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Protein

Acetylcholine receptor subunit gamma

Gene

Chrng

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  • acetylcholine-activated cation-selective channel activity Source: MGI

GO - Biological processi

  • cation transmembrane transport Source: GOC
  • regulation of membrane potential Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholine receptor subunit gamma
Gene namesi
Name:Chrng
Synonyms:Acrg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:87895. Chrng.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 240218ExtracellularSequence analysisAdd
BLAST
Transmembranei241 – 26525HelicalSequence analysisAdd
BLAST
Transmembranei274 – 29219HelicalSequence analysisAdd
BLAST
Transmembranei308 – 32922HelicalSequence analysisAdd
BLAST
Topological domaini330 – 476147CytoplasmicSequence analysisAdd
BLAST
Transmembranei477 – 49721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: MGI
  • cell junction Source: UniProtKB-KW
  • plasma membrane Source: MGI
  • postsynaptic membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3038460.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 519497Acetylcholine receptor subunit gammaPRO_0000000335Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi52 – 521N-linked (GlcNAc...)Sequence analysis
Disulfide bondi150 ↔ 164By similarity
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP04760.
PRIDEiP04760.

PTM databases

PhosphoSiteiP04760.

Expressioni

Tissue specificityi

At least in myotubes of skeletal muscle.

Developmental stagei

Both short and long isoforms are found in a 17 days old embryo, whereas the short isoform is the only isoform present in the newborn muscle.

Interactioni

Subunit structurei

Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027470.

Structurei

3D structure databases

ProteinModelPortaliP04760.
SMRiP04760. Positions 24-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP04760.
PhylomeDBiP04760.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P04760-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQGGQRPHLL LLLLAVCLGA QSRNQEERLL ADLMRNYDPH LRPAERDSDV
60 70 80 90 100
VNVSLKLTLT NLISLNEREE ALTTNVWIEM QWCDYRLRWD PKDYEGLWIL
110 120 130 140 150
RVPSTMVWRP DIVLENNVDG VFEVALYCNV LVSPDGCIYW LPPAIFRSSC
160 170 180 190 200
SISVTYFPFD WQNCSLIFQS QTYSTSEINL QLSQEDGQAI EWIFIDPEAF
210 220 230 240 250
TENGEWAIRH RPAKMLLDSV APAEEAGHQK VVFYLLIQRK PLFYVINIIA
260 270 280 290 300
PCVLISSVAI LIYFLPAKAG GQKCTVATNV LLAQTVFLFL VAKKVPETSQ
310 320 330 340 350
AVPLISKYLT FLMVVTILIV VNSVVVLNVS LRSPHTHSMA RGVRKLFLRL
360 370 380 390 400
LPQLLRMHVR PLAPAAVQDA RFRLQNGSSS GWPIMAREEG DLCLPRSELL
410 420 430 440 450
FRQRQRNGLV QAVLEKLENG PEVRQSQEFC GSLKQASPAI QACVDACNLM
460 470 480 490 500
ARARRQQSHF DSGNEEWLLV GRVLDRVCFL AMLSLFICGT AGIFLMAHYN
510
QVPDLPFPGD PRPYLPLPD
Length:519
Mass (Da):58,745
Last modified:August 13, 1987 - v1
Checksum:i3F435303564C8048
GO
Isoform Short (identifier: P04760-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-168: Missing.

Show »
Length:467
Mass (Da):52,866
Checksum:i03077383E5BA2E92
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti231 – 2311V → G in AAB63431 (PubMed:3755765).Curated
Sequence conflicti346 – 3461L → V in AAB63431 (PubMed:3755765).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei117 – 16852Missing in isoform Short. 1 PublicationVSP_000072Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03818 mRNA. Translation: CAA27442.1.
X03819 mRNA. Translation: CAA27443.1.
M30514 mRNA. Translation: AAB63431.1. Sequence problems.
M27455 Genomic DNA. Translation: AAA70247.1.
M22381 Genomic DNA. Translation: AAA37152.1.
S77465 mRNA. Translation: AAB33997.2.
CCDSiCCDS35651.1. [P04760-1]
PIRiI56566.
UniGeneiMm.2810.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03818 mRNA. Translation: CAA27442.1.
X03819 mRNA. Translation: CAA27443.1.
M30514 mRNA. Translation: AAB63431.1. Sequence problems.
M27455 Genomic DNA. Translation: AAA70247.1.
M22381 Genomic DNA. Translation: AAA37152.1.
S77465 mRNA. Translation: AAB33997.2.
CCDSiCCDS35651.1. [P04760-1]
PIRiI56566.
UniGeneiMm.2810.

3D structure databases

ProteinModelPortaliP04760.
SMRiP04760. Positions 24-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027470.

Chemistry

ChEMBLiCHEMBL3038460.

PTM databases

PhosphoSiteiP04760.

Proteomic databases

PaxDbiP04760.
PRIDEiP04760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:87895. Chrng.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP04760.
PhylomeDBiP04760.

Miscellaneous databases

PROiP04760.
SOURCEiSearch...

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mouse muscle nicotinic acetylcholine receptor gamma subunit: cDNA sequence and gene expression."
    Yu L., Lapolla R.J., Davidson N.
    Nucleic Acids Res. 14:3539-3555(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and sequence of cDNA clones coding for the precursor to the gamma subunit of mouse muscle nicotinic acetylcholine receptor."
    Boulter J., Evans K., Martin G., Mason P., Stengelin S., Goldman D.J., Heinemann S.F., Patrick J.
    J. Neurosci. Res. 16:37-49(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Transcriptional regulation of nicotinic acetylcholine receptor genes: identification of control elements of a gamma-subunit gene."
    Gardner P.D., Heinemann S.F., Patrick J.
    Brain Res. 427:69-76(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-57.
    Tissue: Muscle.
  4. "Stepwise activation of the mouse acetylcholine receptor delta- and gamma-subunit genes in clonal cell lines."
    Crowder C.M., Merlie J.P.
    Mol. Cell. Biol. 8:5257-5267(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
    Strain: BALB/cJ.
  5. "Two forms of acetylcholine receptor gamma subunit in mouse muscle."
    Mileo A.M., Monaco L., Palma E., Grassi F., Miledi R., Eusebi F.
    Proc. Natl. Acad. Sci. U.S.A. 92:2686-2690(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-170 (ISOFORMS LONG AND SHORT).

Entry informationi

Entry nameiACHG_MOUSE
AccessioniPrimary (citable) accession number: P04760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: May 11, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.