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P04653 (CASA1_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-S1-casein
Gene names
Name:CSN1S1
OrganismOvis aries (Sheep) [Complete proteome]
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Important role in the capacity of milk to transport calcium phosphate.

Subcellular location

Secreted.

Tissue specificity

Mammary gland specific. Secreted in milk.

Polymorphism

At least four alleles are known. The sequence shown is that of allele A. The frequency of variant Thr-209 is 0.263, 0.122 and 0.191 in Italian breeds Comisana, Sarda and Sopravissana, respectively.

Sequence similarities

Belongs to the alpha-casein family.

Mass spectrometry

Isoform 1: Molecular mass is 23638.14±3.0 Da from positions 16 - 214. Determined by ESI. Allele A, with 11 phosphate groups. Ref.2

Isoform 2: Molecular mass is 22620.0±1.6 Da from positions 16 - 214. Determined by ESI. Allele A, with 11 phosphate groups. Ref.2

Isoform 1: Molecular mass is 23561.8±2.3 Da from positions 16 - 214. Determined by ESI. Allele C, with 10 phosphate groups. Ref.2

Isoform 2: Molecular mass is 22551.8±2.2 Da from positions 16 - 214. Determined by ESI. Allele C, with 10 phosphate groups. Ref.2

Isoform 1: Molecular mass is 23290.2±2.9 Da from positions 16 - 214. Determined by ESI. Allele D, with 6 phosphate groups. Ref.2

Isoform 2: Molecular mass is 22629.0±1.6 Da from positions 16 - 214. Determined by ESI. Allele D, with 6 phosphate groups. Ref.2

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionMilk protein
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P04653-2)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P04653-1)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     156-163: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Ref.2
Chain16 – 214199Alpha-S1-casein
PRO_0000004459

Regions

Region105 – 1117Opioid-like peptide sequence

Amino acid modifications

Modified residue271Phosphoserine; in allele A Ref.2
Modified residue561Phosphoserine; in allele C Ref.2
Modified residue611Phosphoserine Ref.2
Modified residue631Phosphoserine Ref.2
Modified residue791Phosphoserine; in alleles A and C Ref.2
Modified residue801Phosphoserine Ref.2
Modified residue811Phosphoserine; in alleles A and C Ref.2
Modified residue821Phosphoserine Ref.2
Modified residue831Phosphoserine; in alleles A and C Ref.2
Modified residue901Phosphoserine Ref.2
Modified residue1301Phosphoserine Ref.2

Natural variations

Alternative sequence156 – 1638Missing in isoform 2.
VSP_012408
Natural variant281S → P in allele C and allele D.
Natural variant831S → N in allele D.
Natural variant2091I → T. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) [UniParc].

Last modified January 4, 2005. Version 3.
Checksum: 9312FBFFBD6E49DC

FASTA21424,306
        10         20         30         40         50         60 
MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE NINELSKDIG 

        70         80         90        100        110        120 
SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED VPSERYLGYL EQLLRLKKYN 

       130        140        150        160        170        180 
VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM IAVNQELAYF YPQLFRQFYQ LDAYPSGAWY 

       190        200        210 
YLPLGTQYTD APSFSDIPNP IGSENSGKIT MPLW 

« Hide

Isoform 2 (Short) [UniParc].

Checksum: E6965F9A79597B9F
Show »

FASTA20623,294

References

[1]"Construction and identification of recombinant plasmids carrying cDNAs coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and beta-lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA."
Mercier J.-C., Gaye P., Soulier S., Hue-Delahaie D., Vilotte J.-L.
Biochimie 67:959-971(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (C ALLELE; ISOFORM 2).
[2]"Primary structure of ovine alpha s1-caseins: localization of phosphorylation sites and characterization of genetic variants A, C and D."
Ferranti P., Malorni A., Nitti G., Laezza P., Pizzano R., Chianese L., Addeo F.
J. Dairy Res. 62:281-296(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 16-214 (A; C AND D ALLELES; ISOFORMS 1 AND 2), PHOSPHORYLATION AT SER-27; SER-56; SER-61; SER-63; SER-79; SER-80; SER-81; SER-82; SER-83; SER-90 AND SER-130, MASS SPECTROMETRY.
Tissue: Milk.
[3]"Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism."
Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E., Caroli A.
J. Dairy Sci. 87:2606-2613(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT THR-209.
Strain: Comisana, Sarda and Sopravissana.
Tissue: Blood.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03237 mRNA. Translation: CAA26982.1.
AY444506 Genomic DNA. Translation: AAS17944.1.
PIRKASHS1. A25069.
RefSeqNP_001009795.1. NM_001009795.1.
UniGeneOar.22214.
Oar.22215.
Oar.429.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome1241. Ovi a 8.
2962. Ovi a 9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID443382.

Organism-specific databases

CTD1446.

Phylogenomic databases

HOVERGENHBG005242.

Family and domain databases

InterProIPR026999. Alpha-s1_casein.
IPR001588. Casein.
[Graphical view]
PANTHERPTHR10240. PTHR10240. 1 hit.
PfamPF00363. Casein. 1 hit.
[Graphical view]
PROSITEPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCASA1_SHEEP
AccessionPrimary (citable) accession number: P04653
Secondary accession number(s): Q69B23, Q9TS03, Q9TS48
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 4, 2005
Last modified: February 19, 2014
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families