P04732 (MT1E_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metallothionein-1E
Short name=MT-1E

Alternative name(s):
Metallothionein-IE
Short name=MT-IE

Gene names

Name:

MT1E

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Subunit structure	Monomer.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Cadmium Copper Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to cadmium ion Inferred from expression pattern PubMed 16827180. Source: UniProtKB cellular response to zinc ion Inferred from expression pattern PubMed 11168427Ref.8. Source: UniProtKB negative regulation of growth Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from direct assay PubMed 8619255. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cadmium ion binding Non-traceable author statement. Source: UniProtKB copper ion binding Non-traceable author statement. Source: UniProtKB zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P04732-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P04732-2)

The sequence of this isoform differs from the canonical sequence as follows:
     33-61: CCSCCPVGCAKCAQGCVCKGASEKCSCCA → ECGAISRNLG...WVLSSSQACY

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Metallothionein-1E		PRO_0000197236
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Divalent metal cation; cluster B
	Metal binding	7	1	Divalent metal cation; cluster B
	Metal binding	13	1	Divalent metal cation; cluster B
	Metal binding	15	1	Divalent metal cation; cluster B
	Metal binding	19	1	Divalent metal cation; cluster B
	Metal binding	21	1	Divalent metal cation; cluster B
	Metal binding	24	1	Divalent metal cation; cluster B
	Metal binding	26	1	Divalent metal cation; cluster B
	Metal binding	29	1	Divalent metal cation; cluster B
	Metal binding	33	1	Divalent metal cation; cluster A
	Metal binding	34	1	Divalent metal cation; cluster A
	Metal binding	36	1	Divalent metal cation; cluster A
	Metal binding	37	1	Divalent metal cation; cluster A
	Metal binding	41	1	Divalent metal cation; cluster A
	Metal binding	44	1	Divalent metal cation; cluster A
	Metal binding	48	1	Divalent metal cation; cluster A
	Metal binding	50	1	Divalent metal cation; cluster A
	Metal binding	57	1	Divalent metal cation; cluster A
	Metal binding	59	1	Divalent metal cation; cluster A
	Metal binding	60	1	Divalent metal cation; cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.2
Natural variations
	Alternative sequence	33 – 61	29	CCSCC…CSCCA → ECGAISRNLGLWLRLGGNSR LALSASFWGTGLSLPSLPVS FPLQAFCPKFRWGRTAFFSW DTNPNCTPYGFRTELCQTKK SILWVWVLSSSQACY in isoform 2.		VSP_041603

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 8E0A7C54F623A1D2	FASTA	61	6,014
	10 20 30 40 50 60 MDPNCSCATG GSCTCAGSCK CKECKCTSCK KSCCSCCPVG CAKCAQGCVC KGASEKCSCC A
	Isoform 2 [UniParc]. Checksum: A2D0802B85A161B6	FASTA	127	13,863
	10 20 30 40 50 60 MDPNCSCATG GSCTCAGSCK CKECKCTSCK KSECGAISRN LGLWLRLGGN SRLALSASFW 70 80 90 100 110 120 GTGLSLPSLP VSFPLQAFCP KFRWGRTAFF SWDTNPNCTP YGFRTELCQT KKSILWVWVL SSSQACY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of two human metallothionein-I isoform genes and a related pseudogene." Schmidt C.J., Jubier M.-F., Hamer D.H. J. Biol. Chem. 260:7731-7737(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 1).
[2]	"Amino acid sequence determination." Hunziker P.E. Methods Enzymol. 205:421-426(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE (ISOFORM 1), ACETYLATION AT MET-1. Tissue: Liver.
[3]	Guo J.H., Huang X.H., Yu L. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Liver.
[4]	Yu L. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[8]	"Induction by zinc of specific metallothionein isoforms in human monocytes." Pauwels M., van Weyenbergh J., Soumillion A., Proost P., Ley M. Eur. J. Biochem. 220:105-110(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31 (ISOFORM 1/2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M10942 Genomic DNA. Translation: AAA59587.1. AF495759 mRNA. Translation: AAM15968.1. AF348996 mRNA. Translation: AAO32956.2. AC026461 Genomic DNA. No translation available. CH471092 Genomic DNA. Translation: EAW82872.1. CH471092 Genomic DNA. Translation: EAW82874.1. BC009699 mRNA. Translation: AAH09699.1. BC062734 mRNA. Translation: AAH62734.1. BC131609 mRNA. Translation: AAI31610.1. S68949 mRNA. Translation: AAB30082.1.
IPI	IPI00025461. IPI00152456.
PIR	SMHU1E. A22634.
RefSeq	NP_783316.2. NM_175617.3.
UniGene	Hs.534330.
3D structure databases
ProteinModelPortal	P04732.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000307706.
Polymorphism databases
DMDM	127370.
Proteomic databases
PaxDb	P04732.
PRIDE	P04732.
Protocols and materials databases
DNASU	4493.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306061; ENSP00000307706; ENSG00000169715. ENST00000330439; ENSP00000328137; ENSG00000169715.
GeneID	4493.
KEGG	hsa:4493.
UCSC	uc002ejl.3. human. uc002ejm.3. human.
Organism-specific databases
CTD	4493.
GeneCards	GC16P056659.
H-InvDB	HIX0040616.
HGNC	HGNC:7397. MT1E.
HPA	HPA041152.
MIM	156351. gene.
neXtProt	NX_P04732.
PharmGKB	PA31202.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG138665.
HOGENOM	HOG000236262.
KO	K14739.
OMA	SCACKNC.
OrthoDB	EOG46WZB6.
Gene expression databases
ArrayExpress	P04732.
Bgee	P04732.
CleanEx	HS_MT1E.
Genevestigator	P04732.
GermOnline	ENSG00000169715. Homo sapiens.
Family and domain databases
Gene3D	4.10.10.10. 1 hit.
InterPro	IPR017854. Metalthion_dom. IPR023587. Metalthion_dom_vert. IPR003019. Metalthion_sfam_euk. IPR000006. Metalthion_vert. IPR018064. Metalthion_vert_metal_BS. [Graphical view]
PANTHER	PTHR23299. PTHR23299. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
SUPFAM	SSF57868. Metallothionein_sfam. 1 hit.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4493.
NextBio	17375.
SOURCE	Search...

Entry information

Entry name

MT1E_HUMAN

Accession

Primary (citable) accession number: P04732
Secondary accession number(s): A2RRF7, Q86YX4, Q8TD51

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	May 1, 2013
This is version 125 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.