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P04732 (MT1E_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metallothionein-1E

Short name=MT-1E
Alternative name(s):
Metallothionein-IE
Short name=MT-IE
Gene names
Name:MT1E
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length61 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Subunit structure

Monomer.

Domain

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similarities

Belongs to the metallothionein superfamily. Type 1 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P04732-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P04732-2)

The sequence of this isoform differs from the canonical sequence as follows:
     33-61: CCSCCPVGCAKCAQGCVCKGASEKCSCCA → ECGAISRNLG...WVLSSSQACY

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6161Metallothionein-1E
PRO_0000197236

Regions

Region1 – 2929Beta
Region30 – 6132Alpha

Sites

Metal binding51Divalent metal cation; cluster B
Metal binding71Divalent metal cation; cluster B
Metal binding131Divalent metal cation; cluster B
Metal binding151Divalent metal cation; cluster B
Metal binding191Divalent metal cation; cluster B
Metal binding211Divalent metal cation; cluster B
Metal binding241Divalent metal cation; cluster B
Metal binding261Divalent metal cation; cluster B
Metal binding291Divalent metal cation; cluster B
Metal binding331Divalent metal cation; cluster A
Metal binding341Divalent metal cation; cluster A
Metal binding361Divalent metal cation; cluster A
Metal binding371Divalent metal cation; cluster A
Metal binding411Divalent metal cation; cluster A
Metal binding441Divalent metal cation; cluster A
Metal binding481Divalent metal cation; cluster A
Metal binding501Divalent metal cation; cluster A
Metal binding571Divalent metal cation; cluster A
Metal binding591Divalent metal cation; cluster A
Metal binding601Divalent metal cation; cluster A

Amino acid modifications

Modified residue11N-acetylmethionine Ref.2

Natural variations

Alternative sequence33 – 6129CCSCC…CSCCA → ECGAISRNLGLWLRLGGNSR LALSASFWGTGLSLPSLPVS FPLQAFCPKFRWGRTAFFSW DTNPNCTPYGFRTELCQTKK SILWVWVLSSSQACY in isoform 2.
VSP_041603

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 8E0A7C54F623A1D2

FASTA616,014
        10         20         30         40         50         60 
MDPNCSCATG GSCTCAGSCK CKECKCTSCK KSCCSCCPVG CAKCAQGCVC KGASEKCSCC 


A 

« Hide

Isoform 2 [UniParc].

Checksum: A2D0802B85A161B6
Show »

FASTA12713,863

References

« Hide 'large scale' references
[1]"Structure and expression of two human metallothionein-I isoform genes and a related pseudogene."
Schmidt C.J., Jubier M.-F., Hamer D.H.
J. Biol. Chem. 260:7731-7737(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 1).
[2]"Amino acid sequence determination."
Hunziker P.E.
Methods Enzymol. 205:421-426(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE (ISOFORM 1), ACETYLATION AT MET-1.
Tissue: Liver.
[3]Guo J.H., Huang X.H., Yu L.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Liver.
[4]Yu L.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
[8]"Induction by zinc of specific metallothionein isoforms in human monocytes."
Pauwels M., van Weyenbergh J., Soumillion A., Proost P., Ley M.
Eur. J. Biochem. 220:105-110(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31 (ISOFORM 1/2).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M10942 Genomic DNA. Translation: AAA59587.1.
AF495759 mRNA. Translation: AAM15968.1.
AF348996 mRNA. Translation: AAO32956.2.
AC026461 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW82872.1.
CH471092 Genomic DNA. Translation: EAW82874.1.
BC009699 mRNA. Translation: AAH09699.1.
BC062734 mRNA. Translation: AAH62734.1.
BC131609 mRNA. Translation: AAI31610.1.
S68949 mRNA. Translation: AAB30082.1.
PIRSMHU1E. A22634.
RefSeqNP_783316.2. NM_175617.3.
XP_005256013.1. XM_005255956.2.
UniGeneHs.744893.

3D structure databases

ProteinModelPortalP04732.
SMRP04732. Positions 1-61.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110599. 1 interaction.
STRING9606.ENSP00000307706.

Proteomic databases

PaxDbP04732.
PRIDEP04732.

Protocols and materials databases

DNASU4493.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000306061; ENSP00000307706; ENSG00000169715. [P04732-1]
ENST00000330439; ENSP00000328137; ENSG00000169715. [P04732-2]
GeneID4493.
KEGGhsa:4493.
UCSCuc002ejl.3. human. [P04732-1]
uc002ejm.3. human. [P04732-2]

Organism-specific databases

CTD4493.
GeneCardsGC16P056659.
H-InvDBHIX0040616.
HGNCHGNC:7397. MT1E.
HPAHPA041152.
MIM156351. gene.
neXtProtNX_P04732.
PharmGKBPA31202.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG138665.
HOGENOMHOG000236262.
KOK14739.
OMACKCASCH.
OrthoDBEOG7PZS1X.
PhylomeDBP04732.
TreeFamTF336054.

Gene expression databases

ArrayExpressP04732.
BgeeP04732.
CleanExHS_MT1E.
GenevestigatorP04732.

Family and domain databases

Gene3D4.10.10.10. 1 hit.
InterProIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERPTHR23299. PTHR23299. 1 hit.
PfamPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSPR00860. MTVERTEBRATE.
SUPFAMSSF57868. SSF57868. 1 hit.
PROSITEPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMT1E.
GenomeRNAi4493.
NextBio17375.
PROP04732.
SOURCESearch...

Entry information

Entry nameMT1E_HUMAN
AccessionPrimary (citable) accession number: P04732
Secondary accession number(s): A2RRF7, Q86YX4, Q8TD51
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 16, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Metallothioneins

Classification of metallothioneins and list of entries

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM