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Protein

Glutamine synthetase

Gene

glnII

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109MagnesiumBy similarity1
Metal bindingi111MagnesiumBy similarity1
Binding sitei167ATPBy similarity1
Metal bindingi172MagnesiumBy similarity1
Metal bindingi179MagnesiumBy similarity1
Binding sitei278L-glutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase1 Publication (EC:6.3.1.2By similarity)
Short name:
GS1 Publication
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase II1 Publication
Short name:
GSII1 Publication
Gene namesi
Name:glnII1 Publication
Ordered Locus Names:blr4169
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532201 – 344Glutamine synthetaseAdd BLAST344

Interactioni

Subunit structurei

Homooctamer and homotetramer.By similarity

Protein-protein interaction databases

STRINGi224911.blr4169.

Structurei

3D structure databases

ProteinModelPortaliP04772.
SMRiP04772.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4107R5B. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000061500.
InParanoidiP04772.
KOiK01915.
OMAiDRRPNAN.
OrthoDBiPOG091H0CKA.
PhylomeDBiP04772.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P04772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKYKLEYIW LDGYTPTPNL RGKTQIKEFA SFPTLEQLPL WGFDGSSTQQ
60 70 80 90 100
AEGHSSDCVL KPVAVFPDAA RTNGVLVMCE VMMPDGKTPH ASNKRATILD
110 120 130 140 150
DAGAWFGFEQ EYFFYKDGRP LGFPTSGYPA PQGPYYTGVG FSNVGDVARK
160 170 180 190 200
IVEEHLDLCL AAGINHEGIN AEVAKGQWEF QIFGKGSKKA ADEMWMARYL
210 220 230 240 250
MLRLTEKYGI DIEFHCKPLG DTDWNGSGMH ANFSTEYMRT VGGKEYFEAL
260 270 280 290 300
MAAFDKNLMD HIAVYGPDND KRLTGKHETA PWNKFSYGVA DRGASIRVPH
310 320 330 340
SFVNNGYKGY LEDRRPNSQG DPYQIASQIL KTISSVPTEK KAVA
Length:344
Mass (Da):38,366
Last modified:February 28, 2003 - v2
Checksum:i5462522D03DC51B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37 – 38QL → HV in CAA27779 (Ref. 1) Curated2
Sequence conflicti326 – 344ASQIL…KKAVA → VRRS in CAA27779 (Ref. 1) CuratedAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04187 Genomic DNA. Translation: CAA27779.1.
BA000040 Genomic DNA. Translation: BAC49434.1.
PIRiA24155. AJZJQ2.
RefSeqiNP_770809.1. NC_004463.1.
WP_011086942.1. NZ_CP011360.1.

Genome annotation databases

EnsemblBacteriaiBAC49434; BAC49434; BAC49434.
GeneIDi1054220.
KEGGibja:blr4169.
PATRICi21191856. VBIBraJap65052_4184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04187 Genomic DNA. Translation: CAA27779.1.
BA000040 Genomic DNA. Translation: BAC49434.1.
PIRiA24155. AJZJQ2.
RefSeqiNP_770809.1. NC_004463.1.
WP_011086942.1. NZ_CP011360.1.

3D structure databases

ProteinModelPortaliP04772.
SMRiP04772.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr4169.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC49434; BAC49434; BAC49434.
GeneIDi1054220.
KEGGibja:blr4169.
PATRICi21191856. VBIBraJap65052_4184.

Phylogenomic databases

eggNOGiENOG4107R5B. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000061500.
InParanoidiP04772.
KOiK01915.
OMAiDRRPNAN.
OrthoDBiPOG091H0CKA.
PhylomeDBiP04772.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA2_BRADU
AccessioniPrimary (citable) accession number: P04772
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 28, 2003
Last modified: March 15, 2017
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.