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Protein

Non-structural protein 2

Gene

1B

Organism
Human respiratory syncytial virus A (strain A2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in antagonizing the type I IFN-mediated antiviral response. Acts cooperatively with NS1 to repress activation and nuclear translocation of host IFN-regulatory factor IRF-3. Interacts with the host cytoplasmic sensor of viral nucleic acids DDX58/RIG-I and prevents the interaction with its downstream partner MAVS. Mediates the proteasomal degradation of host STAT2 with Elongin-Cullin E3 ligase. Induces activation of NF-kappa-B. Suppresses premature apoptosis by an NF-kappa-B-dependent, interferon-independent mechanism and thus facilitates virus growth. May also inhibit viral transcription and RNA replication.5 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host RIG-I by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2
Short name:
NS2
Alternative name(s):
Non-structural protein 1B
Gene namesi
Name:1B
Synonyms:NS2
OrganismiHuman respiratory syncytial virus A (strain A2)
Taxonomic identifieri11259 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesParamyxoviridaePneumovirinaePneumovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007678 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 124124Non-structural protein 2PRO_0000142789Add
BLAST

Interactioni

Subunit structurei

Homomultimerizes. Interacts with host DDX58; this interaction prevents host signaling pathway involved in interferon production.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DDX58O957862EBI-3648048,EBI-995350From a different organism.

Protein-protein interaction databases

IntActiP04543. 1 interaction.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR004336. RSV_NS2.
[Graphical view]
PfamiPF03113. RSV_NS2. 1 hit.
[Graphical view]
ProDomiPD009327. RSV_NS2. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P04543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTTHNDNTP QRLMITDMRP LSLETIITSL TRDIITHKFI YLINHECIVR
60 70 80 90 100
KLDERQATFT FLVNYEMKLL HKVGSTKYKK YTEYNTKYGT FPMPIFINHD
110 120
GFLECIGIKP TKHTPIIYKY DLNP
Length:124
Mass (Da):14,702
Last modified:July 15, 1998 - v2
Checksum:iC14DF0FE9C9E5512
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551R → K in AAB59851 (PubMed:2998021).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11486 Genomic RNA. Translation: AAB59851.1.
U50362 Genomic RNA. Translation: AAB86657.1.
U50363 Genomic RNA. Translation: AAB86669.1.
U63644 Genomic RNA. Translation: AAC55963.1.
AF035006 Genomic RNA. Translation: AAC14895.1.
PIRiB94336. MNNZ1B.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11486 Genomic RNA. Translation: AAB59851.1.
U50362 Genomic RNA. Translation: AAB86657.1.
U50363 Genomic RNA. Translation: AAB86669.1.
U63644 Genomic RNA. Translation: AAC55963.1.
AF035006 Genomic RNA. Translation: AAC14895.1.
PIRiB94336. MNNZ1B.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP04543. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR004336. RSV_NS2.
[Graphical view]
PfamiPF03113. RSV_NS2. 1 hit.
[Graphical view]
ProDomiPD009327. RSV_NS2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequences of the 1B and 1C nonstructural protein mRNAs of human respiratory syncytial virus."
    Collins P.L., Wertz G.W.
    Virology 143:442-451(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "mRNA sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22K structural protein."
    Elango N., Satake M., Venkatesan S.
    J. Virol. 55:101-110(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "A cold-passaged, attenuated strain of human respiratory syncytial virus contains mutations in the F and L genes."
    Connors M., Crowe J.E. Jr., Firestone C.Y., Murphy B.R., Collins P.L.
    Virology 208:478-484(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. "Acquisition of the ts phenotype by a chemically mutagenized cold-passaged human respiratory syncytial virus vaccine candidate results from the acquisition of a single mutation in the polymerase (L) gene."
    Crowe J.E. Jr., Firestone C.Y., Whitehead S.S., Collins P.L., Murphy B.R.
    Virus Genes 13:269-273(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  5. "Recombinant respiratory syncytial virus (RSV) bearing a set of mutations from cold-passaged RSV is attenuated in chimpanzees."
    Whitehead S.S., Juhasz K., Firestone C.Y., Collins P.L., Murphy B.R.
    J. Virol. 72:4467-4471(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Cold-passage attenuated.
  6. "Expression and characterisation of the NS1 and NS2 proteins of respiratory syncytial virus."
    Evans J.E., Cane P.A., Pringle C.R.
    Virus Res. 43:155-161(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: HOMOMULTIMERIZATION.
  7. "Suppression of the induction of alpha, beta, and lambda interferons by the NS1 and NS2 proteins of human respiratory syncytial virus in human epithelial cells and macrophages."
    Spann K.M., Tran K.C., Chi B., Rabin R.L., Collins P.L.
    J. Virol. 78:4363-4369(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Effects of nonstructural proteins NS1 and NS2 of human respiratory syncytial virus on interferon regulatory factor 3, NF-kappaB, and proinflammatory cytokines."
    Spann K.M., Tran K.C., Collins P.L.
    J. Virol. 79:5353-5362(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Nonstructural proteins of respiratory syncytial virus suppress premature apoptosis by an NF-kappaB-dependent, interferon-independent mechanism and facilitate virus growth."
    Bitko V., Shulyayeva O., Mazumder B., Musiyenko A., Ramaswamy M., Look D.C., Barik S.
    J. Virol. 81:1786-1795(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN APOPTOSIS SUPPRESSION.
  10. "Respiratory syncytial virus NS1 protein degrades STAT2 by using the Elongin-Cullin E3 ligase."
    Elliott J., Lynch O.T., Suessmuth Y., Qian P., Boyd C.R., Burrows J.F., Buick R., Stevenson N.J., Touzelet O., Gadina M., Power U.F., Johnston J.A.
    J. Virol. 81:3428-3436(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Human respiratory syncytial virus nonstructural protein NS2 antagonizes the activation of beta interferon transcription by interacting with RIG-I."
    Ling Z., Tran K.C., Teng M.N.
    J. Virol. 83:3734-3742(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HOST DDX58.

Entry informationi

Entry nameiNS2_HRSVA
AccessioniPrimary (citable) accession number: P04543
Secondary accession number(s): Q77YB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 15, 1998
Last modified: June 8, 2016
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.