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Protein

Glutamine synthetase PR-2

Gene
N/A
Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Miscellaneous

There are at least four isozymes of this enzyme in P.vulgaris.
Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 4746.
SABIO-RKiP04771.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase PR-2 (EC:6.3.1.2)
Alternative name(s):
Gln isozyme alpha
Glutamate--ammonia ligase
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001531921 – 356Glutamine synthetase PR-2Add BLAST356

Expressioni

Tissue specificityi

Roots.

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP04771.
SMRiP04771.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

KOiK01915.
PhylomeDBiP04771.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P04771-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLSDLINL NLSESTEKII AEYIWVGGSG MDLRSKARTL PGPVDDPAKL
60 70 80 90 100
PKWNYDGSST DQAPGDDSEV ILYPQAIFKD PFRRGNNILV ICDVYTPAGE
110 120 130 140 150
PLPTNKRYDA AKIFSHPDVV AEVPWYGIEQ EYTLLQKDVN WPLGWPLGGY
160 170 180 190 200
PGPQGPYYCG VGADKAYGRD IVDAHYKACV YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSVGISA GDEVWAARYI LERITELAGA VVSFDPKPIP GDWNGAGAHS
260 270 280 290 300
NYSTKSMREE GGYEVIKKAI EKLGLRHKEH IAAYGKGNER RLTGRHETAD
310 320 330 340 350
INTFSWGVAN RGSSVRVGRD TEKQGKGYFE DRRPASNMDP YVVTSMIAET

TILWKP
Length:356
Mass (Da):39,205
Last modified:August 13, 1987 - v1
Checksum:i0001776952B2E0EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04002 mRNA. Translation: CAA27632.1.
PIRiB26308. AJFBQA.
RefSeqiXP_007152660.1. XM_007152598.1.

Genome annotation databases

GeneIDi18633095.
KEGGipvu:PHAVU_004G148300g.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLNA2_PHAVU
AccessioniPrimary (citable) accession number: P04771
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 12, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families