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P04631

- S100B_RAT

UniProt

P04631 - S100B_RAT

Protein

Protein S100-B

Gene

S100b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi19 – 32141; low affinityAdd
    BLAST
    Calcium bindingi62 – 73122; high affinityAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: RGD
    2. protein binding Source: UniProtKB
    3. protein homodimerization activity Source: RGD
    4. RAGE receptor binding Source: RGD
    5. receptor binding Source: RGD
    6. tau protein binding Source: UniProtKB
    7. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. astrocyte differentiation Source: RGD
    2. cellular response to hypoxia Source: RGD
    3. learning or memory Source: UniProtKB
    4. long-term synaptic potentiation Source: RGD
    5. memory Source: Ensembl
    6. negative regulation of skeletal muscle cell differentiation Source: RGD
    7. positive regulation of apoptotic process Source: RGD
    8. positive regulation of cell proliferation Source: RGD
    9. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
    10. positive regulation of synaptic transmission Source: RGD
    11. regulation of cell shape Source: RGD
    12. regulation of neuronal synaptic plasticity Source: Ensembl
    13. response to glucocorticoid Source: RGD
    14. response to methylmercury Source: RGD

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_194364. RIP-mediated NFkB activation via ZBP1.
    REACT_196231. Advanced glycosylation endproduct receptor signaling.
    REACT_199231. Nuclear signaling by ERBB4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein S100-B
    Alternative name(s):
    S-100 protein beta chain
    S-100 protein subunit beta
    S100 calcium-binding protein B
    Gene namesi
    Name:S100b
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 20

    Organism-specific databases

    RGDi3615. S100b.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. extracellular space Source: RGD
    3. neuronal cell body Source: Ensembl
    4. nucleus Source: UniProtKB-SubCell
    5. perinuclear region of cytoplasm Source: Ensembl
    6. ruffle Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 9291Protein S100-BPRO_0000143969Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP04631.
    PRIDEiP04631.

    PTM databases

    PhosphoSiteiP04631.

    Expressioni

    Tissue specificityi

    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.1 Publication

    Inductioni

    Up-regulated in periinfarct ventricular myocardium.1 Publication

    Gene expression databases

    GenevestigatoriP04631.

    Interactioni

    Subunit structurei

    Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with CACYBP in a calcium-dependent manner. Interacts with ATAD3A; this interaction probably occurs in the cytosol prior to ATAD3A mitochondrial targeting. Interacts with S100A6. The S100B dimer interacts with two molecules of CAPZA1. Interacts with AGER. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AgerQ634953EBI-2696631,EBI-6479195

    Protein-protein interaction databases

    BioGridi247770. 1 interaction.
    IntActiP04631. 2 interactions.
    STRINGi10116.ENSRNOP00000001743.

    Structurei

    Secondary structure

    1
    92
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1816
    Beta strandi21 – 233
    Beta strandi25 – 295
    Helixi30 – 4011
    Helixi41 – 433
    Helixi44 – 474
    Helixi51 – 6212
    Beta strandi66 – 694
    Helixi71 – 8414
    Beta strandi85 – 873
    Turni88 – 903

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B4CNMR-A/B1-92[»]
    1DT7NMR-A/B1-92[»]
    1MWNNMR-A/B1-92[»]
    1QLKNMR-A/B1-92[»]
    1SYMNMR-A/B1-92[»]
    1XYDNMR-A/B1-92[»]
    2K7ONMR-A/B2-92[»]
    ProteinModelPortaliP04631.
    SMRiP04631. Positions 1-91.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04631.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 4836EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini49 – 8436EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the S-100 family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG41158.
    GeneTreeiENSGT00740000114986.
    HOGENOMiHOG000246968.
    HOVERGENiHBG001479.
    InParanoidiP04631.
    OMAiGDAECDF.
    OrthoDBiEOG7R833W.
    PhylomeDBiP04631.
    TreeFamiTF332727.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028481. S100-B.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    [Graphical view]
    PANTHERiPTHR11639:SF17. PTHR11639:SF17. 1 hit.
    PfamiPF01023. S_100. 1 hit.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00303. S100_CABP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04631-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE   50
    QEVVDKVMET LDEDGDGECD FQEFMAFVSM VTTACHEFFE HE 92
    Length:92
    Mass (Da):10,744
    Last modified:January 23, 2007 - v2
    Checksum:i43815AC212BEC7D0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01090 mRNA. Translation: CAA25567.1.
    M54919 mRNA. Translation: AAA42096.1.
    S53527 Genomic DNA. No translation available.
    S53522 Genomic DNA. No translation available.
    BC087026 mRNA. Translation: AAH87026.1.
    M15705 mRNA. No translation available.
    PIRiA60046. A26557.
    RefSeqiNP_037323.1. NM_013191.1.
    XP_006256344.1. XM_006256282.1.
    UniGeneiRn.8937.

    Genome annotation databases

    EnsembliENSRNOT00000001743; ENSRNOP00000001743; ENSRNOG00000001295.
    GeneIDi25742.
    KEGGirno:25742.
    UCSCiRGD:3615. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01090 mRNA. Translation: CAA25567.1 .
    M54919 mRNA. Translation: AAA42096.1 .
    S53527 Genomic DNA. No translation available.
    S53522 Genomic DNA. No translation available.
    BC087026 mRNA. Translation: AAH87026.1 .
    M15705 mRNA. No translation available.
    PIRi A60046. A26557.
    RefSeqi NP_037323.1. NM_013191.1.
    XP_006256344.1. XM_006256282.1.
    UniGenei Rn.8937.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B4C NMR - A/B 1-92 [» ]
    1DT7 NMR - A/B 1-92 [» ]
    1MWN NMR - A/B 1-92 [» ]
    1QLK NMR - A/B 1-92 [» ]
    1SYM NMR - A/B 1-92 [» ]
    1XYD NMR - A/B 1-92 [» ]
    2K7O NMR - A/B 2-92 [» ]
    ProteinModelPortali P04631.
    SMRi P04631. Positions 1-91.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247770. 1 interaction.
    IntActi P04631. 2 interactions.
    STRINGi 10116.ENSRNOP00000001743.

    PTM databases

    PhosphoSitei P04631.

    Proteomic databases

    PaxDbi P04631.
    PRIDEi P04631.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000001743 ; ENSRNOP00000001743 ; ENSRNOG00000001295 .
    GeneIDi 25742.
    KEGGi rno:25742.
    UCSCi RGD:3615. rat.

    Organism-specific databases

    CTDi 6285.
    RGDi 3615. S100b.

    Phylogenomic databases

    eggNOGi NOG41158.
    GeneTreei ENSGT00740000114986.
    HOGENOMi HOG000246968.
    HOVERGENi HBG001479.
    InParanoidi P04631.
    OMAi GDAECDF.
    OrthoDBi EOG7R833W.
    PhylomeDBi P04631.
    TreeFami TF332727.

    Enzyme and pathway databases

    Reactomei REACT_194364. RIP-mediated NFkB activation via ZBP1.
    REACT_196231. Advanced glycosylation endproduct receptor signaling.
    REACT_199231. Nuclear signaling by ERBB4.

    Miscellaneous databases

    EvolutionaryTracei P04631.
    NextBioi 607903.
    PROi P04631.

    Gene expression databases

    Genevestigatori P04631.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028481. S100-B.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    [Graphical view ]
    PANTHERi PTHR11639:SF17. PTHR11639:SF17. 1 hit.
    Pfami PF01023. S_100. 1 hit.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00303. S100_CABP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and the complete nucleotide sequence of cDNA to mRNA for S-100 protein of rat brain."
      Kuwano R., Usui H., Maeda T., Fukui T., Yamanari N., Ohtsuka E., Ikehara M., Takahashi Y.
      Nucleic Acids Res. 12:7455-7465(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning and nucleotide sequences of cDNA and genomic DNA for alpha and beta subunits of S100 protein."
      Kuwano R., Usui H., Maeda T., Araki K., Kurihara T., Yamakuni T., Ohtsuka E., Ikehara M., Takahashi Y.
      Taniguchi Symp. Brain Sci. 19:243-255(1987)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.
    5. "Reduction in S100 protein beta subunit mRNA in C6 rat glioma cells following treatment with anti-microtubular drugs."
      Dunn R., Landry C., O'Hanlon D., Dunn J., Allore R., Brown I., Marks A.
      J. Biol. Chem. 262:3562-3566(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-92.
    6. Lubec G., Afjehi-Sadat L., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 7-21 AND 35-56, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain and Spinal cord.
    7. "CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family."
      Filipek A., Jastrzebska B., Nowotny M., Kuznicki J.
      J. Biol. Chem. 277:28848-28852(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CACYBP.
    8. "S100B interaction with the receptor for advanced glycation end products (RAGE): a novel receptor-mediated mechanism for myocyte apoptosis postinfarction."
      Tsoporis J.N., Izhar S., Leong-Poi H., Desjardins J.F., Huttunen H.J., Parker T.G.
      Circ. Res. 106:93-101(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH AGER, INDUCTION.
    9. "The calcium-dependent interaction between S100B and the mitochondrial AAA ATPase ATAD3A and the role of this complex in the cytoplasmic processing of ATAD3A."
      Gilquin B., Cannon B.R., Hubstenberger A., Moulouel B., Falk E., Merle N., Assard N., Kieffer S., Rousseau D., Wilder P.T., Weber D.J., Baudier J.
      Mol. Cell. Biol. 30:2724-2736(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATAD3A.
    10. "Solution structure of rat apo-S100B(beta beta) as determined by NMR spectroscopy."
      Drohat A.C., Amburgey J.C., Abildgaard F., Starich M.R., Baldisseri D.M., Weber D.J.
      Biochemistry 35:11577-11588(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    11. "Solution structure of calcium-bound rat S100B(betabeta) as determined by nuclear magnetic resonance spectroscopy."
      Drohat A.C., Baldisseri D.M., Rustandi R.R., Weber D.J.
      Biochemistry 37:2729-2740(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    12. "The use of dipolar couplings for determining the solution structure of rat apo-S100B."
      Drohat A.C., Tjandra N., Baldisseri D.M., Weber D.J.
      Protein Sci. 8:800-809(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    13. "Solution NMR structure of S100B bound to the high-affinity target peptide TRTK-12."
      Inman K.G., Yang R., Rustandi R.R., Miller K.E., Baldisseri D.M., Weber D.J.
      J. Mol. Biol. 324:1003-1014(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR IN COMPLEX WITH CAPZA1 AND CALCIUM.

    Entry informationi

    Entry nameiS100B_RAT
    AccessioniPrimary (citable) accession number: P04631
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 144 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3