P04748 (AMY4_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase type B isozyme
EC=3.2.1.1

Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Clone 103

Gene names

Name:

AMY1.4

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	153 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Cofactor	Binds 3 calcium ions per subunit By similarity.
Subunit structure	Monomer By similarity.
Developmental stage	Production of alpha-amylase is hormonally regulated. Germinating embryos produce the hormone gibberellic acid, which within 10 hours stimulates the aleurone cells covering the endosperm of the seed to produce alpha-amylase. The enzyme then degrades the starch within the endosperm for use by the developing plant embryo.
Miscellaneous	There are at least 4 types of alpha-amylase in barley. Type B isozyme mRNA is undetectable in unstimulated cells and increases a hundred-fold after stimulation with gibberellic acid.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Germination
Ligand	Calcium
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 153	›153	Alpha-amylase type B isozyme		PRO_0000054292
Sites
	Site	39	1	Transition state stabilizer By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P04748 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: BE5233AE1D265F72	FASTA	153	17,276
	10 20 30 40 50 60 ILNVAVEGAL WRLRGTDGKA PSMIGWWPAK AVTFVDNHDT GSTQHMWPFP SDRVMQGYAY 70 80 90 100 110 120 ILTHPRTPCI FYDHFFDWGP KEEIDRLVSV RTRHGIHNES KLQIIEADAD LYLAEIDGKV 130 140 150 IVKLGPRYDV GNLIPGGFEG AAHGNDYAVW EKI

References

[1]	"Expression and regulation of alpha-amylase gene family in barley aleurones." Huang J.-K., Swegle M., Dandekar A.M., Muthukrishnan S. J. Mol. Appl. Genet. 2:579-588(1984) [PubMed: 6335720] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M10056 mRNA. Translation: AAA32930.1.
PIR	A21826.
3D structure databases
ProteinModelPortal	P04748.
SMR	P04748. Positions 1-153.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P04748.
Gene expression databases
Genevestigator	P04748.
Family and domain databases
InterPro	IPR012850. A-amylase_bs_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF07821. Alpha-amyl_C2. 1 hit. [Graphical view]
SMART	SM00810. Alpha-amyl_C2. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMY4_HORVU

Accession

Primary (citable) accession number: P04748

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program