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Protein

Mitochondrial brown fat uncoupling protein 1

Gene

Ucp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates to non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance (By similarity). Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs (PubMed:12479871, PubMed:16814247). Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria (By similarity).By similarity2 Publications

Enzyme regulationi

Has no constitutive proton transporter activity and has to be activated by long-chain fatty acids/LCFAs. Inhibited by purine nucleotides. Both purine nucleotides and LCFAs bind the cytosolic side of the transporter and directly compete to activate or inhibit it (PubMed:22952235). Activated by noradrenaline and reactive oxygen species (By similarity).By similarity1 Publication

GO - Molecular functioni

  • cardiolipin binding Source: UniProtKB
  • GDP binding Source: UniProtKB
  • GTP binding Source: UniProtKB
  • long-chain fatty acid binding Source: UniProtKB
  • oxidative phosphorylation uncoupler activity Source: UniProtKB
  • transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  • adaptive thermogenesis Source: UniProtKB
  • cellular response to dehydroepiandrosterone Source: RGD
  • cellular response to fatty acid Source: UniProtKB
  • cellular response to hormone stimulus Source: RGD
  • cellular response to reactive oxygen species Source: UniProtKB
  • generation of precursor metabolites and energy Source: RGD
  • mitochondrial transmembrane transport Source: UniProtKB
  • mitochondrial transport Source: RGD
  • positive regulation of multicellular organismal metabolic process Source: RGD
  • proton transport Source: UniProtKB
  • regulation of reactive oxygen species biosynthetic process Source: UniProtKB
  • response to nutrient levels Source: UniProtKB
  • response to temperature stimulus Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-167826. The fatty acid cycling model.
R-RNO-167827. The proton buffering model.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial brown fat uncoupling protein 1Curated
Short name:
UCP 1Curated
Alternative name(s):
Solute carrier family 25 member 7By similarity
ThermogeninBy similarity
Gene namesi
Name:Ucp1Imported
Synonyms:Slc25a7By similarity, Ucp1 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi3931. Ucp1.

Subcellular locationi

  • Mitochondrion inner membrane By similarity; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 10Mitochondrial intermembrane1 Publication9
Transmembranei11 – 32Helical; Name=1Sequence analysisAdd BLAST22
Topological domaini33 – 73Mitochondrial matrix1 PublicationAdd BLAST41
Transmembranei74 – 96Helical; Name=2Sequence analysisAdd BLAST23
Topological domaini97 – 116Mitochondrial intermembrane1 PublicationAdd BLAST20
Transmembranei117 – 133Helical; Name=3Sequence analysisAdd BLAST17
Topological domaini134 – 178Mitochondrial matrix1 PublicationAdd BLAST45
Transmembranei179 – 195Helical; Name=4Sequence analysisAdd BLAST17
Topological domaini196 – 212Mitochondrial intermembrane1 PublicationAdd BLAST17
Transmembranei213 – 232Helical; Name=5Sequence analysisAdd BLAST20
Topological domaini233 – 266Mitochondrial matrix1 PublicationAdd BLAST34
Transmembranei267 – 289Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini290 – 307Mitochondrial intermembrane1 PublicationAdd BLAST18

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: UniProtKB
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi25C → A: No effect on GTP-binding. Loss of fatty acid-induced proton transport; when associated with V-28 and A-31. 1 Publication1
Mutagenesisi28D → V: No effect on GTP-binding. Decreased fatty acid-induced proton transport. Loss of fatty acid-induced proton transport; when associated with A-25 and A-31. 1 Publication1
Mutagenesisi31T → A: No effect on GTP-binding. No effect on fatty acid-induced proton transport. Loss of fatty acid-induced proton transport; when associated with A-25 and V-28. 1 Publication1
Mutagenesisi146H → L: No effect on GTP-binding. Decreased fatty acid-induced proton transport; when associated with L-148. 1 Publication1
Mutagenesisi148H → L: No effect on GTP-binding. Decreased fatty acid-induced proton transport; when associated with L-146. 1 Publication1
Mutagenesisi153R → L: No effect on GTP-binding. Decreased fatty acid-induced proton transport. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000906622 – 307Mitochondrial brown fat uncoupling protein 1Add BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei254Cysteine sulfenic acid (-SOH)By similarity1

Post-translational modificationi

May undergo ubiquitin-mediated proteasomal degradation.1 Publication
May undergo sulfenylation upon cold exposure. May increase the sensitivity of UCP1 thermogenic function to the activation by noradrenaline probably through structural effects.By similarity

Keywords - PTMi

Oxidation

Proteomic databases

PaxDbiP04633.
PRIDEiP04633.

PTM databases

iPTMnetiP04633.
PhosphoSitePlusiP04633.

Expressioni

Tissue specificityi

Brown adipose tissue.1 Publication

Gene expression databases

BgeeiENSRNOG00000003580.
GenevisibleiP04633. RN.

Interactioni

Subunit structurei

Most probably functions as a monomer (By similarity). Binds one purine nucleotide per monomer (PubMed:12479871, PubMed:22952235). However, has also been suggested to function as a homodimer or a homotetramer (By similarity). Tightly associates with cardiolipin in the mitochondrion inner membrane; may stabilize and regulate its activity (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi246975. 1 interactor.
STRINGi10116.ENSRNOP00000004900.

Structurei

3D structure databases

ProteinModelPortaliP04633.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 102Solcar 1Add BLAST92
Repeati111 – 201Solcar 2Add BLAST91
Repeati210 – 295Solcar 3Add BLAST86

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0753. Eukaryota.
ENOG410XRV1. LUCA.
GeneTreeiENSGT00760000119170.
HOGENOMiHOG000165140.
HOVERGENiHBG009528.
InParanoidiP04633.
KOiK08769.
OMAiQGECQTS.
OrthoDBiEOG091G0BQ7.
PhylomeDBiP04633.
TreeFamiTF323211.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04633-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSSTTSEVQ PTMGVKIFSA GVSACLADII TFPLDTAKVR LQIQGEGQAS
60 70 80 90 100
STIRYKGVLG TITTLAKTEG LPKLYSGLPA GIQRQISFAS LRIGLYDTVQ
110 120 130 140 150
EYFSSGRETP ASLGSKISAG LMTGGVAVFI GQPTEVVKVR MQAQSHLHGI
160 170 180 190 200
KPRYTGTYNA YRVIATTESL STLWKGTTPN LMRNVIINCT ELVTYDLMKG
210 220 230 240 250
ALVNHHILAD DVPCHLLSAL VAGFCTTLLA SPVDVVKTRF INSLPGQYPS
260 270 280 290 300
VPSCAMTMYT KEGPAAFFKG FAPSFLRLGS WNVIMFVCFE QLKKELMKSR

QTVDCTT
Length:307
Mass (Da):33,212
Last modified:January 23, 2007 - v2
Checksum:i0B69CEFD3BE6F543
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11814 mRNA. Translation: AAA19671.1.
X03894 mRNA. Translation: CAA27531.1.
BC088156 mRNA. Translation: AAH88156.1.
X12925 Genomic DNA. Translation: CAA31392.1.
PIRiA26294.
RefSeqiNP_036814.1. NM_012682.2.
UniGeneiRn.10281.

Genome annotation databases

EnsembliENSRNOT00000004900; ENSRNOP00000004900; ENSRNOG00000003580.
GeneIDi24860.
KEGGirno:24860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11814 mRNA. Translation: AAA19671.1.
X03894 mRNA. Translation: CAA27531.1.
BC088156 mRNA. Translation: AAH88156.1.
X12925 Genomic DNA. Translation: CAA31392.1.
PIRiA26294.
RefSeqiNP_036814.1. NM_012682.2.
UniGeneiRn.10281.

3D structure databases

ProteinModelPortaliP04633.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246975. 1 interactor.
STRINGi10116.ENSRNOP00000004900.

PTM databases

iPTMnetiP04633.
PhosphoSitePlusiP04633.

Proteomic databases

PaxDbiP04633.
PRIDEiP04633.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004900; ENSRNOP00000004900; ENSRNOG00000003580.
GeneIDi24860.
KEGGirno:24860.

Organism-specific databases

CTDi7350.
RGDi3931. Ucp1.

Phylogenomic databases

eggNOGiKOG0753. Eukaryota.
ENOG410XRV1. LUCA.
GeneTreeiENSGT00760000119170.
HOGENOMiHOG000165140.
HOVERGENiHBG009528.
InParanoidiP04633.
KOiK08769.
OMAiQGECQTS.
OrthoDBiEOG091G0BQ7.
PhylomeDBiP04633.
TreeFamiTF323211.

Enzyme and pathway databases

ReactomeiR-RNO-167826. The fatty acid cycling model.
R-RNO-167827. The proton buffering model.

Miscellaneous databases

PROiP04633.

Gene expression databases

BgeeiENSRNOG00000003580.
GenevisibleiP04633. RN.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUCP1_RAT
AccessioniPrimary (citable) accession number: P04633
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.