Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P04551

- CDK1_SCHPO

UniProt

P04551 - CDK1_SCHPO

Protein

Cyclin-dependent kinase 1

Gene

cdc2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 154 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays a key role in the control of the eukaryotic cell cycle. It is required for entry into S-phase and mitosis. When complexed with cig2, plays a role in G1-S phase transition. When activated and complexed with the cyclin cdc13, it leads to the onset of mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Involved in cell cycle arrest induced by defective RNA splicing. Required for phosphorylation of dis1 to ensure accurate chromosome segregation and for the DNA damage checkpoint.4 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-167 activates it.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei134 – 1341Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein kinase activity Source: PomBase
    3. cyclin-dependent protein serine/threonine kinase activity Source: PomBase
    4. protein binding Source: UniProtKB
    5. protein kinase activity Source: PomBase
    6. protein serine/threonine kinase activity Source: PomBase
    7. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular response to nitrogen starvation Source: PomBase
    2. G2/M transition of mitotic cell cycle Source: PomBase
    3. mitotic DNA replication checkpoint Source: PomBase
    4. mitotic G2 DNA damage checkpoint Source: PomBase
    5. mitotic nuclear division Source: UniProtKB-KW
    6. negative regulation of attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation Source: PomBase
    7. negative regulation of G0 to G1 transition Source: PomBase
    8. negative regulation of induction of conjugation with cellular fusion Source: PomBase
    9. positive regulation of G1/S transition of mitotic cell cycle Source: PomBase
    10. positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
    11. protein phosphorylation Source: PomBase
    12. regulation of cell size Source: PomBase
    13. regulation of meiotic cell cycle Source: PomBase
    14. traversing start control point of mitotic cell cycle Source: PomBase

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 5615.
    ReactomeiREACT_188254. Condensation of Prometaphase Chromosomes.
    REACT_188278. MASTL Facilitates Mitotic Progression.
    REACT_188629. G1/S-Specific Transcription.
    REACT_199398. Cyclin A/B1 associated events during G2/M transition.
    REACT_205526. ERK1 activation.
    REACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_219807. Resolution of Sister Chromatid Cohesion.
    REACT_219991. Cyclin B2 mediated events.
    REACT_221258. Depolymerisation of the Nuclear Lamina.
    REACT_222028. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_222044. Activation of the pre-replicative complex.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDK1
    Alternative name(s):
    Cell division control protein 2
    Cell division protein kinase 1
    p34 protein kinase
    Gene namesi
    Name:cdc2
    Synonyms:cdk1, swo2
    ORF Names:pi002, SPBC11B10.09
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC11B10.09.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cyclin-dependent protein kinase holoenzyme complex Source: PomBase
    2. cytosol Source: PomBase
    3. nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi15 – 151Y → F: Premature entry into mitosis. 1 Publication
    Mutagenesisi67 – 671C → F in cdc2-4W. 1 Publication
    Mutagenesisi67 – 671C → Y in cdc2-3W. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 297297Cyclin-dependent kinase 1PRO_0000085720Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141PhosphothreonineBy similarity
    Modified residuei15 – 151Phosphotyrosine2 Publications
    Modified residuei167 – 1671Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP04551.
    PRIDEiP04551.

    Interactioni

    Subunit structurei

    Forms a stable but non-covalent complex with a regulatory subunit suc1 and with the cyclin cdc13. Also associates with cdc37 and cig2.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    orc2Q091422EBI-1187862,EBI-2028187
    pch1O746272EBI-1187862,EBI-443575
    rum1P403802EBI-1187862,EBI-1187892

    Protein-protein interaction databases

    BioGridi276415. 81 interactions.
    DIPiDIP-1076N.
    IntActiP04551. 6 interactions.
    MINTiMINT-4686713.
    STRINGi4896.SPBC11B10.09-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP04551.
    SMRiP04551. Positions 1-295.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 293290Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    KOiK04563.
    OMAiCHRHRII.
    OrthoDBiEOG7K3TWD.
    PhylomeDBiP04551.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04551-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENYQKVEKI GEGTYGVVYK ARHKLSGRIV AMKKIRLEDE SEGVPSTAIR    50
    EISLLKEVND ENNRSNCVRL LDILHAESKL YLVFEFLDMD LKKYMDRISE 100
    TGATSLDPRL VQKFTYQLVN GVNFCHSRRI IHRDLKPQNL LIDKEGNLKL 150
    ADFGLARSFG VPLRNYTHEI VTLWYRAPEV LLGSRHYSTG VDIWSVGCIF 200
    AEMIRRSPLF PGDSEIDEIF KIFQVLGTPN EEVWPGVTLL QDYKSTFPRW 250
    KRMDLHKVVP NGEEDAIELL SAMLVYDPAH RISAKRALQQ NYLRDFH 297
    Length:297
    Mass (Da):34,359
    Last modified:August 13, 1987 - v1
    Checksum:iD04EF14BA1492875
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12912 Genomic DNA. Translation: AAA35293.1.
    AB004534 Genomic DNA. Translation: BAA21379.1.
    CU329671 Genomic DNA. Translation: CAC37513.1.
    PIRiA23359. TVZP2.
    RefSeqiNP_595629.1. NM_001021523.2.

    Genome annotation databases

    EnsemblFungiiSPBC11B10.09.1; SPBC11B10.09.1:pep; SPBC11B10.09.
    GeneIDi2539869.
    KEGGispo:SPBC11B10.09.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12912 Genomic DNA. Translation: AAA35293.1 .
    AB004534 Genomic DNA. Translation: BAA21379.1 .
    CU329671 Genomic DNA. Translation: CAC37513.1 .
    PIRi A23359. TVZP2.
    RefSeqi NP_595629.1. NM_001021523.2.

    3D structure databases

    ProteinModelPortali P04551.
    SMRi P04551. Positions 1-295.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276415. 81 interactions.
    DIPi DIP-1076N.
    IntActi P04551. 6 interactions.
    MINTi MINT-4686713.
    STRINGi 4896.SPBC11B10.09-1.

    Proteomic databases

    MaxQBi P04551.
    PRIDEi P04551.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC11B10.09.1 ; SPBC11B10.09.1:pep ; SPBC11B10.09 .
    GeneIDi 2539869.
    KEGGi spo:SPBC11B10.09.

    Organism-specific databases

    PomBasei SPBC11B10.09.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    KOi K04563.
    OMAi CHRHRII.
    OrthoDBi EOG7K3TWD.
    PhylomeDBi P04551.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 5615.
    Reactomei REACT_188254. Condensation of Prometaphase Chromosomes.
    REACT_188278. MASTL Facilitates Mitotic Progression.
    REACT_188629. G1/S-Specific Transcription.
    REACT_199398. Cyclin A/B1 associated events during G2/M transition.
    REACT_205526. ERK1 activation.
    REACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_219807. Resolution of Sister Chromatid Cohesion.
    REACT_219991. Cyclin B2 mediated events.
    REACT_221258. Depolymerisation of the Nuclear Lamina.
    REACT_222028. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_222044. Activation of the pre-replicative complex.

    Miscellaneous databases

    NextBioi 20801014.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases."
      Hindley J., Phear G.A.
      Gene 31:129-134(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF CYS-67.
    2. "A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment."
      Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.
      Yeast 16:71-80(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    4. "p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase."
      Brizuela L., Draetta G., Beach D.
      EMBO J. 6:3507-3515(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH SUC1.
    5. "Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis."
      Gould K.L., Nurse P.
      Nature 342:39-45(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-15.
    6. "Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis."
      Bueno A., Russell P.
      Mol. Cell. Biol. 13:2286-2297(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CIG2.
    7. "Mutational analysis of the fission yeast p34cdc2 protein kinase gene."
      McNeill S.A., Nurse P.
      Mol. Gen. Genet. 236:415-426(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF TYR-15.
    8. "Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex."
      Hayles J., Fisher D., Woollard A., Nurse P.
      Cell 78:813-822(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "A quantitative model for the cdc2 control of S phase and mitosis in fission yeast."
      Stern B., Nurse P.
      Trends Genet. 12:345-350(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    10. "Cdc2 tyrosine phosphorylation is required for the DNA damage checkpoint in fission yeast."
      Rhind N., Furnari B., Russell P.
      Genes Dev. 11:504-511(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases."
      Yamano H., Kitamura K., Kominami K., Lehmann A., Hunt T., Toda T.
      Mol. Cell 6:1377-1387(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CIG2.
    12. "Regulation of Cdc2p and Cdc13p is required for cell cycle arrest induced by defective RNA splicing in fission yeast."
      Shimada M., Namikawa-Yamada C., Nakanishi M., Murakami H.
      J. Biol. Chem. 280:32640-32648(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "CDC2 phosphorylation of the fission yeast dis1 ensures accurate chromosome segregation."
      Aoki K., Nakaseko Y., Kinoshita K., Goshima G., Yanagida M.
      Curr. Biol. 16:1627-1635(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Activity of Cdc2 and its interaction with the cyclin Cdc13 depend on the molecular chaperone Cdc37 in Schizosaccharomyces pombe."
      Turnbull E.L., Martin I.V., Fantes P.A.
      J. Cell Sci. 119:292-302(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDC13 AND CDC37.
    15. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    16. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-15 AND THR-167, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiCDK1_SCHPO
    AccessioniPrimary (citable) accession number: P04551
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 154 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3