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Protein

DNA beta-glucosyltransferase

Gene

bgt

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of glucose from uridine diphosphoglucose to 5-hydroxymethyl cytosine of T4 DNA to yield glucosyl 5-hydroxymethyl cytosine (glc-HMC) (PubMed:22229759). This DNA process seems to occur immediately after DNA synthesis since the DNA alpha-glucosyltransferase interacts with the clamp protein gp45 (PubMed:22229759). The glc-HMC modification protects the phage genome against its own nucleases and the host restriction endonuclease system (PubMed:22229759). The glc-HMC modification also protects against the host CRISPR-Cas9 defense system (PubMed:26081634).2 Publications

Catalytic activityi

Transfers a beta-D-glucosyl residue from UDP-glucose to a hydroxymethylcytosine residue in DNA.1 Publication

Pathwayi: DNA modification

This protein is involved in the pathway DNA modification, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA modification and in Genetic information processing.

GO - Molecular functioni

  • DNA beta-glucosyltransferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Host-virus interaction, Restriction-modification system evasion by virus

Enzyme and pathway databases

BRENDAi2.4.1.27. 732.
UniPathwayiUPA00198.

Protein family/group databases

CAZyiGT63. Glycosyltransferase Family 63.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA beta-glucosyltransferase (EC:2.4.1.271 Publication)
Short name:
BGT
Short name:
Beta-GT
Gene namesi
Name:bgt
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351DNA beta-glucosyltransferasePRO_0000164942Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
351
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 108Combined sources
Beta strandi14 – 174Combined sources
Helixi18 – 3215Combined sources
Beta strandi37 – 437Combined sources
Beta strandi46 – 505Combined sources
Helixi51 – 533Combined sources
Helixi56 – 583Combined sources
Beta strandi60 – 656Combined sources
Beta strandi73 – 753Combined sources
Helixi78 – 8912Combined sources
Beta strandi94 – 985Combined sources
Helixi109 – 1124Combined sources
Helixi118 – 1203Combined sources
Helixi123 – 1264Combined sources
Beta strandi132 – 1376Combined sources
Helixi142 – 1476Combined sources
Turni148 – 1503Combined sources
Beta strandi152 – 1598Combined sources
Helixi162 – 1643Combined sources
Helixi165 – 1684Combined sources
Beta strandi169 – 1713Combined sources
Beta strandi182 – 1876Combined sources
Helixi191 – 1933Combined sources
Helixi196 – 2038Combined sources
Beta strandi210 – 2156Combined sources
Helixi218 – 2203Combined sources
Beta strandi233 – 2353Combined sources
Helixi240 – 2423Combined sources
Helixi243 – 2475Combined sources
Beta strandi250 – 2556Combined sources
Helixi259 – 2613Combined sources
Turni262 – 2643Combined sources
Helixi268 – 2747Combined sources
Beta strandi276 – 2838Combined sources
Helixi284 – 2863Combined sources
Helixi296 – 2983Combined sources
Beta strandi299 – 3024Combined sources
Helixi303 – 31513Combined sources
Helixi317 – 33519Combined sources
Helixi338 – 34811Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BGTX-ray2.20A1-351[»]
1BGUX-ray2.20A1-351[»]
1C3JX-ray1.88A1-351[»]
1IXYX-ray2.50A/B1-351[»]
1J39X-ray1.87A1-351[»]
1JEJX-ray2.50A1-351[»]
1JG6X-ray1.90A1-351[»]
1JG7X-ray1.65A1-351[»]
1JIUX-ray2.50A1-351[»]
1JIVX-ray2.07A1-351[»]
1JIXX-ray1.65A1-351[»]
1M5RX-ray1.80A/B1-351[»]
1NVKX-ray1.80A1-351[»]
1NZDX-ray2.00A1-351[»]
1NZFX-ray2.10A1-351[»]
1QKJX-ray2.30A1-351[»]
1SXPX-ray2.50A/B1-351[»]
1SXQX-ray1.80A/B1-351[»]
2BGTX-ray2.20A1-351[»]
2BGUX-ray2.20A1-351[»]
ProteinModelPortaliP04547.
SMRiP04547. Positions 1-351.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04547.

Family & Domainsi

Family and domain databases

InterProiIPR015281. Phage_Bgt.
[Graphical view]
PfamiPF09198. T4-Gluco-transf. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAIINMGN NVINFKTVPS SETIYLFKVI SEMGLNVDII SLKNGVYTKS
60 70 80 90 100
FDEVDVNDYD RLIVVNSSIN FFGGKPNLAI LSAQKFMAKY KSKIYYLFTD
110 120 130 140 150
IRLPFSQSWP NVKNRPWAYL YTEEELLIKS PIKVISQGIN LDIAKAAHKK
160 170 180 190 200
VDNVIEFEYF PIEQYKIHMN DFQLSKPTKK TLDVIYGGSF RSGQRESKMV
210 220 230 240 250
EFLFDTGLNI EFFGNAREKQ FKNPKYPWTK APVFTGKIPM NMVSEKNSQA
260 270 280 290 300
IAALIIGDKN YNDNFITLRV WETMASDAVM LIDEEFDTKH RIINDARFYV
310 320 330 340 350
NNRAELIDRV NELKHSDVLR KEMLSIQHDI LNKTRAKKAE WQDAFKKAID

L
Length:351
Mass (Da):40,666
Last modified:August 13, 1987 - v1
Checksum:iD1F42B5FE6CB9D61
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 884KFMA → NLWQ in AAA88469 (PubMed:2832395).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03139 Genomic DNA. Translation: CAA26908.1.
AF158101 Genomic DNA. Translation: AAD42545.1.
M22767 Genomic DNA. Translation: AAA88469.1.
M69268 Genomic DNA. Translation: AAA32544.1.
Y00148 Genomic DNA. Translation: CAA68343.1.
PIRiA00576. XUBPB4.
RefSeqiNP_049658.1. NC_000866.4.

Genome annotation databases

GeneIDi1258765.
KEGGivg:1258765.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03139 Genomic DNA. Translation: CAA26908.1.
AF158101 Genomic DNA. Translation: AAD42545.1.
M22767 Genomic DNA. Translation: AAA88469.1.
M69268 Genomic DNA. Translation: AAA32544.1.
Y00148 Genomic DNA. Translation: CAA68343.1.
PIRiA00576. XUBPB4.
RefSeqiNP_049658.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BGTX-ray2.20A1-351[»]
1BGUX-ray2.20A1-351[»]
1C3JX-ray1.88A1-351[»]
1IXYX-ray2.50A/B1-351[»]
1J39X-ray1.87A1-351[»]
1JEJX-ray2.50A1-351[»]
1JG6X-ray1.90A1-351[»]
1JG7X-ray1.65A1-351[»]
1JIUX-ray2.50A1-351[»]
1JIVX-ray2.07A1-351[»]
1JIXX-ray1.65A1-351[»]
1M5RX-ray1.80A/B1-351[»]
1NVKX-ray1.80A1-351[»]
1NZDX-ray2.00A1-351[»]
1NZFX-ray2.10A1-351[»]
1QKJX-ray2.30A1-351[»]
1SXPX-ray2.50A/B1-351[»]
1SXQX-ray1.80A/B1-351[»]
2BGTX-ray2.20A1-351[»]
2BGUX-ray2.20A1-351[»]
ProteinModelPortaliP04547.
SMRiP04547. Positions 1-351.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT63. Glycosyltransferase Family 63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258765.
KEGGivg:1258765.

Enzyme and pathway databases

UniPathwayiUPA00198.
BRENDAi2.4.1.27. 732.

Miscellaneous databases

EvolutionaryTraceiP04547.

Family and domain databases

InterProiIPR015281. Phage_Bgt.
[Graphical view]
PfamiPF09198. T4-Gluco-transf. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTB_BPT4
AccessioniPrimary (citable) accession number: P04547
Secondary accession number(s): Q38417
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: June 8, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.