Reviewed, UniProtKB/Swiss-Prot P04749 (AMY5_HORVU)

Names and origin

Protein names

Recommended name:
    Alpha-amylase type B isozyme
    EC=3.2.1.1
Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Clone 168

Gene names

Name:

AMY1.5

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	135 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.
Cofactor	Binds 3 calcium ions per subunit By similarity.
Subunit structure	Monomer By similarity.
Developmental stage	Production of alpha-amylase is hormonally regulated. Germinating embryos produce the hormone gibberellic acid, which within 10 hours stimulates the aleurone cells covering the endosperm of the seed to produce alpha-amylase. The enzyme then degrades the starch within the endosperm for use by the developing plant embryo.
Miscellaneous	There are at least 4 types of alpha-amylase in barley. Type B isozyme mRNA is undetectable in unstimulated cells and increases a hundred-fold after stimulation with gibberellic acid.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Germination
Ligand	Calcium
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 135	›135	Alpha-amylase type B isozyme		PRO_0000054293
Sites
	Active site	21	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P04749-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 947F71F5E13DD2A6	FASTA	135	15,140
	10 20 30 40 50 60 KAPGMIGWWP AKAVTFVNNH DTGSTQHMWP FPSDRVMQGY AYILTHQGTP CIFYDHFFDW 70 80 90 100 110 120 GPKEEIDRLV SVSTRHGIHS ESKLQIIEAD ADLCLAEIDG KVIVKLGPRY DVGNLIPGGF 130 EVAAHGNDYA VWEKI

References

[1]	"Expression and regulation of alpha-amylase gene family in barley aleurones." Huang J.-K., Swegle M., Dandekar A.M., Muthukrishnan S. J. Mol. Appl. Genet. 2:579-588(1984) [PubMed: 6335720] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02635 mRNA. Translation: AAA32931.1.
PIR	C21826.
3D structure databases
SMR	P04749. Positions 1-135.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Organism-specific databases
Gramene	P04749.
Enzyme and pathway databases
BRENDA	3.2.1.1. 283.
Gene expression databases
Genevestigator	P04749.
Family and domain databases
InterPro	IPR012850. A-amylase_bs_C. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF07821. Alpha-amyl_C2. 1 hit. [Graphical view]
SMART	SM00810. Alpha-amyl_C2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMY5_HORVU

Accession

Primary (citable) accession number: P04749

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	January 19, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)