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Protein

Calpain small subunit 1

Gene

CAPNS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi112Calcium 1By similarity1
Metal bindingi114Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi119Calcium 1By similarity1
Metal bindingi137Calcium 4By similarity1
Metal bindingi152Calcium 2By similarity1
Metal bindingi154Calcium 2By similarity1
Metal bindingi156Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi158Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi163Calcium 2By similarity1
Metal bindingi182Calcium 3By similarity1
Metal bindingi184Calcium 3By similarity1
Metal bindingi186Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi188Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi193Calcium 3By similarity1
Metal bindingi225Calcium 4By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi108 – 1191PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi152 – 1632PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi182 – 1933PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: ProtInc
  • calcium ion binding Source: InterPro

GO - Biological processi

  • extracellular matrix disassembly Source: Reactome
  • positive regulation of cell proliferation Source: ProtInc
  • regulation of macroautophagy Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000126247-MONOMER.
BRENDAi3.4.22.53. 2681.
3.4.22.B24. 2681.
ReactomeiR-HSA-1474228. Degradation of the extracellular matrix.
R-HSA-6809371. Formation of the cornified envelope.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain small subunit 1
Short name:
CSS1
Alternative name(s):
Calcium-activated neutral proteinase small subunit
Short name:
CANP small subunit
Calcium-dependent protease small subunit
Short name:
CDPS
Calcium-dependent protease small subunit 1
Calpain regulatory subunit
Gene namesi
Name:CAPNS1
Synonyms:CAPN4, CAPNS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:1481. CAPNS1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the plasma membrane upon calcium binding.By similarity

GO - Cellular componenti

  • cytosol Source: BHF-UCL
  • extracellular exosome Source: UniProtKB
  • membrane Source: BHF-UCL
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi826.
OpenTargetsiENSG00000126247.
PharmGKBiPA26067.

Chemistry databases

ChEMBLiCHEMBL2111357.

Polymorphism and mutation databases

BioMutaiCAPNS1.
DMDMi115612.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000737131 – 268Calpain small subunit 1Add BLAST268

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei6PhosphoserineCombined sources1
Modified residuei179N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP04632.
MaxQBiP04632.
PaxDbiP04632.
PeptideAtlasiP04632.
PRIDEiP04632.
TopDownProteomicsiP04632.

2D gel databases

OGPiP04632.
REPRODUCTION-2DPAGEIPI00025084.
SWISS-2DPAGEP04632.

PTM databases

iPTMnetiP04632.
PhosphoSitePlusiP04632.
SwissPalmiP04632.

Expressioni

Gene expression databases

BgeeiENSG00000126247.
CleanExiHS_CAPNS1.
ExpressionAtlasiP04632. baseline and differential.
GenevisibleiP04632. HS.

Organism-specific databases

HPAiHPA006872.

Interactioni

Subunit structurei

Homodimer or heterodimer of a large (catalytic) and a small (regulatory) subunit. In presence of calcium, the heterodimer dissociates (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AGTRAPQ6RW133EBI-711828,EBI-741181
C8orf37Q96NL82EBI-711828,EBI-11904873
CAPN2P176553EBI-711828,EBI-1028956
RASA1P209363EBI-711828,EBI-1026476

Protein-protein interaction databases

BioGridi107276. 59 interactors.
IntActiP04632. 25 interactors.
MINTiMINT-5000880.
STRINGi9606.ENSP00000246533.

Chemistry databases

BindingDBiP04632.

Structurei

Secondary structure

1268
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi97 – 109Combined sources13
Helixi110 – 112Combined sources3
Helixi117 – 130Combined sources14
Helixi141 – 151Combined sources11
Beta strandi156 – 159Combined sources4
Helixi161 – 181Combined sources21
Beta strandi187 – 190Combined sources4
Turni191 – 193Combined sources3
Helixi194 – 200Combined sources7
Helixi207 – 217Combined sources11
Beta strandi222 – 224Combined sources3
Helixi226 – 246Combined sources21
Turni247 – 249Combined sources3
Beta strandi252 – 256Combined sources5
Helixi258 – 265Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KFUX-ray2.50S85-268[»]
1KFXX-ray3.15S85-268[»]
4PHJX-ray1.60A/B96-268[»]
4PHKX-ray2.05A/B96-268[»]
4PHMX-ray2.03A/B96-268[»]
4WQ2X-ray1.64A/B96-268[»]
4WQ3X-ray1.79A/B96-268[»]
5D69X-ray1.97A/B96-268[»]
ProteinModelPortaliP04632.
SMRiP04632.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04632.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 125EF-hand 1; atypicalPROSITE-ProRule annotationAdd BLAST35
Domaini139 – 172EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini169 – 204EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini205 – 233EF-hand 4PROSITE-ProRule annotationAdd BLAST29
Domaini234 – 268EF-hand 5PROSITE-ProRule annotationAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 66Gly-rich (hydrophobic)Add BLAST66
Compositional biasi10 – 26Poly-GlyAdd BLAST17
Compositional biasi35 – 56Poly-GlyAdd BLAST22
Compositional biasi78 – 83Poly-Pro6

Domaini

The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.By similarity
EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.

Sequence similaritiesi

Contains 5 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
ENOG410YKQK. LUCA.
GeneTreeiENSGT00620000087734.
HOGENOMiHOG000063658.
HOVERGENiHBG004492.
InParanoidiP04632.
KOiK08583.
PhylomeDBiP04632.
TreeFamiTF314682.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR029642. CAPN4.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF38. PTHR10183:SF38. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04632-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLVNSFLKG GGGGGGGGGG LGGGLGNVLG GLISGAGGGG GGGGGGGGGG
60 70 80 90 100
GGGGGGTAMR ILGGVISAIS EAAAQYNPEP PPPRTHYSNI EANESEEVRQ
110 120 130 140 150
FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV
160 170 180 190 200
MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA
210 220 230 240 250
AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG
260
TGQIQVNIQE WLQLTMYS
Length:268
Mass (Da):28,316
Last modified:August 13, 1987 - v1
Checksum:i17B87A8E47A90632
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5N → D in AAH64998 (PubMed:15489334).Curated1
Sequence conflicti27N → G in AAH64998 (PubMed:15489334).Curated1
Sequence conflicti34S → G in AAH64998 (PubMed:15489334).Curated1
Sequence conflicti261 – 268WLQLTMYS → VRTPILGYGCLGGPHPSALH TSSELQSPSSYFASRPWVRA KGLVLLGFPVLTLHPPLPSG CS in AAH11903 (PubMed:15489334).Curated8
Sequence conflicti267Y → F in AAH21933 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021089224M → V.1 PublicationCorresponds to variant rs17878750dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04106 mRNA. Translation: CAA27726.1.
M31511
, M31502, M31503, M31504, M31505, M31506, M31507, M31508, M31509, M31510 Genomic DNA. Translation: AAA35646.1.
AK289606 mRNA. Translation: BAF82295.1.
BT009775 mRNA. Translation: AAP88777.1.
AY789642 Genomic DNA. Translation: AAV40829.1.
AD001527 Genomic DNA. Translation: AAB51183.1.
AC002984 Genomic DNA. Translation: AAB81546.1.
BC000592 mRNA. Translation: AAH00592.1.
BC007779 mRNA. Translation: AAH07779.1.
BC011903 mRNA. Translation: AAH11903.1.
BC017308 mRNA. Translation: AAH17308.1.
BC018931 mRNA. Translation: AAH18931.1.
BC021933 mRNA. Translation: AAH21933.1.
BC023643 mRNA. Translation: AAH23643.1.
BC064998 mRNA. Translation: AAH64998.1.
CCDSiCCDS12489.1.
PIRiA26107. CIHUL.
RefSeqiNP_001003962.1. NM_001003962.2.
NP_001289561.1. NM_001302632.1.
NP_001289562.1. NM_001302633.1.
NP_001740.1. NM_001749.3.
XP_005259352.1. XM_005259295.1.
XP_005259353.1. XM_005259296.1.
UniGeneiHs.515371.

Genome annotation databases

EnsembliENST00000246533; ENSP00000246533; ENSG00000126247.
ENST00000588815; ENSP00000464849; ENSG00000126247.
GeneIDi826.
KEGGihsa:826.
UCSCiuc002odi.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

CaBP

Calpain

Calpains homepage
NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04106 mRNA. Translation: CAA27726.1.
M31511
, M31502, M31503, M31504, M31505, M31506, M31507, M31508, M31509, M31510 Genomic DNA. Translation: AAA35646.1.
AK289606 mRNA. Translation: BAF82295.1.
BT009775 mRNA. Translation: AAP88777.1.
AY789642 Genomic DNA. Translation: AAV40829.1.
AD001527 Genomic DNA. Translation: AAB51183.1.
AC002984 Genomic DNA. Translation: AAB81546.1.
BC000592 mRNA. Translation: AAH00592.1.
BC007779 mRNA. Translation: AAH07779.1.
BC011903 mRNA. Translation: AAH11903.1.
BC017308 mRNA. Translation: AAH17308.1.
BC018931 mRNA. Translation: AAH18931.1.
BC021933 mRNA. Translation: AAH21933.1.
BC023643 mRNA. Translation: AAH23643.1.
BC064998 mRNA. Translation: AAH64998.1.
CCDSiCCDS12489.1.
PIRiA26107. CIHUL.
RefSeqiNP_001003962.1. NM_001003962.2.
NP_001289561.1. NM_001302632.1.
NP_001289562.1. NM_001302633.1.
NP_001740.1. NM_001749.3.
XP_005259352.1. XM_005259295.1.
XP_005259353.1. XM_005259296.1.
UniGeneiHs.515371.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KFUX-ray2.50S85-268[»]
1KFXX-ray3.15S85-268[»]
4PHJX-ray1.60A/B96-268[»]
4PHKX-ray2.05A/B96-268[»]
4PHMX-ray2.03A/B96-268[»]
4WQ2X-ray1.64A/B96-268[»]
4WQ3X-ray1.79A/B96-268[»]
5D69X-ray1.97A/B96-268[»]
ProteinModelPortaliP04632.
SMRiP04632.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107276. 59 interactors.
IntActiP04632. 25 interactors.
MINTiMINT-5000880.
STRINGi9606.ENSP00000246533.

Chemistry databases

BindingDBiP04632.
ChEMBLiCHEMBL2111357.

PTM databases

iPTMnetiP04632.
PhosphoSitePlusiP04632.
SwissPalmiP04632.

Polymorphism and mutation databases

BioMutaiCAPNS1.
DMDMi115612.

2D gel databases

OGPiP04632.
REPRODUCTION-2DPAGEIPI00025084.
SWISS-2DPAGEP04632.

Proteomic databases

EPDiP04632.
MaxQBiP04632.
PaxDbiP04632.
PeptideAtlasiP04632.
PRIDEiP04632.
TopDownProteomicsiP04632.

Protocols and materials databases

DNASUi826.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000246533; ENSP00000246533; ENSG00000126247.
ENST00000588815; ENSP00000464849; ENSG00000126247.
GeneIDi826.
KEGGihsa:826.
UCSCiuc002odi.2. human.

Organism-specific databases

CTDi826.
DisGeNETi826.
GeneCardsiCAPNS1.
HGNCiHGNC:1481. CAPNS1.
HPAiHPA006872.
MIMi114170. gene.
neXtProtiNX_P04632.
OpenTargetsiENSG00000126247.
PharmGKBiPA26067.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0037. Eukaryota.
ENOG410YKQK. LUCA.
GeneTreeiENSGT00620000087734.
HOGENOMiHOG000063658.
HOVERGENiHBG004492.
InParanoidiP04632.
KOiK08583.
PhylomeDBiP04632.
TreeFamiTF314682.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000126247-MONOMER.
BRENDAi3.4.22.53. 2681.
3.4.22.B24. 2681.
ReactomeiR-HSA-1474228. Degradation of the extracellular matrix.
R-HSA-6809371. Formation of the cornified envelope.

Miscellaneous databases

ChiTaRSiCAPNS1. human.
EvolutionaryTraceiP04632.
GeneWikiiCAPNS1.
GenomeRNAii826.
PROiP04632.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000126247.
CleanExiHS_CAPNS1.
ExpressionAtlasiP04632. baseline and differential.
GenevisibleiP04632. HS.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR029642. CAPN4.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10183:SF38. PTHR10183:SF38. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPNS1_HUMAN
AccessioniPrimary (citable) accession number: P04632
Secondary accession number(s): A8K0P1, Q8WTX3, Q96EW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 30, 2016
This is version 190 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.