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Protein

Serine/threonine-protein kinase A-Raf

Gene

Araf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Zinc 1By similarity
Metal bindingi112 – 1121Zinc 2By similarity
Metal bindingi115 – 1151Zinc 2By similarity
Metal bindingi125 – 1251Zinc 1By similarity
Metal bindingi128 – 1281Zinc 1By similarity
Metal bindingi133 – 1331Zinc 2By similarity
Metal bindingi136 – 1361Zinc 2By similarity
Metal bindingi144 – 1441Zinc 1By similarity
Binding sitei334 – 3341ATPPROSITE-ProRule annotation
Active sitei427 – 4271Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri98 – 14447Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi314 – 3229ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase A-Raf (EC:2.7.11.1)
Alternative name(s):
Proto-oncogene A-Raf
Gene namesi
Name:Araf
Synonyms:A-raf, Araf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:88065. Araf.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 604603Serine/threonine-protein kinase A-RafPRO_0000085623Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811PhosphothreonineBy similarity
Modified residuei186 – 1861PhosphoserineBy similarity
Modified residuei214 – 2141PhosphoserineBy similarity
Modified residuei223 – 2231PhosphothreonineBy similarity
Modified residuei255 – 2551PhosphoserineBy similarity
Modified residuei297 – 2971PhosphoserineBy similarity
Modified residuei316 – 3161PhosphothreonineBy similarity
Modified residuei522 – 5221Omega-N-methylarginineBy similarity
Modified residuei580 – 5801PhosphoserineBy similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiP04627.
PaxDbiP04627.
PRIDEiP04627.

PTM databases

PhosphoSiteiP04627.

Expressioni

Gene expression databases

BgeeiP04627.
CleanExiMM_ARAF.
ExpressionAtlasiP04627. baseline and differential.
GenevisibleiP04627. MM.

Interactioni

Subunit structurei

Interacts with TH1L/NELFD.By similarity

Protein-protein interaction databases

DIPiDIP-1070N.
IntActiP04627. 5 interactions.
MINTiMINT-1582260.
STRINGi10090.ENSMUSP00000001155.

Structurei

3D structure databases

ProteinModelPortaliP04627.
SMRiP04627. Positions 20-91, 96-146, 275-604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 9173RBDPROSITE-ProRule annotationAdd
BLAST
Domaini308 – 568261Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RBD (Ras-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri98 – 14447Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000252972.
HOVERGENiHBG001886.
InParanoidiP04627.
KOiK08845.
OMAiQTVYESL.
OrthoDBiEOG7F5128.
PhylomeDBiP04627.
TreeFamiTF317006.

Family and domain databases

InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. Raf-like_ras-bd.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPPRGPPVS GAEPSRAVGT VKVYLPNKQR TVVTVREGMS VYDSLDKALK
60 70 80 90 100
VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN
110 120 130 140 150
FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN
160 170 180 190 200
RRQFYHSIQD LSGGSRQQEA PSNLSVNELL TPQGPSPFTQ QRDQEHFSFP
210 220 230 240 250
APANPPLQRI RSTSTPNVHM VSTTAPMDSS LMQFTAQSFS TDAAGRGGDG
260 270 280 290 300
APRGSPSPAS VSSGRKSPHS KLPSEQRERK SLADEKKKVK NLGYRDSGYY
310 320 330 340 350
WEVPPSEVQL LKRIGTGSFG TVFRGRWHGD VAVKVLKVAQ PTAEQAQAFK
360 370 380 390 400
NEMQVLRKTR HVNILLFMGF MTRPGFAIIT QWCEGSSLYH HLHVADTRFD
410 420 430 440 450
MVQLIDVARQ TAQGMDYLHA KNIIHRDLKS NNIFLHEGLT VKIGDFGLAT
460 470 480 490 500
VKTRWSGAQP LEQPSGSVLW MAAEVIRMQD PNPYSFQSDV YAYGVVLYEL
510 520 530 540 550
MTGSLPYSHI GSRDQIIFMV GRGYLSPDLS KIFSNCPKAM RRLLTDCLKF
560 570 580 590 600
QREERPLFPQ ILATIELLQR SLPKIERSAS EPSLHRTQAD ELPACLLSAA

RLVP
Length:604
Mass (Da):67,581
Last modified:August 2, 2002 - v2
Checksum:i05F8262F99DDD087
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti169 – 1691E → K in BAA00018 (PubMed:3491291).Curated
Sequence conflicti186 – 1861S → R in BAB23522 (PubMed:16141072).Curated
Sequence conflicti186 – 1861S → R in BAB26674 (PubMed:16141072).Curated
Sequence conflicti326 – 3261R → L in BAA00018 (PubMed:3491291).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL671885 Genomic DNA. Translation: CAM14589.1.
BC004757 mRNA. Translation: AAH04757.1.
AK004741 mRNA. Translation: BAB23522.1.
AK010060 mRNA. Translation: BAB26674.1.
AK020547 mRNA. Translation: BAB32131.3.
D00024 mRNA. Translation: BAA00018.1.
CCDSiCCDS40886.1.
PIRiA25382. TVMSRF.
RefSeqiNP_001153117.1. NM_001159645.1.
NP_033833.1. NM_009703.2.
XP_006527619.1. XM_006527556.2.
XP_006527620.1. XM_006527557.2.
UniGeneiMm.220946.

Genome annotation databases

EnsembliENSMUST00000001155; ENSMUSP00000001155; ENSMUSG00000001127.
GeneIDi11836.
KEGGimmu:11836.
UCSCiuc009stt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL671885 Genomic DNA. Translation: CAM14589.1.
BC004757 mRNA. Translation: AAH04757.1.
AK004741 mRNA. Translation: BAB23522.1.
AK010060 mRNA. Translation: BAB26674.1.
AK020547 mRNA. Translation: BAB32131.3.
D00024 mRNA. Translation: BAA00018.1.
CCDSiCCDS40886.1.
PIRiA25382. TVMSRF.
RefSeqiNP_001153117.1. NM_001159645.1.
NP_033833.1. NM_009703.2.
XP_006527619.1. XM_006527556.2.
XP_006527620.1. XM_006527557.2.
UniGeneiMm.220946.

3D structure databases

ProteinModelPortaliP04627.
SMRiP04627. Positions 20-91, 96-146, 275-604.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-1070N.
IntActiP04627. 5 interactions.
MINTiMINT-1582260.
STRINGi10090.ENSMUSP00000001155.

PTM databases

PhosphoSiteiP04627.

Proteomic databases

MaxQBiP04627.
PaxDbiP04627.
PRIDEiP04627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001155; ENSMUSP00000001155; ENSMUSG00000001127.
GeneIDi11836.
KEGGimmu:11836.
UCSCiuc009stt.2. mouse.

Organism-specific databases

CTDi369.
MGIiMGI:88065. Araf.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000252972.
HOVERGENiHBG001886.
InParanoidiP04627.
KOiK08845.
OMAiQTVYESL.
OrthoDBiEOG7F5128.
PhylomeDBiP04627.
TreeFamiTF317006.

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.

Miscellaneous databases

ChiTaRSiAraf. mouse.
NextBioi279767.
PROiP04627.
SOURCEiSearch...

Gene expression databases

BgeeiP04627.
CleanExiMM_ARAF.
ExpressionAtlasiP04627. baseline and differential.
GenevisibleiP04627. MM.

Family and domain databases

InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. Raf-like_ras-bd.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-283.
    Strain: C57BL/6J.
    Tissue: Lung, Tongue and Urinary bladder.
  4. "Characterization of murine A-raf, a new oncogene related to the v-raf oncogene."
    Huleihel M., Goldsborough M., Cleveland J., Gunnell M., Bonner T., Rapp U.R.
    Mol. Cell. Biol. 6:2655-2662(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 168-604.

Entry informationi

Entry nameiARAF_MOUSE
AccessioniPrimary (citable) accession number: P04627
Secondary accession number(s): B1AUN9
, Q99J44, Q9CTT5, Q9D6R6, Q9DBU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 2, 2002
Last modified: June 24, 2015
This is version 172 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.