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P04772

- GLNA2_BRADU

UniProt

P04772 - GLNA2_BRADU

Protein

Glutamine synthetase 2

Gene

glnII

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (28 Feb 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-4195-MONOMER.
    RETL1328306-WGS:GSTH-3150-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase 2 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase II
    Glutamine synthetase II
    Short name:
    GSII
    Gene namesi
    Name:glnII
    Ordered Locus Names:blr4169
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 344344Glutamine synthetase 2PRO_0000153220Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer.

    Protein-protein interaction databases

    STRINGi224911.blr4169.

    Structurei

    3D structure databases

    ProteinModelPortaliP04772.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000061500.
    KOiK01915.
    OrthoDBiEOG6K3ZXS.
    PhylomeDBiP04772.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04772-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKYKLEYIW LDGYTPTPNL RGKTQIKEFA SFPTLEQLPL WGFDGSSTQQ    50
    AEGHSSDCVL KPVAVFPDAA RTNGVLVMCE VMMPDGKTPH ASNKRATILD 100
    DAGAWFGFEQ EYFFYKDGRP LGFPTSGYPA PQGPYYTGVG FSNVGDVARK 150
    IVEEHLDLCL AAGINHEGIN AEVAKGQWEF QIFGKGSKKA ADEMWMARYL 200
    MLRLTEKYGI DIEFHCKPLG DTDWNGSGMH ANFSTEYMRT VGGKEYFEAL 250
    MAAFDKNLMD HIAVYGPDND KRLTGKHETA PWNKFSYGVA DRGASIRVPH 300
    SFVNNGYKGY LEDRRPNSQG DPYQIASQIL KTISSVPTEK KAVA 344
    Length:344
    Mass (Da):38,366
    Last modified:February 28, 2003 - v2
    Checksum:i5462522D03DC51B2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti37 – 382QL → HV in CAA27779. 1 PublicationCurated
    Sequence conflicti326 – 34419ASQIL…KKAVA → VRRS in CAA27779. 1 PublicationCuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04187 Genomic DNA. Translation: CAA27779.1.
    BA000040 Genomic DNA. Translation: BAC49434.1.
    PIRiA24155. AJZJQ2.
    RefSeqiNP_770809.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC49434; BAC49434; BAC49434.
    GeneIDi1054220.
    KEGGibja:blr4169.
    PATRICi21191856. VBIBraJap65052_4184.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04187 Genomic DNA. Translation: CAA27779.1 .
    BA000040 Genomic DNA. Translation: BAC49434.1 .
    PIRi A24155. AJZJQ2.
    RefSeqi NP_770809.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali P04772.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.blr4169.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC49434 ; BAC49434 ; BAC49434 .
    GeneIDi 1054220.
    KEGGi bja:blr4169.
    PATRICi 21191856. VBIBraJap65052_4184.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000061500.
    KOi K01915.
    OrthoDBi EOG6K3ZXS.
    PhylomeDBi P04772.

    Enzyme and pathway databases

    BioCyci BJAP224911:GJEJ-4195-MONOMER.
    RETL1328306-WGS:GSTH-3150-MONOMER.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Apparent eukaryotic origin of glutamine synthetase II from the bacterium Bradyrhizobium japonicum."
      Carlson T.A., Chelm B.K.
      Nature 322:568-570(1986)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiGLNA2_BRADU
    AccessioniPrimary (citable) accession number: P04772
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: February 28, 2003
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3