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Protein

DNA polymerase clamp

Gene

45

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Replisome processivity clamp subunit that increases the processivity of DNA synthesis. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.1 Publication

GO - Biological processi

  • bidirectional double-stranded viral DNA replication Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA replication, Viral DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase clamp1 Publication
Alternative name(s):
DNA polymerase accessory protein 45
DNA polymerase sliding clamp1 Publication
Gp45
Gene namesi
Name:45
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492291 – 228DNA polymerase clampAdd BLAST228

Interactioni

Subunit structurei

Homotrimer (PubMed:26735934). Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the helicase and the helicase assembly factor (PubMed:16800624, PubMed:26102578). Interacts with the DNA polymerase; this interaction constitutes the polymerase holoenzyme (PubMed:8475061).4 Publications

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 17Combined sources13
Beta strandi21 – 23Combined sources3
Beta strandi25 – 32Combined sources8
Beta strandi36 – 48Combined sources13
Beta strandi52 – 55Combined sources4
Helixi57 – 64Combined sources8
Beta strandi72 – 75Combined sources4
Beta strandi79 – 84Combined sources6
Beta strandi86 – 92Combined sources7
Helixi97 – 99Combined sources3
Beta strandi113 – 118Combined sources6
Helixi120 – 132Combined sources13
Beta strandi137 – 143Combined sources7
Beta strandi146 – 152Combined sources7
Helixi154 – 156Combined sources3
Beta strandi158 – 160Combined sources3
Beta strandi165 – 171Combined sources7
Beta strandi178 – 183Combined sources6
Helixi184 – 186Combined sources3
Beta strandi194 – 201Combined sources8
Beta strandi204 – 210Combined sources7
Beta strandi212 – 219Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CZDX-ray2.45A/B/C1-228[»]
3U5ZX-ray3.50F/G/H/P/Q/R1-228[»]
3U60X-ray3.34F/G/H1-228[»]
3U61X-ray3.20F/G/H1-228[»]
ProteinModelPortaliP04525.
SMRiP04525.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04525.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK18943.

Family and domain databases

InterProiIPR004190. DNA_pol_proc_fac.
IPR015200. Gp45_slide_clamp_C.
[Graphical view]
PfamiPF02916. DNA_PPF. 1 hit.
PF09116. gp45-slide_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04525-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSKDTTAL LKNFATINSG IMLKSGQFIM TRAVNGTTYA EANISDVIDF
60 70 80 90 100
DVAIYDLNGF LGILSLVNDD AEISQSEDGN IKIADARSTI FWPAADPSTV
110 120 130 140 150
VAPNKPIPFP VASAVTEIKA EDLQQLLRVS RGLQIDTIAI TVKEGKIVIN
160 170 180 190 200
GFNKVEDSAL TRVKYSLTLG DYDGENTFNF IINMANMKMQ PGNYKLLLWA
210 220
KGKQGAAKFE GEHANYVVAL EADSTHDF
Length:228
Mass (Da):24,858
Last modified:December 19, 2001 - v3
Checksum:i765DF4AAB76D005B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69Missing in CAA24777 (PubMed:6284751).Curated1
Sequence conflicti93P → R in CAA24777 (PubMed:6284751).Curated1
Sequence conflicti93P → R in CAA25340 (PubMed:6284751).Curated1
Sequence conflicti93P → R in AAC05393 (PubMed:6284751).Curated1
Sequence conflicti100V → D in CAA24777 (PubMed:6284751).Curated1
Sequence conflicti129V → L in CAA24777 (PubMed:6284751).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01535 Genomic DNA. Translation: CAA24777.1.
M10160 Genomic DNA. Translation: AAC05393.1.
X00769 Genomic DNA. Translation: CAA25340.1.
AF158101 Genomic DNA. Translation: AAD42470.1.
M15080 Genomic DNA. Translation: AAA32519.1.
PIRiA04301. IDBP44.
RefSeqiNP_049666.1. NC_000866.4.

Genome annotation databases

GeneIDi1258821.
KEGGivg:1258821.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01535 Genomic DNA. Translation: CAA24777.1.
M10160 Genomic DNA. Translation: AAC05393.1.
X00769 Genomic DNA. Translation: CAA25340.1.
AF158101 Genomic DNA. Translation: AAD42470.1.
M15080 Genomic DNA. Translation: AAA32519.1.
PIRiA04301. IDBP44.
RefSeqiNP_049666.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CZDX-ray2.45A/B/C1-228[»]
3U5ZX-ray3.50F/G/H/P/Q/R1-228[»]
3U60X-ray3.34F/G/H1-228[»]
3U61X-ray3.20F/G/H1-228[»]
ProteinModelPortaliP04525.
SMRiP04525.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258821.
KEGGivg:1258821.

Phylogenomic databases

KOiK18943.

Miscellaneous databases

EvolutionaryTraceiP04525.

Family and domain databases

InterProiIPR004190. DNA_pol_proc_fac.
IPR015200. Gp45_slide_clamp_C.
[Graphical view]
PfamiPF02916. DNA_PPF. 1 hit.
PF09116. gp45-slide_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPA5_BPT4
AccessioniPrimary (citable) accession number: P04525
Secondary accession number(s): Q9T0Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: December 19, 2001
Last modified: November 2, 2016
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.