLFLV MFIYAIFGMS NFAYVKREVG 1700
    IDDMFNFETF GNSMICLFQI TTSAGWDGLL APILNSKPPD CDPNKVNPGS 1750
    SVKGDCGNPS VGIFFFVSYI IISFLVVVNM YIAVILENFS VATEESAEPL 1800
    SEDDFEMFYE VWEKFDPDAT QFMEFEKLSQ FAAALEPPLN LPQPNKLQLI 1850
    AMDLPMVSGD RIHCLDILFA FTKRVLGESG EMDALRIQME ERFMASNPSK 1900
    VSYQPITTTL KRKQEEVSAV IIQRAYRRHL LKRTVKQASF TYNKNKLKGG 1950
    ANLLVKEDMI IDRINENSIT EKTDLTMSTA ACPPSYDRVT KPIVEKHEQE 2000
    GKDEKAKGK 2009
    Length:2,009
    Mass (Da):228,770
    Last modified:August 13, 1987 - v1
    Checksum:i6808466F6368373B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03638 mRNA. Translation: CAA27286.1.
    M22253 mRNA. Translation: AAA79965.1.
    PIRiA25019.
    RefSeqiNP_110502.1. NM_030875.1.
    UniGeneiRn.32079.

    Genome annotation databases

    GeneIDi81574.
    KEGGirno:81574.
    UCSCiRGD:69364. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03638 mRNA. Translation: CAA27286.1 .
    M22253 mRNA. Translation: AAA79965.1 .
    PIRi A25019.
    RefSeqi NP_110502.1. NM_030875.1.
    UniGenei Rn.32079.

    3D structure databases

    ProteinModelPortali P04774.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249530. 1 interaction.
    STRINGi 10116.ENSRNOP00000051071.

    Chemistry

    BindingDBi P04774.
    ChEMBLi CHEMBL4906.
    GuidetoPHARMACOLOGYi 578.

    Proteomic databases

    PaxDbi P04774.
    PRIDEi P04774.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 81574.
    KEGGi rno:81574.
    UCSCi RGD:69364. rat.

    Organism-specific databases

    CTDi 6323.
    RGDi 69364. Scn1a.

    Phylogenomic databases

    eggNOGi COG1226.
    HOGENOMi HOG000231755.
    HOVERGENi HBG053100.
    KOi K04833.
    PhylomeDBi P04774.

    Miscellaneous databases

    NextBioi 615093.
    PROi P04774.

    Gene expression databases

    Genevestigatori P04774.

    Family and domain databases

    Gene3Di 1.20.120.350. 4 hits.
    InterProi IPR027359. Channel_four-helix_dom.
    IPR024583. DUF3451.
    IPR005821. Ion_trans_dom.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR008051. Na_channel_a1su.
    IPR001696. Na_channel_asu.
    IPR010526. Na_trans_assoc.
    [Graphical view ]
    Pfami PF11933. DUF3451. 1 hit.
    PF00520. Ion_trans. 4 hits.
    PF06512. Na_trans_assoc. 1 hit.
    [Graphical view ]
    PRINTSi PR00170. NACHANNEL.
    PR01664. NACHANNEL1.
    SMARTi SM00015. IQ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Existence of distinct sodium channel messenger RNAs in rat brain."
      Noda M., Ikeda T., Kayano T., Suzuki H., Takeshima H., Kurasaki M., Takahashi H., Numa S.
      Nature 320:188-192(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure and function of sodium channel."
      Noda M., Numa S.
      J. Recept. Res. 7:467-497(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Developmentally regulated alternative RNA splicing of rat brain sodium channel mRNAs."
      Sarao R., Gupta S.K., Auld V.J., Dunn R.J.
      Nucleic Acids Res. 19:5673-5679(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-253.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    4. "Multisite phosphorylation of voltage-gated sodium channel alpha subunits from rat brain."
      Berendt F.J., Park K.S., Trimmer J.S.
      J. Proteome Res. 9:1976-1984(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-470; SER-551 AND SER-607.

    Entry informationi

    Entry nameiSCN1A_RAT
    AccessioniPrimary (citable) accession number: P04774
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3