Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P04547 (GSTB_BPT4) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA beta-glucosyltransferase

Short name=BGT
Short name=Beta-GT
EC=2.4.1.27
Gene names
Name:bgt
OrganismEnterobacteria phage T4 (Bacteriophage T4) [Reference proteome]
Taxonomic identifier10665 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA. Is involved in a DNA modification process to protect the phage genome against its own nucleases and the host restriction endonuclease system.

Catalytic activity

Transfers a beta-D-glucosyl residue from UDP-glucose to an hydroxymethylcytosine residue in DNA.

Pathway

Genetic information processing; DNA modification.

Subunit structure

Monomer.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA modification

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionDNA beta-glucosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351DNA beta-glucosyltransferase
PRO_0000164942

Experimental info

Sequence conflict85 – 884KFMA → NLWQ in AAA88469. Ref.3

Secondary structure

....................................................................... 351
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04547 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: D1F42B5FE6CB9D61

FASTA35140,666
        10         20         30         40         50         60 
MKIAIINMGN NVINFKTVPS SETIYLFKVI SEMGLNVDII SLKNGVYTKS FDEVDVNDYD 

        70         80         90        100        110        120 
RLIVVNSSIN FFGGKPNLAI LSAQKFMAKY KSKIYYLFTD IRLPFSQSWP NVKNRPWAYL 

       130        140        150        160        170        180 
YTEEELLIKS PIKVISQGIN LDIAKAAHKK VDNVIEFEYF PIEQYKIHMN DFQLSKPTKK 

       190        200        210        220        230        240 
TLDVIYGGSF RSGQRESKMV EFLFDTGLNI EFFGNAREKQ FKNPKYPWTK APVFTGKIPM 

       250        260        270        280        290        300 
NMVSEKNSQA IAALIIGDKN YNDNFITLRV WETMASDAVM LIDEEFDTKH RIINDARFYV 

       310        320        330        340        350 
NNRAELIDRV NELKHSDVLR KEMLSIQHDI LNKTRAKKAE WQDAFKKAID L 

« Hide

References

« Hide 'large scale' references
[1]"T4-induced alpha- and beta-glucosyltransferase: cloning of the genes and a comparison of their products based on sequencing data."
Tomaschewski J., Gram H., Crabb J.W., Rueger W.
Nucleic Acids Res. 13:7551-7568(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Bacteriophage T4 genome."
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.
Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene."
Thylen C.
J. Bacteriol. 170:1994-1998(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-88.
[4]"Nucleotide sequence of the deoxycytidylate hydroxymethylase gene of bacteriophage T4 (g42) and the homology of its gene product with thymidylate synthase of E. coli."
Lamm N., Tomaschewski J., Rueger W.
Nucleic Acids Res. 15:3920-3920(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
[5]"Identification of a family of bacteriophage T4 genes encoding proteins similar to those present in group I introns of fungi and phage."
Sharma M., Ellis R.L., Hinton D.M.
Proc. Natl. Acad. Sci. U.S.A. 89:6658-6662(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 239-351.
[6]"Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose."
Vrelink A., Rueger W., Driessen H.P.C., Freemont P.S.
EMBO J. 13:3413-3422(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[7]"T4 phage beta-glucosyltransferase: substrate binding and proposed catalytic mechanism."
Morera S., Imberty A., Aschke-Sonnenborn U., Ruger W., Freemont P.S.
J. Mol. Biol. 292:717-730(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03139 Genomic DNA. Translation: CAA26908.1.
AF158101 Genomic DNA. Translation: AAD42545.1.
M22767 Genomic DNA. Translation: AAA88469.1.
M69268 Genomic DNA. Translation: AAA32544.1.
Y00148 Genomic DNA. Translation: CAA68343.1.
PIRXUBPB4. A00576.
RefSeqNP_049658.1. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BGTX-ray2.20A1-351[»]
1BGUX-ray2.20A1-351[»]
1C3JX-ray1.88A1-351[»]
1IXYX-ray2.50A/B1-351[»]
1J39X-ray1.87A1-351[»]
1JEJX-ray2.50A1-351[»]
1JG6X-ray1.90A1-351[»]
1JG7X-ray1.65A1-351[»]
1JIUX-ray2.50A1-351[»]
1JIVX-ray2.07A1-351[»]
1JIXX-ray1.65A1-351[»]
1M5RX-ray1.80A/B1-351[»]
1NVKX-ray1.80A1-351[»]
1NZDX-ray2.00A1-351[»]
1NZFX-ray2.10A1-351[»]
1QKJX-ray2.30A1-351[»]
1SXPX-ray2.50A/B1-351[»]
1SXQX-ray1.80A/B1-351[»]
2BGTX-ray2.20A1-351[»]
2BGUX-ray2.20A1-351[»]
ProteinModelPortalP04547.
SMRP04547. Positions 1-351.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT63. Glycosyltransferase Family 63.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1258765.

Enzyme and pathway databases

UniPathwayUPA00198.

Family and domain databases

InterProIPR015281. Phage_T4_Bgt.
[Graphical view]
PfamPF09198. T4-Gluco-transf. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP04547.

Entry information

Entry nameGSTB_BPT4
AccessionPrimary (citable) accession number: P04547
Secondary accession number(s): Q38417
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 16, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways