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Reviewed, UniProtKB/Swiss-Prot P04636 (MDHM_RAT)

Last modified June 16, 2009. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Malate dehydrogenase, mitochondrial
    EC=1.1.1.37
Gene names
Name: Mdh2
Synonyms: Mor1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer.

Subcellular location

Mitochondrion matrix.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion Ref.3
Chain25 – 338314Malate dehydrogenase, mitochondrial
PRO_0000018631

Regions

Nucleotide binding31 – 377NAD By similarity
Nucleotide binding140 – 1423NAD By similarity

Sites

Active site2001Proton acceptor By similarity
Binding site571NAD By similarity
Binding site1041Substrate By similarity
Binding site1101Substrate By similarity
Binding site1171NAD By similarity
Binding site1421Substrate By similarity
Binding site1761Substrate By similarity
Binding site2511NAD By similarity

Amino acid modifications

Modified residue561Phosphotyrosine By similarity
Modified residue1571N6-acetyllysine By similarity
Modified residue2391N6-acetyllysine By similarity
Modified residue3141N6-acetyllysine By similarity

Experimental info

Sequence conflict2291R → K in CAA27812. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P04636-1 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: 13849CCFB78C87E7

FASTA33835,684
        10         20         30         40         50         60 
MLSALARPVG AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH 

        70         80         90        100        110        120 
TPGVAADLSH IETRANVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI 

       130        140        150        160        170        180 
VATLTAACAQ HCPEAMICII SNPVNSTIPI TAEVFKKHGV YNPNKIFGVT TLDIVRANTF 

       190        200        210        220        230        240 
VAELKGLDPA RVNVPVIGGH AGKTIIPLIS QCTPKVDFPQ DQLATLTGRI QEAGTEVVKA 

       250        260        270        280        290        300 
KAGAGSATLS MAYAGARFVF SLVDAMNGKE GVIECSFVQS KETECTYFST PLLLGKKGLE 

       310        320        330 
KNLGIGKITP FEEKMIAEAI PELKASIKKG EDFVKNMK

« Hide

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References

« Hide 'large scale' references
[1]"Isolation and nucleotide sequence of a cDNA clone encoding rat mitochondrial malate dehydrogenase."
Grant P.M., Tellam J., May V.L., Strauss A.W.
Nucleic Acids Res. 14:6053-6066(1986) [PubMed: 3755817] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pituitary anterior lobe.
[3]"Comparison of the precursor and mature forms of rat heart mitochondrial malate dehydrogenase."
Grant P.M., Roderick S.L., Grant G.A., Banaszak L.J., Strauss A.W.
Biochemistry 26:128-134(1987) [PubMed: 3828294] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-338.
[4]Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 27-52; 53-74; 166-185; 192-203; 216-239; 242-296 AND 308-324, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain, Hippocampus and Spinal cord.

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Cross-references

Sequence databases

X04240 mRNA. Translation: CAA27812.1.
BC063165 mRNA. Translation: AAH63165.1.
IPIIPI00197696.
PIRDERTMM. A25509.
RefSeqNP_112413.2.
UniGeneRn.1011

3D structure databases

HSSPHSSP built from PDB template 1MLD based on UniProtKB P00346.
SMRP04636. Positions 25-337.
ModBaseSearch...

PTM databases

PhosphoSiteP04636.

2-D gel databases

Rat-heart-2DPAGEP04636.

Proteomic databases

PRIDEP04636.

Genome annotation databases

EnsemblENSRNOG00000001440. Rattus norvegicus. [Contig view]
GeneID81829.
KEGGrno:81829.
NMPDRfig|10116.3.peg.8382.

Organism-specific databases

RGD619719. Mor1.

Phylogenomic databases

HOVERGENP04636.
OMAP04636. VRSEETE.

Enzyme and pathway databases

BRENDA1.1.1.37. 248.

Gene expression databases

ArrayExpressP04636.
GermOnlineENSRNOG00000001440. Rattus norvegicus.

Family and domain databases

InterProIPR001557. L-lactate/malate_DH.
IPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_NAD-dep_euk_g_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR11540:SF1. MDH_euk_g_bac. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio615775.

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Entry information

Entry nameMDHM_RAT
AccessionPrimary (citable) accession number: P04636
Secondary accession number(s): Q6GSM4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 9, 2007
Last modified: June 16, 2009
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents