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P04773 (GLNA_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase

Short name=GS
EC=6.3.1.2
Alternative name(s):
Glutamate decarboxylase
EC=4.1.1.15
Glutamate--ammonia ligase
Gene names
Name:GLUL
OrganismCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity.

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

L-glutamate = 4-aminobutanoate + CO2.

Cofactor

Biotin By similarity.

Magnesium or manganese By similarity.

Subunit structure

Homooctamer and homotetramer. Interacts with PALMD By similarity.

Subcellular location

Cytoplasm By similarity. Mitochondrion By similarity.

Post-translational modification

Ubiquitinated by ZNRF1 By similarity.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 373372Glutamine synthetase
PRO_0000153138

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1041Phosphotyrosine By similarity

Experimental info

Sequence conflict121G → N in CAA27211. Ref.2
Sequence conflict18 – 192MS → LC in CAA27211. Ref.2
Sequence conflict721S → G in CAA27211. Ref.2
Sequence conflict91 – 922KE → RD in CAA27211. Ref.2
Sequence conflict1061Q → R in CAA27211. Ref.2
Sequence conflict1161T → S in CAA27211. Ref.2
Sequence conflict1401L → M in CAA27211. Ref.2
Sequence conflict1521D → N in CAA27211. Ref.2
Sequence conflict1601L → P in CAA27211. Ref.2
Sequence conflict1721R → G in CAA27211. Ref.2
Sequence conflict1761M → V in CAA27211. Ref.2
Sequence conflict1941Y → N in CAA27211. Ref.2
Sequence conflict198 – 1992KH → MP in CAA27211. Ref.2
Sequence conflict2301K → E in CAA27211. Ref.2
Sequence conflict2401S → P in CAA27211. Ref.2
Sequence conflict2601T → A in CAA27211. Ref.2
Sequence conflict2711K → E in CAA27211. Ref.2
Sequence conflict2991R → G in CAA27211. Ref.2
Sequence conflict3051K → E in CAA27211. Ref.2
Sequence conflict3181D → N in CAA27211. Ref.2
Sequence conflict339 – 3413ARC → DRR in CAA27211. Ref.2
Sequence conflict3671Q → E in CAA27211. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P04773 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 611D58CE20FB16CF

FASTA37342,320
        10         20         30         40         50         60 
MATSASSHLN KGIKQMYMSL PQGEKVQAMY IWVDGTGEGL RCKTRTLDCE PKCVEELPEW 

        70         80         90        100        110        120 
NFDGSSTFQS ESSNSDMYLS PVAMFRDPFR KEPNKLVFCE VFKYNQKPAE TNLRHTCKRI 

       130        140        150        160        170        180 
MDMVSNQHPW FGMEQEYTLL GTDGHPFGWP SDGFPGPQGL YYCGVGADKA YRRDIMEAHY 

       190        200        210        220        230        240 
RACLYAGVKI TGTYAEVKHA QWEFQIGPCE GIRMGDHLWV ARFILHRVCK DFGVIATFDS 

       250        260        270        280        290        300 
KPIPGNWNGA GCHTNFSTKT MREENGLKHI KEAIEKLSKR HRYHIRAYDP KGGLDNARRL 

       310        320        330        340        350        360 
TGFHKTSNIN DFSAGVADRS ASIRIPRTVG QEKKGYFEAR CPSANCDPFA VTEAIVRTCL 

       370 
LNETGDQPFQ YKN 

« Hide

References

[1]"The cloning and nucleotide sequence of cDNA for an amplified glutamine synthetase gene from the Chinese hamster."
Hayward B.E., Hussain A., Wilson R.H., Lyons A., Woodcock V., McIntosh B., Harris T.J.R.
Nucleic Acids Res. 14:999-1008(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ovary.
[2]Tong Y., Wang H.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03495 mRNA. Translation: CAA27211.1.
AF150961 mRNA. Translation: AAG43362.1.
RefSeqNP_001233699.1. NM_001246770.1.

3D structure databases

ProteinModelPortalP04773.
SMRP04773. Positions 3-372.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100689337.
KEGGcge:100689337.

Organism-specific databases

CTD2752.

Phylogenomic databases

HOVERGENHBG005847.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_CRIGR
AccessionPrimary (citable) accession number: P04773
Secondary accession number(s): Q9EQP8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: November 13, 2013
This is version 86 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families