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Reviewed, UniProtKB/Swiss-Prot P04547 (GSTB_BPT4)

Last modified June 16, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    DNA beta-glucosyltransferase
      Short name=Beta-GT
      Short name=BGT
    EC=2.4.1.27
Gene names
Name: bgt
OrganismEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifier10665 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeT4-like viruses
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA. Is involved in a DNA modification process to protect the phage genome against its own nucleases and the host restriction endonuclease system.

Catalytic activity

Transfers a beta-D-glucosyl residue from UDP-glucose to an hydroxymethylcytosine residue in DNA.

Pathway

Genetic information processing; DNA modification.

Subunit structure

Monomer.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   Technical term3D-structure
Gene Ontology (GO)
   Molecular functionDNA beta-glucosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351DNA beta-glucosyltransferase
PRO_0000164942

Experimental info

Sequence conflict85 – 884KFMA → NLWQ in AAA88469. Ref.3

Secondary structure

................................................................. 351
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04547-1 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: D1F42B5FE6CB9D61

FASTA35140,666
        10         20         30         40         50         60 
MKIAIINMGN NVINFKTVPS SETIYLFKVI SEMGLNVDII SLKNGVYTKS FDEVDVNDYD 

        70         80         90        100        110        120 
RLIVVNSSIN FFGGKPNLAI LSAQKFMAKY KSKIYYLFTD IRLPFSQSWP NVKNRPWAYL 

       130        140        150        160        170        180 
YTEEELLIKS PIKVISQGIN LDIAKAAHKK VDNVIEFEYF PIEQYKIHMN DFQLSKPTKK 

       190        200        210        220        230        240 
TLDVIYGGSF RSGQRESKMV EFLFDTGLNI EFFGNAREKQ FKNPKYPWTK APVFTGKIPM 

       250        260        270        280        290        300 
NMVSEKNSQA IAALIIGDKN YNDNFITLRV WETMASDAVM LIDEEFDTKH RIINDARFYV 

       310        320        330        340        350 
NNRAELIDRV NELKHSDVLR KEMLSIQHDI LNKTRAKKAE WQDAFKKAID L 

« Hide

References

« Hide 'large scale' references
[1]"T4-induced alpha- and beta-glucosyltransferase: cloning of the genes and a comparison of their products based on sequencing data."
Tomaschewski J., Gram H., Crabb J.W., Rueger W.
Nucleic Acids Res. 13:7551-7568(1985) [PubMed: 2999696] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Bacteriophage T4 genome."
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.
Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed: 12626685] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Expression and DNA sequence of the cloned bacteriophage T4 dCMP hydroxymethylase gene."
Thylen C.
J. Bacteriol. 170:1994-1998(1988) [PubMed: 2832395] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-88.
[4]"Nucleotide sequence of the deoxycytidylate hydroxymethylase gene of bacteriophage T4 (g42) and the homology of its gene product with thymidylate synthase of E. coli."
Lamm N., Tomaschewski J., Rueger W.
Nucleic Acids Res. 15:3920-3920(1987) [PubMed: 3295783] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
[5]"Identification of a family of bacteriophage T4 genes encoding proteins similar to those present in group I introns of fungi and phage."
Sharma M., Ellis R.L., Hinton D.M.
Proc. Natl. Acad. Sci. U.S.A. 89:6658-6662(1992) [PubMed: 1631169] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 239-351.
[6]"Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose."
Vrelink A., Rueger W., Driessen H.P.C., Freemont P.S.
EMBO J. 13:3413-3422(1994) [PubMed: 8062817] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[7]"T4 phage beta-glucosyltransferase: substrate binding and proposed catalytic mechanism."
Morera S., Imberty A., Aschke-Sonnenborn U., Ruger W., Freemont P.S.
J. Mol. Biol. 292:717-730(1999) [PubMed: 10497034] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

X03139 Genomic DNA. Translation: CAA26908.1.
AF158101 Genomic DNA. Translation: AAD42545.1.
M22767 Genomic DNA. Translation: AAA88469.1.
M69268 Genomic DNA. Translation: AAA32544.1.
Y00148 Genomic DNA. Translation: CAA68343.1.
PIRXUBPB4. A00576.
RefSeqNP_049658.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1BGTX-ray2.20A1-351[»]
1BGUX-ray2.20A1-351[»]
1C3JX-ray1.88A1-351[»]
1IXYX-ray2.50A/B1-351[»]
1J39X-ray1.87A1-351[»]
1JEJX-ray2.50A1-351[»]
1JG6X-ray1.90A1-351[»]
1JG7X-ray1.65A1-351[»]
1JIUX-ray2.50A1-351[»]
1JIVX-ray2.07A1-351[»]
1JIXX-ray1.65A1-351[»]
1M5RX-ray1.80A/B1-351[»]
1NVKX-ray1.80A1-351[»]
1NZDX-ray2.00A1-351[»]
1NZFX-ray2.10A1-351[»]
1QKJX-ray2.30A1-351[»]
1SXPX-ray2.50A/B1-351[»]
1SXQX-ray1.80A/B1-351[»]
2BGTX-ray2.20A1-351[»]
2BGUX-ray2.20A1-351[»]
ModBaseSearch...

Protein family/group databases

CAZyGT63. Glycosyltransferase Family 63.

Genome annotation databases

GeneID1258765.
GenomeReviewsGene locus T4p044 in contig AF158101_GR.

Enzyme and pathway databases

BRENDA2.4.1.27. 1108.

Family and domain databases

InterProIPR015281. Phage_T4_glucotransf.
[Graphical view]
PfamPF09198. T4-Gluco-transf. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTB_BPT4
AccessionPrimary (citable) accession number: P04547
Secondary accession number(s): Q38417
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents