P04628 (WNT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proto-oncogene Wnt-1

Alternative name(s):
Proto-oncogene Int-1 homolog

Gene names

Name:	WNT1
Synonyms:	INT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
Subunit structure	Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3 By similarity. Interacts with WLS By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Post-translational modification	Palmitoylation at Ser-224 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-93. Palmitoylation is necessary for proper trafficking to cell surface. Ref.5
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Disease	Proto-oncogene
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Spemann organizer formation Inferred from sequence or structural similarity. Source: BHF-UCL T cell differentiation in thymus Inferred from Biological aspect of Ancestor. Source: RefGenome Wnt receptor signaling pathway, calcium modulating pathway Inferred from Biological aspect of Ancestor. Source: RefGenome brain segmentation Inferred from Biological aspect of Ancestor. Source: RefGenome branching involved in ureteric bud morphogenesis Inferred from electronic annotation. Source: Compara canonical Wnt receptor signaling pathway involved in negative regulation of apoptotic process Inferred from mutant phenotype PubMed 12154096. Source: BHF-UCL cellular response to peptide hormone stimulus Inferred from electronic annotation. Source: Compara central nervous system morphogenesis Inferred from sequence or structural similarity. Source: BHF-UCL cerebellum formation Inferred from sequence or structural similarity. Source: BHF-UCL diencephalon development Inferred from Biological aspect of Ancestor. Source: RefGenome embryonic axis specification Inferred from sequence or structural similarity. Source: BHF-UCL forebrain anterior/posterior pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome fourth ventricle development Inferred from Biological aspect of Ancestor. Source: RefGenome hematopoietic stem cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome hepatocyte differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome inner ear morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome mesoderm morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome midbrain development Inferred from sequence or structural similarity. Source: BHF-UCL midbrain-hindbrain boundary maturation during brain development Inferred from Biological aspect of Ancestor. Source: RefGenome myoblast fusion Inferred from electronic annotation. Source: Compara negative regulation of DNA damage checkpoint Inferred from direct assay PubMed 16501043. Source: BHF-UCL negative regulation of cell aging Inferred from direct assay PubMed 16501043. Source: BHF-UCL negative regulation of cell-cell adhesion Inferred from mutant phenotype PubMed 17072303. Source: BHF-UCL negative regulation of cell-substrate adhesion Inferred from direct assay PubMed 16501043. Source: BHF-UCL negative regulation of fat cell differentiation Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: Compara negative regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara neuron fate determination Inferred from Biological aspect of Ancestor. Source: RefGenome organ regeneration Inferred from electronic annotation. Source: Compara positive regulation of Notch signaling pathway Inferred from direct assay PubMed 16501043. Source: BHF-UCL positive regulation of dermatome development Inferred from direct assay PubMed 10654605. Source: BHF-UCL positive regulation of fibroblast proliferation Inferred from mutant phenotype PubMed 19734317. Source: BHF-UCL positive regulation of insulin-like growth factor receptor signaling pathway Inferred from direct assay PubMed 12154096. Source: BHF-UCL positive regulation of lamellipodium assembly Inferred from mutant phenotype PubMed 19756656. Source: BHF-UCL positive regulation of protein phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 19847889. Source: UniProtKB response to wounding Inferred from expression pattern PubMed 17982423. Source: UniProtKB signal transduction in response to DNA damage Inferred from direct assay PubMed 16501043. Source: BHF-UCL spinal cord association neuron differentiation Inferred from electronic annotation. Source: Compara ubiquitin-dependent SMAD protein catabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara early endosome Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome late endosome Inferred from Biological aspect of Ancestor. Source: RefGenome membrane raft Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cytokine activity Inferred from sequence or structural similarity. Source: BHF-UCL frizzled-2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome receptor agonist activity Inferred by curator PubMed 19778454. Source: BHF-UCL transcription regulatory region DNA binding Inferred from direct assay PubMed 19847889. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 370	343	Proto-oncogene Wnt-1		PRO_0000041405
Amino acid modifications
	Lipidation	93	1	S-palmitoyl cysteine Ref.5
	Lipidation	224	1	O-palmitoyl serine; by PORCN Ref.5
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	316	1	N-linked (GlcNAc...) Potential
	Glycosylation	346	1	N-linked (GlcNAc...) Potential
	Glycosylation	359	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P04628 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: F7E8111DA12E173F	FASTA	370	40,982
	10 20 30 40 50 60 MGLWALLPGW VSATLLLALA ALPAALAANS SGRWWGIVNV ASSTNLLTDS KSLQLVLEPS 70 80 90 100 110 120 LQLLSRKQRR LIRQNPGILH SVSGGLQSAV RECKWQFRNR RWNCPTAPGP HLFGKIVNRG 130 140 150 160 170 180 CRETAFIFAI TSAGVTHSVA RSCSEGSIES CTCDYRRRGP GGPDWHWGGC SDNIDFGRLF 190 200 210 220 230 240 GREFVDSGEK GRDLRFLMNL HNNEAGRTTV FSEMRQECKC HGMSGSCTVR TCWMRLPTLR 250 260 270 280 290 300 AVGDVLRDRF DGASRVLYGN RGSNRASRAE LLRLEPEDPA HKPPSPHDLV YFEKSPNFCT 310 320 330 340 350 360 YSGRLGTAGT AGRACNSSSP ALDGCELLCC GRGHRTRTQR VTERCNCTFH WCCHVSCRNC 370 THTRVLHECL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences." van Ooyen A., Kwee V., Nusse R. EMBO J. 4:2905-2909(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	"Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling." Doubravska L., Krausova M., Gradl D., Vojtechova M., Tumova L., Lukas J., Valenta T., Pospichalova V., Fafilek B., Plachy J., Sebesta O., Korinek V. Cell. Signal. 23:837-848(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PALMITOYLATION AT CYS-93 AND SER-224.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03072 Genomic DNA. Translation: CAA26874.1. BT019429 mRNA. Translation: AAV38236.1. CH471111 Genomic DNA. Translation: EAW58030.1. BC074798 mRNA. Translation: AAH74798.1. BC074799 mRNA. Translation: AAH74799.1.
IPI	IPI00025074.
PIR	TVHUT1. A24674.
RefSeq	NP_005421.1. NM_005430.3.
UniGene	Hs.248164.
3D structure databases
ProteinModelPortal	P04628.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000293549.
PTM databases
PhosphoSite	P04628.
Polymorphism databases
DMDM	139743.
Proteomic databases
PaxDb	P04628.
PRIDE	P04628.
Protocols and materials databases
DNASU	7471.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000293549; ENSP00000293549; ENSG00000125084.
GeneID	7471.
KEGG	hsa:7471.
UCSC	uc001rsu.3. human.
Organism-specific databases
CTD	7471.
GeneCards	GC12P049372.
HGNC	HGNC:12774. WNT1.
MIM	164820. gene.
neXtProt	NX_P04628.
PharmGKB	PA37376.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG284879.
HOGENOM	HOG000039528.
HOVERGEN	HBG001595.
InParanoid	P04628.
KO	K03209.
OMA	AIKECKW.
OrthoDB	EOG4R23V2.
PhylomeDB	P04628.
Enzyme and pathway databases
Pathway_Interaction_DB	wnt_canonical_pathway. Canonical Wnt signaling pathway. ps1pathway. Presenilin action in Notch and Wnt signaling.
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P04628.
Bgee	P04628.
CleanEx	HS_WNT1.
Genevestigator	P04628.
GermOnline	ENSG00000125084. Homo sapiens.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009139. Wnt1. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF15. PTHR12027:SF15. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01841. WNT1PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1255129.
GenomeRNAi	7471.
NextBio	29264.
SOURCE	Search...

Entry information

Entry name

WNT1_HUMAN

Accession

Primary (citable) accession number: P04628
Secondary accession number(s): Q5U0N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.