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Protein

DNA beta-glucosyltransferase

Gene

bgt

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine of T4 DNA to yield glucosyl 5-hydroxymethyl cytosine (glc-HMC) (PubMed:22229759). This DNA process seems to occur immediately after DNA synthesis since the DNA alpha-glucosyltransferase interacts with the clamp protein gp45 (PubMed:22229759). The glc-HMC modification protects the phage genome against its own nucleases and the host restriction endonuclease system (PubMed:22229759). The glc-HMC modification also protects against the host CRISPR-Cas9 defense system (PubMed:26081634).2 Publications

Catalytic activityi

Transfers a beta-D-glucosyl residue from UDP-glucose to a hydroxymethylcytosine residue in DNA.1 Publication

Pathwayi: DNA modification

This protein is involved in the pathway DNA modification, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA modification and in Genetic information processing.

GO - Molecular functioni

  • DNA beta-glucosyltransferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Host-virus interaction, Restriction-modification system evasion by virus

Enzyme and pathway databases

BRENDAi2.4.1.27. 732.
UniPathwayiUPA00198.

Protein family/group databases

CAZyiGT63. Glycosyltransferase Family 63.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA beta-glucosyltransferase (EC:2.4.1.271 Publication)
Short name:
BGT
Short name:
Beta-GT
Gene namesi
Name:bgt
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001649421 – 351DNA beta-glucosyltransferaseAdd BLAST351

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1351
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Beta strandi14 – 17Combined sources4
Helixi18 – 32Combined sources15
Beta strandi37 – 43Combined sources7
Beta strandi46 – 50Combined sources5
Helixi51 – 53Combined sources3
Helixi56 – 58Combined sources3
Beta strandi60 – 65Combined sources6
Beta strandi73 – 75Combined sources3
Helixi78 – 89Combined sources12
Beta strandi94 – 98Combined sources5
Helixi109 – 112Combined sources4
Helixi118 – 120Combined sources3
Helixi123 – 126Combined sources4
Beta strandi132 – 137Combined sources6
Helixi142 – 147Combined sources6
Turni148 – 150Combined sources3
Beta strandi152 – 159Combined sources8
Helixi162 – 164Combined sources3
Helixi165 – 168Combined sources4
Beta strandi169 – 171Combined sources3
Beta strandi182 – 187Combined sources6
Helixi191 – 193Combined sources3
Helixi196 – 203Combined sources8
Beta strandi210 – 215Combined sources6
Helixi218 – 220Combined sources3
Beta strandi233 – 235Combined sources3
Helixi240 – 242Combined sources3
Helixi243 – 247Combined sources5
Beta strandi250 – 255Combined sources6
Helixi259 – 261Combined sources3
Turni262 – 264Combined sources3
Helixi268 – 274Combined sources7
Beta strandi276 – 283Combined sources8
Helixi284 – 286Combined sources3
Helixi296 – 298Combined sources3
Beta strandi299 – 302Combined sources4
Helixi303 – 315Combined sources13
Helixi317 – 335Combined sources19
Helixi338 – 348Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BGTX-ray2.20A1-351[»]
1BGUX-ray2.20A1-351[»]
1C3JX-ray1.88A1-351[»]
1IXYX-ray2.50A/B1-351[»]
1J39X-ray1.87A1-351[»]
1JEJX-ray2.50A1-351[»]
1JG6X-ray1.90A1-351[»]
1JG7X-ray1.65A1-351[»]
1JIUX-ray2.50A1-351[»]
1JIVX-ray2.07A1-351[»]
1JIXX-ray1.65A1-351[»]
1M5RX-ray1.80A/B1-351[»]
1NVKX-ray1.80A1-351[»]
1NZDX-ray2.00A1-351[»]
1NZFX-ray2.10A1-351[»]
1QKJX-ray2.30A1-351[»]
1SXPX-ray2.50A/B1-351[»]
1SXQX-ray1.80A/B1-351[»]
2BGTX-ray2.20A1-351[»]
2BGUX-ray2.20A1-351[»]
ProteinModelPortaliP04547.
SMRiP04547.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04547.

Family & Domainsi

Family and domain databases

InterProiIPR015281. Phage_Bgt.
[Graphical view]
PfamiPF09198. T4-Gluco-transf. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAIINMGN NVINFKTVPS SETIYLFKVI SEMGLNVDII SLKNGVYTKS
60 70 80 90 100
FDEVDVNDYD RLIVVNSSIN FFGGKPNLAI LSAQKFMAKY KSKIYYLFTD
110 120 130 140 150
IRLPFSQSWP NVKNRPWAYL YTEEELLIKS PIKVISQGIN LDIAKAAHKK
160 170 180 190 200
VDNVIEFEYF PIEQYKIHMN DFQLSKPTKK TLDVIYGGSF RSGQRESKMV
210 220 230 240 250
EFLFDTGLNI EFFGNAREKQ FKNPKYPWTK APVFTGKIPM NMVSEKNSQA
260 270 280 290 300
IAALIIGDKN YNDNFITLRV WETMASDAVM LIDEEFDTKH RIINDARFYV
310 320 330 340 350
NNRAELIDRV NELKHSDVLR KEMLSIQHDI LNKTRAKKAE WQDAFKKAID

L
Length:351
Mass (Da):40,666
Last modified:August 13, 1987 - v1
Checksum:iD1F42B5FE6CB9D61
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85 – 88KFMA → NLWQ in AAA88469 (PubMed:2832395).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03139 Genomic DNA. Translation: CAA26908.1.
AF158101 Genomic DNA. Translation: AAD42545.1.
M22767 Genomic DNA. Translation: AAA88469.1.
M69268 Genomic DNA. Translation: AAA32544.1.
Y00148 Genomic DNA. Translation: CAA68343.1.
PIRiA00576. XUBPB4.
RefSeqiNP_049658.1. NC_000866.4.

Genome annotation databases

GeneIDi1258765.
KEGGivg:1258765.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03139 Genomic DNA. Translation: CAA26908.1.
AF158101 Genomic DNA. Translation: AAD42545.1.
M22767 Genomic DNA. Translation: AAA88469.1.
M69268 Genomic DNA. Translation: AAA32544.1.
Y00148 Genomic DNA. Translation: CAA68343.1.
PIRiA00576. XUBPB4.
RefSeqiNP_049658.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BGTX-ray2.20A1-351[»]
1BGUX-ray2.20A1-351[»]
1C3JX-ray1.88A1-351[»]
1IXYX-ray2.50A/B1-351[»]
1J39X-ray1.87A1-351[»]
1JEJX-ray2.50A1-351[»]
1JG6X-ray1.90A1-351[»]
1JG7X-ray1.65A1-351[»]
1JIUX-ray2.50A1-351[»]
1JIVX-ray2.07A1-351[»]
1JIXX-ray1.65A1-351[»]
1M5RX-ray1.80A/B1-351[»]
1NVKX-ray1.80A1-351[»]
1NZDX-ray2.00A1-351[»]
1NZFX-ray2.10A1-351[»]
1QKJX-ray2.30A1-351[»]
1SXPX-ray2.50A/B1-351[»]
1SXQX-ray1.80A/B1-351[»]
2BGTX-ray2.20A1-351[»]
2BGUX-ray2.20A1-351[»]
ProteinModelPortaliP04547.
SMRiP04547.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT63. Glycosyltransferase Family 63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258765.
KEGGivg:1258765.

Enzyme and pathway databases

UniPathwayiUPA00198.
BRENDAi2.4.1.27. 732.

Miscellaneous databases

EvolutionaryTraceiP04547.

Family and domain databases

InterProiIPR015281. Phage_Bgt.
[Graphical view]
PfamiPF09198. T4-Gluco-transf. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTB_BPT4
AccessioniPrimary (citable) accession number: P04547
Secondary accession number(s): Q38417
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.