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Protein

Malate dehydrogenase, mitochondrial

Gene

Mdh2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Enzyme regulationi

Enzyme activity is enhanced by acetylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57NADBy similarity1
Binding sitei104SubstratePROSITE-ProRule annotation1
Binding sitei110SubstratePROSITE-ProRule annotation1
Binding sitei117NADBy similarity1
Binding sitei142SubstratePROSITE-ProRule annotation1
Binding sitei176SubstratePROSITE-ProRule annotation1
Active sitei200Proton acceptorBy similarity1
Binding sitei251NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 37NADBy similarity7
Nucleotide bindingi140 – 142NADBy similarity3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: RGD
  • malate dehydrogenase (NADP+) activity Source: RGD
  • malate dehydrogenase activity Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • protein self-association Source: RGD

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • internal protein amino acid acetylation Source: UniProtKB
  • malate metabolic process Source: RGD
  • NADH metabolic process Source: RGD
  • oxaloacetate metabolic process Source: RGD
  • tricarboxylic acid cycle Source: RGD

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

ReactomeiR-RNO-70263 Gluconeogenesis
R-RNO-71403 Citric acid cycle (TCA cycle)

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:Mdh2
Synonyms:Mor1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi619719 Mdh2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176835

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 24Mitochondrion1 PublicationAdd BLAST24
ChainiPRO_000001863125 – 338Malate dehydrogenase, mitochondrialAdd BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33O-linked (GlcNAc) serine1 Publication1
Modified residuei78N6-acetyllysine; alternateBy similarity1
Modified residuei78N6-succinyllysine; alternateBy similarity1
Modified residuei91N6-acetyllysine; alternateBy similarity1
Modified residuei91N6-succinyllysine; alternateBy similarity1
Modified residuei165N6-acetyllysineBy similarity1
Modified residuei185N6-acetyllysine; alternateBy similarity1
Modified residuei185N6-succinyllysine; alternateBy similarity1
Modified residuei203N6-succinyllysineBy similarity1
Modified residuei215N6-acetyllysine; alternateBy similarity1
Modified residuei215N6-succinyllysine; alternateBy similarity1
Modified residuei239N6-acetyllysine; alternateBy similarity1
Modified residuei239N6-malonyllysine; alternateBy similarity1
Modified residuei239N6-succinyllysine; alternateBy similarity1
Modified residuei246PhosphoserineCombined sources1
Modified residuei269N6-succinyllysineBy similarity1
Modified residuei296N6-acetyllysine; alternateBy similarity1
Modified residuei296N6-succinyllysine; alternateBy similarity1
Modified residuei301N6-acetyllysine; alternateBy similarity1
Modified residuei301N6-succinyllysine; alternateBy similarity1
Modified residuei307N6-acetyllysine; alternateBy similarity1
Modified residuei307N6-malonyllysine; alternateBy similarity1
Modified residuei307N6-succinyllysine; alternateBy similarity1
Modified residuei314N6-acetyllysine; alternateBy similarity1
Modified residuei314N6-succinyllysine; alternateBy similarity1
Modified residuei324N6-acetyllysine; alternateBy similarity1
Modified residuei324N6-succinyllysine; alternateBy similarity1
Modified residuei326PhosphoserineBy similarity1
Modified residuei328N6-acetyllysine; alternateBy similarity1
Modified residuei328N6-succinyllysine; alternateBy similarity1
Modified residuei329N6-acetyllysine; alternateBy similarity1
Modified residuei329N6-malonyllysine; alternateBy similarity1
Modified residuei335N6-acetyllysine; alternateBy similarity1
Modified residuei335N6-succinyllysine; alternateBy similarity1

Post-translational modificationi

Acetylation is enhanced after treatment either with trichostin A (TCA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP04636
PRIDEiP04636

2D gel databases

World-2DPAGE0004:P04636

PTM databases

CarbonylDBiP04636
iPTMnetiP04636
PhosphoSitePlusiP04636
SwissPalmiP04636

Expressioni

Tissue specificityi

Expressed in flagella of epididymal sperm.1 Publication

Gene expression databases

BgeeiENSRNOG00000001440
GenevisibleiP04636 RN

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein self-association Source: RGD

Protein-protein interaction databases

BioGridi249687, 1 interactor
IntActiP04636, 2 interactors
STRINGi10116.ENSRNOP00000001958

Structurei

3D structure databases

ProteinModelPortaliP04636
SMRiP04636
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1494 Eukaryota
COG0039 LUCA
GeneTreeiENSGT00390000016686
HOGENOMiHOG000213792
HOVERGENiHBG001662
InParanoidiP04636
KOiK00026
OMAiQCTPKVE
OrthoDBiEOG091G0AVO
PhylomeDBiP04636
TreeFamiTF300834

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSALARPVG AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV
60 70 80 90 100
SRLTLYDIAH TPGVAADLSH IETRANVKGY LGPEQLPDCL KGCDVVVIPA
110 120 130 140 150
GVPRKPGMTR DDLFNTNATI VATLTAACAQ HCPEAMICII SNPVNSTIPI
160 170 180 190 200
TAEVFKKHGV YNPNKIFGVT TLDIVRANTF VAELKGLDPA RVNVPVIGGH
210 220 230 240 250
AGKTIIPLIS QCTPKVDFPQ DQLATLTGRI QEAGTEVVKA KAGAGSATLS
260 270 280 290 300
MAYAGARFVF SLVDAMNGKE GVIECSFVQS KETECTYFST PLLLGKKGLE
310 320 330
KNLGIGKITP FEEKMIAEAI PELKASIKKG EDFVKNMK
Length:338
Mass (Da):35,684
Last modified:January 9, 2007 - v2
Checksum:i13849CCFB78C87E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti229R → K in CAA27812 (PubMed:3755817).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04240 mRNA Translation: CAA27812.1
BC063165 mRNA Translation: AAH63165.1
PIRiA25509 DERTMM
RefSeqiNP_112413.2, NM_031151.2
UniGeneiRn.1011

Genome annotation databases

EnsembliENSRNOT00000001958; ENSRNOP00000001958; ENSRNOG00000001440
GeneIDi81829
KEGGirno:81829
UCSCiRGD:619719 rat

Similar proteinsi

Entry informationi

Entry nameiMDHM_RAT
AccessioniPrimary (citable) accession number: P04636
Secondary accession number(s): Q6GSM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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