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Protein

Serine/threonine-protein kinase A-Raf

Gene

Araf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Zinc 1By similarity1
Metal bindingi112Zinc 2By similarity1
Metal bindingi115Zinc 2By similarity1
Metal bindingi125Zinc 1By similarity1
Metal bindingi128Zinc 1By similarity1
Metal bindingi133Zinc 2By similarity1
Metal bindingi136Zinc 2By similarity1
Metal bindingi144Zinc 1By similarity1
Binding sitei334ATPPROSITE-ProRule annotation1
Active sitei427Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri98 – 144Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST47
Nucleotide bindingi314 – 322ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiR-MMU-5673000. RAF activation.
R-MMU-5675221. Negative regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase A-Raf (EC:2.7.11.1)
Alternative name(s):
Proto-oncogene A-Raf
Gene namesi
Name:Araf
Synonyms:A-raf, Araf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:88065. Araf.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Ensembl
  • mitochondrion Source: MGI
Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856231 – 604Serine/threonine-protein kinase A-RafAdd BLAST604

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphoserineCombined sources1
Modified residuei162PhosphoserineBy similarity1
Modified residuei181PhosphothreonineCombined sources1
Modified residuei186PhosphoserineBy similarity1
Modified residuei255PhosphoserineBy similarity1
Modified residuei267PhosphoserineBy similarity1
Modified residuei316PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP04627.
MaxQBiP04627.
PaxDbiP04627.
PeptideAtlasiP04627.
PRIDEiP04627.

PTM databases

iPTMnetiP04627.
PhosphoSitePlusiP04627.

Expressioni

Gene expression databases

BgeeiENSMUSG00000001127.
CleanExiMM_ARAF.
ExpressionAtlasiP04627. baseline and differential.
GenevisibleiP04627. MM.

Interactioni

Subunit structurei

Interacts with TH1L/NELFD.By similarity

Protein-protein interaction databases

DIPiDIP-1070N.
IntActiP04627. 6 interactors.
MINTiMINT-1582260.
STRINGi10090.ENSMUSP00000001155.

Structurei

3D structure databases

ProteinModelPortaliP04627.
SMRiP04627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 91RBDPROSITE-ProRule annotationAdd BLAST73
Domaini308 – 568Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RBD (Ras-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri98 – 144Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0193. Eukaryota.
ENOG410Y4UP. LUCA.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000252972.
HOVERGENiHBG001886.
InParanoidiP04627.
KOiK08845.
OMAiLMQLTAQ.
OrthoDBiEOG091G09SB.
PhylomeDBiP04627.
TreeFamiTF317006.

Family and domain databases

CDDicd00029. C1. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. RBD_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPPRGPPVS GAEPSRAVGT VKVYLPNKQR TVVTVREGMS VYDSLDKALK
60 70 80 90 100
VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN
110 120 130 140 150
FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN
160 170 180 190 200
RRQFYHSIQD LSGGSRQQEA PSNLSVNELL TPQGPSPFTQ QRDQEHFSFP
210 220 230 240 250
APANPPLQRI RSTSTPNVHM VSTTAPMDSS LMQFTAQSFS TDAAGRGGDG
260 270 280 290 300
APRGSPSPAS VSSGRKSPHS KLPSEQRERK SLADEKKKVK NLGYRDSGYY
310 320 330 340 350
WEVPPSEVQL LKRIGTGSFG TVFRGRWHGD VAVKVLKVAQ PTAEQAQAFK
360 370 380 390 400
NEMQVLRKTR HVNILLFMGF MTRPGFAIIT QWCEGSSLYH HLHVADTRFD
410 420 430 440 450
MVQLIDVARQ TAQGMDYLHA KNIIHRDLKS NNIFLHEGLT VKIGDFGLAT
460 470 480 490 500
VKTRWSGAQP LEQPSGSVLW MAAEVIRMQD PNPYSFQSDV YAYGVVLYEL
510 520 530 540 550
MTGSLPYSHI GSRDQIIFMV GRGYLSPDLS KIFSNCPKAM RRLLTDCLKF
560 570 580 590 600
QREERPLFPQ ILATIELLQR SLPKIERSAS EPSLHRTQAD ELPACLLSAA

RLVP
Length:604
Mass (Da):67,581
Last modified:August 2, 2002 - v2
Checksum:i05F8262F99DDD087
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti169E → K in BAA00018 (PubMed:3491291).Curated1
Sequence conflicti186S → R in BAB23522 (PubMed:16141072).Curated1
Sequence conflicti186S → R in BAB26674 (PubMed:16141072).Curated1
Sequence conflicti326R → L in BAA00018 (PubMed:3491291).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL671885 Genomic DNA. Translation: CAM14589.1.
BC004757 mRNA. Translation: AAH04757.1.
AK004741 mRNA. Translation: BAB23522.1.
AK010060 mRNA. Translation: BAB26674.1.
AK020547 mRNA. Translation: BAB32131.3.
D00024 mRNA. Translation: BAA00018.1.
CCDSiCCDS40886.1.
PIRiA25382. TVMSRF.
RefSeqiNP_001153117.1. NM_001159645.1.
NP_033833.1. NM_009703.2.
XP_006527619.1. XM_006527556.3.
XP_006527620.1. XM_006527557.3.
UniGeneiMm.220946.

Genome annotation databases

EnsembliENSMUST00000001155; ENSMUSP00000001155; ENSMUSG00000001127.
GeneIDi11836.
KEGGimmu:11836.
UCSCiuc009stt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL671885 Genomic DNA. Translation: CAM14589.1.
BC004757 mRNA. Translation: AAH04757.1.
AK004741 mRNA. Translation: BAB23522.1.
AK010060 mRNA. Translation: BAB26674.1.
AK020547 mRNA. Translation: BAB32131.3.
D00024 mRNA. Translation: BAA00018.1.
CCDSiCCDS40886.1.
PIRiA25382. TVMSRF.
RefSeqiNP_001153117.1. NM_001159645.1.
NP_033833.1. NM_009703.2.
XP_006527619.1. XM_006527556.3.
XP_006527620.1. XM_006527557.3.
UniGeneiMm.220946.

3D structure databases

ProteinModelPortaliP04627.
SMRiP04627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-1070N.
IntActiP04627. 6 interactors.
MINTiMINT-1582260.
STRINGi10090.ENSMUSP00000001155.

PTM databases

iPTMnetiP04627.
PhosphoSitePlusiP04627.

Proteomic databases

EPDiP04627.
MaxQBiP04627.
PaxDbiP04627.
PeptideAtlasiP04627.
PRIDEiP04627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001155; ENSMUSP00000001155; ENSMUSG00000001127.
GeneIDi11836.
KEGGimmu:11836.
UCSCiuc009stt.2. mouse.

Organism-specific databases

CTDi369.
MGIiMGI:88065. Araf.

Phylogenomic databases

eggNOGiKOG0193. Eukaryota.
ENOG410Y4UP. LUCA.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000252972.
HOVERGENiHBG001886.
InParanoidiP04627.
KOiK08845.
OMAiLMQLTAQ.
OrthoDBiEOG091G09SB.
PhylomeDBiP04627.
TreeFamiTF317006.

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiR-MMU-5673000. RAF activation.
R-MMU-5675221. Negative regulation of MAPK pathway.

Miscellaneous databases

ChiTaRSiAraf. mouse.
PROiP04627.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001127.
CleanExiMM_ARAF.
ExpressionAtlasiP04627. baseline and differential.
GenevisibleiP04627. MM.

Family and domain databases

CDDicd00029. C1. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. RBD_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARAF_MOUSE
AccessioniPrimary (citable) accession number: P04627
Secondary accession number(s): B1AUN9
, Q99J44, Q9CTT5, Q9D6R6, Q9DBU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 2, 2002
Last modified: November 30, 2016
This is version 186 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.