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Protein

Metallothionein-1F

Gene

MT1F

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi5 – 51Divalent metal cation; cluster B
Metal bindingi7 – 71Divalent metal cation; cluster B
Metal bindingi13 – 131Divalent metal cation; cluster B
Metal bindingi15 – 151Divalent metal cation; cluster B
Metal bindingi19 – 191Divalent metal cation; cluster B
Metal bindingi21 – 211Divalent metal cation; cluster B
Metal bindingi24 – 241Divalent metal cation; cluster B
Metal bindingi26 – 261Divalent metal cation; cluster B
Metal bindingi29 – 291Divalent metal cation; cluster B
Metal bindingi33 – 331Divalent metal cation; cluster A
Metal bindingi34 – 341Divalent metal cation; cluster A
Metal bindingi36 – 361Divalent metal cation; cluster A
Metal bindingi37 – 371Divalent metal cation; cluster A
Metal bindingi41 – 411Divalent metal cation; cluster A
Metal bindingi44 – 441Divalent metal cation; cluster A
Metal bindingi48 – 481Divalent metal cation; cluster A
Metal bindingi50 – 501Divalent metal cation; cluster A
Metal bindingi57 – 571Divalent metal cation; cluster A
Metal bindingi59 – 591Divalent metal cation; cluster A
Metal bindingi60 – 601Divalent metal cation; cluster A

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Cadmium, Copper, Metal-binding, Metal-thiolate cluster, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-5661231. Metallothioneins bind metals.

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-1F
Short name:
MT-1F
Alternative name(s):
Metallothionein-IF
Short name:
MT-IF
Gene namesi
Name:MT1F
ORF Names:PRO0376
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:7398. MT1F.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31203.

Polymorphism and mutation databases

BioMutaiMT1F.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6161Metallothionein-1FPRO_0000197237Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei58 – 581PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP04733.
MaxQBiP04733.
PaxDbiP04733.
PRIDEiP04733.

PTM databases

iPTMnetiP04733.
PhosphoSiteiP04733.

Expressioni

Gene expression databases

BgeeiP04733.
CleanExiHS_MT1F.
ExpressionAtlasiP04733. baseline and differential.
GenevisibleiP04733. HS.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
NOP2P460873EBI-10209483,EBI-356811

Protein-protein interaction databases

BioGridi110600. 2 interactions.
IntActiP04733. 1 interaction.
STRINGi9606.ENSP00000334872.

Structurei

3D structure databases

ProteinModelPortaliP04733.
SMRiP04733. Positions 1-60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2929BetaAdd
BLAST
Regioni30 – 6132AlphaAdd
BLAST

Domaini

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IUWR. Eukaryota.
ENOG4110PT3. LUCA.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000043048.
InParanoidiP04733.
KOiK14739.
OMAiKECKCIS.
OrthoDBiEOG7PZS1X.
PhylomeDBiP04733.
TreeFamiTF336054.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR003019. Metalthion.
IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04733-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPNCSCAAG VSCTCAGSCK CKECKCTSCK KSCCSCCPVG CSKCAQGCVC
60
KGASEKCSCC D
Length:61
Mass (Da):6,086
Last modified:August 13, 1987 - v1
Checksum:i7C7A61E180D8D1C8
GO

Sequence cautioni

The sequence AAF23355.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10943 Genomic DNA. Translation: AAA59588.1.
M13003 Genomic DNA. Translation: AAA36213.1.
AF078844 mRNA. Translation: AAF23355.1. Different initiation.
BC029453 mRNA. Translation: AAH29453.1.
CCDSiCCDS32456.1.
PIRiB22634. SMHU1F.
RefSeqiNP_001288201.1. NM_001301272.1.
NP_005940.1. NM_005949.3.
UniGeneiHs.513626.

Genome annotation databases

EnsembliENST00000334350; ENSP00000334872; ENSG00000198417.
GeneIDi4494.
KEGGihsa:4494.
UCSCiuc002ejt.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10943 Genomic DNA. Translation: AAA59588.1.
M13003 Genomic DNA. Translation: AAA36213.1.
AF078844 mRNA. Translation: AAF23355.1. Different initiation.
BC029453 mRNA. Translation: AAH29453.1.
CCDSiCCDS32456.1.
PIRiB22634. SMHU1F.
RefSeqiNP_001288201.1. NM_001301272.1.
NP_005940.1. NM_005949.3.
UniGeneiHs.513626.

3D structure databases

ProteinModelPortaliP04733.
SMRiP04733. Positions 1-60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110600. 2 interactions.
IntActiP04733. 1 interaction.
STRINGi9606.ENSP00000334872.

PTM databases

iPTMnetiP04733.
PhosphoSiteiP04733.

Polymorphism and mutation databases

BioMutaiMT1F.

Proteomic databases

EPDiP04733.
MaxQBiP04733.
PaxDbiP04733.
PRIDEiP04733.

Protocols and materials databases

DNASUi4494.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334350; ENSP00000334872; ENSG00000198417.
GeneIDi4494.
KEGGihsa:4494.
UCSCiuc002ejt.4. human.

Organism-specific databases

CTDi4494.
GeneCardsiMT1F.
HGNCiHGNC:7398. MT1F.
MIMi156352. gene.
neXtProtiNX_P04733.
PharmGKBiPA31203.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IUWR. Eukaryota.
ENOG4110PT3. LUCA.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000043048.
InParanoidiP04733.
KOiK14739.
OMAiKECKCIS.
OrthoDBiEOG7PZS1X.
PhylomeDBiP04733.
TreeFamiTF336054.

Enzyme and pathway databases

ReactomeiR-HSA-5661231. Metallothioneins bind metals.

Miscellaneous databases

ChiTaRSiMT1F. human.
GeneWikiiMT1F.
GenomeRNAii4494.
PROiP04733.
SOURCEiSearch...

Gene expression databases

BgeeiP04733.
CleanExiHS_MT1F.
ExpressionAtlasiP04733. baseline and differential.
GenevisibleiP04733. HS.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR003019. Metalthion.
IPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and expression of two human metallothionein-I isoform genes and a related pseudogene."
    Schmidt C.J., Jubier M.-F., Hamer D.H.
    J. Biol. Chem. 260:7731-7737(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure, organization, and regulation of human metallothionein IF gene: differential and cell-type-specific expression in response to heavy metals and glucocorticoids."
    Varshney U., Jahroudi N., Foster R., Gedamu L.
    Mol. Cell. Biol. 6:26-37(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Structure and expression of the human metallothionein genes."
    Gedamu L., Varshney U., Jahroudi N., Foster R., Shworak N.W.
    Experientia Suppl. 52:361-372(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Functional prediction of the coding sequences of 50 new genes deduced by analysis of cDNA clones from human fetal liver."
    Yu Y., Zhang C., Luo L., Ouyang S., Zhang S., Li W., Wu J., Zhou S., Liu M., He F.
    Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Fetal liver.
  5. Hunziker P.E.
    Submitted (NOV-1993) to UniProtKB
    Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1.
    Tissue: Liver.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMT1F_HUMAN
AccessioniPrimary (citable) accession number: P04733
Secondary accession number(s): Q9UI97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: June 8, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Metallothioneins
    Classification of metallothioneins and list of entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.