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P04531

- DNMK_BPT4

UniProt

P04531 - DNMK_BPT4

Protein

Deoxynucleotide monophosphate kinase

Gene

1

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Phosphorylates dGMP, dTMP and 5-hydroxymethyl-dCMP while excluding dCMP and dAMP. The phosphorylation of 5-hydroxymethyl-dCMP represents the first step in the replacement of cytosine by hydroxymethylcytosine in new viral DNA genomes.1 Publication

    Catalytic activityi

    ATP + deoxynucleoside phosphate = ADP + deoxynucleoside diphosphate.

    GO - Molecular functioni

    1. (deoxy)nucleoside-phosphate kinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxynucleotide monophosphate kinase (EC:2.7.4.13)
    Short name:
    DNK
    Short name:
    dNMP kinase
    Alternative name(s):
    Gp1
    Gene namesi
    Name:1
    OrganismiEnterobacteria phage T4 (Bacteriophage T4)
    Taxonomic identifieri10665 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000009087: Genome

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 241241Deoxynucleotide monophosphate kinasePRO_0000164948Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    Secondary structure

    1
    241
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 76
    Helixi14 – 2411
    Beta strandi27 – 293
    Helixi34 – 4714
    Turni48 – 503
    Beta strandi51 – 533
    Helixi58 – 614
    Turni62 – 654
    Helixi76 – 9116
    Beta strandi101 – 1066
    Beta strandi108 – 1103
    Helixi112 – 12312
    Helixi131 – 1399
    Turni140 – 1423
    Helixi143 – 1464
    Helixi151 – 16313
    Beta strandi169 – 1735
    Helixi179 – 1879
    Beta strandi191 – 1966
    Helixi207 – 2093
    Beta strandi219 – 2224
    Helixi227 – 23913

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DEKX-ray2.00A/B1-241[»]
    1DELX-ray2.20A/B1-241[»]
    ProteinModelPortaliP04531.
    SMRiP04531. Positions 1-241.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04531.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the dNMP kinase family.Curated

    Family and domain databases

    Gene3Di1.10.238.70. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR023191. DNMP_kinase_N_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P04531-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLIFLSGVK RSGKDTTADF IMSNYSAVKY QLAGPIKDAL AYAWGVFAAN    50
    TDYPCLTRKE FEGIDYDRET NLNLTKLEVI TIMEQAFCYL NGKSPIKGVF 100
    VFDDEGKESV NFVAFNKITD VINNIEDQWS VRRLMQALGT DLIVNNFDRM 150
    YWVKLFALDY LDKFNSGYDY YIVPDTRQDH EMDAARAMGA TVIHVVRPGQ 200
    KSNDTHITEA GLPIRDGDLV ITNDGSLEEL FSKIKNTLKV L 241
    Length:241
    Mass (Da):27,329
    Last modified:August 13, 1987 - v1
    Checksum:i61AD2375CC94BDE8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03016 Genomic DNA. Translation: CAA26800.1.
    AF158101 Genomic DNA. Translation: AAD42414.1.
    X14845 Genomic DNA. Translation: CAA32953.1.
    PIRiC92919. KIBPD4.
    RefSeqiNP_049752.1. NC_000866.4.

    Genome annotation databases

    GeneIDi1258557.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03016 Genomic DNA. Translation: CAA26800.1 .
    AF158101 Genomic DNA. Translation: AAD42414.1 .
    X14845 Genomic DNA. Translation: CAA32953.1 .
    PIRi C92919. KIBPD4.
    RefSeqi NP_049752.1. NC_000866.4.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DEK X-ray 2.00 A/B 1-241 [» ]
    1DEL X-ray 2.20 A/B 1-241 [» ]
    ProteinModelPortali P04531.
    SMRi P04531. Positions 1-241.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    DrugBanki DB00131. Adenosine monophosphate.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1258557.

    Miscellaneous databases

    EvolutionaryTracei P04531.

    Family and domain databases

    Gene3Di 1.10.238.70. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR023191. DNMP_kinase_N_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence organization and control of transcription in the bacteriophage T4 tRNA region."
      Broida J., Abelson J.
      J. Mol. Biol. 185:545-563(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Nucleotide sequence of the bacteriophage T4 gene 57 and a deduced amino acid sequence."
      Herrmann R.
      Nucleic Acids Res. 10:1105-1112(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 148-241.
    4. "Sequencing, cloning and overexpression of genes of bacteriophage T4 between map positions 74.325 and 77.184."
      Koch T., Lamm N., Rueger W.
      Nucleic Acids Res. 17:4392-4392(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
    5. "The enzymology of virus-infected bacteria. X. A biochemical-genetic study of the deoxynucleotide kinase induced by wild type and amber mutants of phage T4."
      Duckworth D.H., Bessman M.J.
      J. Biol. Chem. 242:2877-2885(1967) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Crystal structure of bacteriophage T4 deoxynucleotide kinase with its substrates dGMP and ATP."
      Teplyakov A., Sebastiao P., Obmolova G., Perrakis A., Brush G.S., Bessman M.J., Wilson K.S.
      EMBO J. 15:3487-3497(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

    Entry informationi

    Entry nameiDNMK_BPT4
    AccessioniPrimary (citable) accession number: P04531
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3