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Protein

Neuronal acetylcholine receptor subunit alpha-3

Gene

Chrna3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  • acetylcholine binding Source: RGD
  • acetylcholine-gated cation-selective channel activity Source: RGD
  • acetylcholine receptor activity Source: RGD
  • drug binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • serotonin-gated cation-selective channel activity Source: GO_Central

GO - Biological processi

  • acetylcholine receptor signaling pathway Source: ARUK-UCL
  • heart development Source: RGD
  • protein heterooligomerization Source: RGD
  • response to acetylcholine Source: ARUK-UCL
  • response to drug Source: RGD
  • response to inorganic substance Source: RGD
  • response to nicotine Source: RGD

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-3
Gene namesi
Name:Chrna3
Synonyms:Acra3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2345 Chrna3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 234ExtracellularAdd BLAST209
Transmembranei235 – 259HelicalAdd BLAST25
Transmembranei267 – 285HelicalAdd BLAST19
Transmembranei301 – 322HelicalAdd BLAST22
Topological domaini323 – 471CytoplasmicAdd BLAST149
Transmembranei472 – 491HelicalAdd BLAST20

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3818
GuidetoPHARMACOLOGYi464

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000034826 – 499Neuronal acetylcholine receptor subunit alpha-3Add BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi49N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi153 ↔ 167By similarity
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi217 ↔ 218Associated with receptor activationBy similarity
Modified residuei407PhosphoserineCombined sources1
Modified residuei410PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP04757
PRIDEiP04757

PTM databases

iPTMnetiP04757
PhosphoSitePlusiP04757

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-3 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly. Interacts with LYPD6. The heteropentamer alpha-3-beta-2 interacts with alpha-conotoxins ImI, ImII, PnIA, GID and MII (PubMed:15609996, PubMed:15929983).By similarity2 Publications

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP04757, 1 interactor
STRINGi10116.ENSRNOP00000019307

Chemistry databases

BindingDBiP04757

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OLFmodel-A/C28-235[»]
1OLJmodel-A/C28-235[»]
ProteinModelPortaliP04757
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiP04757
PhylomeDBiP04757

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04757-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVVLLPPPL SMLMLVLMLL PAASASEAEH RLFQYLFEDY NEIIRPVANV
60 70 80 90 100
SHPVIIQFEV SMSQLVKVDE VNQIMETNLW LKQIWNDYKL KWKPSDYQGV
110 120 130 140 150
EFMRVPAEKI WKPDIVLYNN ADGDFQVDDK TKALLKYTGE VTWIPPAIFK
160 170 180 190 200
SSCKIDVTYF PFDYQNCTMK FGSWSYDKAK IDLVLIGSSM NLKDYWESGE
210 220 230 240 250
WAIIKAPGYK HEIKYNCCEE IYQDITYSLY IRRLPLFYTI NLIIPCLLIS
260 270 280 290 300
FLTVLVFYLP SDCGEKVTLC ISVLLSLTVF LLVITETIPS TSLVIPLIGE
310 320 330 340 350
YLLFTMIFVT LSIVITVFVL NVHYRTPTTH TMPTWVKAVF LNLLPRVMFM
360 370 380 390 400
TRPTSGEGDT PKTRTFYGAE LSNLNCFSRA DSKSCKEGYP CQDGTCGYCH
410 420 430 440 450
HRRVKISNFS ANLTRSSSSE SVNAVLSLSA LSPEIKEAIQ SVKYIAENMK
460 470 480 490
AQNVAKEIQD DWKYVAMVID RIFLWVFILV CILGTAGLFL QPLMARDDT
Length:499
Mass (Da):56,998
Last modified:August 13, 1987 - v1
Checksum:iD66C491E832B9C34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03440 mRNA Translation: CAA27170.1
L31621 mRNA Translation: AAA41673.1
U04961 Unassigned DNA Translation: AAA18001.1
PIRiA24572
A53733
UniGeneiRn.10996

Genome annotation databases

UCSCiRGD:2345 rat

Similar proteinsi

Entry informationi

Entry nameiACHA3_RAT
AccessioniPrimary (citable) accession number: P04757
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: May 23, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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