Reviewed, UniProtKB/Swiss-Prot P04563 (GAST_RAT)

Names and origin

Protein names

Recommended name:
    Gastrin
Cleaved into the following 2 chains:
    1- Recommended name:
            Big gastrin
        Alternative name(s):
            Gastrin-34
              Short name=G34
    2- Recommended name:
            Gastrin

Gene names

Name:	Gast
Synonyms:	Gas

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Subcellular location	Secreted.
Post-translational modification	Sulfation on Tyr-87 enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins and between tissues By similarity.
Sequence similarities	Belongs to the gastrin/cholecystokinin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Phosphoprotein Pyrrolidone carboxylic acid Sulfation
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from direct assay. Source: RGD
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hormone activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Probable
	Propeptide	22 – 58	37			PRO_0000010648
	Peptide	59 – 92	34	Big gastrin		PRO_0000010649
	Peptide	76 – 92	17	Gastrin		PRO_0000010650
	Propeptide	96 – 104	9			PRO_0000010651
Amino acid modifications
	Modified residue	59	1	Pyrrolidone carboxylic acid By similarity
	Modified residue	76	1	Pyrrolidone carboxylic acid By similarity
	Modified residue	87	1	Sulfotyrosine
	Modified residue	92	1	Phenylalanine amide
	Modified residue	96	1	Phosphoserine Ref.3
	Modified residue	103	1	Sulfotyrosine

Sequences

	Sequence		Length	Mass (Da)
	P04563-1 [UniParc]. Last modified November 1, 1990. Version 2. Checksum: 973FD06276BF1E21	FASTA	104	11,832
	10 20 30 40 50 60 MPRLCVCMLV LVLALATFSE ASWKPRSQLQ DASSGPRTNG ALEQHQLEKL GPASHHRRQL 70 80 90 100 GPQGPQHFIA DLSKKQRPPM EEEEEAYGWM DFGRRSAEEE DQYN

References

[1]	"Molecular cloning and sequencing of a rat preprogastrin complementary deoxyribonucleic acid." Fuller P.J., Stone D.L., Brand S.J. Mol. Endocrinol. 1:306-311(1987) [PubMed: 3453895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Gastric antrum.
[2]	"Rat gastrin's amino acid sequence determined from the nucleotide sequence of the mRNA." Schaffer M.H., Agarwal K.L., Noyes B.E. Peptides 3:693-696(1982) [PubMed: 6897117] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 56-92.
[3]	"Processing of the gastrin precursor. Modulation of phosphorylated, sulfated, and amidated products." Varro A., Nemeth J., Bridson J., Lee C., Moore S., Dockray G.J. J. Biol. Chem. 265:21476-21481(1990) [PubMed: 1701434] [Abstract] Cited for: PROTEOLYTIC PROCESSING, PHOSPHORYLATION AT SER-96, SULFATION AT TYR-87 AND TYR-103, AMIDATION AT PHE-92.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M38653 mRNA. Translation: AAA41919.1. M25459 mRNA. Translation: AAA41195.1.
IPI	IPI00196845.
PIR	A40910.
RefSeq	NP_036981.1.
UniGene	Rn.9754
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P04563.
Genome annotation databases
Ensembl	ENSRNOG00000014740. Rattus norvegicus. [Contig view]
GeneID	25320.
KEGG	rno:25320.
Organism-specific databases
RGD	2662. Gast.
Phylogenomic databases
HOVERGEN	P04563.
OMA	P04563. TFSEASW.
Gene expression databases
ArrayExpress	P04563.
GermOnline	ENSRNOG00000014740. Rattus norvegicus.
Family and domain databases
InterPro	IPR001651. Gastrin. IPR013152. Gastrin/cholecystokinin_CS. [Graphical view]
Pfam	PF00918. Gastrin. 1 hit. [Graphical view]
SMART	SM00029. GASTRIN. 1 hit. [Graphical view]
PROSITE	PS00259. GASTRIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	606161.

Entry information

Entry name

GAST_RAT

Accession

Primary (citable) accession number: P04563

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)