Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P04525

- DPA5_BPT4

UniProt

P04525 - DPA5_BPT4

Protein

DNA polymerase processivity component

Gene

45

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 3 (19 Dec 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Replisome sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Biological processi

    DNA replication

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase processivity component
    Alternative name(s):
    DNA polymerase accessory protein 45
    Gp45
    Gene namesi
    Name:45
    OrganismiEnterobacteria phage T4 (Bacteriophage T4)
    Taxonomic identifieri10665 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000009087: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 228228DNA polymerase processivity componentPRO_0000149229Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.

    Structurei

    Secondary structure

    1
    228
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 1713
    Beta strandi21 – 233
    Beta strandi25 – 328
    Beta strandi36 – 4813
    Beta strandi52 – 554
    Helixi57 – 648
    Beta strandi72 – 754
    Beta strandi79 – 846
    Beta strandi86 – 927
    Helixi97 – 993
    Beta strandi113 – 1186
    Helixi120 – 13213
    Beta strandi137 – 1437
    Beta strandi146 – 1527
    Helixi154 – 1563
    Beta strandi158 – 1603
    Beta strandi165 – 1717
    Beta strandi178 – 1836
    Helixi184 – 1863
    Beta strandi194 – 2018
    Beta strandi204 – 2107
    Beta strandi212 – 2198

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CZDX-ray2.45A/B/C1-228[»]
    3U5ZX-ray3.50F/G/H/P/Q/R1-228[»]
    3U60X-ray3.34F/G/H1-228[»]
    3U61X-ray3.20F/G/H1-228[»]
    ProteinModelPortaliP04525.
    SMRiP04525. Positions 1-228.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04525.

    Family & Domainsi

    Family and domain databases

    InterProiIPR004190. DNA_pol_proc_fac.
    IPR015200. Gp45_slide_clamp_C.
    [Graphical view]
    PfamiPF02916. DNA_PPF. 1 hit.
    PF09116. gp45-slide_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04525-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLSKDTTAL LKNFATINSG IMLKSGQFIM TRAVNGTTYA EANISDVIDF    50
    DVAIYDLNGF LGILSLVNDD AEISQSEDGN IKIADARSTI FWPAADPSTV 100
    VAPNKPIPFP VASAVTEIKA EDLQQLLRVS RGLQIDTIAI TVKEGKIVIN 150
    GFNKVEDSAL TRVKYSLTLG DYDGENTFNF IINMANMKMQ PGNYKLLLWA 200
    KGKQGAAKFE GEHANYVVAL EADSTHDF 228
    Length:228
    Mass (Da):24,858
    Last modified:December 19, 2001 - v3
    Checksum:i765DF4AAB76D005B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti69 – 691Missing in CAA24777. (PubMed:6284751)Curated
    Sequence conflicti93 – 931P → R in CAA24777. (PubMed:6284751)Curated
    Sequence conflicti93 – 931P → R in CAA25340. (PubMed:6284751)Curated
    Sequence conflicti93 – 931P → R in AAC05393. (PubMed:6284751)Curated
    Sequence conflicti100 – 1001V → D in CAA24777. (PubMed:6284751)Curated
    Sequence conflicti129 – 1291V → L in CAA24777. (PubMed:6284751)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01535 Genomic DNA. Translation: CAA24777.1.
    M10160 Genomic DNA. Translation: AAC05393.1.
    X00769 Genomic DNA. Translation: CAA25340.1.
    AF158101 Genomic DNA. Translation: AAD42470.1.
    M15080 Genomic DNA. Translation: AAA32519.1.
    PIRiA04301. IDBP44.
    RefSeqiNP_049666.1. NC_000866.4.

    Genome annotation databases

    GeneIDi1258821.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01535 Genomic DNA. Translation: CAA24777.1 .
    M10160 Genomic DNA. Translation: AAC05393.1 .
    X00769 Genomic DNA. Translation: CAA25340.1 .
    AF158101 Genomic DNA. Translation: AAD42470.1 .
    M15080 Genomic DNA. Translation: AAA32519.1 .
    PIRi A04301. IDBP44.
    RefSeqi NP_049666.1. NC_000866.4.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CZD X-ray 2.45 A/B/C 1-228 [» ]
    3U5Z X-ray 3.50 F/G/H/P/Q/R 1-228 [» ]
    3U60 X-ray 3.34 F/G/H 1-228 [» ]
    3U61 X-ray 3.20 F/G/H 1-228 [» ]
    ProteinModelPortali P04525.
    SMRi P04525. Positions 1-228.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1258821.

    Miscellaneous databases

    EvolutionaryTracei P04525.

    Family and domain databases

    InterProi IPR004190. DNA_pol_proc_fac.
    IPR015200. Gp45_slide_clamp_C.
    [Graphical view ]
    Pfami PF02916. DNA_PPF. 1 hit.
    PF09116. gp45-slide_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bacteriophage T4 gene 45. Sequences of the structural gene and its protein product."
      Spicer E.K., Noble J.A., Nossal N.G., Konigsberg W.H., Williams K.R.
      J. Biol. Chem. 257:8972-8979(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Spicer E.K.
      Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 69; 100 AND 129.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Identification of two new bacteriophage T4 genes that may have roles in transcription and DNA replication."
      Hsu T., Wei R., Dawson M., Karam J.D.
      J. Virol. 61:366-374(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36.
    5. "Crystal structure of the DNA polymerase processivity factor of T4 bacteriophage."
      Moarefi I., Jeruzalmi D., Turner J., O'Donnell M., Kuriyan J.
      J. Mol. Biol. 296:1215-1223(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS).

    Entry informationi

    Entry nameiDPA5_BPT4
    AccessioniPrimary (citable) accession number: P04525
    Secondary accession number(s): Q9T0Q0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: December 19, 2001
    Last modified: October 1, 2014
    This is version 79 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3