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P04760 (ACHG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetylcholine receptor subunit gamma
Gene names
Name:Chrng
Synonyms:Acrg
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length519 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Subunit structure

Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.

Subcellular location

Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Tissue specificity

At least in myotubes of skeletal muscle.

Developmental stage

Both short and long isoforms are found in a 17 days old embryo, whereas the short isoform is the only isoform present in the newborn muscle.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Gamma/CHRNG sub-subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P04760-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P04760-2)

The sequence of this isoform differs from the canonical sequence as follows:
     117-168: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 519497Acetylcholine receptor subunit gamma
PRO_0000000335

Regions

Topological domain23 – 240218Extracellular Potential
Transmembrane241 – 26525Helical; Potential
Transmembrane274 – 29219Helical; Potential
Transmembrane308 – 32922Helical; Potential
Topological domain330 – 476147Cytoplasmic Potential
Transmembrane477 – 49721Helical; Potential

Amino acid modifications

Glycosylation521N-linked (GlcNAc...) Potential
Glycosylation1631N-linked (GlcNAc...) Potential
Disulfide bond150 ↔ 164 By similarity

Natural variations

Alternative sequence117 – 16852Missing in isoform Short.
VSP_000072

Experimental info

Sequence conflict2311V → G in AAB63431. Ref.2
Sequence conflict3461L → V in AAB63431. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 3F435303564C8048

FASTA51958,745
        10         20         30         40         50         60 
MQGGQRPHLL LLLLAVCLGA QSRNQEERLL ADLMRNYDPH LRPAERDSDV VNVSLKLTLT 

        70         80         90        100        110        120 
NLISLNEREE ALTTNVWIEM QWCDYRLRWD PKDYEGLWIL RVPSTMVWRP DIVLENNVDG 

       130        140        150        160        170        180 
VFEVALYCNV LVSPDGCIYW LPPAIFRSSC SISVTYFPFD WQNCSLIFQS QTYSTSEINL 

       190        200        210        220        230        240 
QLSQEDGQAI EWIFIDPEAF TENGEWAIRH RPAKMLLDSV APAEEAGHQK VVFYLLIQRK 

       250        260        270        280        290        300 
PLFYVINIIA PCVLISSVAI LIYFLPAKAG GQKCTVATNV LLAQTVFLFL VAKKVPETSQ 

       310        320        330        340        350        360 
AVPLISKYLT FLMVVTILIV VNSVVVLNVS LRSPHTHSMA RGVRKLFLRL LPQLLRMHVR 

       370        380        390        400        410        420 
PLAPAAVQDA RFRLQNGSSS GWPIMAREEG DLCLPRSELL FRQRQRNGLV QAVLEKLENG 

       430        440        450        460        470        480 
PEVRQSQEFC GSLKQASPAI QACVDACNLM ARARRQQSHF DSGNEEWLLV GRVLDRVCFL 

       490        500        510 
AMLSLFICGT AGIFLMAHYN QVPDLPFPGD PRPYLPLPD

« Hide

Isoform Short [UniParc].

Checksum: 03077383E5BA2E92
Show »

FASTA46752,866

References

[1]"Mouse muscle nicotinic acetylcholine receptor gamma subunit: cDNA sequence and gene expression."
Yu L., Lapolla R.J., Davidson N.
Nucleic Acids Res. 14:3539-3555(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Isolation and sequence of cDNA clones coding for the precursor to the gamma subunit of mouse muscle nicotinic acetylcholine receptor."
Boulter J., Evans K., Martin G., Mason P., Stengelin S., Goldman D.J., Heinemann S.F., Patrick J.
J. Neurosci. Res. 16:37-49(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Transcriptional regulation of nicotinic acetylcholine receptor genes: identification of control elements of a gamma-subunit gene."
Gardner P.D., Heinemann S.F., Patrick J.
Brain Res. 427:69-76(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-57.
Tissue: Muscle.
[4]"Stepwise activation of the mouse acetylcholine receptor delta- and gamma-subunit genes in clonal cell lines."
Crowder C.M., Merlie J.P.
Mol. Cell. Biol. 8:5257-5267(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
Strain: BALB/c.
[5]"Two forms of acetylcholine receptor gamma subunit in mouse muscle."
Mileo A.M., Monaco L., Palma E., Grassi F., Miledi R., Eusebi F.
Proc. Natl. Acad. Sci. U.S.A. 92:2686-2690(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-170 (ISOFORMS LONG AND SHORT).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X03818 mRNA. Translation: CAA27442.1.
X03819 mRNA. Translation: CAA27443.1.
M30514 mRNA. Translation: AAB63431.1. Sequence problems.
M27455 Genomic DNA. Translation: AAA70247.1.
M22381 Genomic DNA. Translation: AAA37152.1.
S77465 mRNA. Translation: AAB33997.2.
IPIIPI00118671.
IPI00224935.
PIRI56566.
UniGeneMm.2810.

3D structure databases

ProteinModelPortalP04760.
SMRP04760. Positions 24-338.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000027470.

PTM databases

PhosphoSiteP04760.

Proteomic databases

PRIDEP04760.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCuc007bwh.1. mouse.

Organism-specific databases

MGIMGI:87895. Chrng.

Phylogenomic databases

eggNOGNOG291810.
HOGENOMHOG000006757.
HOVERGENHBG003756.
InParanoidP04760.
OrthoDBEOG4R7V9N.

Gene expression databases

GenevestigatorP04760.
GermOnlineENSMUSG00000026253. Mus musculus.

Family and domain databases

Gene3D2.70.170.10. 1 hit.
InterProIPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF90112. Neu_channel_TM. 1 hit.
SSF63712. Neur_chan_LBD. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP04760.
ChEMBLCHEMBL4579.
SOURCESearch...

Entry information

Entry nameACHG_MOUSE
AccessionPrimary (citable) accession number: P04760
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: May 1, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents