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Protein

Alpha-amylase 1

Gene

AMY1A

more
Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.1 Publication
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115CalciumCombined sources2 Publications1
Metal bindingi173Calcium; via carbonyl oxygenCombined sources2 Publications1
Metal bindingi182CalciumCombined sources2 Publications1
Binding sitei210ChlorideCombined sources2 Publications1
Active sitei212Nucleophile1
Metal bindingi216Calcium; via carbonyl oxygenCombined sources2 Publications1
Active sitei248Proton donor1
Binding sitei313ChlorideCombined sources2 Publications1
Sitei315Transition state stabilizerBy similarity1
Binding sitei352ChlorideCombined sources2 Publications1

GO - Molecular functioni

  • alpha-amylase activity Source: ProtInc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • digestion Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131240-MONOMER.
ZFISH:HS10839-MONOMER.
BRENDAi3.2.1.1. 2681.
ReactomeiR-HSA-189085. Digestion of dietary carbohydrate.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 1 (EC:3.2.1.11 Publication)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase 1
Salivary alpha-amylase
Gene namesi
Name:AMY1A
Synonyms:AMY1
AND
Name:AMY1B
Synonyms:AMY1
AND
Name:AMY1C
Synonyms:AMY1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:474. AMY1A.
HGNC:475. AMY1B.
HGNC:476. AMY1C.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi276.
277.
278.
OpenTargetsiENSG00000174876.
ENSG00000187733.
ENSG00000237763.
PharmGKBiPA24783.

Chemistry databases

ChEMBLiCHEMBL2478.

Polymorphism and mutation databases

BioMutaiAMY1A.
DMDMi1351933.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
ChainiPRO_000000140116 – 511Alpha-amylase 1Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi43 ↔ 101Combined sources2 Publications
Disulfide bondi85 ↔ 130Combined sources2 Publications
Disulfide bondi156 ↔ 175Combined sources2 Publications
Modified residuei365Deamidated asparagine; partial1 Publication1
Disulfide bondi393 ↔ 399Combined sources2 Publications
Modified residuei427Deamidated asparagine; partial; alternate1 Publication1
Glycosylationi427N-linked (GlcNAc...)2 Publications1
Disulfide bondi465 ↔ 477Combined sources2 Publications
Modified residuei474Deamidated asparagine; partial1 Publication1
Glycosylationi476N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP04745.
PaxDbiP04745.
PeptideAtlasiP04745.
PRIDEiP04745.

PTM databases

iPTMnetiP04745.
PhosphoSitePlusiP04745.
SwissPalmiP04745.
UniCarbKBiP04745.

Expressioni

Gene expression databases

BgeeiENSG00000174876.
CleanExiHS_AMY1A.
ExpressionAtlasiP04745. differential.
GenevisibleiP04745. HS.

Organism-specific databases

HPAiCAB004310.
HPA045394.
HPA045399.
HPA046980.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
MUC7Q8TAX72EBI-738586,EBI-738582

Protein-protein interaction databases

BioGridi106773. 5 interactors.
106775. 28 interactors.
IntActiP04745. 3 interactors.
STRINGi9606.ENSP00000359100.

Chemistry databases

BindingDBiP04745.

Structurei

Secondary structure

1511
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 31Combined sources5
Helixi36 – 45Combined sources10
Turni46 – 51Combined sources6
Beta strandi54 – 57Combined sources4
Turni67 – 70Combined sources4
Helixi73 – 77Combined sources5
Beta strandi78 – 80Combined sources3
Helixi91 – 103Combined sources13
Beta strandi107 – 112Combined sources6
Beta strandi115 – 119Combined sources5
Beta strandi124 – 126Combined sources3
Turni136 – 139Combined sources4
Turni142 – 145Combined sources4
Helixi148 – 150Combined sources3
Turni153 – 155Combined sources3
Beta strandi158 – 162Combined sources5
Helixi169 – 174Combined sources6
Helixi177 – 179Combined sources3
Beta strandi180 – 183Combined sources4
Helixi188 – 204Combined sources17
Beta strandi208 – 211Combined sources4
Helixi214 – 216Combined sources3
Helixi219 – 226Combined sources8
Turni234 – 236Combined sources3
Beta strandi244 – 247Combined sources4
Beta strandi253 – 256Combined sources4
Helixi259 – 262Combined sources4
Turni263 – 265Combined sources3
Beta strandi266 – 269Combined sources4
Helixi272 – 282Combined sources11
Helixi289 – 294Combined sources6
Helixi297 – 299Combined sources3
Turni304 – 306Combined sources3
Beta strandi307 – 309Combined sources3
Helixi316 – 318Combined sources3
Beta strandi321 – 323Combined sources3
Helixi324 – 326Combined sources3
Helixi330 – 332Combined sources3
Helixi333 – 345Combined sources13
Beta strandi348 – 355Combined sources8
Beta strandi363 – 368Combined sources6
Turni369 – 372Combined sources4
Beta strandi375 – 378Combined sources4
Helixi400 – 402Combined sources3
Helixi404 – 415Combined sources12
Turni416 – 418Combined sources3
Beta strandi421 – 426Combined sources6
Beta strandi428 – 436Combined sources9
Turni437 – 439Combined sources3
Beta strandi440 – 445Combined sources6
Beta strandi447 – 449Combined sources3
Beta strandi451 – 456Combined sources6
Beta strandi461 – 465Combined sources5
Turni467 – 469Combined sources3
Beta strandi476 – 479Combined sources4
Beta strandi481 – 484Combined sources4
Beta strandi488 – 494Combined sources7
Beta strandi498 – 500Combined sources3
Beta strandi502 – 506Combined sources5
Helixi507 – 509Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C8QX-ray2.30A16-511[»]
1JXJX-ray1.99A16-511[»]
1JXKX-ray1.90A16-511[»]
1MFUX-ray2.00A17-511[»]
1MFVX-ray2.00A17-511[»]
1NM9X-ray2.10A16-511[»]
1Q4NX-ray2.07X16-511[»]
1SMDX-ray1.60A17-511[»]
1XV8X-ray3.00A/B16-511[»]
1Z32X-ray1.60X16-511[»]
3BLKX-ray2.00A16-511[»]
3BLPX-ray1.60X16-511[»]
3DHPX-ray1.50A16-511[»]
ProteinModelPortaliP04745.
SMRiP04745.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04745.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
GeneTreeiENSGT00390000002882.
HOGENOMiHOG000253313.
HOVERGENiHBG000061.
InParanoidiP04745.
KOiK01176.
OMAiNWDTERD.
OrthoDBiEOG091G07V6.
PhylomeDBiP04745.
TreeFamiTF312850.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLFWLLFTI GFCWAQYSSN TQQGRTSIVH LFEWRWVDIA LECERYLAPK
60 70 80 90 100
GFGGVQVSPP NENVAIHNPF RPWWERYQPV SYKLCTRSGN EDEFRNMVTR
110 120 130 140 150
CNNVGVRIYV DAVINHMCGN AVSAGTSSTC GSYFNPGSRD FPAVPYSGWD
160 170 180 190 200
FNDGKCKTGS GDIENYNDAT QVRDCRLSGL LDLALGKDYV RSKIAEYMNH
210 220 230 240 250
LIDIGVAGFR IDASKHMWPG DIKAILDKLH NLNSNWFPEG SKPFIYQEVI
260 270 280 290 300
DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG
310 320 330 340 350
FMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF
360 370 380 390 400
TRVMSSYRWP RYFENGKDVN DWVGPPNDNG VTKEVTINPD TTCGNDWVCE
410 420 430 440 450
HRWRQIRNMV NFRNVVDGQP FTNWYDNGSN QVAFGRGNRG FIVFNNDDWT
460 470 480 490 500
FSLTLQTGLP AGTYCDVISG DKINGNCTGI KIYVSDDGKA HFSISNSAED
510
PFIAIHAESK L
Length:511
Mass (Da):57,768
Last modified:February 1, 1996 - v2
Checksum:i7710BCAC83EBE8B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti285N → T in AAA57345 (PubMed:2442579).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18786
, M18715, M18717, M18719, M18721, M18723, M18725, M18727, M18784 Genomic DNA. Translation: AAA52279.1.
AK292341 mRNA. Translation: BAF85030.1.
AL513482 Genomic DNA. Translation: CAI14928.1.
AL513482 Genomic DNA. Translation: CAI14932.1.
BC063129 mRNA. Translation: AAH63129.1.
BC069347 mRNA. Translation: AAH69347.1.
BC069463 mRNA. Translation: AAH69463.1.
BC092444 mRNA. Translation: AAH92444.1.
BC132985 mRNA. Translation: AAI32986.1.
BC132987 mRNA. Translation: AAI32988.1.
BC132995 mRNA. Translation: AAI32996.1.
BC132997 mRNA. Translation: AAI32998.1.
M18671 Genomic DNA. Translation: AAA58368.1.
M18674 Genomic DNA. Translation: AAA16183.2.
M19233, M17883 Genomic DNA. Translation: AAA57345.1. Different termination.
M17884 Genomic DNA. No translation available.
CCDSiCCDS30782.1.
CCDS30783.1.
CCDS30784.1.
PIRiA91543. ALHUS.
RefSeqiNP_001008219.1. NM_001008218.1.
NP_001008220.1. NM_001008219.1.
NP_001008222.1. NM_001008221.1.
NP_004029.2. NM_004038.3.
XP_011539564.1. XM_011541262.1.
XP_011539565.1. XM_011541263.1.
XP_016856547.1. XM_017001058.1.
UniGeneiHs.599274.
Hs.654437.
Hs.655232.
Hs.662103.

Genome annotation databases

EnsembliENST00000330330; ENSP00000330484; ENSG00000174876.
ENST00000370079; ENSP00000359096; ENSG00000187733.
ENST00000370080; ENSP00000359097; ENSG00000174876.
ENST00000370083; ENSP00000359100; ENSG00000237763.
GeneIDi276.
277.
278.
KEGGihsa:276.
hsa:277.
hsa:278.
UCSCiuc001duv.4. human.

Cross-referencesi

Web resourcesi

Wikipedia

Amylase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18786
, M18715, M18717, M18719, M18721, M18723, M18725, M18727, M18784 Genomic DNA. Translation: AAA52279.1.
AK292341 mRNA. Translation: BAF85030.1.
AL513482 Genomic DNA. Translation: CAI14928.1.
AL513482 Genomic DNA. Translation: CAI14932.1.
BC063129 mRNA. Translation: AAH63129.1.
BC069347 mRNA. Translation: AAH69347.1.
BC069463 mRNA. Translation: AAH69463.1.
BC092444 mRNA. Translation: AAH92444.1.
BC132985 mRNA. Translation: AAI32986.1.
BC132987 mRNA. Translation: AAI32988.1.
BC132995 mRNA. Translation: AAI32996.1.
BC132997 mRNA. Translation: AAI32998.1.
M18671 Genomic DNA. Translation: AAA58368.1.
M18674 Genomic DNA. Translation: AAA16183.2.
M19233, M17883 Genomic DNA. Translation: AAA57345.1. Different termination.
M17884 Genomic DNA. No translation available.
CCDSiCCDS30782.1.
CCDS30783.1.
CCDS30784.1.
PIRiA91543. ALHUS.
RefSeqiNP_001008219.1. NM_001008218.1.
NP_001008220.1. NM_001008219.1.
NP_001008222.1. NM_001008221.1.
NP_004029.2. NM_004038.3.
XP_011539564.1. XM_011541262.1.
XP_011539565.1. XM_011541263.1.
XP_016856547.1. XM_017001058.1.
UniGeneiHs.599274.
Hs.654437.
Hs.655232.
Hs.662103.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C8QX-ray2.30A16-511[»]
1JXJX-ray1.99A16-511[»]
1JXKX-ray1.90A16-511[»]
1MFUX-ray2.00A17-511[»]
1MFVX-ray2.00A17-511[»]
1NM9X-ray2.10A16-511[»]
1Q4NX-ray2.07X16-511[»]
1SMDX-ray1.60A17-511[»]
1XV8X-ray3.00A/B16-511[»]
1Z32X-ray1.60X16-511[»]
3BLKX-ray2.00A16-511[»]
3BLPX-ray1.60X16-511[»]
3DHPX-ray1.50A16-511[»]
ProteinModelPortaliP04745.
SMRiP04745.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106773. 5 interactors.
106775. 28 interactors.
IntActiP04745. 3 interactors.
STRINGi9606.ENSP00000359100.

Chemistry databases

BindingDBiP04745.
ChEMBLiCHEMBL2478.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

PTM databases

iPTMnetiP04745.
PhosphoSitePlusiP04745.
SwissPalmiP04745.
UniCarbKBiP04745.

Polymorphism and mutation databases

BioMutaiAMY1A.
DMDMi1351933.

Proteomic databases

MaxQBiP04745.
PaxDbiP04745.
PeptideAtlasiP04745.
PRIDEiP04745.

Protocols and materials databases

DNASUi277.
278.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000330330; ENSP00000330484; ENSG00000174876.
ENST00000370079; ENSP00000359096; ENSG00000187733.
ENST00000370080; ENSP00000359097; ENSG00000174876.
ENST00000370083; ENSP00000359100; ENSG00000237763.
GeneIDi276.
277.
278.
KEGGihsa:276.
hsa:277.
hsa:278.
UCSCiuc001duv.4. human.

Organism-specific databases

CTDi276.
277.
278.
DisGeNETi276.
277.
278.
GeneCardsiAMY1A.
AMY1B.
AMY1C.
HGNCiHGNC:474. AMY1A.
HGNC:475. AMY1B.
HGNC:476. AMY1C.
HPAiCAB004310.
HPA045394.
HPA045399.
HPA046980.
MIMi104700. gene.
104701. gene.
104702. gene.
neXtProtiNX_P04745.
OpenTargetsiENSG00000174876.
ENSG00000187733.
ENSG00000237763.
PharmGKBiPA24783.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
GeneTreeiENSGT00390000002882.
HOGENOMiHOG000253313.
HOVERGENiHBG000061.
InParanoidiP04745.
KOiK01176.
OMAiNWDTERD.
OrthoDBiEOG091G07V6.
PhylomeDBiP04745.
TreeFamiTF312850.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131240-MONOMER.
ZFISH:HS10839-MONOMER.
BRENDAi3.2.1.1. 2681.
ReactomeiR-HSA-189085. Digestion of dietary carbohydrate.

Miscellaneous databases

EvolutionaryTraceiP04745.
GeneWikiiAMY1A.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000174876.
CleanExiHS_AMY1A.
ExpressionAtlasiP04745. differential.
GenevisibleiP04745. HS.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY1_HUMAN
AccessioniPrimary (citable) accession number: P04745
Secondary accession number(s): A6NJS5
, A8K8H6, Q13763, Q5T083
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 192 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.