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Protein

Apolipoprotein A-I

Gene

Apoa1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-1369062. ABC transporters in lipid homeostasis.
R-RNO-174800. Chylomicron-mediated lipid transport.
R-RNO-194223. HDL-mediated lipid transport.
R-RNO-2168880. Scavenging of heme from plasma.
R-RNO-3000471. Scavenging by Class B Receptors.
R-RNO-3000480. Scavenging by Class A Receptors.
R-RNO-975634. Retinoid metabolism and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein A-I
Short name:
Apo-AI
Short name:
ApoA-I
Alternative name(s):
Apolipoprotein A1
Cleaved into the following chain:
Proapolipoprotein A-I
Short name:
ProapoA-I
Gene namesi
Name:Apoa1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2130. Apoa1.

Subcellular locationi

GO - Cellular componenti

  • blood microparticle Source: Ensembl
  • cell surface Source: RGD
  • discoidal high-density lipoprotein particle Source: RGD
  • endocytic vesicle Source: Ensembl
  • extracellular exosome Source: Ensembl
  • extracellular space Source: RGD
  • nucleus Source: RGD
  • spherical high-density lipoprotein particle Source: Ensembl
  • very-low-density lipoprotein particle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 259241Proapolipoprotein A-IPRO_0000425335Add
BLAST
Chaini25 – 259235Apolipoprotein A-IPRO_0000001954Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei109 – 1091Methionine sulfoxideBy similarity
Modified residuei189 – 1891Methionine sulfoxideBy similarity
Modified residuei236 – 2361Methionine sulfoxideBy similarity

Post-translational modificationi

Glycosylated.By similarity
Palmitoylated.By similarity
Phosphorylation sites are present in the extracellular medium.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Oxidation, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP04639.
PRIDEiP04639.

PTM databases

iPTMnetiP04639.

Expressioni

Tissue specificityi

Major protein of plasma HDL, also found in chylomicrons.

Gene expression databases

GenevisibleiP04639. RN.

Interactioni

Subunit structurei

Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi247155. 1 interaction.
DIPiDIP-29911N.
IntActiP04639. 2 interactions.
STRINGi10116.ENSRNOP00000065206.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati67 – 88221Add
BLAST
Repeati89 – 110222Add
BLAST
Repeati111 – 121113; half-lengthAdd
BLAST
Repeati122 – 143224Add
BLAST
Repeati144 – 161185; truncatedAdd
BLAST
Repeati162 – 183226Add
BLAST
Repeati184 – 203207; truncatedAdd
BLAST
Repeati204 – 225228Add
BLAST
Repeati226 – 236119; half-lengthAdd
BLAST
Repeati237 – 2592310Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 25919310 X approximate tandem repeatsAdd
BLAST

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IWKR. Eukaryota.
ENOG410YGQ6. LUCA.
GeneTreeiENSGT00530000063081.
HOVERGENiHBG105708.
InParanoidiP04639.
KOiK08757.
OMAiKDFATVY.
OrthoDBiEOG7TBC3N.
PhylomeDBiP04639.

Family and domain databases

InterProiIPR000074. ApoA_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04639-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAAVLAVAL VFLTGCQAWE FWQQDEPQSQ WDRVKDFATV YVDAVKDSGR
60 70 80 90 100
DYVSQFESST LGKQLNLNLL DNWDTLGSTV GRLQEQLGPV TQEFWANLEK
110 120 130 140 150
ETDWLRNEMN KDLENVKQKM QPHLDEFQEK WNEEVEAYRQ KLEPLGTELH
160 170 180 190 200
KNAKEMQRHL KVVAEEFRDR MRVNADALRA KFGLYSDQMR ENLAQRLTEI
210 220 230 240 250
KNHPTLIEYH TKASDHLKTL GEKAKPALDD LGQGLMPVLE AWKAKIMSMI

DEAKKKLNA
Length:259
Mass (Da):30,062
Last modified:May 1, 2007 - v2
Checksum:i28538891B2E3A6CD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281Missing in AAB58428 (PubMed:9415807).Curated
Sequence conflicti28 – 281Missing in AAB58429 (PubMed:9415807).Curated
Sequence conflicti28 – 281Missing in AAB58430 (PubMed:9415807).Curated
Sequence conflicti93 – 931E → D in AAB58428 (PubMed:9415807).Curated
Sequence conflicti93 – 931E → D in AAB58429 (PubMed:9415807).Curated
Sequence conflicti93 – 931E → D in AAB58430 (PubMed:9415807).Curated
Sequence conflicti105 – 1051L → P in AAB58428 (PubMed:9415807).Curated
Sequence conflicti105 – 1051L → P in AAB58429 (PubMed:9415807).Curated
Sequence conflicti105 – 1051L → P in AAB58430 (PubMed:9415807).Curated
Sequence conflicti146 – 1461G → A in AAB58428 (PubMed:9415807).Curated
Sequence conflicti146 – 1461G → A in AAB58429 (PubMed:9415807).Curated
Sequence conflicti146 – 1461G → A in AAB58430 (PubMed:9415807).Curated
Sequence conflicti201 – 2011K → R in CAA25224 (PubMed:6426956).Curated
Sequence conflicti214 – 2141S → G in CAA25224 (PubMed:6426956).Curated
Sequence conflicti218 – 2181K → R in CAA25224 (PubMed:6426956).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M00001 mRNA. Translation: AAA40749.1.
X00558 mRNA. Translation: CAA25224.1.
J02597 Genomic DNA. Translation: AAA40745.1.
U79576 Genomic DNA. Translation: AAB58428.1.
U79577 Genomic DNA. Translation: AAB58429.1.
U79578 Genomic DNA. Translation: AAB58430.1.
BC089820 mRNA. Translation: AAH89820.1.
PIRiA24700.
RefSeqiNP_036870.1. NM_012738.1.
UniGeneiRn.10308.

Genome annotation databases

EnsembliENSRNOT00000074357; ENSRNOP00000065206; ENSRNOG00000045679.
GeneIDi25081.
KEGGirno:25081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M00001 mRNA. Translation: AAA40749.1.
X00558 mRNA. Translation: CAA25224.1.
J02597 Genomic DNA. Translation: AAA40745.1.
U79576 Genomic DNA. Translation: AAB58428.1.
U79577 Genomic DNA. Translation: AAB58429.1.
U79578 Genomic DNA. Translation: AAB58430.1.
BC089820 mRNA. Translation: AAH89820.1.
PIRiA24700.
RefSeqiNP_036870.1. NM_012738.1.
UniGeneiRn.10308.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247155. 1 interaction.
DIPiDIP-29911N.
IntActiP04639. 2 interactions.
STRINGi10116.ENSRNOP00000065206.

PTM databases

iPTMnetiP04639.

Proteomic databases

PaxDbiP04639.
PRIDEiP04639.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000074357; ENSRNOP00000065206; ENSRNOG00000045679.
GeneIDi25081.
KEGGirno:25081.

Organism-specific databases

CTDi335.
RGDi2130. Apoa1.

Phylogenomic databases

eggNOGiENOG410IWKR. Eukaryota.
ENOG410YGQ6. LUCA.
GeneTreeiENSGT00530000063081.
HOVERGENiHBG105708.
InParanoidiP04639.
KOiK08757.
OMAiKDFATVY.
OrthoDBiEOG7TBC3N.
PhylomeDBiP04639.

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-1369062. ABC transporters in lipid homeostasis.
R-RNO-174800. Chylomicron-mediated lipid transport.
R-RNO-194223. HDL-mediated lipid transport.
R-RNO-2168880. Scavenging of heme from plasma.
R-RNO-3000471. Scavenging by Class B Receptors.
R-RNO-3000480. Scavenging by Class A Receptors.
R-RNO-975634. Retinoid metabolism and transport.

Miscellaneous databases

NextBioi605334.
PROiP04639.

Gene expression databases

GenevisibleiP04639. RN.

Family and domain databases

InterProiIPR000074. ApoA_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and structure analysis of the rat apolipoprotein A-I cDNA."
    Poncin J.E., Martial J.A., Gielen J.E.
    Eur. J. Biochem. 140:493-498(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Linkage, evolution, and expression of the rat apolipoprotein A-I, C-III, and A-IV genes."
    Haddad I.A., Ordovas J.M., Fitzpatrick T., Karathanasis S.K.
    J. Biol. Chem. 261:13268-13277(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Repetitive elements in the third intron of murine apolipoprotein A-I gene."
    Chiang A.-N., Fan K.-C., Shaw G.-C., Yang U.-C.
    Biochem. Mol. Biol. Int. 43:989-996(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SHRSP and Wistar Kyoto.
    Tissue: Spleen.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "The primary translation product of rat intestinal apolipoprotein A-I mRNA is an unusual preproprotein."
    Gordon J.I., Smith D.P., Andy R., Alpers D.H., Schonfeld G., Strauss A.W.
    J. Biol. Chem. 257:971-978(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-45.
    Strain: Sprague-Dawley.
  6. Lubec G., Diao W.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 36-46; 162-168 AND 202-212, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.

Entry informationi

Entry nameiAPOA1_RAT
AccessioniPrimary (citable) accession number: P04639
Secondary accession number(s): O08877, O09054, Q5EBB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: May 1, 2007
Last modified: May 11, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.