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P04773

- GLNA_CRIGR

UniProt

P04773 - GLNA_CRIGR

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Protein
Glutamine synthetase
Gene
GLUL
Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Biotin By similarity.
Magnesium or manganese By similarity.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate decarboxylase activity Source: UniProtKB-EC
  3. glutamate-ammonia ligase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Lyase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase (EC:6.3.1.2)
Short name:
GS
Alternative name(s):
Glutamate decarboxylase (EC:4.1.1.15)
Glutamate--ammonia ligase
Gene namesi
Name:GLUL
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Cytoplasm By similarity. Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 373372Glutamine synthetase
PRO_0000153138Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei104 – 1041Phosphotyrosine By similarity

Post-translational modificationi

Ubiquitinated by ZNRF1 By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Homooctamer and homotetramer. Interacts with PALMD By similarity.

Structurei

3D structure databases

ProteinModelPortaliP04773.
SMRiP04773. Positions 3-372.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG005847.
KOiK01915.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04773-1 [UniParc]FASTAAdd to Basket

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MATSASSHLN KGIKQMYMSL PQGEKVQAMY IWVDGTGEGL RCKTRTLDCE    50
PKCVEELPEW NFDGSSTFQS ESSNSDMYLS PVAMFRDPFR KEPNKLVFCE 100
VFKYNQKPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLL GTDGHPFGWP 150
SDGFPGPQGL YYCGVGADKA YRRDIMEAHY RACLYAGVKI TGTYAEVKHA 200
QWEFQIGPCE GIRMGDHLWV ARFILHRVCK DFGVIATFDS KPIPGNWNGA 250
GCHTNFSTKT MREENGLKHI KEAIEKLSKR HRYHIRAYDP KGGLDNARRL 300
TGFHKTSNIN DFSAGVADRS ASIRIPRTVG QEKKGYFEAR CPSANCDPFA 350
VTEAIVRTCL LNETGDQPFQ YKN 373
Length:373
Mass (Da):42,320
Last modified:January 23, 2007 - v4
Checksum:i611D58CE20FB16CF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121G → N in CAA27211. 1 Publication
Sequence conflicti18 – 192MS → LC in CAA27211. 1 Publication
Sequence conflicti72 – 721S → G in CAA27211. 1 Publication
Sequence conflicti91 – 922KE → RD in CAA27211. 1 Publication
Sequence conflicti106 – 1061Q → R in CAA27211. 1 Publication
Sequence conflicti116 – 1161T → S in CAA27211. 1 Publication
Sequence conflicti140 – 1401L → M in CAA27211. 1 Publication
Sequence conflicti152 – 1521D → N in CAA27211. 1 Publication
Sequence conflicti160 – 1601L → P in CAA27211. 1 Publication
Sequence conflicti172 – 1721R → G in CAA27211. 1 Publication
Sequence conflicti176 – 1761M → V in CAA27211. 1 Publication
Sequence conflicti194 – 1941Y → N in CAA27211. 1 Publication
Sequence conflicti198 – 1992KH → MP in CAA27211. 1 Publication
Sequence conflicti230 – 2301K → E in CAA27211. 1 Publication
Sequence conflicti240 – 2401S → P in CAA27211. 1 Publication
Sequence conflicti260 – 2601T → A in CAA27211. 1 Publication
Sequence conflicti271 – 2711K → E in CAA27211. 1 Publication
Sequence conflicti299 – 2991R → G in CAA27211. 1 Publication
Sequence conflicti305 – 3051K → E in CAA27211. 1 Publication
Sequence conflicti318 – 3181D → N in CAA27211. 1 Publication
Sequence conflicti339 – 3413ARC → DRR in CAA27211. 1 Publication
Sequence conflicti367 – 3671Q → E in CAA27211. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03495 mRNA. Translation: CAA27211.1.
AF150961 mRNA. Translation: AAG43362.1.
RefSeqiNP_001233699.1. NM_001246770.1.

Genome annotation databases

GeneIDi100689337.
KEGGicge:100689337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03495 mRNA. Translation: CAA27211.1 .
AF150961 mRNA. Translation: AAG43362.1 .
RefSeqi NP_001233699.1. NM_001246770.1.

3D structure databases

ProteinModelPortali P04773.
SMRi P04773. Positions 3-372.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100689337.
KEGGi cge:100689337.

Organism-specific databases

CTDi 2752.

Phylogenomic databases

HOVERGENi HBG005847.
KOi K01915.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The cloning and nucleotide sequence of cDNA for an amplified glutamine synthetase gene from the Chinese hamster."
    Hayward B.E., Hussain A., Wilson R.H., Lyons A., Woodcock V., McIntosh B., Harris T.J.R.
    Nucleic Acids Res. 14:999-1008(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.
  2. Tong Y., Wang H.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGLNA_CRIGR
AccessioniPrimary (citable) accession number: P04773
Secondary accession number(s): Q9EQP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 87 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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