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Protein

Neuronal acetylcholine receptor subunit alpha-3

Gene

Chrna3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  • acetylcholine-activated cation-selective channel activity Source: RGD
  • acetylcholine binding Source: RGD
  • acetylcholine receptor activity Source: RGD
  • drug binding Source: RGD
  • protein heterodimerization activity Source: RGD

GO - Biological processi

  • cation transmembrane transport Source: GOC
  • chemical synaptic transmission, postsynaptic Source: GOC
  • heart development Source: RGD
  • protein heterooligomerization Source: RGD
  • response to drug Source: RGD
  • response to inorganic substance Source: RGD
  • response to nicotine Source: RGD
  • signal transduction Source: GOC
  • synaptic transmission Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-3
Gene namesi
Name:Chrna3
Synonyms:Acra3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2345. Chrna3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 234209ExtracellularAdd
BLAST
Transmembranei235 – 25925HelicalAdd
BLAST
Transmembranei267 – 28519HelicalAdd
BLAST
Transmembranei301 – 32222HelicalAdd
BLAST
Topological domaini323 – 471149CytoplasmicAdd
BLAST
Transmembranei472 – 49120HelicalAdd
BLAST

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: RGD
  • cell junction Source: UniProtKB-KW
  • dendrite Source: RGD
  • membrane Source: RGD
  • neuronal cell body Source: RGD
  • postsynaptic density Source: RGD
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3137275.
GuidetoPHARMACOLOGYi464.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 499474Neuronal acetylcholine receptor subunit alpha-3PRO_0000000348Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi49 – 491N-linked (GlcNAc...)Sequence analysis
Disulfide bondi153 ↔ 167By similarity
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence analysis
Disulfide bondi217 ↔ 218Associated with receptor activationBy similarity
Modified residuei407 – 4071PhosphoserineCombined sources
Modified residuei410 – 4101PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP04757.
PRIDEiP04757.

PTM databases

iPTMnetiP04757.
PhosphoSiteiP04757.

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-3 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly (By similarity).By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP04757. 1 interaction.
STRINGi10116.ENSRNOP00000019307.

Chemistry

BindingDBiP04757.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OLFmodel-A/C28-235[»]
1OLJmodel-A/C28-235[»]
ProteinModelPortaliP04757.
SMRiP04757. Positions 235-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP04757.
PhylomeDBiP04757.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVVLLPPPL SMLMLVLMLL PAASASEAEH RLFQYLFEDY NEIIRPVANV
60 70 80 90 100
SHPVIIQFEV SMSQLVKVDE VNQIMETNLW LKQIWNDYKL KWKPSDYQGV
110 120 130 140 150
EFMRVPAEKI WKPDIVLYNN ADGDFQVDDK TKALLKYTGE VTWIPPAIFK
160 170 180 190 200
SSCKIDVTYF PFDYQNCTMK FGSWSYDKAK IDLVLIGSSM NLKDYWESGE
210 220 230 240 250
WAIIKAPGYK HEIKYNCCEE IYQDITYSLY IRRLPLFYTI NLIIPCLLIS
260 270 280 290 300
FLTVLVFYLP SDCGEKVTLC ISVLLSLTVF LLVITETIPS TSLVIPLIGE
310 320 330 340 350
YLLFTMIFVT LSIVITVFVL NVHYRTPTTH TMPTWVKAVF LNLLPRVMFM
360 370 380 390 400
TRPTSGEGDT PKTRTFYGAE LSNLNCFSRA DSKSCKEGYP CQDGTCGYCH
410 420 430 440 450
HRRVKISNFS ANLTRSSSSE SVNAVLSLSA LSPEIKEAIQ SVKYIAENMK
460 470 480 490
AQNVAKEIQD DWKYVAMVID RIFLWVFILV CILGTAGLFL QPLMARDDT
Length:499
Mass (Da):56,998
Last modified:August 13, 1987 - v1
Checksum:iD66C491E832B9C34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03440 mRNA. Translation: CAA27170.1.
L31621 mRNA. Translation: AAA41673.1.
U04961 Unassigned DNA. Translation: AAA18001.1.
PIRiA24572.
A53733.
UniGeneiRn.10996.

Genome annotation databases

UCSCiRGD:2345. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03440 mRNA. Translation: CAA27170.1.
L31621 mRNA. Translation: AAA41673.1.
U04961 Unassigned DNA. Translation: AAA18001.1.
PIRiA24572.
A53733.
UniGeneiRn.10996.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OLFmodel-A/C28-235[»]
1OLJmodel-A/C28-235[»]
ProteinModelPortaliP04757.
SMRiP04757. Positions 235-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04757. 1 interaction.
STRINGi10116.ENSRNOP00000019307.

Chemistry

BindingDBiP04757.
ChEMBLiCHEMBL3137275.
GuidetoPHARMACOLOGYi464.

PTM databases

iPTMnetiP04757.
PhosphoSiteiP04757.

Proteomic databases

PaxDbiP04757.
PRIDEiP04757.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2345. rat.

Organism-specific databases

RGDi2345. Chrna3.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP04757.
PhylomeDBiP04757.

Miscellaneous databases

PROiP04757.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor alpha-subunit."
    Boulter J., Evans K., Goldman D.J., Martin G., Treco D., Heinemann S.F., Patrick J.
    Nature 319:368-374(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Functional expression of two neuronal nicotinic acetylcholine receptors from cDNA clones identifies a gene family."
    Boulter J., Connolly J.G., Deneris E.S., Goldman D.J., Heinemann S.F., Patrick J.
    Proc. Natl. Acad. Sci. U.S.A. 84:7763-7767(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Characterization of an acetylcholine receptor alpha 3 gene promoter and its activation by the POU domain factor SCIP/Tst-1."
    Yang X., McDonough J., Fyodorov D., Morris M., Wang F., Deneris E.S.
    J. Biol. Chem. 269:10252-10264(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    Tissue: Liver.
  4. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407 AND SER-410, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiACHA3_RAT
AccessioniPrimary (citable) accession number: P04757
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: May 11, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.