P04571 (SCP_HEDDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Sarcoplasmic calcium-binding protein Short name=SCP
Organism	Hediste diversicolor (Sandworm) (Nereis diversicolor)
Taxonomic identifier	126592 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Annelida › Polychaeta › Palpata › Aciculata › Phyllodocida › Nereididae › Hediste › Hediste diversicolor species group

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Like parvalbumins, SCP's seem to be more abundant in fast contracting muscles, but no functional relationship can be established from this distribution.
Miscellaneous	The sarcoplasmic calcium-binding proteins are abundant in the muscle of arthropods, mollusks, annelids, and protochordates. This protein has three functional calcium-binding sites; potential site 2 has lost affinity for calcium.
Sequence similarities	Contains 4 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium
Molecular function	Muscle protein
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 174

174

Sarcoplasmic calcium-binding protein

PRO_0000073628

Regions

Domain

3 – 38

36

EF-hand 1

Domain

55 – 90

36

EF-hand 2

Domain

91 – 126

36

EF-hand 3

Domain

127 – 160

34

EF-hand 4

Calcium binding

16 – 27

12

1

Calcium binding

104 – 115

12

2

Calcium binding

138 – 149

12

3

Amino acid modifications

Modified residue

1

1

N-acetylserine

Secondary structure

1

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174

Helix Strand Turn

Details...

Helix

2 – 15

14

Beta strand

20 – 23

4

Helix

25 – 38

14

Helix

45 – 59

15

Helix

61 – 63

3

Turn

64 – 67

4

Helix

72 – 82

11

Helix

86 – 89

4

Helix

90 – 93

4

Helix

95 – 103

9

Beta strand

108 – 112

5

Helix

113 – 122

10

Helix

127 – 129

3

Helix

130 – 137

8

Beta strand

142 – 145

4

Helix

147 – 159

13

Helix

165 – 168

4

Sequences

	Sequence		Length	Mass (Da)
	P04571 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 9569B2FD57AC449B	FASTA	174	19,486
	10 20 30 40 50 60 SDLWVQKMKT YFNRIDFDKD GAITRMDFES MAERFAKESE MKAEHAKVLM DSLTGVWDNF 70 80 90 100 110 120 LTAVAGGKGI DETTFINSMK EMVKNPEAKS VVEGPLPLFF RAVDTNEDNN ISRDEYGIFF 130 140 150 160 170 GMLGLDKTMA PASFDAIDTN NDGLLSLEEF VIAGSDFFMN DGDSTNKVFW GPLV

References

[1]	"Amino acid sequence of a sarcoplasmic calcium-binding protein from the sandworm Nereis diversicolor." Collins J.H., Cox J.A., Theibert J.L. J. Biol. Chem. 263:15378-15385(1988) [PubMed: 3170587] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Three-dimensional structure of a sarcoplasmic calcium-binding protein from Nereis diversicolor." Cook W.J., Ealick S.E., Babu Y.S., Cox J.A., Vijay-Kumar S. J. Biol. Chem. 266:652-656(1991) [PubMed: 1985922] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
[3]	"Structure of a sarcoplasmic calcium-binding protein from Nereis diversicolor refined at 2.0-A resolution." Vijay-Kumar S., Cook W.J. J. Mol. Biol. 224:413-426(1992) [PubMed: 1560459] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[4]	"Nereis sarcoplasmic Ca2+-binding protein has a highly unstructured apo state which is switched to the native state upon binding of the first Ca2+ ion." Precheur B., Cox J.A., Petrova T., Mispelter J., Craescu C.T. FEBS Lett. 395:89-94(1996) [PubMed: 8849695] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

A31989.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Q80	NMR	-	A	1-174	[»]
2SCP	X-ray	2.00	A/B	1-174	[»]

ProteinModelPortal

P04571.

SMR

P04571. Positions 1-174.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca-bd.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

SMART

SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SCP_HEDDI

Accession

Primary (citable) accession number: P04571

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	June 28, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)