P04570 (SCP2_BRALA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Sarcoplasmic calcium-binding proteins II, V, VI, and VII Short name=SCP II Short name=SCP V Short name=SCP VI Short name=SCP VII
Organism	Branchiostoma lanceolatum (Common lancelet) (Amphioxus)
Taxonomic identifier	7740 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Cephalochordata › Branchiostomidae › Branchiostoma

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Like parvalbumins, SCP's seem to be more abundant in fast contracting muscles, but no functional relationship can be established from this distribution.
Miscellaneous	The sarcoplasmic calcium-binding proteins are abundant in the muscle of arthropods, mollusks, annelids, and protochordates. This protein has three functional calcium-binding sites; potential site 4 has lost affinity for calcium. There are 7 different SCP's in amphioxus. The sequence shown here is that of SCP II.
Sequence similarities	Contains 4 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Muscle protein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 185

185

Sarcoplasmic calcium-binding proteins II, V, VI, and VII

PRO_0000073633

Regions

Domain

5 – 41

37

EF-hand 1

Domain

57 – 92

36

EF-hand 2

Domain

102 – 137

36

EF-hand 3

Domain

138 – 173

36

EF-hand 4

Calcium binding

19 – 30

12

1

Calcium binding

70 – 81

12

2

Calcium binding

115 – 126

12

3

Natural variations

Natural variant

25

1

S → A in SCP V.

Natural variant

28

1

D → W in SCP V, VI and VII.

Natural variant

29

1

N → E in SCP VI.

Secondary structure

1

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185

Helix Strand Turn

Details...

Helix

4 – 17

14

Beta strand

23 – 26

4

Helix

28 – 42

15

Helix

43 – 45

3

Helix

48 – 69

22

Helix

79 – 91

13

Helix

96 – 98

3

Helix

103 – 114

12

Beta strand

118 – 120

3

Helix

124 – 130

7

Helix

141 – 149

9

Turn

150 – 153

4

Helix

158 – 170

13

Helix

176 – 181

6

Sequences

	Sequence		Length	Mass (Da)
	P04570 [UniParc]. Last modified November 1, 1990. Version 2. Checksum: 76E3307B55C7595B	FASTA	185	21,286
	10 20 30 40 50 60 GLNDFQKQKI KFTFDFFLDM NHDGSIQDND FEDMMTRYKE VNKGSLSDAD YKSMQASLED 70 80 90 100 110 120 EWRDLKGRAD INKDDVVSWE EYLAMWEKTI ATCKSVADLP AWCQNRIPFL FKGMDVSGDG 130 140 150 160 170 180 IVDLEEFQNY CKNFQLQCAD VPAVYNVITD GGKVTFDLNR YKELYYRLLT SPAADAGNTL MGQKP

References

[1]	"Amino acid sequence of two sarcoplasmic calcium-binding proteins from the protochordate amphioxus." Takagi T., Konishi K., Cox J.A. Biochemistry 25:3585-3592(1986) Cited for: PROTEIN SEQUENCE.
[2]	"Amino acid sequences of four isoforms of amphioxus sarcoplasmic calcium-binding proteins." Takagi T., Cox J.A. Eur. J. Biochem. 192:387-399(1990) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Primary structure of three minor isoforms of amphioxus sarcoplasmic calcium-binding proteins." Takagi T., Valette-Talbi L., Cox J.A. FEBS Lett. 302:159-160(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE (SCP V TO VII).
[4]	"Structure of a sarcoplasmic calcium-binding protein from amphioxus refined at 2.4-A resolution." Cook W.J., Jeffrey L.C., Cox J.A., Vijay-Kumar S. J. Mol. Biol. 229:461-471(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

Cross-references

Sequence databases

PIR

S13183.
S21153.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2SAS	X-ray	2.40	A	1-185	[»]

ProteinModelPortal

P04570.

SMR

P04570. Positions 1-185.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Phylogenomic databases

HOVERGEN

HBG017781.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]

SMART

SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P04570.

Entry information

Entry name

SCP2_BRALA

Accession

Primary (citable) accession number: P04570

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	November 1, 1990
Last modified:	May 1, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program