Protein S100-B - Rattus norvegicus (Rat)

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P04631 (S100B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 132. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein S100-B

Alternative name(s):
S-100 protein beta chain
S-100 protein subunit beta
S100 calcium-binding protein B

Gene names

Name:

S100b

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. Ref.8 Ref.9
Subunit structure	Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer interacts with two molecules of CAPZA1. The S100B dimer interacts with two molecules of STK38. Interacts with AGER. Interacts with ATAD3A; this interaction probably occurs in the cytosol prior to ATAD3A mitochondrial targeting. Interacts with CACYBP in a calcium-dependent manner. Ref.7 Ref.8 Ref.9
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Ref.3
Induction	Up-regulated in periinfarct ventricular myocardium. Ref.8
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	Calcium Metal-binding Zinc
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	astrocyte differentiation Inferred from expression pattern PubMed 19147496. Source: RGD cellular response to hypoxia Inferred from expression pattern Ref.8. Source: RGD learning or memory Inferred from sequence or structural similarity. Source: UniProtKB long-term synaptic potentiation Inferred from expression pattern PubMed 21546003. Source: RGD memory Inferred from electronic annotation. Source: Compara negative regulation of skeletal muscle cell differentiation Inferred from mutant phenotype PubMed 20069545. Source: RGD positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from mutant phenotype PubMed 17639288. Source: RGD positive regulation of cell proliferation Inferred from mutant phenotype PubMed 2229184. Source: RGD regulation of cell shape Inferred from mutant phenotype PubMed 2229184. Source: RGD regulation of neuronal synaptic plasticity Inferred from electronic annotation. Source: Compara response to glucocorticoid stimulus Inferred from expression pattern PubMed 16112204. Source: RGD response to methylmercury Inferred from expression pattern PubMed 21783483. Source: RGD
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB extracellular space Inferred from direct assay PubMed 21098642. Source: RGD neuronal cell body Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara ruffle Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from direct assay PubMed 18949447. Source: RGD protein homodimerization activity Inferred from direct assay PubMed 18949447. Source: RGD tau protein binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 92

Protein S100-B

PRO_0000143969

Regions

Domain

13 – 48

EF-hand 1

Domain

49 – 84

EF-hand 2

Calcium binding

19 – 32

1; low affinity

Calcium binding

62 – 73

2; high affinity

Amino acid modifications

Modified residue

N-acetylserine By similarity

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P04631 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 43815AC212BEC7D0
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FASTA

10,744

        10         20         30         40         50         60 
MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET 

        70         80         90 
LDEDGDGECD FQEFMAFVSM VTTACHEFFE HE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and the complete nucleotide sequence of cDNA to mRNA for S-100 protein of rat brain." Kuwano R., Usui H., Maeda T., Fukui T., Yamanari N., Ohtsuka E., Ikehara M., Takahashi Y. Nucleic Acids Res. 12:7455-7465(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and nucleotide sequences of cDNA and genomic DNA for alpha and beta subunits of S100 protein." Kuwano R., Usui H., Maeda T., Araki K., Kurihara T., Yamakuni T., Ohtsuka E., Ikehara M., Takahashi Y. Taniguchi Symp. Brain Sci. 19:243-255(1987) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structure and expression of rat S-100 beta subunit gene." Maeda T., Usui H., Araki K., Kuwano R., Takahashi Y., Suzuki Y. Brain Res. Mol. Brain Res. 10:193-202(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[5]	"Reduction in S100 protein beta subunit mRNA in C6 rat glioma cells following treatment with anti-microtubular drugs." Dunn R., Landry C., O'Hanlon D., Dunn J., Allore R., Brown I., Marks A. J. Biol. Chem. 262:3562-3566(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-92.
[6]	Lubec G., Afjehi-Sadat L., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 7-21 AND 35-56, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Spinal cord.
[7]	"CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family." Filipek A., Jastrzebska B., Nowotny M., Kuznicki J. J. Biol. Chem. 277:28848-28852(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CACYBP.
[8]	"S100B interaction with the receptor for advanced glycation end products (RAGE): a novel receptor-mediated mechanism for myocyte apoptosis postinfarction." Tsoporis J.N., Izhar S., Leong-Poi H., Desjardins J.F., Huttunen H.J., Parker T.G. Circ. Res. 106:93-101(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH AGER, INDUCTION.
[9]	"The calcium-dependent interaction between S100B and the mitochondrial AAA ATPase ATAD3A and the role of this complex in the cytoplasmic processing of ATAD3A." Gilquin B., Cannon B.R., Hubstenberger A., Moulouel B., Falk E., Merle N., Assard N., Kieffer S., Rousseau D., Wilder P.T., Weber D.J., Baudier J. Mol. Cell. Biol. 30:2724-2736(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATAD3A.
[10]	"Solution structure of rat apo-S100B(beta beta) as determined by NMR spectroscopy." Drohat A.C., Amburgey J.C., Abildgaard F., Starich M.R., Baldisseri D.M., Weber D.J. Biochemistry 35:11577-11588(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[11]	"Solution structure of calcium-bound rat S100B(betabeta) as determined by nuclear magnetic resonance spectroscopy." Drohat A.C., Baldisseri D.M., Rustandi R.R., Weber D.J. Biochemistry 37:2729-2740(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[12]	"The use of dipolar couplings for determining the solution structure of rat apo-S100B." Drohat A.C., Tjandra N., Baldisseri D.M., Weber D.J. Protein Sci. 8:800-809(1999) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[13]	"Solution NMR structure of S100B bound to the high-affinity target peptide TRTK-12." Inman K.G., Yang R., Rustandi R.R., Miller K.E., Baldisseri D.M., Weber D.J. J. Mol. Biol. 324:1003-1014(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR IN COMPLEX WITH CAPZA1 AND CALCIUM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X01090 mRNA. Translation: CAA25567.1.
M54919 mRNA. Translation: AAA42096.1.
S53527 Genomic DNA. No translation available.
S53522 Genomic DNA. No translation available.
BC087026 mRNA. Translation: AAH87026.1.
M15705 mRNA. No translation available.

IPI

IPI00231771.

PIR

A26557. A60046.

RefSeq

NP_037323.1. NM_013191.1.

UniGene

Rn.8937.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B4C	NMR	-	A/B	1-92	[»]
1DT7	NMR	-	A/B	1-92	[»]
1MWN	NMR	-	A/B	1-92	[»]
1QLK	NMR	-	A/B	1-92	[»]
1SYM	NMR	-	A/B	1-92	[»]
1XYD	NMR	-	A/B	1-92	[»]
2K7O	NMR	-	A/B	2-92	[»]

ProteinModelPortal

P04631.

SMR

P04631. Positions 1-91.

ModBase

Search...

Protein-protein interaction databases

IntAct

P04631. 1 interaction.

STRING

10116.ENSRNOP00000001743.

PTM databases

PhosphoSite

P04631.

Proteomic databases

PaxDb

P04631.

PRIDE

P04631.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000001743; ENSRNOP00000001743; ENSRNOG00000001295.

GeneID

25742.

KEGG

rno:25742.

UCSC

RGD:3615. rat.

Organism-specific databases

CTD

6285.

RGD

3615. S100b.

Phylogenomic databases

eggNOG

NOG41158.

GeneTree

ENSGT00660000095419.

HOGENOM

HOG000246968.

HOVERGEN

HBG001479.

InParanoid

P04631.

OMA

SDGDGEC.

OrthoDB

EOG4BZN47.

Gene expression databases

Genevestigator

P04631.

GermOnline

ENSRNOG00000001295. Rattus norvegicus.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]

Pfam

PF01023. S_100. 1 hit.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P04631.

NextBio

607903.

Entry information

Entry name

S100B_RAT

Accession

Primary (citable) accession number: P04631

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 132 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents