P04706 (GLU2_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutelin-2 Alternative name(s): 27 kDa zein Alcohol-soluble reduced glutelin Short name=ASG Zein Zc2 Zein-gamma
Organism	Zea mays (Maize)
Taxonomic identifier	4577 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seed storage protein. It accounts for about 15% of the total endosperm protein content.
Subcellular location	Vacuole › aleurone grain membrane. Note: Border of the inner part of the membrane of endosperm protein bodies.
Sequence similarities	Belongs to the gliadin/glutenin family.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Repeat Signal
Molecular function	Seed storage protein Storage protein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	aleurone grain membrane Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.6
	Chain	20 – 223	204	Glutelin-2		PRO_0000032214
Regions
	Repeat	31 – 36	6	1-1
	Repeat	37 – 42	6	1-2
	Repeat	43 – 48	6	1-3
	Repeat	49 – 54	6	1-4
	Repeat	55 – 60	6	1-5
	Repeat	61 – 66	6	1-6
	Repeat	67 – 72	6	1-7
	Repeat	73 – 78	6	1-8
	Repeat	97 – 104	8	2-1
	Repeat	105 – 112	8	2-2
	Region	31 – 78	48	8 X 6 AA tandem repeats of P-P-P-V-H-L
	Region	97 – 112	16	2 X 8 AA tandem repeats of Q-P-H-P-C-P-C-Q
Natural variations
	Natural variant	28	1	C → S in 66% of the molecules. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P04706 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 3E80C06B2DB58372	FASTA	223	23,689
	10 20 30 40 50 60 MRVLLVALAL LALAASATST HTSGGCGCQP PPPVHLPPPV HLPPPVHLPP PVHLPPPVHL 70 80 90 100 110 120 PPPVHLPPPV HVPPPVHLPP PPCHYPTQPP RPQPHPQPHP CPCQQPHPSP CQLQGTCGVG 130 140 150 160 170 180 STPILGQCVE FLRHQCSPTA TPYCSPQCQS LRQQCCQQLR QVEPQHRYQA IFGLVLQSIL 190 200 210 220 QQQPQSGQVA GLLAAQIAQQ LTAMCGLQQP TPCPYAAAGG VPH

References

[1]	"Multiple variability in the sequence of a family of maize endosperm proteins." Prat S., Perez-Grau L., Puigdomenech P. Gene 52:41-49(1987) [PubMed: 3596247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. W64 X W64O2.
[2]	"Nucleic acid (cDNA) and amino acid sequences of the maize endosperm protein glutelin-2." Prat S., Cortadas J., Puigdomenech P., Palau J. Nucleic Acids Res. 13:1493-1504(1985) [PubMed: 3839076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. inbred line E-10.
[3]	"Isolation and sequencing of a 28 kd glutelin-2 gene from maize: common elements in the 5' flanking regions among zein and glutelin genes." Boronat A., Martinez M.C., Reina M., Puigdomenech P., Palau J. Plant Sci. 47:95-102(1986) Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Sequence analysis of a genomic clone encoding a Zc2 protein from Zea mays W64 A." Reina M., Ponte I., Guillen P., Boronat A., Palau J. Nucleic Acids Res. 18:6426-6426(1990) [PubMed: 2243788] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Wisconsin 64A. Tissue: Endosperm.
[5]	"Primary structure of a proline-rich zein and its cDNA." Wang S.-Z., Esen A. Plant Physiol. 81:70-74(1986) [PubMed: 16664810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 15-223, VARIANT SER-28.
[6]	"Tandem repeats in the N-terminal sequence of a proline-rich protein from corn endosperm." Esen A., Bietz J.A., Paulis J.W., Wall J.S. Nature 296:678-679(1982) Cited for: PROTEIN SEQUENCE OF 20-77.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02230 mRNA. Translation: CAA26149.1. M16066 Genomic DNA. Translation: AAA33468.1. M16218 mRNA. Translation: AAA33537.1. X53514 Genomic DNA. Translation: CAA37594.1. S78780 mRNA. Translation: AAP32017.1.
PIR	ZMZM5. A03351. ZMZM19. A93557.
RefSeq	NP_001105354.1. NM_001111884.1.
UniGene	Zm.102795. Zm.97202.
3D structure databases
ProteinModelPortal	P04706.
ModBase	Search...
Protein family/group databases
Allergome	2780. Zea m 27kD Zein.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	GRMZM2G138727_T01; GRMZM2G138727_P01; GRMZM2G138727.
GeneID	542296.
KEGG	zma:542296.
Organism-specific databases
Gramene	P04706.
MaizeGDB	58111.
Family and domain databases
InterPro	IPR016140. Bifunc_inhib/LTP/seed_store. IPR000480. Glutelin. IPR003612. LTP/seed_store/tryp_amyl_inhib. IPR013771. Trypsin/amylase_inhib. [Graphical view]
Gene3D	G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
PRINTS	PR00211. GLUTELIN.
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLU2_MAIZE

Accession

Primary (citable) accession number: P04706
Secondary accession number(s): P02860, P08415

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	September 21, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program