Malate dehydrogenase, mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot P04636 (MDHM_RAT)

Last modified November 3, 2009. Version 96. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Malate dehydrogenase, mitochondrial
EC=1.1.1.37

Gene names

Name:	Mdh2
Synonyms:	Mor1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	(S)-malate + NAD⁺ = oxaloacetate + NADH.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion matrix.
Tissue specificity	Expressed in flagella of epididymal sperm. Ref.5
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

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Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	NADH metabolic process Inferred from direct assay. Source: RGD glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from direct assay. Source: RGD oxaloacetate metabolic process Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from direct assay. Source: RGD
Cellular component	mitochondrial matrix Inferred from direct assay. Source: RGD
Molecular function	L-malate dehydrogenase activity Inferred from direct assay. Source: RGD malate dehydrogenase (NADP+) activity Inferred from direct assay. Source: RGD protein self-association Inferred from direct assay. Source: RGD
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 24

Mitochondrion Ref.3

Chain

25 – 338

314

Malate dehydrogenase, mitochondrial

PRO_0000018631

Regions

Nucleotide binding

31 – 37

NAD By similarity

Nucleotide binding

140 – 142

NAD By similarity

Sites

Active site

200

Proton acceptor By similarity

Binding site

NAD By similarity

Binding site

104

Substrate By similarity

Binding site

110

Substrate By similarity

Binding site

117

NAD By similarity

Binding site

142

Substrate By similarity

Binding site

176

Substrate By similarity

Binding site

251

NAD By similarity

Amino acid modifications

Modified residue

Phosphotyrosine By similarity

Modified residue

157

N6-acetyllysine By similarity

Modified residue

165

N6-acetyllysine By similarity

Modified residue

185

N6-acetyllysine By similarity

Modified residue

239

N6-acetyllysine By similarity

Modified residue

301

N6-acetyllysine By similarity

Modified residue

314

N6-acetyllysine By similarity

Modified residue

329

N6-acetyllysine By similarity

Modified residue

335

N6-acetyllysine By similarity

Experimental info

Sequence conflict

229

R → K in CAA27812. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P04636-1 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: 13849CCFB78C87E7
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FASTA

338

35,684

        10         20         30         40         50         60 
MLSALARPVG AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH 

        70         80         90        100        110        120 
TPGVAADLSH IETRANVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI 

       130        140        150        160        170        180 
VATLTAACAQ HCPEAMICII SNPVNSTIPI TAEVFKKHGV YNPNKIFGVT TLDIVRANTF 

       190        200        210        220        230        240 
VAELKGLDPA RVNVPVIGGH AGKTIIPLIS QCTPKVDFPQ DQLATLTGRI QEAGTEVVKA 

       250        260        270        280        290        300 
KAGAGSATLS MAYAGARFVF SLVDAMNGKE GVIECSFVQS KETECTYFST PLLLGKKGLE 

       310        320        330 
KNLGIGKITP FEEKMIAEAI PELKASIKKG EDFVKNMK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and nucleotide sequence of a cDNA clone encoding rat mitochondrial malate dehydrogenase." Grant P.M., Tellam J., May V.L., Strauss A.W. Nucleic Acids Res. 14:6053-6066(1986) [PubMed: 3755817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary anterior lobe.
[3]	"Comparison of the precursor and mature forms of rat heart mitochondrial malate dehydrogenase." Grant P.M., Roderick S.L., Grant G.A., Banaszak L.J., Strauss A.W. Biochemistry 26:128-134(1987) [PubMed: 3828294] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-338.
[4]	Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 27-52; 53-74; 166-185; 192-203; 216-239; 242-296 AND 308-324, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain, Hippocampus and Spinal cord.
[5]	"Identification of novel immunodominant epididymal sperm proteins using combinatorial approach." Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V. Reproduction 138:81-93(2009) [PubMed: 19423663] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X04240 mRNA. Translation: CAA27812.1. BC063165 mRNA. Translation: AAH63165.1.
IPI	IPI00197696.
PIR	DERTMM. A25509.
RefSeq	NP_112413.2.
UniGene	Rn.1011
3D structure databases
HSSP	HSSP built from PDB template 1MLD based on UniProtKB P00346.
SMR	P04636. Positions 25-337.
ModBase	Search...
Protein-protein interaction databases
STRING	P04636.
PTM databases
PhosphoSite	P04636.
2-D gel databases
Rat-heart-2DPAGE	P04636.
Proteomic databases
PRIDE	P04636.
Genome annotation databases
Ensembl	ENSRNOT00000001958; ENSRNOP00000001958; ENSRNOG00000001440; Rattus norvegicus. [Genome view]
GeneID	81829.
KEGG	rno:81829.
NMPDR	fig\|10116.3.peg.8382.
UCSC	NM_031151. rat.
Organism-specific databases
CTD	81829.
RGD	619719. Mor1.
Phylogenomic databases
HOVERGEN	P04636.
OMA	VRSEETE.
Enzyme and pathway databases
BRENDA	1.1.1.37. 248.
Gene expression databases
ArrayExpress	P04636.
Genevestigator	P04636.
GermOnline	ENSRNOG00000001440. Rattus norvegicus.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk/g-bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615775.

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Entry information

Entry name

MDHM_RAT

Accession

Primary (citable) accession number: P04636
Secondary accession number(s): Q6GSM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 9, 2007
Last modified:	November 3, 2009
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2-D gel databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents