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Protein

Sliding-clamp-loader gp44 subunit

Gene

44

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Function as a sliding-clamp-loading ATPase enzyme during DNA replication. Required for elongation of primed templates by controlling the polymerase processivity. Possesses DNA-dependent ATPase activity on its own and within the heterodimer gp44/gp62. Progressive binding of ATPs triggers a conformational change in the complex that inhibits ATPase activity.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi50 – 578ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • bidirectional double-stranded viral DNA replication Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sliding-clamp-loader gp44 subunit
Alternative name(s):
Clamp loader large subunit
Gene product 44
Short name:
gp44
Gene namesi
Name:44
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Sliding-clamp-loader gp44 subunitPRO_0000164927Add
BLAST

Interactioni

Subunit structurei

Heterodimer of gp44 and gp62 that forms a complex with gp45 in the presence of ATP.

Structurei

Secondary structure

1
319
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 144Combined sources
Turni20 – 223Combined sources
Helixi27 – 3812Combined sources
Beta strandi44 – 485Combined sources
Beta strandi50 – 523Combined sources
Helixi56 – 6611Combined sources
Beta strandi69 – 757Combined sources
Turni76 – 783Combined sources
Helixi81 – 855Combined sources
Helixi87 – 937Combined sources
Beta strandi101 – 1088Combined sources
Helixi112 – 1143Combined sources
Helixi115 – 12814Combined sources
Helixi129 – 1313Combined sources
Beta strandi133 – 1408Combined sources
Helixi141 – 1433Combined sources
Helixi148 – 1514Combined sources
Beta strandi152 – 1565Combined sources
Helixi162 – 18322Combined sources
Beta strandi186 – 1883Combined sources
Helixi190 – 19910Combined sources
Helixi206 – 2149Combined sources
Helixi215 – 2173Combined sources
Beta strandi218 – 2203Combined sources
Helixi222 – 23110Combined sources
Helixi236 – 2438Combined sources
Helixi247 – 25711Combined sources
Beta strandi258 – 2603Combined sources
Helixi261 – 27515Combined sources
Helixi278 – 29417Combined sources
Turni295 – 2973Combined sources
Helixi301 – 31515Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3U5ZX-ray3.50B/C/D/E/L/M/N/O1-319[»]
3U60X-ray3.34B/C/D/E1-319[»]
3U61X-ray3.20B/C/D/E1-319[»]
ProteinModelPortaliP04526.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

To E.coli DNA polymerase III subunits gamma/tau and to human activator 1, 37 and 40 kDa subunits.Curated

Phylogenomic databases

KOiK18944.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P04526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITVNEKEHI LEQKYRPSTI DECILPAFDK ETFKSITSKG KIPHIILHSP
60 70 80 90 100
SPGTGKTTVA KALCHDVNAD MMFVNGSDCK IDFVRGPLTN FASAASFDGR
110 120 130 140 150
QKVIVIDEFD RSGLAESQRH LRSFMEAYSS NCSIIITANN IDGIIKPLQS
160 170 180 190 200
RCRVITFGQP TDEDKIEMMK QMIRRLTEIC KHEGIAIADM KVVAALVKKN
210 220 230 240 250
FPDFRKTIGE LDSYSSKGVL DAGILSLVTN DRGAIDDVLE SLKNKDVKQL
260 270 280 290 300
RALAPKYAAD YSWFVGKLAE EIYSRVTPQS IIRMYEIVGE NNQYHGIAAN
310
TELHLAYLFI QLACEMQWK
Length:319
Mass (Da):35,786
Last modified:August 13, 1987 - v1
Checksum:iB912551C4FF49578
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321C → S in CAA25341 (PubMed:6473098).Curated
Sequence conflicti175 – 1751R → G in CAA25341 (PubMed:6473098).Curated
Sequence conflicti213 – 2131S → T in CAA25341 (PubMed:6473098).Curated
Sequence conflicti275 – 2751R → A in CAA25341 (PubMed:6473098).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00769 Genomic DNA. Translation: CAA25341.1.
M10160 Genomic DNA. Translation: AAC05394.1.
AF158101 Genomic DNA. Translation: AAD42469.1.
PIRiA04302. IDBPA4.
RefSeqiNP_049665.1. NC_000866.4.

Genome annotation databases

GeneIDi1258787.
KEGGivg:1258787.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00769 Genomic DNA. Translation: CAA25341.1.
M10160 Genomic DNA. Translation: AAC05394.1.
AF158101 Genomic DNA. Translation: AAD42469.1.
PIRiA04302. IDBPA4.
RefSeqiNP_049665.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3U5ZX-ray3.50B/C/D/E/L/M/N/O1-319[»]
3U60X-ray3.34B/C/D/E1-319[»]
3U61X-ray3.20B/C/D/E1-319[»]
ProteinModelPortaliP04526.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258787.
KEGGivg:1258787.

Phylogenomic databases

KOiK18944.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPA44_BPT4
AccessioniPrimary (citable) accession number: P04526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: June 8, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.