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Protein

Glutamine synthetase

Gene

GLUL

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity).By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Protein has several cofactor binding sites:

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase, Lyase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Short name:
GS
Alternative name(s):
Glutamate decarboxylase (EC:4.1.1.15)
Glutamate--ammonia ligase
Gene namesi
Name:GLUL
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 373372Glutamine synthetasePRO_0000153138Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei104 – 1041PhosphotyrosineBy similarity
Modified residuei343 – 3431PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated by ZNRF1.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Homooctamer and homotetramer. Interacts with PALMD (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP04773.
SMRiP04773. Positions 3-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

HOVERGENiHBG005847.
KOiK01915.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSASSHLN KGIKQMYMSL PQGEKVQAMY IWVDGTGEGL RCKTRTLDCE
60 70 80 90 100
PKCVEELPEW NFDGSSTFQS ESSNSDMYLS PVAMFRDPFR KEPNKLVFCE
110 120 130 140 150
VFKYNQKPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLL GTDGHPFGWP
160 170 180 190 200
SDGFPGPQGL YYCGVGADKA YRRDIMEAHY RACLYAGVKI TGTYAEVKHA
210 220 230 240 250
QWEFQIGPCE GIRMGDHLWV ARFILHRVCK DFGVIATFDS KPIPGNWNGA
260 270 280 290 300
GCHTNFSTKT MREENGLKHI KEAIEKLSKR HRYHIRAYDP KGGLDNARRL
310 320 330 340 350
TGFHKTSNIN DFSAGVADRS ASIRIPRTVG QEKKGYFEAR CPSANCDPFA
360 370
VTEAIVRTCL LNETGDQPFQ YKN
Length:373
Mass (Da):42,320
Last modified:January 23, 2007 - v4
Checksum:i611D58CE20FB16CF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121G → N in CAA27211 (Ref. 2) Curated
Sequence conflicti18 – 192MS → LC in CAA27211 (Ref. 2) Curated
Sequence conflicti72 – 721S → G in CAA27211 (Ref. 2) Curated
Sequence conflicti91 – 922KE → RD in CAA27211 (Ref. 2) Curated
Sequence conflicti106 – 1061Q → R in CAA27211 (Ref. 2) Curated
Sequence conflicti116 – 1161T → S in CAA27211 (Ref. 2) Curated
Sequence conflicti140 – 1401L → M in CAA27211 (Ref. 2) Curated
Sequence conflicti152 – 1521D → N in CAA27211 (Ref. 2) Curated
Sequence conflicti160 – 1601L → P in CAA27211 (Ref. 2) Curated
Sequence conflicti172 – 1721R → G in CAA27211 (Ref. 2) Curated
Sequence conflicti176 – 1761M → V in CAA27211 (Ref. 2) Curated
Sequence conflicti194 – 1941Y → N in CAA27211 (Ref. 2) Curated
Sequence conflicti198 – 1992KH → MP in CAA27211 (Ref. 2) Curated
Sequence conflicti230 – 2301K → E in CAA27211 (Ref. 2) Curated
Sequence conflicti240 – 2401S → P in CAA27211 (Ref. 2) Curated
Sequence conflicti260 – 2601T → A in CAA27211 (Ref. 2) Curated
Sequence conflicti271 – 2711K → E in CAA27211 (Ref. 2) Curated
Sequence conflicti299 – 2991R → G in CAA27211 (Ref. 2) Curated
Sequence conflicti305 – 3051K → E in CAA27211 (Ref. 2) Curated
Sequence conflicti318 – 3181D → N in CAA27211 (Ref. 2) Curated
Sequence conflicti339 – 3413ARC → DRR in CAA27211 (Ref. 2) Curated
Sequence conflicti367 – 3671Q → E in CAA27211 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03495 mRNA. Translation: CAA27211.1.
AF150961 mRNA. Translation: AAG43362.1.
RefSeqiNP_001233699.1. NM_001246770.1.

Genome annotation databases

GeneIDi100689337.
KEGGicge:100689337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03495 mRNA. Translation: CAA27211.1.
AF150961 mRNA. Translation: AAG43362.1.
RefSeqiNP_001233699.1. NM_001246770.1.

3D structure databases

ProteinModelPortaliP04773.
SMRiP04773. Positions 3-372.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100689337.
KEGGicge:100689337.

Organism-specific databases

CTDi2752.

Phylogenomic databases

HOVERGENiHBG005847.
KOiK01915.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The cloning and nucleotide sequence of cDNA for an amplified glutamine synthetase gene from the Chinese hamster."
    Hayward B.E., Hussain A., Wilson R.H., Lyons A., Woodcock V., McIntosh B., Harris T.J.R.
    Nucleic Acids Res. 14:999-1008(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.
  2. Tong Y., Wang H.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGLNA_CRIGR
AccessioniPrimary (citable) accession number: P04773
Secondary accession number(s): Q9EQP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 93 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.