Reviewed,
UniProtKB/Swiss-Prot P04653 (CASA1_SHEEP)
Last modified
July 22, 2008.
Version 60.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Alpha-S1-casein | ||
| Gene names |
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| Organism | Ovis aries (Sheep) | ||
| Taxonomic identifier | 9940 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis |
Protein attributes
| Sequence length | 214 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Important role in the capacity of milk to transport calcium phosphate. |
| Subcellular location | |
| Tissue specificity | Mammary gland specific. Secreted in milk. |
| Polymorphism | At least four alleles are known. The sequence shown is that of allele A. The frequency of variant Thr-209 is 0.263, 0.122 and 0.191 in Italian breeds Comisana, Sarda and Sopravissana, respectively. |
| Sequence similarities | Belongs to the alpha-casein family. |
| Mass spectrometry | Molecular weight is 23638.14±3.0 Da from positions 16 - 214 (P04653-2). Determined by ESI. Allele A, with 11 phosphate groups Molecular weight is 22620.0±1.6 Da from positions 16 - 214 (P04653-1). Determined by ESI. Allele A, with 11 phosphate groups Molecular weight is 23561.8±2.3 Da from positions 16 - 214 (P04653-2). Determined by ESI. Allele C, with 10 phosphate groups Molecular weight is 22551.8±2.2 Da from positions 16 - 214 (P04653-1). Determined by ESI. Allele C, with 10 phosphate groups Molecular weight is 23290.2±2.9 Da from positions 16 - 214 (P04653-2). Determined by ESI. Allele D, with 6 phosphate groups Molecular weight is 22629.0±1.6 Da from positions 16 - 214 (P04653-1). Determined by ESI. Allele D, with 6 phosphate groups |
Ontologies
Keywords | |
|---|---|
| Cellular component | Secreted |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Domain | Signal |
| PTM | Phosphoprotein |
| Technical term | Direct protein sequencing |
Gene Ontology (GO) | |
| None. [Check GOA] | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | |||||
| Isoform 1 (identifier: P04653-2) Also known as: Long; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | |||||
| Notes: Allele A, with 11 phosphate groups Allele C, with 10 phosphate groups Allele D, with 6 phosphate groups | |||||
| Isoform 2 (identifier: P04653-1) Also known as: Short; The sequence of this isoform differs from the canonical sequence as follows: 156-163: Missing. | |||||
| Notes: Allele A, with 11 phosphate groups Allele C, with 10 phosphate groups Allele D, with 6 phosphate groups |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | ||||
Molecule processing | ||||||||
|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 15 | 15 | ||||||
| Chain | 16 – 214 | 199 | Alpha-S1-casein | |||||
Regions | ||||||||
| Region | 105 – 111 | 7 | Opioid-like peptide sequence | |||||
Amino acid modifications | ||||||||
| Modified residue | 27 | 1 | Phosphoserine; in allele A | |||||
| Modified residue | 56 | 1 | Phosphoserine; in allele C | |||||
| Modified residue | 61 | 1 | Phosphoserine | |||||
| Modified residue | 63 | 1 | Phosphoserine | |||||
| Modified residue | 79 | 1 | Phosphoserine; in alleles A and C | |||||
| Modified residue | 80 | 1 | Phosphoserine | |||||
| Modified residue | 81 | 1 | Phosphoserine; in alleles A and C | |||||
| Modified residue | 82 | 1 | Phosphoserine | |||||
| Modified residue | 83 | 1 | Phosphoserine; in alleles A and C | |||||
| Modified residue | 90 | 1 | Phosphoserine | |||||
| Modified residue | 130 | 1 | Phosphoserine | |||||
Natural variations | ||||||||
| Alternative sequence | 156 – 163 | 8 | Missing in isoform 2. | |||||
| Natural variant | 28 | 1 | S → P in allele C and allele D. | |||||
| Natural variant | 83 | 1 | S → N in allele D. | |||||
| Natural variant | 209 | 1 | I → T | |||||
Sequences
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References
| [1] | "Construction and identification of recombinant plasmids carrying cDNAs coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and beta-lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA." Mercier J.-C., Gaye P., Soulier S., Hue-Delahaie D., Vilotte J.-L. Biochimie 67:959-971(1985) [PubMed: 3002501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (C ALLELE; ISOFORM 2). |
| [2] | "Primary structure of ovine alpha s1-caseins: localization of phosphorylation sites and characterization of genetic variants A, C and D." Ferranti P., Malorni A., Nitti G., Laezza P., Pizzano R., Chianese L., Addeo F. J. Dairy Res. 62:281-296(1995) [PubMed: 7601973] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-214 (A; C AND D ALLELES; ISOFORMS 1 AND 2), PHOSPHORYLATION AT SER-27; SER-56; SER-61; SER-63; SER-79; SER-80; SER-81; SER-82; SER-83; SER-90 AND SER-130, MASS SPECTROMETRY. Tissue: Milk. |
| [3] | "Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism." Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E., Caroli A. J. Dairy Sci. 87:2606-2613(2004) [PubMed: 15328285] [Abstract] Cited for: VARIANT THR-209. Strain: Comisana, Sarda and Sopravissana. Tissue: Blood. |
Cross-references
Sequence databases | |
|---|---|
| X03237 mRNA. Translation: CAA26982.1. AY444506 Genomic DNA. Translation: AAS17944.1. | |
| PIR | KASHS1. A25069. |
| RefSeq | NP_001009795.1. |
| UniGene | Oar.429 |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 443382. |
Phylogenomic databases | |
| HOVERGEN | P04653. |
Family and domain databases | |
| InterPro | IPR001588. Casein. [Graphical view] |
| Pfam | PF00363. Casein. 1 hit. [Graphical view] |
| PROSITE | PS00306. CASEIN_ALPHA_BETA. 1 hit. [Graphical view] |
| ProDom | P04653. [Graphical view] [Entries sharing at least one domain] |
| BLOCKS | Search... |
Other Resources | |
| ProtoNet | Search... |
Entry information
| Entry name | CASA1_SHEEP | ||||||||
| Accession | Primary (citable) accession number: P04653 Secondary accession number(s): Q69B23, Q9TS03, Q9TS48 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |
| Recent format changes Overview of recent format changes |

Clusters with


