P04653 (CASA1_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-S1-casein

Gene names

Name:

CSN1S1

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important role in the capacity of milk to transport calcium phosphate.
Subcellular location	Secreted.
Tissue specificity	Mammary gland specific. Secreted in milk.
Polymorphism	At least four alleles are known. The sequence shown is that of allele A. The frequency of variant Thr-209 is 0.263, 0.122 and 0.191 in Italian breeds Comisana, Sarda and Sopravissana, respectively.
Sequence similarities	Belongs to the alpha-casein family.
Mass spectrometry	Isoform 1: Molecular mass is 23638.14±3.0 Da from positions 16 - 214. Determined by ESI. Allele A, with 11 phosphate groups. Ref.2 Isoform 2: Molecular mass is 22620.0±1.6 Da from positions 16 - 214. Determined by ESI. Allele A, with 11 phosphate groups. Ref.2 Isoform 1: Molecular mass is 23561.8±2.3 Da from positions 16 - 214. Determined by ESI. Allele C, with 10 phosphate groups. Ref.2 Isoform 2: Molecular mass is 22551.8±2.2 Da from positions 16 - 214. Determined by ESI. Allele C, with 10 phosphate groups. Ref.2 Isoform 1: Molecular mass is 23290.2±2.9 Da from positions 16 - 214. Determined by ESI. Allele D, with 6 phosphate groups. Ref.2 Isoform 2: Molecular mass is 22629.0±1.6 Da from positions 16 - 214. Determined by ESI. Allele D, with 6 phosphate groups. Ref.2

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Milk protein
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P04653-2)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P04653-1)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     156-163: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Ref.2
	Chain	16 – 214	199	Alpha-S1-casein		PRO_0000004459
Regions
	Region	105 – 111	7	Opioid-like peptide sequence
Amino acid modifications
	Modified residue	27	1	Phosphoserine; in allele A Ref.2
	Modified residue	56	1	Phosphoserine; in allele C Ref.2
	Modified residue	61	1	Phosphoserine Ref.2
	Modified residue	63	1	Phosphoserine Ref.2
	Modified residue	79	1	Phosphoserine; in alleles A and C Ref.2
	Modified residue	80	1	Phosphoserine Ref.2
	Modified residue	81	1	Phosphoserine; in alleles A and C Ref.2
	Modified residue	82	1	Phosphoserine Ref.2
	Modified residue	83	1	Phosphoserine; in alleles A and C Ref.2
	Modified residue	90	1	Phosphoserine Ref.2
	Modified residue	130	1	Phosphoserine Ref.2
Natural variations
	Alternative sequence	156 – 163	8	Missing in isoform 2.		VSP_012408
	Natural variant	28	1	S → P in allele C and allele D.
	Natural variant	83	1	S → N in allele D.
	Natural variant	209	1	I → T. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified January 4, 2005. Version 3. Checksum: 9312FBFFBD6E49DC	FASTA	214	24,306
	10 20 30 40 50 60 MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE NINELSKDIG 70 80 90 100 110 120 SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED VPSERYLGYL EQLLRLKKYN 130 140 150 160 170 180 VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM IAVNQELAYF YPQLFRQFYQ LDAYPSGAWY 190 200 210 YLPLGTQYTD APSFSDIPNP IGSENSGKIT MPLW
	Isoform 2 (Short) [UniParc]. Checksum: E6965F9A79597B9F	FASTA	206	23,294
	10 20 30 40 50 60 MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE NINELSKDIG 70 80 90 100 110 120 SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED VPSERYLGYL EQLLRLKKYN 130 140 150 160 170 180 VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM IAVNQLFRQF YQLDAYPSGA WYYLPLGTQY 190 200 TDAPSFSDIP NPIGSENSGK ITMPLW

References

[1]	"Construction and identification of recombinant plasmids carrying cDNAs coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and beta-lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA." Mercier J.-C., Gaye P., Soulier S., Hue-Delahaie D., Vilotte J.-L. Biochimie 67:959-971(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (C ALLELE; ISOFORM 2).
[2]	"Primary structure of ovine alpha s1-caseins: localization of phosphorylation sites and characterization of genetic variants A, C and D." Ferranti P., Malorni A., Nitti G., Laezza P., Pizzano R., Chianese L., Addeo F. J. Dairy Res. 62:281-296(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-214 (A; C AND D ALLELES; ISOFORMS 1 AND 2), PHOSPHORYLATION AT SER-27; SER-56; SER-61; SER-63; SER-79; SER-80; SER-81; SER-82; SER-83; SER-90 AND SER-130, MASS SPECTROMETRY. Tissue: Milk.
[3]	"Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism." Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E., Caroli A. J. Dairy Sci. 87:2606-2613(2004) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT THR-209. Strain: Comisana, Sarda and Sopravissana. Tissue: Blood.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03237 mRNA. Translation: CAA26982.1. AY444506 Genomic DNA. Translation: AAS17944.1.
PIR	KASHS1. A25069.
RefSeq	NP_001009795.1. NM_001009795.1.
UniGene	Oar.22214. Oar.22215. Oar.429.
3D structure databases
ModBase	Search...
Protein family/group databases
Allergome	1241. Ovi a 8. 2962. Ovi a 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	443382.
Organism-specific databases
CTD	1446.
Phylogenomic databases
HOVERGEN	HBG005242.
Family and domain databases
InterPro	IPR026999. Alpha-s1_casein. IPR001588. Casein. [Graphical view]
PANTHER	PTHR10240. PTHR10240. 1 hit.
Pfam	PF00363. Casein. 1 hit. [Graphical view]
PROSITE	PS00306. CASEIN_ALPHA_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASA1_SHEEP

Accession

Primary (citable) accession number: P04653
Secondary accession number(s): Q69B23, Q9TS03, Q9TS48

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 4, 2005
Last modified:	May 29, 2013
This is version 72 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program