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P04770 (GLNA1_PHAVU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase PR-1

EC=6.3.1.2
Alternative name(s):
Gln isozyme beta
Glutamate--ammonia ligase
OrganismPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifier3885 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Cytoplasm.

Tissue specificity

Roots.

Miscellaneous

There are at least four isozymes of this enzyme in P.vulgaris.

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Glutamine synthetase PR-1
PRO_0000153191

Sequences

Sequence LengthMass (Da)Tools
P04770 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: E050631D3DBB8675

FASTA35639,118
        10         20         30         40         50         60 
MSLLSDLINL NLSDTTEKVI AEYIWIGGSG LDLRSKARTL PGPVKNPSEL PKWNYDGSST 

        70         80         90        100        110        120 
GQAPGQDSEV IIYPQAIFKD PFRRGNNILV ICDAYTPAGE PIPTNKRHNA AKIFSNPDVV 

       130        140        150        160        170        180 
AEEPWYGIEQ EYTLLQKEVN WPVGWPVGGF PGPQGPYYCG VGADKAFGRD IVDAHYKACV 

       190        200        210        220        230        240 
YAGINISGIN GEVMPGQWEF QVGPAVGISA GDELWVARYI LERITEVAGV VLSFDPKPIK 

       250        260        270        280        290        300 
GDWNGAGAHT NYSTKTMRND GGYEEIKSAI QKLGKRHKEH IAAYGEGNER RLTGRHETAD 

       310        320        330        340        350 
INTFLWGVAN RGASIRVGRD TEKAGKGYFE DRRPASNMDP YVVTSMIADT TILWKP 

« Hide

References

[1]"Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris."
Gebhardt C., Oliver J.E., Forde B.G., Saarelainen R., Miflin B.J.
EMBO J. 5:1429-1435(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04001 mRNA. Translation: CAA27631.1.
PIRAJFBQB. A26308.

3D structure databases

ProteinModelPortalP04770.
SMRP04770. Positions 4-355.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

ProMEXP04770.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA1_PHAVU
AccessionPrimary (citable) accession number: P04770
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families