P04527 (DPA62_BPT4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase accessory protein 62

Alternative name(s):
Clamp loader small subunit
Protein Gp62

Gene names

Name:

62

Organism

Enterobacteria phage T4 (Bacteriophage T4) [Reference proteome]

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Tevenvirinae › T4likevirus › Enterobacteria phage T4 sensu lato

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sliding clamp (Gp45) loader. Required for elongation of primed templates by DNA polymerase.
Subunit structure	Heterodimer of Gp44 and Gp62 that forms a complex with Gp45 in the presence of ATP.

Ontologies

Keywords
Biological process	DNA replication
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 187

187

DNA polymerase accessory protein 62

PRO_0000164929

Experimental info

Sequence conflict

71

1

Q → E in CAA25342. Ref.2

Secondary structure

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187

Helix Strand Turn

Details...

Helix

12 – 19

8

Helix

22 – 32

11

Helix

36 – 49

14

Beta strand

55 – 58

4

Helix

62 – 70

9

Turn

73 – 75

3

Helix

76 – 84

9

Turn

85 – 88

4

Helix

93 – 101

9

Helix

119 – 131

13

Helix

137 – 147

11

Turn

148 – 151

4

Helix

152 – 157

6

Helix

164 – 170

7

Helix

175 – 182

8

Turn

183 – 185

3

Sequences

	Sequence		Length	Mass (Da)
	P04527 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 686C9E3D28B42E07	FASTA	187	21,363
	10 20 30 40 50 60 MSLFKDDIQL NEHQVAWYSK DWTAVQSAAD SFKEKAENEF FEIIGAINNK TKCSIAQKDY 70 80 90 100 110 120 SKFMVENALS QFPECMPAVY AMNLIGSGLS DEAHFNYLMA AVPRGKRYGK WAKLVEDSTE 130 140 150 160 170 180 VLIIKLLAKR YQVNTNDAIN YKSILTKNGK LPLVLKELKG LVTDDFLKEV TKNVKEQKQL KKLALEW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization and structure of four T4 genes coding for DNA replication proteins." Spicer E.K., Konigsberg W.H. (In) Mathews C.K., Kutter E.M., Mosig G., Berget P.B. (eds.); Bacteriophage T4, pp.291-301, American Society for Microbiology, Washington D.C. (1983) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The 44P subunit of the T4 DNA polymerase accessory protein complex catalyzes ATP hydrolysis." Rush J., Lin T.C., Quinones M., Spicer E.K., Douglas I., Williams K.R., Konigsberg W.H. J. Biol. Chem. 264:10943-10953(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M10160 Genomic DNA. Translation: AAC05395.1.
X00769 Genomic DNA. Translation: CAA25342.1.
AF158101 Genomic DNA. Translation: AAD42513.1.

PIR

IDBPT4. A04303.

RefSeq

NP_049664.1. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3U5Z	X-ray	3.50	A/K	2-187	[»]
3U60	X-ray	3.34	A	2-187	[»]
3U61	X-ray	3.20	A	2-187	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1258559.

Phylogenomic databases

ProtClustDB

PHA2593.

Family and domain databases

ProtoNet

Search...

Other

BindingDB

P04527.

Entry information

Entry name

DPA62_BPT4

Accession

Primary (citable) accession number: P04527

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program