Reviewed, UniProtKB/Swiss-Prot P04551 (CDC2_SCHPO)

Names and origin

Protein names

Recommended name:
    Cell division control protein 2
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    p34 protein kinase

Gene names

Name:	cdc2
Synonyms:	swo2
ORF Names:	SPBC11B10.09, pi002

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. It is required for entry into S-phase and mitosis. When complexed with cig2, plays a role in G1-S phase transition. When activated and complexed with the cyclin cdc13, it leads to the onset of mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Involved in cell cycle arrest induced by defective RNA splicing. Required for phosphorylation of dis1 to ensure accurate chromosome segregation and for the DNA damage checkpoint. Ref.8 Ref.10 Ref.13 Ref.14
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-167 activates it By similarity.
Subunit structure	Forms a stable but non-covalent complex with a regulatory subunit suc1 and with the cyclin cdc13. Also associates with cdc37 and cig2.
Subcellular location	Cytoplasm Ref.16.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA damage checkpoint Traceable author statement. Source: GeneDB_SPombe DNA replication checkpoint Traceable author statement. Source: GeneDB_SPombe G1/S transition of mitotic cell cycle Ref.9 Traceable author statement. Source: GeneDB_SPombe G2/M transition of mitotic cell cycle Ref.9 Traceable author statement. Source: GeneDB_SPombe cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro traversing start control point of mitotic cell cycle Non-traceable author statement. Source: GeneDB_SPombe
Cellular component	cyclin-dependent protein kinase holoenzyme complex Ref.5 Inferred from physical interaction. Source: GeneDB_SPombe cytosol Ref.16 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Traceable author statement. Source: GeneDB_SPombe protein binding Ref.6 Ref.11 Ref.15 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cdc13	P10815	1	EBI-1187862,EBI-1187843
cdc37	O94740	1	EBI-1187862,EBI-1209056
cig2	P36630	1	EBI-1187862,EBI-1149212
orc2	Q09142	1	EBI-1187862,EBI-2028187
pch1	O74627	1	EBI-1187862,EBI-443575
rum1	P40380	1	EBI-1187862,EBI-1187892

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Cell division control protein 2		PRO_0000085720
Regions
	Domain	4 – 293	290	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	134	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine Ref.5 Ref.12 Ref.17
	Modified residue	167	1	Phosphothreonine Ref.12 Ref.17
Experimental info
	Mutagenesis	15	1	Y → F: Premature entry into mitosis. Ref.7
	Mutagenesis	67	1	C → F in cdc2-4W. Ref.1
	Mutagenesis	67	1	C → Y in cdc2-3W. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P04551-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: D04EF14BA1492875	FASTA	297	34,359
	10 20 30 40 50 60 MENYQKVEKI GEGTYGVVYK ARHKLSGRIV AMKKIRLEDE SEGVPSTAIR EISLLKEVND 70 80 90 100 110 120 ENNRSNCVRL LDILHAESKL YLVFEFLDMD LKKYMDRISE TGATSLDPRL VQKFTYQLVN 130 140 150 160 170 180 GVNFCHSRRI IHRDLKPQNL LIDKEGNLKL ADFGLARSFG VPLRNYTHEI VTLWYRAPEV 190 200 210 220 230 240 LLGSRHYSTG VDIWSVGCIF AEMIRRSPLF PGDSEIDEIF KIFQVLGTPN EEVWPGVTLL 250 260 270 280 290 QDYKSTFPRW KRMDLHKVVP NGEEDAIELL SAMLVYDPAH RISAKRALQQ NYLRDFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases." Hindley J., Phear G.A. Gene 31:129-134(1984) [PubMed: 6526270] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF CYS-67.
[2]	"A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment." Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M. Yeast 16:71-80(2000) [PubMed: 10620777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[4]	"p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase." Brizuela L., Draetta G., Beach D. EMBO J. 6:3507-3515(1987) [PubMed: 3322810] [Abstract] Cited for: ASSOCIATION WITH SUC1.
[5]	"Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis." Gould K.L., Nurse P. Nature 342:39-45(1989) [PubMed: 2682257] [Abstract] Cited for: PHOSPHORYLATION AT TYR-15.
[6]	"Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis." Bueno A., Russell P. Mol. Cell. Biol. 13:2286-2297(1993) [PubMed: 8455610] [Abstract] Cited for: INTERACTION WITH CIG2.
[7]	"Mutational analysis of the fission yeast p34cdc2 protein kinase gene." McNeill S.A., Nurse P. Mol. Gen. Genet. 236:415-426(1993) [PubMed: 8437586] [Abstract] Cited for: MUTAGENESIS OF TYR-15.
[8]	"Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex." Hayles J., Fisher D., Woollard A., Nurse P. Cell 78:813-822(1994) [PubMed: 8087848] [Abstract] Cited for: FUNCTION.
[9]	"A quantitative model for the cdc2 control of S phase and mitosis in fission yeast." Stern B., Nurse P. Trends Genet. 12:345-350(1996) [PubMed: 8855663] [Abstract] Cited for: REVIEW.
[10]	"Cdc2 tyrosine phosphorylation is required for the DNA damage checkpoint in fission yeast." Rhind N., Furnari B., Russell P. Genes Dev. 11:504-511(1997) [PubMed: 9042863] [Abstract] Cited for: FUNCTION.
[11]	"The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases." Yamano H., Kitamura K., Kominami K., Lehmann A., Hunt T., Toda T. Mol. Cell 6:1377-1387(2000) [PubMed: 11163211] [Abstract] Cited for: INTERACTION WITH CIG2.
[12]	"Shotgun identification of protein modifications from protein complexes and lens tissue." MacCoss M.J., McDonald W.H., Saraf A., Sadygov R., Clark J.M., Tasto J.J., Gould K.L., Wolters D., Washburn M., Weiss A., Clark J.I., Yates J.R. III Proc. Natl. Acad. Sci. U.S.A. 99:7900-7905(2002) [PubMed: 12060738] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-15 AND THR-167, MASS SPECTROMETRY.
[13]	"Regulation of Cdc2p and Cdc13p is required for cell cycle arrest induced by defective RNA splicing in fission yeast." Shimada M., Namikawa-Yamada C., Nakanishi M., Murakami H. J. Biol. Chem. 280:32640-32648(2005) [PubMed: 16049013] [Abstract] Cited for: FUNCTION.
[14]	"CDC2 phosphorylation of the fission yeast dis1 ensures accurate chromosome segregation." Aoki K., Nakaseko Y., Kinoshita K., Goshima G., Yanagida M. Curr. Biol. 16:1627-1635(2006) [PubMed: 16920624] [Abstract] Cited for: FUNCTION.
[15]	"Activity of Cdc2 and its interaction with the cyclin Cdc13 depend on the molecular chaperone Cdc37 in Schizosaccharomyces pombe." Turnbull E.L., Martin I.V., Fantes P.A. J. Cell Sci. 119:292-302(2006) [PubMed: 16390871] [Abstract] Cited for: INTERACTION WITH CDC13 AND CDC37.
[16]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[17]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-15 AND THR-167, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12912 Genomic DNA. Translation: AAA35293.1. AB004534 Genomic DNA. Translation: BAA21379.1. CU329671 Genomic DNA. Translation: CAC37513.1.
PIR	TVZP2. A23359.
RefSeq	NP_595629.1.
3D structure databases
SMR	P04551. Positions 1-295.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1076N.
IntAct	P04551. 6 interactions.
STRING	P04551.
Genome annotation databases
GeneID	2539869.
GenomeReviews	Gene locus cdc2 in contig CU329671_GR.
KEGG	spo:SPBC11B10.09.
NMPDR	fig|4896.1.peg.1495.
Organism-specific databases
GeneDB_Spombe	SPBC11B10.09.
Phylogenomic databases
eggNOG	fuNOG04375.
HOGENOM	HBG755340.
OMA	KEVNDEN.
OrthoDB	EOG90CK04.
PhylomeDB	P04551.
Enzyme and pathway databases
BRENDA	2.7.11.22. 653. 2.7.11.23. 653.
Gene expression databases
ArrayExpress	P04551.
GermOnline	SPACTOKYO_453.34. Schizosaccharomyces pombe.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDC2_SCHPO

Accession

Primary (citable) accession number: P04551

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	January 19, 2010
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)