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P04732

- MT1E_HUMAN

UniProt

P04732 - MT1E_HUMAN

Protein

Metallothionein-1E

Gene

MT1E

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi5 – 51Divalent metal cation; cluster B
    Metal bindingi7 – 71Divalent metal cation; cluster B
    Metal bindingi13 – 131Divalent metal cation; cluster B
    Metal bindingi15 – 151Divalent metal cation; cluster B
    Metal bindingi19 – 191Divalent metal cation; cluster B
    Metal bindingi21 – 211Divalent metal cation; cluster B
    Metal bindingi24 – 241Divalent metal cation; cluster B
    Metal bindingi26 – 261Divalent metal cation; cluster B
    Metal bindingi29 – 291Divalent metal cation; cluster B
    Metal bindingi33 – 331Divalent metal cation; cluster A
    Metal bindingi34 – 341Divalent metal cation; cluster A
    Metal bindingi36 – 361Divalent metal cation; cluster A
    Metal bindingi37 – 371Divalent metal cation; cluster A
    Metal bindingi41 – 411Divalent metal cation; cluster A
    Metal bindingi44 – 441Divalent metal cation; cluster A
    Metal bindingi48 – 481Divalent metal cation; cluster A
    Metal bindingi50 – 501Divalent metal cation; cluster A
    Metal bindingi57 – 571Divalent metal cation; cluster A
    Metal bindingi59 – 591Divalent metal cation; cluster A
    Metal bindingi60 – 601Divalent metal cation; cluster A

    GO - Molecular functioni

    1. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to cadmium ion Source: UniProtKB
    2. cellular response to zinc ion Source: UniProtKB
    3. negative regulation of growth Source: UniProtKB

    Keywords - Ligandi

    Cadmium, Copper, Metal-binding, Metal-thiolate cluster, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metallothionein-1E
    Short name:
    MT-1E
    Alternative name(s):
    Metallothionein-IE
    Short name:
    MT-IE
    Gene namesi
    Name:MT1E
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:7397. MT1E.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB
    3. perinuclear region of cytoplasm Source: UniProtKB

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31202.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6161Metallothionein-1EPRO_0000197236Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP04732.
    PaxDbiP04732.
    PRIDEiP04732.

    Expressioni

    Gene expression databases

    ArrayExpressiP04732.
    BgeeiP04732.
    CleanExiHS_MT1E.
    GenevestigatoriP04732.

    Organism-specific databases

    HPAiHPA041152.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi110599. 1 interaction.
    STRINGi9606.ENSP00000307706.

    Structurei

    3D structure databases

    ProteinModelPortaliP04732.
    SMRiP04732. Positions 1-61.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2929BetaAdd
    BLAST
    Regioni30 – 6132AlphaAdd
    BLAST

    Domaini

    Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG138665.
    HOGENOMiHOG000236262.
    KOiK14739.
    OMAiQVIRFAC.
    OrthoDBiEOG7PZS1X.
    PhylomeDBiP04732.
    TreeFamiTF336054.

    Family and domain databases

    Gene3Di4.10.10.10. 1 hit.
    InterProiIPR017854. Metalthion_dom.
    IPR023587. Metalthion_dom_vert.
    IPR003019. Metalthion_sfam_euk.
    IPR000006. Metalthion_vert.
    IPR018064. Metalthion_vert_metal_BS.
    [Graphical view]
    PANTHERiPTHR23299. PTHR23299. 1 hit.
    PfamiPF00131. Metallothio. 1 hit.
    [Graphical view]
    PRINTSiPR00860. MTVERTEBRATE.
    SUPFAMiSSF57868. SSF57868. 1 hit.
    PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P04732-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDPNCSCATG GSCTCAGSCK CKECKCTSCK KSCCSCCPVG CAKCAQGCVC   50
    KGASEKCSCC A 61
    Length:61
    Mass (Da):6,014
    Last modified:August 13, 1987 - v1
    Checksum:i8E0A7C54F623A1D2
    GO
    Isoform 2 (identifier: P04732-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         33-61: CCSCCPVGCAKCAQGCVCKGASEKCSCCA → ECGAISRNLG...WVLSSSQACY

    Show »
    Length:127
    Mass (Da):13,863
    Checksum:iA2D0802B85A161B6
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei33 – 6129CCSCC…CSCCA → ECGAISRNLGLWLRLGGNSR LALSASFWGTGLSLPSLPVS FPLQAFCPKFRWGRTAFFSW DTNPNCTPYGFRTELCQTKK SILWVWVLSSSQACY in isoform 2. 1 PublicationVSP_041603Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10942 Genomic DNA. Translation: AAA59587.1.
    AF495759 mRNA. Translation: AAM15968.1.
    AF348996 mRNA. Translation: AAO32956.2.
    AC026461 Genomic DNA. No translation available.
    CH471092 Genomic DNA. Translation: EAW82872.1.
    CH471092 Genomic DNA. Translation: EAW82874.1.
    BC009699 mRNA. Translation: AAH09699.1.
    BC062734 mRNA. Translation: AAH62734.1.
    BC131609 mRNA. Translation: AAI31610.1.
    S68949 mRNA. Translation: AAB30082.1.
    CCDSiCCDS10764.2. [P04732-1]
    PIRiA22634. SMHU1E.
    RefSeqiNP_783316.2. NM_175617.3. [P04732-1]
    XP_005256013.1. XM_005255956.2. [P04732-2]
    UniGeneiHs.744893.

    Genome annotation databases

    EnsembliENST00000306061; ENSP00000307706; ENSG00000169715. [P04732-1]
    ENST00000330439; ENSP00000328137; ENSG00000169715. [P04732-2]
    GeneIDi4493.
    KEGGihsa:4493.
    UCSCiuc002ejl.3. human. [P04732-1]
    uc002ejm.3. human. [P04732-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M10942 Genomic DNA. Translation: AAA59587.1 .
    AF495759 mRNA. Translation: AAM15968.1 .
    AF348996 mRNA. Translation: AAO32956.2 .
    AC026461 Genomic DNA. No translation available.
    CH471092 Genomic DNA. Translation: EAW82872.1 .
    CH471092 Genomic DNA. Translation: EAW82874.1 .
    BC009699 mRNA. Translation: AAH09699.1 .
    BC062734 mRNA. Translation: AAH62734.1 .
    BC131609 mRNA. Translation: AAI31610.1 .
    S68949 mRNA. Translation: AAB30082.1 .
    CCDSi CCDS10764.2. [P04732-1 ]
    PIRi A22634. SMHU1E.
    RefSeqi NP_783316.2. NM_175617.3. [P04732-1 ]
    XP_005256013.1. XM_005255956.2. [P04732-2 ]
    UniGenei Hs.744893.

    3D structure databases

    ProteinModelPortali P04732.
    SMRi P04732. Positions 1-61.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110599. 1 interaction.
    STRINGi 9606.ENSP00000307706.

    Proteomic databases

    MaxQBi P04732.
    PaxDbi P04732.
    PRIDEi P04732.

    Protocols and materials databases

    DNASUi 4493.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000306061 ; ENSP00000307706 ; ENSG00000169715 . [P04732-1 ]
    ENST00000330439 ; ENSP00000328137 ; ENSG00000169715 . [P04732-2 ]
    GeneIDi 4493.
    KEGGi hsa:4493.
    UCSCi uc002ejl.3. human. [P04732-1 ]
    uc002ejm.3. human. [P04732-2 ]

    Organism-specific databases

    CTDi 4493.
    GeneCardsi GC16P056659.
    H-InvDB HIX0040616.
    HGNCi HGNC:7397. MT1E.
    HPAi HPA041152.
    MIMi 156351. gene.
    neXtProti NX_P04732.
    PharmGKBi PA31202.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG138665.
    HOGENOMi HOG000236262.
    KOi K14739.
    OMAi QVIRFAC.
    OrthoDBi EOG7PZS1X.
    PhylomeDBi P04732.
    TreeFami TF336054.

    Miscellaneous databases

    GeneWikii MT1E.
    GenomeRNAii 4493.
    NextBioi 17375.
    PROi P04732.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P04732.
    Bgeei P04732.
    CleanExi HS_MT1E.
    Genevestigatori P04732.

    Family and domain databases

    Gene3Di 4.10.10.10. 1 hit.
    InterProi IPR017854. Metalthion_dom.
    IPR023587. Metalthion_dom_vert.
    IPR003019. Metalthion_sfam_euk.
    IPR000006. Metalthion_vert.
    IPR018064. Metalthion_vert_metal_BS.
    [Graphical view ]
    PANTHERi PTHR23299. PTHR23299. 1 hit.
    Pfami PF00131. Metallothio. 1 hit.
    [Graphical view ]
    PRINTSi PR00860. MTVERTEBRATE.
    SUPFAMi SSF57868. SSF57868. 1 hit.
    PROSITEi PS00203. METALLOTHIONEIN_VRT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of two human metallothionein-I isoform genes and a related pseudogene."
      Schmidt C.J., Jubier M.-F., Hamer D.H.
      J. Biol. Chem. 260:7731-7737(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 1).
    2. Cited for: PROTEIN SEQUENCE (ISOFORM 1), ACETYLATION AT MET-1.
      Tissue: Liver.
    3. Guo J.H., Huang X.H., Yu L.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Liver.
    4. Yu L.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    5. "The sequence and analysis of duplication-rich human chromosome 16."
      Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
      , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
      Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.
    8. "Induction by zinc of specific metallothionein isoforms in human monocytes."
      Pauwels M., van Weyenbergh J., Soumillion A., Proost P., Ley M.
      Eur. J. Biochem. 220:105-110(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31 (ISOFORM 1/2).

    Entry informationi

    Entry nameiMT1E_HUMAN
    AccessioniPrimary (citable) accession number: P04732
    Secondary accession number(s): A2RRF7, Q86YX4, Q8TD51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Metallothioneins
      Classification of metallothioneins and list of entries
    3. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3