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P04694

- ATTY_RAT

UniProt

P04694 - ATTY_RAT

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Protein

Tyrosine aminotransferase

Gene

Tat

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has much lower affinity and transaminase activity towards phenylalanine.1 Publication

Catalytic activityi

L-tyrosine + 2-oxoglutarate = 4-hydroxyphenylpyruvate + L-glutamate.1 Publication

Cofactori

Pyridoxal phosphate.2 Publications

Kineticsi

  1. KM=2.2 mM for tyrosine1 Publication

Pathwayi

GO - Molecular functioni

  1. amino acid binding Source: RGD
  2. L-phenylalanine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
  3. L-tyrosine:2-oxoglutarate aminotransferase activity Source: UniProtKB
  4. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. 2-oxoglutarate metabolic process Source: Ensembl
  2. cellular amino acid metabolic process Source: RGD
  3. gluconeogenesis Source: RGD
  4. glutamate metabolic process Source: UniProtKB
  5. L-phenylalanine catabolic process Source: UniProtKB-UniPathway
  6. response to glucocorticoid Source: RGD
  7. response to mercury ion Source: RGD
  8. response to organic cyclic compound Source: RGD
  9. response to oxidative stress Source: RGD
  10. tyrosine catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15101.
BRENDAi2.6.1.5. 5301.
ReactomeiREACT_204731. Phenylalanine and tyrosine catabolism.
SABIO-RKP04694.
UniPathwayiUPA00139; UER00338.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine aminotransferase (EC:2.6.1.5)
Short name:
TAT
Alternative name(s):
L-tyrosine:2-oxoglutarate aminotransferase
Gene namesi
Name:Tat
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 19

Organism-specific databases

RGDi3820. Tat.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Tyrosine aminotransferasePRO_0000123889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei280 – 2801N6-(pyridoxal phosphate)lysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP04694.

PTM databases

PhosphoSiteiP04694.

Expressioni

Tissue specificityi

Liver.

Gene expression databases

GenevestigatoriP04694.

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP04694.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0436.
GeneTreeiENSGT00650000093238.
HOGENOMiHOG000239005.
HOVERGENiHBG004318.
InParanoidiP04694.
KOiK00815.
OMAiHCAEGSQ.
OrthoDBiEOG7PVWP7.
PhylomeDBiP04694.
TreeFamiTF105999.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR011715. Tyr_aminoTrfase_ubiquitination.
IPR005958. TyrNic_aminoTrfase.
IPR005957. Tyrosine_aminoTrfase.
IPR021178. Tyrosine_transaminase.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF07706. TAT_ubiq. 1 hit.
[Graphical view]
PIRSFiPIRSF000517. Tyr_transaminase. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01264. tyr_amTase_E. 1 hit.
TIGR01265. tyr_nico_aTase. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04694-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSYVIQTDV DDSLSSVLDV HVNIGGRNSV QGRKKGRKAR WDVRPSDMSN
60 70 80 90 100
KTFNPIRAIV DNMKVQPNPN KTVISLSIGD PTVFGNLPTD PEVTQAMKDA
110 120 130 140 150
LDSGKYNGYA PSIGYLSSRE EVASYYHCHE APLEAKDVIL TSGCSQAIEL
160 170 180 190 200
CLAVLANPGQ NILIPRPGFS LYRTLAESMG IEVKLYNLLP EKSWEIDLKQ
210 220 230 240 250
LESLIDEKTA CLVVNNPSNP CGSVFSKRHL QKILAVAERQ CVPILADEIY
260 270 280 290 300
GDMVFSDCKY EPLANLSTNV PILSCGGLAK RWLVPGWRLG WILIHDRRDI
310 320 330 340 350
FGNEIRDGLV KLSQRILGPC TIVQGALKSI LQRTPQEFYH DTLSFLKSNA
360 370 380 390 400
DLCYGALAAI PGLQPVRPSG AMYLMVGIEM EHFPEFENDV EFTERLIAEQ
410 420 430 440 450
AVHCLPATCF EYPNFFRVVI TVPEVMMLEA CSRIQEFCEQ HYHCAEGSQE

ECDK
Length:454
Mass (Da):50,635
Last modified:August 13, 1987 - v1
Checksum:i835EBE0D2476AF71
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 602IV → KT in AAA42203. (PubMed:2562840)Curated
Sequence conflicti209 – 2102TA → LE in AAA42203. (PubMed:2562840)Curated
Sequence conflicti285 – 2851P → L in CAA33725. (PubMed:1983704)Curated
Sequence conflicti304 – 3041Missing in CAA33725. (PubMed:1983704)Curated
Sequence conflicti359 – 3602AI → DL in AAA42203. (PubMed:2562840)Curated
Sequence conflicti445 – 4451A → D in AAA42203. (PubMed:2562840)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02741 mRNA. Translation: CAA26519.1.
M18340 mRNA. Translation: AAA42203.1.
BC089813 mRNA. Translation: AAH89813.1.
X15690 Genomic DNA. Translation: CAA33725.1.
PIRiA23310. XNRTY.
RefSeqiNP_036800.1. NM_012668.2.
UniGeneiRn.9947.

Genome annotation databases

EnsembliENSRNOT00000022721; ENSRNOP00000022721; ENSRNOG00000016348.
GeneIDi24813.
KEGGirno:24813.
UCSCiRGD:3820. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02741 mRNA. Translation: CAA26519.1 .
M18340 mRNA. Translation: AAA42203.1 .
BC089813 mRNA. Translation: AAH89813.1 .
X15690 Genomic DNA. Translation: CAA33725.1 .
PIRi A23310. XNRTY.
RefSeqi NP_036800.1. NM_012668.2.
UniGenei Rn.9947.

3D structure databases

ProteinModelPortali P04694.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P04694.
ChEMBLi CHEMBL5947.

PTM databases

PhosphoSitei P04694.

Proteomic databases

PRIDEi P04694.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000022721 ; ENSRNOP00000022721 ; ENSRNOG00000016348 .
GeneIDi 24813.
KEGGi rno:24813.
UCSCi RGD:3820. rat.

Organism-specific databases

CTDi 6898.
RGDi 3820. Tat.

Phylogenomic databases

eggNOGi COG0436.
GeneTreei ENSGT00650000093238.
HOGENOMi HOG000239005.
HOVERGENi HBG004318.
InParanoidi P04694.
KOi K00815.
OMAi HCAEGSQ.
OrthoDBi EOG7PVWP7.
PhylomeDBi P04694.
TreeFami TF105999.

Enzyme and pathway databases

UniPathwayi UPA00139 ; UER00338 .
BioCyci MetaCyc:MONOMER-15101.
BRENDAi 2.6.1.5. 5301.
Reactomei REACT_204731. Phenylalanine and tyrosine catabolism.
SABIO-RK P04694.

Miscellaneous databases

NextBioi 604502.
PROi P04694.

Gene expression databases

Genevestigatori P04694.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR004839. Aminotransferase_I/II.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR011715. Tyr_aminoTrfase_ubiquitination.
IPR005958. TyrNic_aminoTrfase.
IPR005957. Tyrosine_aminoTrfase.
IPR021178. Tyrosine_transaminase.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
PF07706. TAT_ubiq. 1 hit.
[Graphical view ]
PIRSFi PIRSF000517. Tyr_transaminase. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR01264. tyr_amTase_E. 1 hit.
TIGR01265. tyr_nico_aTase. 1 hit.
PROSITEi PS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete complementary DNA of rat tyrosine aminotransferase messenger RNA. Deduction of the primary structure of the enzyme."
    Grange T., Guenet C., Dietrich J.-B., Chasserot S., Fromont M., Befort N., Jami J., Beck G., Pictet R.
    J. Mol. Biol. 184:347-350(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ACETYLATION AT MET-1.
  2. "The structure of tyrosine aminotransferase. Evidence for domains involved in catalysis and enzyme turnover."
    Hargrove J.L., Scoble H.A., Mathews W.R., Baumstark B.R., Biemann K.
    J. Biol. Chem. 264:45-53(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, COFACTOR, IDENTIFICATION BY MASS SPECTROMETRY.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  4. "Nucleotide sequence of rat liver tyrosine aminotransferase gene fragment."
    Morozov I.V.
    DNA Seq. 1:151-155(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 190-386.
    Tissue: Liver.
  5. "Isolation and characterization of active N-terminal truncated apo- and holoenzyme of mammalian liver tyrosine aminotransferase."
    Lorber B., Dietrich J.-B., Kern D.
    FEBS Lett. 291:345-349(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
  6. "Structural and immunochemical properties of rat liver tyrosine aminotransferase."
    Dietrich J.-B., Genot G., Beck G.
    Biochimie 70:673-679(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURAL PROPERTIES.

Entry informationi

Entry nameiATTY_RAT
AccessioniPrimary (citable) accession number: P04694
Secondary accession number(s): Q5EBB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 1, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3