Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Osteocalcin

Gene

Bglap

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Calcium 3By similarity
Metal bindingi70 – 701Calcium 2By similarity
Metal bindingi73 – 731Calcium 1By similarity
Metal bindingi73 – 731Calcium 2By similarity
Metal bindingi79 – 791Calcium 1By similarity

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • structural constituent of bone Source: RGD

GO - Biological processi

  • aging Source: RGD
  • biomineral tissue development Source: UniProtKB-KW
  • bone development Source: RGD
  • cell aging Source: RGD
  • cellular response to growth factor stimulus Source: RGD
  • cellular response to vitamin D Source: RGD
  • odontogenesis Source: RGD
  • ossification Source: RGD
  • osteoblast development Source: RGD
  • osteoblast differentiation Source: RGD
  • regulation of bone mineralization Source: InterPro
  • response to activity Source: RGD
  • response to drug Source: RGD
  • response to estrogen Source: RGD
  • response to ethanol Source: RGD
  • response to glucocorticoid Source: RGD
  • response to gravity Source: RGD
  • response to hydroxyisoflavone Source: RGD
  • response to inorganic substance Source: RGD
  • response to mechanical stimulus Source: RGD
  • response to nutrient levels Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to testosterone Source: RGD
  • response to vitamin K Source: RGD
  • response to zinc ion Source: RGD
Complete GO annotation...

Keywords - Biological processi

Biomineralization

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-159740. Gamma-carboxylation of protein precursors.
R-RNO-159763. Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus.
R-RNO-159782. Removal of aminoterminal propeptides from gamma-carboxylated proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Osteocalcin
Alternative name(s):
Bone Gla protein
Short name:
BGP
Gamma-carboxyglutamic acid-containing protein
Gene namesi
Name:Bglap
Synonyms:Bglap2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2206. Bglap.

Subcellular locationi

GO - Cellular componenti

  • cell projection Source: RGD
  • cytoplasm Source: RGD
  • dendrite Source: RGD
  • extracellular region Source: MGI
  • extracellular space Source: RGD
  • Golgi apparatus Source: RGD
  • membrane-bounded vesicle Source: RGD
  • perikaryon Source: RGD
  • rough endoplasmic reticulum Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323CuratedAdd
BLAST
Propeptidei24 – 4926CuratedPRO_0000011092Add
BLAST
Chaini50 – 9950OsteocalcinPRO_0000011093Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581Hydroxyproline1 Publication
Modified residuei66 – 6614-carboxyglutamatePROSITE-ProRule annotationBy similarity
Modified residuei70 – 7014-carboxyglutamatePROSITE-ProRule annotationBy similarity
Disulfide bondi72 ↔ 78PROSITE-ProRule annotation
Modified residuei73 – 7314-carboxyglutamatePROSITE-ProRule annotationBy similarity

Post-translational modificationi

Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Gamma-carboxyglutamic acid, Hydroxylation

Proteomic databases

PaxDbiP04640.
PRIDEiP04640.

Expressioni

Gene expression databases

GenevisibleiP04640. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026530.

Structurei

3D structure databases

ProteinModelPortaliP04640.
SMRiP04640. Positions 62-96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 9647GlaPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Gla (gamma-carboxy-glutamate) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J11Z. Eukaryota.
ENOG410ZS6W. LUCA.
GeneTreeiENSGT00410000026290.
HOGENOMiHOG000115820.
InParanoidiP04640.
OMAiNCDELAD.
OrthoDBiEOG7VDXRQ.
PhylomeDBiP04640.
TreeFamiTF330920.

Family and domain databases

InterProiIPR000294. GLA_domain.
IPR002384. Osteocalcin/MGP.
[Graphical view]
PRINTSiPR00002. GLABONE.
SMARTiSM00069. GLA. 1 hit.
[Graphical view]
SUPFAMiSSF57630. SSF57630. 1 hit.
PROSITEiPS00011. GLA_1. 1 hit.
PS50998. GLA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04640-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLSLLTLL ALTAFCLSDL AGAKPSDSES DKAFMSKQEG SKVVNRLRRY
60 70 80 90
LNNGLGAPAP YPDPLEPHRE VCELNPNCDE LADHIGFQDA YKRIYGTTV
Length:99
Mass (Da):10,927
Last modified:August 13, 1987 - v1
Checksum:i7F18F1866D4E4388
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04141 mRNA. Translation: CAA27761.1.
M11777 mRNA. Translation: AAA40816.1.
M23637 Genomic DNA. Translation: AAA41761.1.
M25490 Genomic DNA. Translation: AAA53280.1.
J04500 Genomic DNA. Translation: AAA41764.1.
PIRiA31856. GERT.
RefSeqiNP_038200.1. NM_013414.1.
XP_006232656.1. XM_006232594.2.
UniGeneiRn.9722.

Genome annotation databases

EnsembliENSRNOT00000026530; ENSRNOP00000026530; ENSRNOG00000019607.
GeneIDi25295.
KEGGirno:25295.
UCSCiRGD:2206. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04141 mRNA. Translation: CAA27761.1.
M11777 mRNA. Translation: AAA40816.1.
M23637 Genomic DNA. Translation: AAA41761.1.
M25490 Genomic DNA. Translation: AAA53280.1.
J04500 Genomic DNA. Translation: AAA41764.1.
PIRiA31856. GERT.
RefSeqiNP_038200.1. NM_013414.1.
XP_006232656.1. XM_006232594.2.
UniGeneiRn.9722.

3D structure databases

ProteinModelPortaliP04640.
SMRiP04640. Positions 62-96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026530.

Proteomic databases

PaxDbiP04640.
PRIDEiP04640.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026530; ENSRNOP00000026530; ENSRNOG00000019607.
GeneIDi25295.
KEGGirno:25295.
UCSCiRGD:2206. rat.

Organism-specific databases

CTDi632.
RGDi2206. Bglap.

Phylogenomic databases

eggNOGiENOG410J11Z. Eukaryota.
ENOG410ZS6W. LUCA.
GeneTreeiENSGT00410000026290.
HOGENOMiHOG000115820.
InParanoidiP04640.
OMAiNCDELAD.
OrthoDBiEOG7VDXRQ.
PhylomeDBiP04640.
TreeFamiTF330920.

Enzyme and pathway databases

ReactomeiR-RNO-159740. Gamma-carboxylation of protein precursors.
R-RNO-159763. Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus.
R-RNO-159782. Removal of aminoterminal propeptides from gamma-carboxylated proteins.

Miscellaneous databases

NextBioi606055.
PROiP04640.

Gene expression databases

GenevisibleiP04640. RN.

Family and domain databases

InterProiIPR000294. GLA_domain.
IPR002384. Osteocalcin/MGP.
[Graphical view]
PRINTSiPR00002. GLABONE.
SMARTiSM00069. GLA. 1 hit.
[Graphical view]
SUPFAMiSSF57630. SSF57630. 1 hit.
PROSITEiPS00011. GLA_1. 1 hit.
PS50998. GLA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of the human gene for bone gla protein utilizing mouse and rat cDNA clones."
    Celeste A.J., Buecker J.L., Kriz R., Wang E.A., Wozney J.M.
    EMBO J. 5:1885-1890(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The propeptide of rat bone gamma-carboxyglutamic acid protein shares homology with other vitamin K-dependent protein precursors."
    Pan L.C., Price P.A.
    Proc. Natl. Acad. Sci. U.S.A. 82:6109-6113(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], HYDROXYLATION AT PRO-58.
  3. "Characterization of the rat osteocalcin gene: stimulation of promoter activity by 1,25-dihydroxyvitamin D3."
    Yoon K., Rutledge S.J.C., Buenaga R.F., Rodan G.A.
    Biochemistry 27:8521-8526(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Molecular structure of the rat bone Gla protein gene and identification of putative regulatory elements."
    Theofan G., Haberstroh L.M., Price P.A.
    DNA 8:213-221(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Structure of the rat osteocalcin gene and regulation of vitamin D-dependent expression."
    Lian J., Stewart C., Puchacz E., Mackowiak S., Shalhoub V., Collart D., Zambetti G., Stein G.
    Proc. Natl. Acad. Sci. U.S.A. 86:1143-1147(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiOSTCN_RAT
AccessioniPrimary (citable) accession number: P04640
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: May 11, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.