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Protein

Cyclin-dependent kinase 1

Gene

cdc2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required for entry into S-phase and mitosis. When complexed with cig2, plays a role in G1-S phase transition. When activated and complexed with the cyclin cdc13, it leads to the onset of mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Involved in cell cycle arrest induced by defective RNA splicing. Required for phosphorylation of dis1 to ensure accurate chromosome segregation and for the DNA damage checkpoint.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-167 activates it.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei134Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: GO_Central
  • correction of merotelic kinetochore attachment, mitotic Source: PomBase
  • histone phosphorylation Source: GO_Central
  • negative regulation of anaphase-promoting complex-dependent catabolic process Source: PomBase
  • negative regulation of attachment of mitotic spindle microtubules to kinetochore Source: PomBase
  • negative regulation of conjugation with cellular fusion Source: PomBase
  • negative regulation of double-strand break repair via nonhomologous end joining Source: PomBase
  • negative regulation of exit from mitosis Source: PomBase
  • negative regulation of mitotic DNA replication initiation Source: PomBase
  • negative regulation of mitotic spindle elongation Source: PomBase
  • negative regulation of mitotic telomere tethering at nuclear periphery Source: PomBase
  • positive regulation of double-strand break repair via homologous recombination Source: PomBase
  • positive regulation of exit from mitosis Source: PomBase
  • positive regulation of G1/S transition of mitotic cell cycle Source: PomBase
  • positive regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • positive regulation of meiotic cell cycle Source: PomBase
  • positive regulation of mitotic cell cycle DNA replication Source: PomBase
  • positive regulation of mitotic chromosome condensation Source: PomBase
  • positive regulation of mitotic metaphase/anaphase transition Source: PomBase
  • positive regulation of protein import into nucleus Source: PomBase
  • positive regulation of septation initiation signaling Source: PomBase
  • regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • regulation of gene expression Source: GO_Central
  • response to intra-S DNA damage checkpoint signaling Source: PomBase
  • response to mitotic DNA integrity checkpoint signaling Source: PomBase
  • response to mitotic G2 DNA damage checkpoint signaling Source: PomBase
  • traversing start control point of mitotic cell cycle Source: PomBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 5613.
ReactomeiR-SPO-68962. Activation of the pre-replicative complex.
R-SPO-69017. CDK-mediated phosphorylation and removal of Cdc6.
SABIO-RKiP04551.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1
Alternative name(s):
Cell division control protein 2
Cell division protein kinase 1
p34 protein kinase
Gene namesi
Name:cdc2
Synonyms:cdk1, swo2
ORF Names:pi002, SPBC11B10.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC11B10.09.
PomBaseiSPBC11B10.09. cdc2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15Y → F: Premature entry into mitosis. 1 Publication1
Mutagenesisi67C → F in cdc2-4W. 1 Publication1
Mutagenesisi67C → Y in cdc2-3W. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857201 – 297Cyclin-dependent kinase 1Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphothreonineBy similarity1
Modified residuei15Phosphotyrosine2 Publications1
Modified residuei167Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04551.
PRIDEiP04551.

PTM databases

iPTMnetiP04551.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit suc1 and with the cyclin cdc13. Also associates with cdc37 and cig2.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi276415. 98 interactors.
DIPiDIP-1076N.
IntActiP04551. 6 interactors.
MINTiMINT-4686713.
STRINGi4896.SPBC11B10.09.1.

Structurei

3D structure databases

ProteinModelPortaliP04551.
SMRiP04551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST290

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000233024.
InParanoidiP04551.
KOiK04563.
OMAiEMMLVYD.
OrthoDBiEOG092C2FL8.
PhylomeDBiP04551.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P04551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENYQKVEKI GEGTYGVVYK ARHKLSGRIV AMKKIRLEDE SEGVPSTAIR
60 70 80 90 100
EISLLKEVND ENNRSNCVRL LDILHAESKL YLVFEFLDMD LKKYMDRISE
110 120 130 140 150
TGATSLDPRL VQKFTYQLVN GVNFCHSRRI IHRDLKPQNL LIDKEGNLKL
160 170 180 190 200
ADFGLARSFG VPLRNYTHEI VTLWYRAPEV LLGSRHYSTG VDIWSVGCIF
210 220 230 240 250
AEMIRRSPLF PGDSEIDEIF KIFQVLGTPN EEVWPGVTLL QDYKSTFPRW
260 270 280 290
KRMDLHKVVP NGEEDAIELL SAMLVYDPAH RISAKRALQQ NYLRDFH
Length:297
Mass (Da):34,359
Last modified:August 13, 1987 - v1
Checksum:iD04EF14BA1492875
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12912 Genomic DNA. Translation: AAA35293.1.
AB004534 Genomic DNA. Translation: BAA21379.1.
CU329671 Genomic DNA. Translation: CAC37513.1.
PIRiA23359. TVZP2.
RefSeqiNP_595629.1. NM_001021523.2.

Genome annotation databases

EnsemblFungiiSPBC11B10.09.1; SPBC11B10.09.1:pep; SPBC11B10.09.
GeneIDi2539869.
KEGGispo:SPBC11B10.09.

Similar proteinsi

Entry informationi

Entry nameiCDK1_SCHPO
AccessioniPrimary (citable) accession number: P04551
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 25, 2017
This is version 185 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families