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Protein

Cyclin-dependent kinase 1

Gene

cdc2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required for entry into S-phase and mitosis. When complexed with cig2, plays a role in G1-S phase transition. When activated and complexed with the cyclin cdc13, it leads to the onset of mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Involved in cell cycle arrest induced by defective RNA splicing. Required for phosphorylation of dis1 to ensure accurate chromosome segregation and for the DNA damage checkpoint.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-167 activates it.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei134 – 1341Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein kinase activity Source: PomBase
  3. cyclin-dependent protein serine/threonine kinase activity Source: PomBase
  4. protein kinase activity Source: PomBase
  5. protein serine/threonine kinase activity Source: PomBase
  6. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. cellular response to nitrogen starvation Source: PomBase
  3. G2/M transition of mitotic cell cycle Source: PomBase
  4. mitotic nuclear division Source: UniProtKB-KW
  5. negative regulation of attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation Source: PomBase
  6. negative regulation of G0 to G1 transition Source: PomBase
  7. negative regulation of induction of conjugation with cellular fusion Source: PomBase
  8. positive regulation of G1/S transition of mitotic cell cycle Source: PomBase
  9. positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  10. positive regulation of mitotic cytokinesis Source: PomBase
  11. positive regulation of septation initiation signaling Source: PomBase
  12. regulation of cell size Source: PomBase
  13. regulation of meiotic cell cycle Source: PomBase
  14. response to intra-S DNA damage checkpoint signaling Source: PomBase
  15. traversing start control point of mitotic cell cycle Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 5615.
ReactomeiREACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
REACT_222044. Activation of the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1
Alternative name(s):
Cell division control protein 2
Cell division protein kinase 1
p34 protein kinase
Gene namesi
Name:cdc2
Synonyms:cdk1, swo2
ORF Names:pi002, SPBC11B10.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC11B10.09.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cyclin-dependent protein kinase holoenzyme complex Source: PomBase
  2. cytosol Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi15 – 151Y → F: Premature entry into mitosis. 1 Publication
Mutagenesisi67 – 671C → F in cdc2-4W. 1 Publication
Mutagenesisi67 – 671C → Y in cdc2-3W. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Cyclin-dependent kinase 1PRO_0000085720Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphothreonineBy similarity
Modified residuei15 – 151Phosphotyrosine2 Publications
Modified residuei167 – 1671Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04551.
PRIDEiP04551.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit suc1 and with the cyclin cdc13. Also associates with cdc37 and cig2.

Binary interactionsi

WithEntry#Exp.IntActNotes
orc2Q091422EBI-1187862,EBI-2028187
pch1O746272EBI-1187862,EBI-443575
rum1P403802EBI-1187862,EBI-1187892

Protein-protein interaction databases

BioGridi276415. 95 interactions.
DIPiDIP-1076N.
IntActiP04551. 6 interactions.
MINTiMINT-4686713.
STRINGi4896.SPBC11B10.09-1.

Structurei

3D structure databases

ProteinModelPortaliP04551.
SMRiP04551. Positions 1-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 293290Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiP04551.
KOiK04563.
OMAiFAEMVMR.
OrthoDBiEOG7K3TWD.
PhylomeDBiP04551.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENYQKVEKI GEGTYGVVYK ARHKLSGRIV AMKKIRLEDE SEGVPSTAIR
60 70 80 90 100
EISLLKEVND ENNRSNCVRL LDILHAESKL YLVFEFLDMD LKKYMDRISE
110 120 130 140 150
TGATSLDPRL VQKFTYQLVN GVNFCHSRRI IHRDLKPQNL LIDKEGNLKL
160 170 180 190 200
ADFGLARSFG VPLRNYTHEI VTLWYRAPEV LLGSRHYSTG VDIWSVGCIF
210 220 230 240 250
AEMIRRSPLF PGDSEIDEIF KIFQVLGTPN EEVWPGVTLL QDYKSTFPRW
260 270 280 290
KRMDLHKVVP NGEEDAIELL SAMLVYDPAH RISAKRALQQ NYLRDFH
Length:297
Mass (Da):34,359
Last modified:August 13, 1987 - v1
Checksum:iD04EF14BA1492875
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12912 Genomic DNA. Translation: AAA35293.1.
AB004534 Genomic DNA. Translation: BAA21379.1.
CU329671 Genomic DNA. Translation: CAC37513.1.
PIRiA23359. TVZP2.
RefSeqiNP_595629.1. NM_001021523.2.

Genome annotation databases

EnsemblFungiiSPBC11B10.09.1; SPBC11B10.09.1:pep; SPBC11B10.09.
GeneIDi2539869.
KEGGispo:SPBC11B10.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12912 Genomic DNA. Translation: AAA35293.1.
AB004534 Genomic DNA. Translation: BAA21379.1.
CU329671 Genomic DNA. Translation: CAC37513.1.
PIRiA23359. TVZP2.
RefSeqiNP_595629.1. NM_001021523.2.

3D structure databases

ProteinModelPortaliP04551.
SMRiP04551. Positions 1-295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276415. 95 interactions.
DIPiDIP-1076N.
IntActiP04551. 6 interactions.
MINTiMINT-4686713.
STRINGi4896.SPBC11B10.09-1.

Proteomic databases

MaxQBiP04551.
PRIDEiP04551.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC11B10.09.1; SPBC11B10.09.1:pep; SPBC11B10.09.
GeneIDi2539869.
KEGGispo:SPBC11B10.09.

Organism-specific databases

PomBaseiSPBC11B10.09.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiP04551.
KOiK04563.
OMAiFAEMVMR.
OrthoDBiEOG7K3TWD.
PhylomeDBiP04551.

Enzyme and pathway databases

BRENDAi2.7.11.22. 5615.
ReactomeiREACT_215140. CDK-mediated phosphorylation and removal of Cdc6.
REACT_222044. Activation of the pre-replicative complex.

Miscellaneous databases

NextBioi20801014.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases."
    Hindley J., Phear G.A.
    Gene 31:129-134(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF CYS-67.
  2. "A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment."
    Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.
    Yeast 16:71-80(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase."
    Brizuela L., Draetta G., Beach D.
    EMBO J. 6:3507-3515(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSOCIATION WITH SUC1.
  5. "Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis."
    Gould K.L., Nurse P.
    Nature 342:39-45(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-15.
  6. "Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis."
    Bueno A., Russell P.
    Mol. Cell. Biol. 13:2286-2297(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CIG2.
  7. "Mutational analysis of the fission yeast p34cdc2 protein kinase gene."
    McNeill S.A., Nurse P.
    Mol. Gen. Genet. 236:415-426(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF TYR-15.
  8. "Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex."
    Hayles J., Fisher D., Woollard A., Nurse P.
    Cell 78:813-822(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "A quantitative model for the cdc2 control of S phase and mitosis in fission yeast."
    Stern B., Nurse P.
    Trends Genet. 12:345-350(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  10. "Cdc2 tyrosine phosphorylation is required for the DNA damage checkpoint in fission yeast."
    Rhind N., Furnari B., Russell P.
    Genes Dev. 11:504-511(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases."
    Yamano H., Kitamura K., Kominami K., Lehmann A., Hunt T., Toda T.
    Mol. Cell 6:1377-1387(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CIG2.
  12. "Regulation of Cdc2p and Cdc13p is required for cell cycle arrest induced by defective RNA splicing in fission yeast."
    Shimada M., Namikawa-Yamada C., Nakanishi M., Murakami H.
    J. Biol. Chem. 280:32640-32648(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "CDC2 phosphorylation of the fission yeast dis1 ensures accurate chromosome segregation."
    Aoki K., Nakaseko Y., Kinoshita K., Goshima G., Yanagida M.
    Curr. Biol. 16:1627-1635(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Activity of Cdc2 and its interaction with the cyclin Cdc13 depend on the molecular chaperone Cdc37 in Schizosaccharomyces pombe."
    Turnbull E.L., Martin I.V., Fantes P.A.
    J. Cell Sci. 119:292-302(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDC13 AND CDC37.
  15. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  16. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-15 AND THR-167, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiCDK1_SCHPO
AccessioniPrimary (citable) accession number: P04551
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: February 4, 2015
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.