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P04525 (DPA5_BPT4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase processivity component
Alternative name(s):
DNA polymerase accessory protein 45
Gp45
Gene names
Name:45
OrganismEnterobacteria phage T4 (Bacteriophage T4) [Reference proteome]
Taxonomic identifier10665 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Replisome sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.

Subunit structure

Homotrimer.

Ontologies

Keywords
   Biological processDNA replication
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 228228DNA polymerase processivity component
PRO_0000149229

Experimental info

Sequence conflict691Missing in CAA24777. Ref.1
Sequence conflict931P → R in CAA24777. Ref.1
Sequence conflict931P → R in CAA25340. Ref.1
Sequence conflict931P → R in AAC05393. Ref.1
Sequence conflict1001V → D in CAA24777. Ref.1
Sequence conflict1291V → L in CAA24777. Ref.1

Secondary structure

............................................ 228
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04525 [UniParc].

Last modified December 19, 2001. Version 3.
Checksum: 765DF4AAB76D005B

FASTA22824,858
        10         20         30         40         50         60 
MKLSKDTTAL LKNFATINSG IMLKSGQFIM TRAVNGTTYA EANISDVIDF DVAIYDLNGF 

        70         80         90        100        110        120 
LGILSLVNDD AEISQSEDGN IKIADARSTI FWPAADPSTV VAPNKPIPFP VASAVTEIKA 

       130        140        150        160        170        180 
EDLQQLLRVS RGLQIDTIAI TVKEGKIVIN GFNKVEDSAL TRVKYSLTLG DYDGENTFNF 

       190        200        210        220 
IINMANMKMQ PGNYKLLLWA KGKQGAAKFE GEHANYVVAL EADSTHDF 

« Hide

References

« Hide 'large scale' references
[1]"Bacteriophage T4 gene 45. Sequences of the structural gene and its protein product."
Spicer E.K., Noble J.A., Nossal N.G., Konigsberg W.H., Williams K.R.
J. Biol. Chem. 257:8972-8979(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Spicer E.K.
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 69; 100 AND 129.
[3]"Bacteriophage T4 genome."
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.
Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Identification of two new bacteriophage T4 genes that may have roles in transcription and DNA replication."
Hsu T., Wei R., Dawson M., Karam J.D.
J. Virol. 61:366-374(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36.
[5]"Crystal structure of the DNA polymerase processivity factor of T4 bacteriophage."
Moarefi I., Jeruzalmi D., Turner J., O'Donnell M., Kuriyan J.
J. Mol. Biol. 296:1215-1223(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V01535 Genomic DNA. Translation: CAA24777.1.
M10160 Genomic DNA. Translation: AAC05393.1.
X00769 Genomic DNA. Translation: CAA25340.1.
AF158101 Genomic DNA. Translation: AAD42470.1.
M15080 Genomic DNA. Translation: AAA32519.1.
PIRIDBP44. A04301.
RefSeqNP_049666.1. NC_000866.4.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CZDX-ray2.45A/B/C1-228[»]
3U5ZX-ray3.50F/G/H/P/Q/R1-228[»]
3U60X-ray3.34F/G/H1-228[»]
3U61X-ray3.20F/G/H1-228[»]
ProteinModelPortalP04525.
SMRP04525. Positions 1-228.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1258821.

Family and domain databases

InterProIPR004190. DNA_pol_proc_fac.
IPR015200. Gp45_slide_clamp_C.
[Graphical view]
PfamPF02916. DNA_PPF. 1 hit.
PF09116. gp45-slide_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP04525.

Entry information

Entry nameDPA5_BPT4
AccessionPrimary (citable) accession number: P04525
Secondary accession number(s): Q9T0Q0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: December 19, 2001
Last modified: May 14, 2014
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references