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P04627

- ARAF_MOUSE

UniProt

P04627 - ARAF_MOUSE

Protein

Serine/threonine-protein kinase A-Raf

Gene

Araf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 165 (01 Oct 2014)
      Sequence version 2 (02 Aug 2002)
      Previous versions | rss
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    Functioni

    Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi99 – 991Zinc 1By similarity
    Metal bindingi112 – 1121Zinc 2By similarity
    Metal bindingi115 – 1151Zinc 2By similarity
    Metal bindingi125 – 1251Zinc 1By similarity
    Metal bindingi128 – 1281Zinc 1By similarity
    Metal bindingi133 – 1331Zinc 2By similarity
    Metal bindingi136 – 1361Zinc 2By similarity
    Metal bindingi144 – 1441Zinc 1By similarity
    Binding sitei334 – 3341ATPPROSITE-ProRule annotation
    Active sitei427 – 4271Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri98 – 14447Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi314 – 3229ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. protein serine/threonine kinase activity Source: UniProtKB-KW
    5. receptor signaling protein activity Source: InterPro

    GO - Biological processi

    1. negative regulation of apoptotic process Source: Ensembl
    2. positive regulation of peptidyl-serine phosphorylation Source: Ensembl
    3. regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
    4. regulation of TOR signaling Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase A-Raf (EC:2.7.11.1)
    Alternative name(s):
    Proto-oncogene A-Raf
    Gene namesi
    Name:Araf
    Synonyms:A-raf, Araf1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:88065. Araf.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: MGI

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 604604Serine/threonine-protein kinase A-RafPRO_0000085623Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei186 – 1861PhosphoserineBy similarity
    Modified residuei255 – 2551PhosphoserineBy similarity
    Modified residuei316 – 3161PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP04627.
    PaxDbiP04627.
    PRIDEiP04627.

    PTM databases

    PhosphoSiteiP04627.

    Expressioni

    Gene expression databases

    ArrayExpressiP04627.
    BgeeiP04627.
    CleanExiMM_ARAF.
    GenevestigatoriP04627.

    Interactioni

    Subunit structurei

    Interacts with TH1L/NELFD.By similarity

    Protein-protein interaction databases

    DIPiDIP-1070N.
    IntActiP04627. 5 interactions.
    MINTiMINT-1582260.

    Structurei

    3D structure databases

    ProteinModelPortaliP04627.
    SMRiP04627. Positions 20-91, 96-146, 275-604.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 9173RBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini308 – 568261Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RBD (Ras-binding) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri98 – 14447Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117324.
    HOGENOMiHOG000252972.
    HOVERGENiHBG001886.
    InParanoidiB1AUN9.
    KOiK08845.
    OMAiLFHTTRL.
    OrthoDBiEOG7F5128.
    PhylomeDBiP04627.
    TreeFamiTF317006.

    Family and domain databases

    InterProiIPR020454. DAG/PE-bd.
    IPR011009. Kinase-like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR003116. Raf-like_ras-bd.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF02196. RBD. 1 hit.
    [Graphical view]
    PRINTSiPR00008. DAGPEDOMAIN.
    SMARTiSM00109. C1. 1 hit.
    SM00455. RBD. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50898. RBD. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04627-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPPRGPPVS GAEPSRAVGT VKVYLPNKQR TVVTVREGMS VYDSLDKALK    50
    VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN 100
    FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN 150
    RRQFYHSIQD LSGGSRQQEA PSNLSVNELL TPQGPSPFTQ QRDQEHFSFP 200
    APANPPLQRI RSTSTPNVHM VSTTAPMDSS LMQFTAQSFS TDAAGRGGDG 250
    APRGSPSPAS VSSGRKSPHS KLPSEQRERK SLADEKKKVK NLGYRDSGYY 300
    WEVPPSEVQL LKRIGTGSFG TVFRGRWHGD VAVKVLKVAQ PTAEQAQAFK 350
    NEMQVLRKTR HVNILLFMGF MTRPGFAIIT QWCEGSSLYH HLHVADTRFD 400
    MVQLIDVARQ TAQGMDYLHA KNIIHRDLKS NNIFLHEGLT VKIGDFGLAT 450
    VKTRWSGAQP LEQPSGSVLW MAAEVIRMQD PNPYSFQSDV YAYGVVLYEL 500
    MTGSLPYSHI GSRDQIIFMV GRGYLSPDLS KIFSNCPKAM RRLLTDCLKF 550
    QREERPLFPQ ILATIELLQR SLPKIERSAS EPSLHRTQAD ELPACLLSAA 600
    RLVP 604
    Length:604
    Mass (Da):67,581
    Last modified:August 2, 2002 - v2
    Checksum:i05F8262F99DDD087
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti169 – 1691E → K in BAA00018. (PubMed:3491291)Curated
    Sequence conflicti186 – 1861S → R in BAB23522. (PubMed:16141072)Curated
    Sequence conflicti186 – 1861S → R in BAB26674. (PubMed:16141072)Curated
    Sequence conflicti326 – 3261R → L in BAA00018. (PubMed:3491291)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL671885 Genomic DNA. Translation: CAM14589.1.
    BC004757 mRNA. Translation: AAH04757.1.
    AK004741 mRNA. Translation: BAB23522.1.
    AK010060 mRNA. Translation: BAB26674.1.
    AK020547 mRNA. Translation: BAB32131.3.
    D00024 mRNA. Translation: BAA00018.1.
    CCDSiCCDS40886.1.
    PIRiA25382. TVMSRF.
    RefSeqiNP_001153117.1. NM_001159645.1.
    NP_033833.1. NM_009703.2.
    XP_006527619.1. XM_006527556.1.
    XP_006527620.1. XM_006527557.1.
    UniGeneiMm.220946.

    Genome annotation databases

    EnsembliENSMUST00000001155; ENSMUSP00000001155; ENSMUSG00000001127.
    GeneIDi11836.
    KEGGimmu:11836.
    UCSCiuc009stt.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL671885 Genomic DNA. Translation: CAM14589.1 .
    BC004757 mRNA. Translation: AAH04757.1 .
    AK004741 mRNA. Translation: BAB23522.1 .
    AK010060 mRNA. Translation: BAB26674.1 .
    AK020547 mRNA. Translation: BAB32131.3 .
    D00024 mRNA. Translation: BAA00018.1 .
    CCDSi CCDS40886.1.
    PIRi A25382. TVMSRF.
    RefSeqi NP_001153117.1. NM_001159645.1.
    NP_033833.1. NM_009703.2.
    XP_006527619.1. XM_006527556.1.
    XP_006527620.1. XM_006527557.1.
    UniGenei Mm.220946.

    3D structure databases

    ProteinModelPortali P04627.
    SMRi P04627. Positions 20-91, 96-146, 275-604.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-1070N.
    IntActi P04627. 5 interactions.
    MINTi MINT-1582260.

    PTM databases

    PhosphoSitei P04627.

    Proteomic databases

    MaxQBi P04627.
    PaxDbi P04627.
    PRIDEi P04627.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000001155 ; ENSMUSP00000001155 ; ENSMUSG00000001127 .
    GeneIDi 11836.
    KEGGi mmu:11836.
    UCSCi uc009stt.2. mouse.

    Organism-specific databases

    CTDi 369.
    MGIi MGI:88065. Araf.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117324.
    HOGENOMi HOG000252972.
    HOVERGENi HBG001886.
    InParanoidi B1AUN9.
    KOi K08845.
    OMAi LFHTTRL.
    OrthoDBi EOG7F5128.
    PhylomeDBi P04627.
    TreeFami TF317006.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 3474.

    Miscellaneous databases

    ChiTaRSi ARAF. mouse.
    NextBioi 279767.
    PROi P04627.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P04627.
    Bgeei P04627.
    CleanExi MM_ARAF.
    Genevestigatori P04627.

    Family and domain databases

    InterProi IPR020454. DAG/PE-bd.
    IPR011009. Kinase-like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR003116. Raf-like_ras-bd.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF02196. RBD. 1 hit.
    [Graphical view ]
    PRINTSi PR00008. DAGPEDOMAIN.
    SMARTi SM00109. C1. 1 hit.
    SM00455. RBD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50898. RBD. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-283.
      Strain: C57BL/6J.
      Tissue: Lung, Tongue and Urinary bladder.
    4. "Characterization of murine A-raf, a new oncogene related to the v-raf oncogene."
      Huleihel M., Goldsborough M., Cleveland J., Gunnell M., Bonner T., Rapp U.R.
      Mol. Cell. Biol. 6:2655-2662(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 168-604.

    Entry informationi

    Entry nameiARAF_MOUSE
    AccessioniPrimary (citable) accession number: P04627
    Secondary accession number(s): B1AUN9
    , Q99J44, Q9CTT5, Q9D6R6, Q9DBU7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 2, 2002
    Last modified: October 1, 2014
    This is version 165 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3