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P04653

- CASA1_SHEEP

UniProt

P04653 - CASA1_SHEEP

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Protein
Alpha-S1-casein
Gene
CSN1S1
Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Important role in the capacity of milk to transport calcium phosphate.

GO - Molecular functioni

  1. transporter activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Milk protein

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-S1-casein
Gene namesi
Name:CSN1S1
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
ProteomesiUP000002356: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei1241. Ovi a 8.
2962. Ovi a 9.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 15151 Publication
Add
BLAST
Chaini16 – 214199Alpha-S1-casein
PRO_0000004459Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271Phosphoserine; in allele A1 Publication
Modified residuei56 – 561Phosphoserine; in allele C1 Publication
Modified residuei61 – 611Phosphoserine1 Publication
Modified residuei63 – 631Phosphoserine1 Publication
Modified residuei79 – 791Phosphoserine; in alleles A and C1 Publication
Modified residuei80 – 801Phosphoserine1 Publication
Modified residuei81 – 811Phosphoserine; in alleles A and C1 Publication
Modified residuei82 – 821Phosphoserine1 Publication
Modified residuei83 – 831Phosphoserine; in alleles A and C1 Publication
Modified residuei90 – 901Phosphoserine1 Publication
Modified residuei130 – 1301Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Mammary gland specific. Secreted in milk.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni105 – 1117Opioid-like peptide sequence

Sequence similaritiesi

Belongs to the alpha-casein family.

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG005242.

Family and domain databases

InterProiIPR026999. Alpha-s1_casein.
IPR001588. Casein.
[Graphical view]
PANTHERiPTHR10240. PTHR10240. 1 hit.
PfamiPF00363. Casein. 1 hit.
[Graphical view]
PROSITEiPS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P04653-2) [UniParc]FASTAAdd to Basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE    50
NINELSKDIG SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED 100
VPSERYLGYL EQLLRLKKYN VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM 150
IAVNQELAYF YPQLFRQFYQ LDAYPSGAWY YLPLGTQYTD APSFSDIPNP 200
IGSENSGKIT MPLW 214
Length:214
Mass (Da):24,306
Last modified:January 4, 2005 - v3
Checksum:i9312FBFFBD6E49DC
GO
Isoform 2 (identifier: P04653-1) [UniParc]FASTAAdd to Basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     156-163: Missing.

Show »
Length:206
Mass (Da):23,294
Checksum:iE6965F9A79597B9F
GO

Mass spectrometryi

Isoform 1 : Molecular mass is 23638.14±3.0 Da from positions 16 - 214. Determined by ESI. Allele A, with 11 phosphate groups.1 Publication
Isoform 2 : Molecular mass is 22620.0±1.6 Da from positions 16 - 214. Determined by ESI. Allele A, with 11 phosphate groups.1 Publication
Isoform 1 : Molecular mass is 23561.8±2.3 Da from positions 16 - 214. Determined by ESI. Allele C, with 10 phosphate groups.1 Publication
Isoform 2 : Molecular mass is 22551.8±2.2 Da from positions 16 - 214. Determined by ESI. Allele C, with 10 phosphate groups.1 Publication
Isoform 1 : Molecular mass is 23290.2±2.9 Da from positions 16 - 214. Determined by ESI. Allele D, with 6 phosphate groups.1 Publication
Isoform 2 : Molecular mass is 22629.0±1.6 Da from positions 16 - 214. Determined by ESI. Allele D, with 6 phosphate groups.1 Publication

Polymorphismi

At least four alleles are known. The sequence shown is that of allele A. The frequency of variant Thr-209 is 0.263, 0.122 and 0.191 in Italian breeds Comisana, Sarda and Sopravissana, respectively.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti28 – 281S → P in allele C and allele D.
Natural varianti83 – 831S → N in allele D.
Natural varianti209 – 2091I → T.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei156 – 1638Missing in isoform 2.
VSP_012408

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03237 mRNA. Translation: CAA26982.1.
AY444506 Genomic DNA. Translation: AAS17944.1.
PIRiA25069. KASHS1.
RefSeqiNP_001009795.1. NM_001009795.1.
UniGeneiOar.22214.
Oar.22215.
Oar.429.

Genome annotation databases

GeneIDi443382.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03237 mRNA. Translation: CAA26982.1 .
AY444506 Genomic DNA. Translation: AAS17944.1 .
PIRi A25069. KASHS1.
RefSeqi NP_001009795.1. NM_001009795.1.
UniGenei Oar.22214.
Oar.22215.
Oar.429.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 1241. Ovi a 8.
2962. Ovi a 9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 443382.

Organism-specific databases

CTDi 1446.

Phylogenomic databases

HOVERGENi HBG005242.

Family and domain databases

InterProi IPR026999. Alpha-s1_casein.
IPR001588. Casein.
[Graphical view ]
PANTHERi PTHR10240. PTHR10240. 1 hit.
Pfami PF00363. Casein. 1 hit.
[Graphical view ]
PROSITEi PS00306. CASEIN_ALPHA_BETA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Construction and identification of recombinant plasmids carrying cDNAs coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and beta-lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA."
    Mercier J.-C., Gaye P., Soulier S., Hue-Delahaie D., Vilotte J.-L.
    Biochimie 67:959-971(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (C ALLELE; ISOFORM 2).
  2. "Primary structure of ovine alpha s1-caseins: localization of phosphorylation sites and characterization of genetic variants A, C and D."
    Ferranti P., Malorni A., Nitti G., Laezza P., Pizzano R., Chianese L., Addeo F.
    J. Dairy Res. 62:281-296(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-214 (A; C AND D ALLELES; ISOFORMS 1 AND 2), PHOSPHORYLATION AT SER-27; SER-56; SER-61; SER-63; SER-79; SER-80; SER-81; SER-82; SER-83; SER-90 AND SER-130, MASS SPECTROMETRY.
    Tissue: Milk.
  3. "Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism."
    Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E., Caroli A.
    J. Dairy Sci. 87:2606-2613(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT THR-209.
    Strain: Comisana, Sarda and Sopravissana.
    Tissue: Blood.

Entry informationi

Entry nameiCASA1_SHEEP
AccessioniPrimary (citable) accession number: P04653
Secondary accession number(s): Q69B23, Q9TS03, Q9TS48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 4, 2005
Last modified: July 9, 2014
This is version 74 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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