P04531 (DNMK_BPT4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxynucleotide monophosphate kinase
Short name=DNK
Short name=dNMP kinase
EC=2.7.4.13

Alternative name(s):
Gp1

Gene names

Name:

1

Organism

Enterobacteria phage T4 (Bacteriophage T4) [Reference proteome]

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Tevenvirinae › T4likevirus › Enterobacteria phage T4 sensu lato

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts on dGMP, dTMP and 5-hydroxymethyl-dCMP while excluding dCMP and dAMP.
Catalytic activity	ATP + deoxynucleoside phosphate = ADP + deoxynucleoside diphosphate.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the dNMP kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	(deoxy)nucleoside-phosphate kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 241

241

Deoxynucleotide monophosphate kinase

PRO_0000164948

Secondary structure

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241

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Helix

14 – 24

11

Beta strand

27 – 29

3

Helix

34 – 47

14

Turn

48 – 50

3

Beta strand

51 – 53

3

Helix

58 – 61

4

Turn

62 – 65

4

Helix

76 – 91

16

Beta strand

101 – 106

6

Beta strand

108 – 110

3

Helix

112 – 123

12

Helix

131 – 139

9

Turn

140 – 142

3

Helix

143 – 146

4

Helix

151 – 163

13

Beta strand

169 – 173

5

Helix

179 – 187

9

Beta strand

191 – 196

6

Helix

207 – 209

3

Beta strand

219 – 222

4

Helix

227 – 239

13

Sequences

	Sequence		Length	Mass (Da)
	P04531 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 61AD2375CC94BDE8	FASTA	241	27,329
	10 20 30 40 50 60 MKLIFLSGVK RSGKDTTADF IMSNYSAVKY QLAGPIKDAL AYAWGVFAAN TDYPCLTRKE 70 80 90 100 110 120 FEGIDYDRET NLNLTKLEVI TIMEQAFCYL NGKSPIKGVF VFDDEGKESV NFVAFNKITD 130 140 150 160 170 180 VINNIEDQWS VRRLMQALGT DLIVNNFDRM YWVKLFALDY LDKFNSGYDY YIVPDTRQDH 190 200 210 220 230 240 EMDAARAMGA TVIHVVRPGQ KSNDTHITEA GLPIRDGDLV ITNDGSLEEL FSKIKNTLKV L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence organization and control of transcription in the bacteriophage T4 tRNA region." Broida J., Abelson J. J. Mol. Biol. 185:545-563(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Nucleotide sequence of the bacteriophage T4 gene 57 and a deduced amino acid sequence." Herrmann R. Nucleic Acids Res. 10:1105-1112(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 148-241.
[4]	"Sequencing, cloning and overexpression of genes of bacteriophage T4 between map positions 74.325 and 77.184." Koch T., Lamm N., Rueger W. Nucleic Acids Res. 17:4392-4392(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
[5]	"Crystal structure of bacteriophage T4 deoxynucleotide kinase with its substrates dGMP and ATP." Teplyakov A., Sebastiao P., Obmolova G., Perrakis A., Brush G.S., Bessman M.J., Wilson K.S. EMBO J. 15:3487-3497(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03016 Genomic DNA. Translation: CAA26800.1.
AF158101 Genomic DNA. Translation: AAD42414.1.
X14845 Genomic DNA. Translation: CAA32953.1.

PIR

KIBPD4. C92919.

RefSeq

NP_049752.1. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DEK	X-ray	2.00	A/B	1-241	[»]
1DEL	X-ray	2.20	A/B	1-241	[»]

ProteinModelPortal

P04531.

SMR

P04531. Positions 1-241.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1258557.

Phylogenomic databases

ProtClustDB

PHA2575.

Family and domain databases

Gene3D

1.10.238.70. 1 hit.

InterPro

IPR023191. DNMP_kinase_N_dom.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00131. Adenosine monophosphate.

EvolutionaryTrace

P04531.

Entry information

Entry name

DNMK_BPT4

Accession

Primary (citable) accession number: P04531

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program