Reviewed, UniProtKB/Swiss-Prot P04653 (CASA1_SHEEP)

Names and origin

Protein names

Recommended name:
    Alpha-S1-casein

Gene names

Name:

CSN1S1

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important role in the capacity of milk to transport calcium phosphate.
Subcellular location	Secreted.
Tissue specificity	Mammary gland specific. Secreted in milk.
Polymorphism	At least four alleles are known. The sequence shown is that of allele A. The frequency of variant Thr-209 is 0.263, 0.122 and 0.191 in Italian breeds Comisana, Sarda and Sopravissana, respectively.
Sequence similarities	Belongs to the alpha-casein family.
Mass spectrometry	Molecular weight is 23638.14±3.0 Da from positions 16 - 214 (P04653-2). Determined by ESI. Allele A, with 11 phosphate groups Molecular weight is 22620.0±1.6 Da from positions 16 - 214 (P04653-1). Determined by ESI. Allele A, with 11 phosphate groups Molecular weight is 23561.8±2.3 Da from positions 16 - 214 (P04653-2). Determined by ESI. Allele C, with 10 phosphate groups Molecular weight is 22551.8±2.2 Da from positions 16 - 214 (P04653-1). Determined by ESI. Allele C, with 10 phosphate groups Molecular weight is 23290.2±2.9 Da from positions 16 - 214 (P04653-2). Determined by ESI. Allele D, with 6 phosphate groups Molecular weight is 22629.0±1.6 Da from positions 16 - 214 (P04653-1). Determined by ESI. Allele D, with 6 phosphate groups

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P04653-2)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: Allele A, with 11 phosphate groups Allele C, with 10 phosphate groups Allele D, with 6 phosphate groups

Isoform 2 (identifier: P04653-1)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     156-163: Missing.

Notes: Allele A, with 11 phosphate groups Allele C, with 10 phosphate groups Allele D, with 6 phosphate groups

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 15	15
	Chain	16 – 214	199	Alpha-S1-casein
Regions
	Region	105 – 111	7	Opioid-like peptide sequence
Amino acid modifications
	Modified residue	27	1	Phosphoserine; in allele A
	Modified residue	56	1	Phosphoserine; in allele C
	Modified residue	61	1	Phosphoserine
	Modified residue	63	1	Phosphoserine
	Modified residue	79	1	Phosphoserine; in alleles A and C
	Modified residue	80	1	Phosphoserine
	Modified residue	81	1	Phosphoserine; in alleles A and C
	Modified residue	82	1	Phosphoserine
	Modified residue	83	1	Phosphoserine; in alleles A and C
	Modified residue	90	1	Phosphoserine
	Modified residue	130	1	Phosphoserine
Natural variations
	Alternative sequence	156 – 163	8	Missing in isoform 2.
	Natural variant	28	1	S → P in allele C and allele D.
	Natural variant	83	1	S → N in allele D.
	Natural variant	209	1	I → T

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified January 4, 2005. Version 3. Checksum: 9312FBFFBD6E49DC	FASTA	214	24,306
	10 20 30 40 50 60 MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE NINELSKDIG 70 80 90 100 110 120 SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED VPSERYLGYL EQLLRLKKYN 130 140 150 160 170 180 VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM IAVNQELAYF YPQLFRQFYQ LDAYPSGAWY 190 200 210 YLPLGTQYTD APSFSDIPNP IGSENSGKIT MPLW
	Isoform 2 (Short) [UniParc]. Checksum: E6965F9A79597B9F		206	23,294
	10 20 30 40 50 60 MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE NINELSKDIG 70 80 90 100 110 120 SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED VPSERYLGYL EQLLRLKKYN 130 140 150 160 170 180 VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM IAVNQLFRQF YQLDAYPSGA WYYLPLGTQY 190 200 TDAPSFSDIP NPIGSENSGK ITMPLW

References

[1]	"Construction and identification of recombinant plasmids carrying cDNAs coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and beta-lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA." Mercier J.-C., Gaye P., Soulier S., Hue-Delahaie D., Vilotte J.-L. Biochimie 67:959-971(1985) [PubMed: 3002501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (C ALLELE; ISOFORM 2).
[2]	"Primary structure of ovine alpha s1-caseins: localization of phosphorylation sites and characterization of genetic variants A, C and D." Ferranti P., Malorni A., Nitti G., Laezza P., Pizzano R., Chianese L., Addeo F. J. Dairy Res. 62:281-296(1995) [PubMed: 7601973] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-214 (A; C AND D ALLELES; ISOFORMS 1 AND 2), PHOSPHORYLATION AT SER-27; SER-56; SER-61; SER-63; SER-79; SER-80; SER-81; SER-82; SER-83; SER-90 AND SER-130, MASS SPECTROMETRY. Tissue: Milk.
[3]	"Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism." Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E., Caroli A. J. Dairy Sci. 87:2606-2613(2004) [PubMed: 15328285] [Abstract] Cited for: VARIANT THR-209. Strain: Comisana, Sarda and Sopravissana. Tissue: Blood.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X03237 mRNA. Translation: CAA26982.1. AY444506 Genomic DNA. Translation: AAS17944.1.
PIR	KASHS1. A25069.
RefSeq	NP_001009795.1.
UniGene	Oar.429
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	443382.
Phylogenomic databases
HOVERGEN	P04653.
Family and domain databases
InterPro	IPR001588. Casein. [Graphical view]
Pfam	PF00363. Casein. 1 hit. [Graphical view]
PROSITE	PS00306. CASEIN_ALPHA_BETA. 1 hit. [Graphical view]
ProDom	P04653. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CASA1_SHEEP

Accession

Primary (citable) accession number: P04653
Secondary accession number(s): Q69B23, Q9TS03, Q9TS48

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 4, 2005
Last modified:	July 22, 2008
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)