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Protein

Cyclin-dependent kinase 1

Gene

cdc2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required for entry into S-phase and mitosis. When complexed with cig2, plays a role in G1-S phase transition. When activated and complexed with the cyclin cdc13, it leads to the onset of mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Involved in cell cycle arrest induced by defective RNA splicing. Required for phosphorylation of dis1 to ensure accurate chromosome segregation and for the DNA damage checkpoint.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-167 activates it.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei134Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of conjugation with cellular fusion Source: PomBase
  • negative regulation of double-strand break repair via nonhomologous end joining Source: PomBase
  • negative regulation of exit from mitosis Source: PomBase
  • negative regulation of G0 to G1 transition Source: PomBase
  • negative regulation of mitotic attachment of spindle microtubules to kinetochore Source: PomBase
  • negative regulation of mitotic spindle elongation Source: PomBase
  • negative regulation of mitotic telomere tethering at nuclear periphery Source: PomBase
  • negative regulation of phosphoprotein phosphatase activity Source: PomBase
  • negative regulation of protein dephosphorylation Source: PomBase
  • negative regulation of ubiquitin protein ligase activity Source: PomBase
  • positive regulation of attachment of mitotic spindle microtubules to kinetochore Source: PomBase
  • positive regulation of double-strand break repair via homologous recombination Source: PomBase
  • positive regulation of G1/S transition of mitotic cell cycle Source: PomBase
  • positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • positive regulation of mitotic cell cycle DNA replication Source: PomBase
  • positive regulation of protein localization to nucleus Source: PomBase
  • positive regulation of septation initiation signaling Source: PomBase
  • protein phosphorylation Source: PomBase
  • regulation of meiotic cell cycle Source: PomBase
  • response to intra-S DNA damage checkpoint signaling Source: PomBase
  • response to mitotic DNA integrity checkpoint signaling Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 5613.
ReactomeiR-SPO-68962. Activation of the pre-replicative complex.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SPO-69202. Cyclin E associated events during G1/S transition.
SABIO-RKP04551.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1
Alternative name(s):
Cell division control protein 2
Cell division protein kinase 1
p34 protein kinase
Gene namesi
Name:cdc2
Synonyms:cdk1, swo2
ORF Names:pi002, SPBC11B10.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC11B10.09.
PomBaseiSPBC11B10.09. cdc2.

Subcellular locationi

GO - Cellular componenti

  • cyclin-dependent protein kinase holoenzyme complex Source: PomBase
  • cytosol Source: PomBase
  • mitotic spindle midzone Source: PomBase
  • nuclear chromatin Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15Y → F: Premature entry into mitosis. 1 Publication1
Mutagenesisi67C → F in cdc2-4W. 1 Publication1
Mutagenesisi67C → Y in cdc2-3W. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857201 – 297Cyclin-dependent kinase 1Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphothreonineBy similarity1
Modified residuei15Phosphotyrosine2 Publications1
Modified residuei167Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04551.
PRIDEiP04551.

PTM databases

iPTMnetiP04551.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit suc1 and with the cyclin cdc13. Also associates with cdc37 and cig2.

Binary interactionsi

WithEntry#Exp.IntActNotes
orc2Q091422EBI-1187862,EBI-2028187
pch1O746272EBI-1187862,EBI-443575
rum1P403802EBI-1187862,EBI-1187892

Protein-protein interaction databases

BioGridi276415. 97 interactors.
DIPiDIP-1076N.
IntActiP04551. 6 interactors.
MINTiMINT-4686713.

Structurei

3D structure databases

ProteinModelPortaliP04551.
SMRiP04551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST290

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233024.
InParanoidiP04551.
KOiK04563.
OMAiWIRDESQ.
OrthoDBiEOG092C2FL8.
PhylomeDBiP04551.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENYQKVEKI GEGTYGVVYK ARHKLSGRIV AMKKIRLEDE SEGVPSTAIR
60 70 80 90 100
EISLLKEVND ENNRSNCVRL LDILHAESKL YLVFEFLDMD LKKYMDRISE
110 120 130 140 150
TGATSLDPRL VQKFTYQLVN GVNFCHSRRI IHRDLKPQNL LIDKEGNLKL
160 170 180 190 200
ADFGLARSFG VPLRNYTHEI VTLWYRAPEV LLGSRHYSTG VDIWSVGCIF
210 220 230 240 250
AEMIRRSPLF PGDSEIDEIF KIFQVLGTPN EEVWPGVTLL QDYKSTFPRW
260 270 280 290
KRMDLHKVVP NGEEDAIELL SAMLVYDPAH RISAKRALQQ NYLRDFH
Length:297
Mass (Da):34,359
Last modified:August 13, 1987 - v1
Checksum:iD04EF14BA1492875
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12912 Genomic DNA. Translation: AAA35293.1.
AB004534 Genomic DNA. Translation: BAA21379.1.
CU329671 Genomic DNA. Translation: CAC37513.1.
PIRiA23359. TVZP2.
RefSeqiNP_595629.1. NM_001021523.2.

Genome annotation databases

EnsemblFungiiSPBC11B10.09.1; SPBC11B10.09.1:pep; SPBC11B10.09.
GeneIDi2539869.
KEGGispo:SPBC11B10.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12912 Genomic DNA. Translation: AAA35293.1.
AB004534 Genomic DNA. Translation: BAA21379.1.
CU329671 Genomic DNA. Translation: CAC37513.1.
PIRiA23359. TVZP2.
RefSeqiNP_595629.1. NM_001021523.2.

3D structure databases

ProteinModelPortaliP04551.
SMRiP04551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276415. 97 interactors.
DIPiDIP-1076N.
IntActiP04551. 6 interactors.
MINTiMINT-4686713.

PTM databases

iPTMnetiP04551.

Proteomic databases

MaxQBiP04551.
PRIDEiP04551.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC11B10.09.1; SPBC11B10.09.1:pep; SPBC11B10.09.
GeneIDi2539869.
KEGGispo:SPBC11B10.09.

Organism-specific databases

EuPathDBiFungiDB:SPBC11B10.09.
PomBaseiSPBC11B10.09. cdc2.

Phylogenomic databases

HOGENOMiHOG000233024.
InParanoidiP04551.
KOiK04563.
OMAiWIRDESQ.
OrthoDBiEOG092C2FL8.
PhylomeDBiP04551.

Enzyme and pathway databases

BRENDAi2.7.11.22. 5613.
ReactomeiR-SPO-68962. Activation of the pre-replicative complex.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SPO-69202. Cyclin E associated events during G1/S transition.
SABIO-RKP04551.

Miscellaneous databases

PROiP04551.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDK1_SCHPO
AccessioniPrimary (citable) accession number: P04551
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 2, 2016
This is version 177 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.