Neuronal acetylcholine receptor subunit alpha-3 precursor

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P04757 (ACHA3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Neuronal acetylcholine receptor subunit alpha-3

Gene names

Name:	Chrna3
Synonyms:	Acra3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Subunit structure	Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-3 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly By similarity.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heart development Inferred from expression pattern PubMed 15549397. Source: RGD protein heterooligomerization Inferred from direct assay PubMed 16129735. Source: RGD response to drug Inferred from direct assay PubMed 15896488. Source: RGD response to inorganic substance Inferred from expression pattern PubMed 15212813. Source: RGD response to nicotine Inferred from expression pattern PubMed 17105949. Source: RGD
Cellular_component	acetylcholine-gated channel complex Inferred from direct assay PubMed 16129735. Source: RGD cell junction Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from direct assay PubMed 11297818. Source: RGD postsynaptic density Inferred from direct assay PubMed 14989600. Source: RGD postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetylcholine binding Inferred from direct assay PubMed 11297818 PubMed 15016836. Source: RGD acetylcholine receptor activity Inferred from direct assay Ref.2. Source: RGD acetylcholine-activated cation-selective channel activity Inferred from mutant phenotype PubMed 11297818. Source: RGD drug binding Inferred from direct assay PubMed 15016836 PubMed 16129735. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

Potential

Chain

26 – 499

474

Neuronal acetylcholine receptor subunit alpha-3

PRO_0000000348

Regions

Topological domain

26 – 234

209

Extracellular

Transmembrane

235 – 259

Helical

Transmembrane

267 – 285

Helical

Transmembrane

301 – 322

Helical

Topological domain

323 – 471

149

Cytoplasmic

Transmembrane

472 – 491

Helical

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

166

N-linked (GlcNAc...) Potential

Disulfide bond

153 ↔ 167

By similarity

Disulfide bond

217 ↔ 218

Associated with receptor activation By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P04757 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: D66C491E832B9C34
Show »

FASTA

499

56,998

        10         20         30         40         50         60 
MGVVLLPPPL SMLMLVLMLL PAASASEAEH RLFQYLFEDY NEIIRPVANV SHPVIIQFEV 

        70         80         90        100        110        120 
SMSQLVKVDE VNQIMETNLW LKQIWNDYKL KWKPSDYQGV EFMRVPAEKI WKPDIVLYNN 

       130        140        150        160        170        180 
ADGDFQVDDK TKALLKYTGE VTWIPPAIFK SSCKIDVTYF PFDYQNCTMK FGSWSYDKAK 

       190        200        210        220        230        240 
IDLVLIGSSM NLKDYWESGE WAIIKAPGYK HEIKYNCCEE IYQDITYSLY IRRLPLFYTI 

       250        260        270        280        290        300 
NLIIPCLLIS FLTVLVFYLP SDCGEKVTLC ISVLLSLTVF LLVITETIPS TSLVIPLIGE 

       310        320        330        340        350        360 
YLLFTMIFVT LSIVITVFVL NVHYRTPTTH TMPTWVKAVF LNLLPRVMFM TRPTSGEGDT 

       370        380        390        400        410        420 
PKTRTFYGAE LSNLNCFSRA DSKSCKEGYP CQDGTCGYCH HRRVKISNFS ANLTRSSSSE 

       430        440        450        460        470        480 
SVNAVLSLSA LSPEIKEAIQ SVKYIAENMK AQNVAKEIQD DWKYVAMVID RIFLWVFILV 

       490 
CILGTAGLFL QPLMARDDT

« Hide

References

[1]	"Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor alpha-subunit." Boulter J., Evans K., Goldman D.J., Martin G., Treco D., Heinemann S.F., Patrick J. Nature 319:368-374(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Functional expression of two neuronal nicotinic acetylcholine receptors from cDNA clones identifies a gene family." Boulter J., Connolly J.G., Deneris E.S., Goldman D.J., Heinemann S.F., Patrick J. Proc. Natl. Acad. Sci. U.S.A. 84:7763-7767(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Characterization of an acetylcholine receptor alpha 3 gene promoter and its activation by the POU domain factor SCIP/Tst-1." Yang X., McDonough J., Fyodorov D., Morris M., Wang F., Deneris E.S. J. Biol. Chem. 269:10252-10264(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03440 mRNA. Translation: CAA27170.1.
L31621 mRNA. Translation: AAA41673.1.
U04961 Unassigned DNA. Translation: AAA18001.1.

IPI

IPI00198781.

PIR

A24572.
A53733.

UniGene

Rn.10996.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OLF	model	-	A/C	28-235	[»]
1OLJ	model	-	A/C	28-235	[»]

ProteinModelPortal

P04757.

SMR

P04757. Positions 235-326.

ModBase

Search...

Protein-protein interaction databases

STRING

10116.ENSRNOP00000019307.

PTM databases

PhosphoSite

P04757.

Proteomic databases

PRIDE

P04757.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

UCSC

RGD:2345. rat.

Organism-specific databases

RGD

2345. Chrna3.

Phylogenomic databases

eggNOG

NOG290206.

HOGENOM

HOG000006756.

HOVERGEN

HBG003756.

InParanoid

P04757.

OrthoDB

EOG45B1FG.

Gene expression databases

ArrayExpress

P04757.

Genevestigator

P04757.

GermOnline

ENSRNOG00000013829. Rattus norvegicus.

Family and domain databases

Gene3D

1.20.120.370. 2 hits.
2.70.170.10. 1 hit.

InterPro

IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]

PANTHER

PTHR18945. PTHR18945. 1 hit.

Pfam

PF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]

PRINTS

PR00254. NICOTINICR.
PR00252. NRIONCHANNEL.

SUPFAM

SSF90112. Neu_channel_TM. 1 hit.
SSF63712. Neur_chan_LBD. 1 hit.

TIGRFAMs

TIGR00860. LIC. 1 hit.

PROSITE

PS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P04757.

ChEMBL

CHEMBL3818.

Entry information

Entry name

ACHA3_RAT

Accession

Primary (citable) accession number: P04757

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	May 29, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents