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Reviewed, UniProtKB/Swiss-Prot P04749 (AMY5_HORVU)

Last modified January 19, 2010. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Alpha-amylase type B isozyme
    EC=3.2.1.1
Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Clone 168
Gene names
Name: AMY1.5
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Protein attributes

Sequence length135 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.

Cofactor

Binds 3 calcium ions per subunit By similarity.

Subunit structure

Monomer By similarity.

Developmental stage

Production of alpha-amylase is hormonally regulated. Germinating embryos produce the hormone gibberellic acid, which within 10 hours stimulates the aleurone cells covering the endosperm of the seed to produce alpha-amylase. The enzyme then degrades the starch within the endosperm for use by the developing plant embryo.

Miscellaneous

There are at least 4 types of alpha-amylase in barley.

Type B isozyme mRNA is undetectable in unstimulated cells and increases a hundred-fold after stimulation with gibberellic acid.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Germination
   LigandCalcium
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalpha-amylase activity

Inferred from electronic annotation. Source: EC

calcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 135›135Alpha-amylase type B isozyme
PRO_0000054293

Sites

Active site211 By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P04749-1 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 947F71F5E13DD2A6

FASTA13515,140
        10         20         30         40         50         60 
KAPGMIGWWP AKAVTFVNNH DTGSTQHMWP FPSDRVMQGY AYILTHQGTP CIFYDHFFDW 

        70         80         90        100        110        120 
GPKEEIDRLV SVSTRHGIHS ESKLQIIEAD ADLCLAEIDG KVIVKLGPRY DVGNLIPGGF 

       130 
EVAAHGNDYA VWEKI 

« Hide

References

[1]"Expression and regulation of alpha-amylase gene family in barley aleurones."
Huang J.-K., Swegle M., Dandekar A.M., Muthukrishnan S.
J. Mol. Appl. Genet. 2:579-588(1984) [PubMed: 6335720] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02635 mRNA. Translation: AAA32931.1.
PIRC21826.

3D structure databases

SMRP04749. Positions 1-135.
ModBaseSearch...

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Organism-specific databases

GrameneP04749.

Enzyme and pathway databases

BRENDA3.2.1.1. 283.

Gene expression databases

GenevestigatorP04749.

Family and domain databases

InterProIPR012850. A-amylase_bs_C.
IPR017853. Glyco_hydro_catalytic_core.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF07821. Alpha-amyl_C2. 1 hit.
[Graphical view]
SMARTSM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMY5_HORVU
AccessionPrimary (citable) accession number: P04749
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: January 19, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents