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P04653

- CASA1_SHEEP

UniProt

P04653 - CASA1_SHEEP

Protein

Alpha-S1-casein

Gene

CSN1S1

Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 3 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Important role in the capacity of milk to transport calcium phosphate.

    GO - Molecular functioni

    1. transporter activity Source: InterPro

    Keywords - Molecular functioni

    Milk protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-S1-casein
    Gene namesi
    Name:CSN1S1
    OrganismiOvis aries (Sheep)
    Taxonomic identifieri9940 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
    ProteomesiUP000002356: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Protein family/group databases

    Allergomei1241. Ovi a 8.
    2962. Ovi a 9.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 15151 PublicationAdd
    BLAST
    Chaini16 – 214199Alpha-S1-caseinPRO_0000004459Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei27 – 271Phosphoserine; in allele A1 Publication
    Modified residuei56 – 561Phosphoserine; in allele C1 Publication
    Modified residuei61 – 611Phosphoserine1 Publication
    Modified residuei63 – 631Phosphoserine1 Publication
    Modified residuei79 – 791Phosphoserine; in alleles A and C1 Publication
    Modified residuei80 – 801Phosphoserine1 Publication
    Modified residuei81 – 811Phosphoserine; in alleles A and C1 Publication
    Modified residuei82 – 821Phosphoserine1 Publication
    Modified residuei83 – 831Phosphoserine; in alleles A and C1 Publication
    Modified residuei90 – 901Phosphoserine1 Publication
    Modified residuei130 – 1301Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Mammary gland specific. Secreted in milk.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni105 – 1117Opioid-like peptide sequence

    Sequence similaritiesi

    Belongs to the alpha-casein family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG005242.

    Family and domain databases

    InterProiIPR026999. Alpha-s1_casein.
    IPR001588. Casein.
    [Graphical view]
    PANTHERiPTHR10240. PTHR10240. 1 hit.
    PfamiPF00363. Casein. 1 hit.
    [Graphical view]
    PROSITEiPS00306. CASEIN_ALPHA_BETA. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P04653-2) [UniParc]FASTAAdd to Basket

    Also known as: Long

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE    50
    NINELSKDIG SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED 100
    VPSERYLGYL EQLLRLKKYN VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM 150
    IAVNQELAYF YPQLFRQFYQ LDAYPSGAWY YLPLGTQYTD APSFSDIPNP 200
    IGSENSGKIT MPLW 214
    Length:214
    Mass (Da):24,306
    Last modified:January 4, 2005 - v3
    Checksum:i9312FBFFBD6E49DC
    GO
    Isoform 2 (identifier: P04653-1) [UniParc]FASTAAdd to Basket

    Also known as: Short

    The sequence of this isoform differs from the canonical sequence as follows:
         156-163: Missing.

    Show »
    Length:206
    Mass (Da):23,294
    Checksum:iE6965F9A79597B9F
    GO

    Mass spectrometryi

    Isoform 1 : Molecular mass is 23638.14±3.0 Da from positions 16 - 214. Determined by ESI. Allele A, with 11 phosphate groups.1 Publication
    Isoform 2 : Molecular mass is 22620.0±1.6 Da from positions 16 - 214. Determined by ESI. Allele A, with 11 phosphate groups.1 Publication
    Isoform 1 : Molecular mass is 23561.8±2.3 Da from positions 16 - 214. Determined by ESI. Allele C, with 10 phosphate groups.1 Publication
    Isoform 2 : Molecular mass is 22551.8±2.2 Da from positions 16 - 214. Determined by ESI. Allele C, with 10 phosphate groups.1 Publication
    Isoform 1 : Molecular mass is 23290.2±2.9 Da from positions 16 - 214. Determined by ESI. Allele D, with 6 phosphate groups.1 Publication
    Isoform 2 : Molecular mass is 22629.0±1.6 Da from positions 16 - 214. Determined by ESI. Allele D, with 6 phosphate groups.1 Publication

    Polymorphismi

    At least four alleles are known. The sequence shown is that of allele A. The frequency of variant Thr-209 is 0.263, 0.122 and 0.191 in Italian breeds Comisana, Sarda and Sopravissana, respectively.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti28 – 281S → P in allele C and allele D.
    Natural varianti83 – 831S → N in allele D.
    Natural varianti209 – 2091I → T.1 Publication

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei156 – 1638Missing in isoform 2. CuratedVSP_012408

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03237 mRNA. Translation: CAA26982.1.
    AY444506 Genomic DNA. Translation: AAS17944.1.
    PIRiA25069. KASHS1.
    RefSeqiNP_001009795.1. NM_001009795.1.
    UniGeneiOar.22214.
    Oar.22215.
    Oar.429.

    Genome annotation databases

    GeneIDi443382.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03237 mRNA. Translation: CAA26982.1 .
    AY444506 Genomic DNA. Translation: AAS17944.1 .
    PIRi A25069. KASHS1.
    RefSeqi NP_001009795.1. NM_001009795.1.
    UniGenei Oar.22214.
    Oar.22215.
    Oar.429.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 1241. Ovi a 8.
    2962. Ovi a 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 443382.

    Organism-specific databases

    CTDi 1446.

    Phylogenomic databases

    HOVERGENi HBG005242.

    Family and domain databases

    InterProi IPR026999. Alpha-s1_casein.
    IPR001588. Casein.
    [Graphical view ]
    PANTHERi PTHR10240. PTHR10240. 1 hit.
    Pfami PF00363. Casein. 1 hit.
    [Graphical view ]
    PROSITEi PS00306. CASEIN_ALPHA_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Construction and identification of recombinant plasmids carrying cDNAs coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and beta-lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA."
      Mercier J.-C., Gaye P., Soulier S., Hue-Delahaie D., Vilotte J.-L.
      Biochimie 67:959-971(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (C ALLELE; ISOFORM 2).
    2. "Primary structure of ovine alpha s1-caseins: localization of phosphorylation sites and characterization of genetic variants A, C and D."
      Ferranti P., Malorni A., Nitti G., Laezza P., Pizzano R., Chianese L., Addeo F.
      J. Dairy Res. 62:281-296(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 16-214 (A; C AND D ALLELES; ISOFORMS 1 AND 2), PHOSPHORYLATION AT SER-27; SER-56; SER-61; SER-63; SER-79; SER-80; SER-81; SER-82; SER-83; SER-90 AND SER-130, MASS SPECTROMETRY.
      Tissue: Milk.
    3. "Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism."
      Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E., Caroli A.
      J. Dairy Sci. 87:2606-2613(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT THR-209.
      Strain: Comisana, Sarda and Sopravissana.
      Tissue: Blood.

    Entry informationi

    Entry nameiCASA1_SHEEP
    AccessioniPrimary (citable) accession number: P04653
    Secondary accession number(s): Q69B23, Q9TS03, Q9TS48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 75 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3